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The map label for this gene is ppk

Identifier: 170023586

GI number: 170023586

Start: 1481475

End: 1483538

Strand: Reverse

Name: ppk

Synonym: YPK_1344

Alternate gene names: 170023586

Gene position: 1483538-1481475 (Counterclockwise)

Preceding gene: 170023591

Following gene: 170023585

Centisome position: 31.64

GC content: 44.33

Gene sequence:

>2064_bases
ATGGGTCAGGAAAAGCTCTATACCGAAAAAGAACTCAGTTGGTTATCTTTTAATGAACGGGTATTGCAGGAAGCGGCGGA
TAAGAGCAACCCGCTTATTGAGCGGATGCGATTTCTTGGCATTTACTCCAATAATCTGGATGAGTTTTATAAAGTTCGTT
TTGCCGATCTGAAACGGCGCATTCTTATTAACGAAGAGCAAGGCTCTGCTGTGACGTCCCGCCATTTGCTGAAAAAAATT
CAGAGCAAAGTGGTGAAAGCCGATCAAGAGTTTGATGGGCTGTATAATGACCTGTTACTGGAAATGGCCCGTAATCAAAT
CTTTTTGGTTAATGAGCGGCAAATTTCCGAGAATCAGCAAACGTGGCTGAAGCAATACTTTAAACAGCATCTGCGTCAAC
ACATTACACCGATCCTGATTAACCATGACACCAATCTGGTTCAGTTTTTAAAAGATGATTATACCTATCTGGCGGTAGAA
ATTATTCGTGGGCAGGATATTGCCTATGCCCTGCTGGAAATTCCATCAGATAAAATACCGCGCTTTGTCAATTTACCGCC
TGAAGCGCCTCGCCGTCGCAAGCCAATGATATTACTTGATAATATATTGCGCTTCTGTCTGGATGAAATATTTAAAGGCT
TCTTTGATTATGATGCGCTGAATGCATATTCAATGAAAATGACGCGGGATGCCGAATATGATTTGGTGACCGAGATGGAA
TCAAGCCTCTTGGAATTGATGTCATCTAGTTTGAAACAGCGTCTGACAGCCGAACCCGTGCGTTTTGTTTACCAGCGGGA
TATGCCCGATGAAATGGTAGAACTCCTGCGCAATAAATTGGGTATATCTAACGATGATTCCGTGATCGCCGGTGGCCGTT
ACCATAATTTCAAAGACTTTATCAACTTCCCCAATGTAGGCAAAAGTAATCTGGTTAACCGGCCAATGCCACGCCTGCGC
CATGTCTGGTTTGATAAATTCCGCAACGGTTTTGATGCCATCCGCGAACAGGATGTGCTGCTGTACTACCCGTATCATAC
CTTTGAGCATGTGCTGGAGTTATTACGGCAAGCCTCATTCGATCCAAGCGTTCTGGCGATTAAAATCAATATTTATCGGG
TCGCCAAAGATTCACGCATTATCGACTCGATGATCCATGCCGCTCATAACGGCAAAAAGGTAACGGTGGTCGTGGAGTTG
CAGGCTCGCTTTGATGAAGAGGCCAATATTCATTGGGCTAAAAGTCTGACGGCGGCTGGGGTACACGTTATTTTCTCTGC
GCCAGGGCTGAAAATTCATGCCAAACTGTTCCTGATTTCTCGCCTTGAAAATGACGAAATCGTGCGTTATGCCCATATTG
GCACCGGTAACTTTAACGAGAAGACCGCCAGAATTTATACCGACTATTCGTTGCTGACGGCAGATGCCAGGATTACCAAC
GAAGTGCGCCGGGTATTTAACTTTATTGAAAATCCCTATCGCCCGGTTACGTTCGATAATCTGATGGTTTCGCCACAGAA
TTCGCGCTTAATATTATATCAGTTGATTGATCAGGAAATTATCCACGCACAGGCCGGTGAAAGCGCAGGAATTACCCTAA
AAATAAATAATTTAGTGGATAAAGGCCTGGTAGATAGGTTATATAGCGCCTCCAGTGCTGGCGTCAAAATACGTCTATTG
GTACGCGGTATGTGTTCATTAATCCCGAATATGCCGGGTATCAGCGATAATATTCAGGTCACCAGTATTGTTGATCGCTT
TTTAGAGCATGACCGTGTGTACGTCTTTGAGAATAAAGGCGATAAACTGGTTTATCTCTCCTCTGCGGACTGGATGACCC
GTAATATTGACTACCGAATTGAAGTGGCTGTTTCGCTGTTGGATCCAAAGTTGAAGCAGCGGGTGCTGGATATCTTAGAG
ATATTATTCAACGACACCGTCAAAGCCCGTTATATTGATAAAGAGCTGAGTAATCGTTATGTACCGCGTGGAAACCGGCG
CAAAGTACGTGCACAGATTGCTATCTATGATTATTTAAAAGCACTGGAACAACCGGAGCAGTAG

Upstream 100 bases:

>100_bases
ACAATGTTAACTAATGATATCTGTTGGACTTTTTTGTCAATTTTTCGCAATAATGTTTACGGACACTAAATACTCCCACA
ATAATCTTAATGGAGTTGCA

Downstream 100 bases:

>100_bases
ACCTAACACTATGCCGCTAACCAATAATTCGACTACCAAACCGCAGGAAATTGCCGCCATCGATCTTGGCTCTAACAGTT
TCCATATGGTCATTGCCCGT

Product: polyphosphate kinase

Products: NA

Alternate protein names: ATP-polyphosphate phosphotransferase; Polyphosphoric acid kinase

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MGQEKLYTEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSNNLDEFYKVRFADLKRRILINEEQGSAVTSRHLLKKI
QSKVVKADQEFDGLYNDLLLEMARNQIFLVNERQISENQQTWLKQYFKQHLRQHITPILINHDTNLVQFLKDDYTYLAVE
IIRGQDIAYALLEIPSDKIPRFVNLPPEAPRRRKPMILLDNILRFCLDEIFKGFFDYDALNAYSMKMTRDAEYDLVTEME
SSLLELMSSSLKQRLTAEPVRFVYQRDMPDEMVELLRNKLGISNDDSVIAGGRYHNFKDFINFPNVGKSNLVNRPMPRLR
HVWFDKFRNGFDAIREQDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHNGKKVTVVVEL
QARFDEEANIHWAKSLTAAGVHVIFSAPGLKIHAKLFLISRLENDEIVRYAHIGTGNFNEKTARIYTDYSLLTADARITN
EVRRVFNFIENPYRPVTFDNLMVSPQNSRLILYQLIDQEIIHAQAGESAGITLKINNLVDKGLVDRLYSASSAGVKIRLL
VRGMCSLIPNMPGISDNIQVTSIVDRFLEHDRVYVFENKGDKLVYLSSADWMTRNIDYRIEVAVSLLDPKLKQRVLDILE
ILFNDTVKARYIDKELSNRYVPRGNRRKVRAQIAIYDYLKALEQPEQ

Sequences:

>Translated_687_residues
MGQEKLYTEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSNNLDEFYKVRFADLKRRILINEEQGSAVTSRHLLKKI
QSKVVKADQEFDGLYNDLLLEMARNQIFLVNERQISENQQTWLKQYFKQHLRQHITPILINHDTNLVQFLKDDYTYLAVE
IIRGQDIAYALLEIPSDKIPRFVNLPPEAPRRRKPMILLDNILRFCLDEIFKGFFDYDALNAYSMKMTRDAEYDLVTEME
SSLLELMSSSLKQRLTAEPVRFVYQRDMPDEMVELLRNKLGISNDDSVIAGGRYHNFKDFINFPNVGKSNLVNRPMPRLR
HVWFDKFRNGFDAIREQDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHNGKKVTVVVEL
QARFDEEANIHWAKSLTAAGVHVIFSAPGLKIHAKLFLISRLENDEIVRYAHIGTGNFNEKTARIYTDYSLLTADARITN
EVRRVFNFIENPYRPVTFDNLMVSPQNSRLILYQLIDQEIIHAQAGESAGITLKINNLVDKGLVDRLYSASSAGVKIRLL
VRGMCSLIPNMPGISDNIQVTSIVDRFLEHDRVYVFENKGDKLVYLSSADWMTRNIDYRIEVAVSLLDPKLKQRVLDILE
ILFNDTVKARYIDKELSNRYVPRGNRRKVRAQIAIYDYLKALEQPEQ
>Mature_686_residues
GQEKLYTEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSNNLDEFYKVRFADLKRRILINEEQGSAVTSRHLLKKIQ
SKVVKADQEFDGLYNDLLLEMARNQIFLVNERQISENQQTWLKQYFKQHLRQHITPILINHDTNLVQFLKDDYTYLAVEI
IRGQDIAYALLEIPSDKIPRFVNLPPEAPRRRKPMILLDNILRFCLDEIFKGFFDYDALNAYSMKMTRDAEYDLVTEMES
SLLELMSSSLKQRLTAEPVRFVYQRDMPDEMVELLRNKLGISNDDSVIAGGRYHNFKDFINFPNVGKSNLVNRPMPRLRH
VWFDKFRNGFDAIREQDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHNGKKVTVVVELQ
ARFDEEANIHWAKSLTAAGVHVIFSAPGLKIHAKLFLISRLENDEIVRYAHIGTGNFNEKTARIYTDYSLLTADARITNE
VRRVFNFIENPYRPVTFDNLMVSPQNSRLILYQLIDQEIIHAQAGESAGITLKINNLVDKGLVDRLYSASSAGVKIRLLV
RGMCSLIPNMPGISDNIQVTSIVDRFLEHDRVYVFENKGDKLVYLSSADWMTRNIDYRIEVAVSLLDPKLKQRVLDILEI
LFNDTVKARYIDKELSNRYVPRGNRRKVRAQIAIYDYLKALEQPEQ

Specific function: Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP)

COG id: COG0855

COG function: function code P; Polyphosphate kinase

Gene ontology:

Cell location: Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PLD phosphodiesterase domain

Homologues:

Organism=Escherichia coli, GI1788847, Length=688, Percent_Identity=86.9186046511628, Blast_Score=1242, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPK_YERPE (Q8ZCX2)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AE0345
- RefSeq:   NP_668719.1
- RefSeq:   NP_994015.1
- RefSeq:   YP_002347774.1
- ProteinModelPortal:   Q8ZCX2
- SMR:   Q8ZCX2
- GeneID:   1146345
- GeneID:   1175661
- GeneID:   2766112
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO2836
- KEGG:   ypk:y1398
- KEGG:   ypm:YP_2703
- HOGENOM:   HBG341268
- OMA:   FLISRRE
- ProtClustDB:   PRK05443
- BioCyc:   YPES187410:Y1398-MONOMER
- BioCyc:   YPES214092:YPO2836-MONOMER
- BRENDA:   2.7.4.1
- HAMAP:   MF_00347
- InterPro:   IPR001736
- InterPro:   IPR003414
- PIRSF:   PIRSF015589
- TIGRFAMs:   TIGR03705

Pfam domain/function: PF02503 PP_kinase

EC number: =2.7.4.1

Molecular weight: Translated: 80016; Mature: 79885

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 433-433 ACT_SITE 452-452

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQEKLYTEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSNNLDEFYKVRFADLKRR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ILINEEQGSAVTSRHLLKKIQSKVVKADQEFDGLYNDLLLEMARNQIFLVNERQISENQQ
EEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECHHHCCHHH
TWLKQYFKQHLRQHITPILINHDTNLVQFLKDDYTYLAVEIIRGQDIAYALLEIPSDKIP
HHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCEEEEEEEECCCCCEEHEEECCCHHCC
RFVNLPPEAPRRRKPMILLDNILRFCLDEIFKGFFDYDALNAYSMKMTRDAEYDLVTEME
CEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHH
SSLLELMSSSLKQRLTAEPVRFVYQRDMPDEMVELLRNKLGISNDDSVIAGGRYHNFKDF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCHHHH
INFPNVGKSNLVNRPMPRLRHVWFDKFRNGFDAIREQDVLLYYPYHTFEHVLELLRQASF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHCCCCEEEEECHHHHHHHHHHHHHCCC
DPSVLAIKINIYRVAKDSRIIDSMIHAAHNGKKVTVVVELQARFDEEANIHWAKSLTAAG
CCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEHHHHHHHCC
VHVIFSAPGLKIHAKLFLISRLENDEIVRYAHIGTGNFNEKTARIYTDYSLLTADARITN
EEEEEECCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCCEEEEEECHHEEECCHHHHH
EVRRVFNFIENPYRPVTFDNLMVSPQNSRLILYQLIDQEIIHAQAGESAGITLKINNLVD
HHHHHHHHHCCCCCCEEECCEEECCCCCCEEHHHHHHHHHHHHCCCCCCCEEEEEHHHHH
KGLVDRLYSASSAGVKIRLLVRGMCSLIPNMPGISDNIQVTSIVDRFLEHDRVYVFENKG
HHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCC
DKLVYLSSADWMTRNIDYRIEVAVSLLDPKLKQRVLDILEILFNDTVKARYIDKELSNRY
CEEEEEECCCCEECCCCEEEEEEHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
VPRGNRRKVRAQIAIYDYLKALEQPEQ
CCCCCCCEEEHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GQEKLYTEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSNNLDEFYKVRFADLKRR
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ILINEEQGSAVTSRHLLKKIQSKVVKADQEFDGLYNDLLLEMARNQIFLVNERQISENQQ
EEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECHHHCCHHH
TWLKQYFKQHLRQHITPILINHDTNLVQFLKDDYTYLAVEIIRGQDIAYALLEIPSDKIP
HHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCEEEEEEEECCCCCEEHEEECCCHHCC
RFVNLPPEAPRRRKPMILLDNILRFCLDEIFKGFFDYDALNAYSMKMTRDAEYDLVTEME
CEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHH
SSLLELMSSSLKQRLTAEPVRFVYQRDMPDEMVELLRNKLGISNDDSVIAGGRYHNFKDF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCHHHH
INFPNVGKSNLVNRPMPRLRHVWFDKFRNGFDAIREQDVLLYYPYHTFEHVLELLRQASF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHCCCCEEEEECHHHHHHHHHHHHHCCC
DPSVLAIKINIYRVAKDSRIIDSMIHAAHNGKKVTVVVELQARFDEEANIHWAKSLTAAG
CCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEHHHHHHHCC
VHVIFSAPGLKIHAKLFLISRLENDEIVRYAHIGTGNFNEKTARIYTDYSLLTADARITN
EEEEEECCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCCEEEEEECHHEEECCHHHHH
EVRRVFNFIENPYRPVTFDNLMVSPQNSRLILYQLIDQEIIHAQAGESAGITLKINNLVD
HHHHHHHHHCCCCCCEEECCEEECCCCCCEEHHHHHHHHHHHHCCCCCCCEEEEEHHHHH
KGLVDRLYSASSAGVKIRLLVRGMCSLIPNMPGISDNIQVTSIVDRFLEHDRVYVFENKG
HHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCC
DKLVYLSSADWMTRNIDYRIEVAVSLLDPKLKQRVLDILEILFNDTVKARYIDKELSNRY
CEEEEEECCCCEECCCCEEEEEEHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
VPRGNRRKVRAQIAIYDYLKALEQPEQ
CCCCCCCEEEHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430