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The map label for this gene is yafV [C]

Identifier: 170022722

GI number: 170022722

Start: 515843

End: 516712

Strand: Direct

Name: yafV [C]

Synonym: YPK_0471

Alternate gene names: 170022722

Gene position: 515843-516712 (Clockwise)

Preceding gene: 170022721

Following gene: 170022723

Centisome position: 11.0

GC content: 50.11

Gene sequence:

>870_bases
ATGAAAAATGCCAATGTTGCATTATTGCAGTTGTGCAGCGGTGAAAATACCCGTGACAATCTGGCTCAGATTGAGCAGCA
GATCAAACAGTTAAATGCAGGTATCAAACTGGTTATGACGCCTGAGAATGCGTTGTTATTTGCTAATGCTGCCTCTTATC
GTCACCATGCGGAACACCATAATGACGGCCCATTACAGCAAGAAGTGCGTGAAATGGCACGGCGCTACGGGGTTTGGATA
CAGGTTGGCTCCATGCCGATGGTGAGCCGTGAGTCACCGGACCTGATCACCAGCAGTAGCTTGTTATTTGACGATCAAGG
CGAACTCAAAGCCCGTTACGATAAAATTCACATGTTTGATGTCGATATCAATGATATCCATGGCCATTACCGTGAGTCTG
ATACTTATCAGCCGGGGCAGCAGCTGACGGTGGTTGATACCCCGGTTGGCCGGTTGGGCATGACCATTTGCTATGATTTG
CGTTTCCCTGGCTTGTTTCAGGCTTTGCGAGCGCAGGGGGCTGAAATCATTTCTGTTCCGGCAGCCTTTACCAAAATGAC
GGGTGAGGCGCATTGGGAAACATTGCTCCGTGCACGGGCTATCGAAAACCAGTGCGTGATATTGGCTGCCGCACAGGTGG
GGCGCCACGGTGCTACCCGCCGGACGTGGGGTCATACCATGGCAGTCGATGCCTGGGGTAAAATTCTGGGGCATAACCCA
GATGCGGTGGCGGCACTAAAAGTAAGAATTGAAACTGCAGGTTTGAAGACGATTCGCAATCAAATGCCGGTCATGCAGCA
CAATCGCTTTCAGGCGACGCTGGAGCCGACATTGAATAAACCATTATTGAATAAACCACCATCTAATTAA

Upstream 100 bases:

>100_bases
TATGGAGCAAAGTTTCGCTGATCCGCTATCAGATTACCGGTAGTCTGGATCAACCGACCATCCATGAAGTGTTACGTCAG
CTAAAGGAGAATGAGGCGCC

Downstream 100 bases:

>100_bases
GAGTAAAAACTATGAGCCTCTCATTTGTCAGTGAGCAGTTACTCACTGCTAACAAGTTGAACCATGAGGACTTGTTCTCA
GTGTTGGGGCAGCTAACTGA

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKNANVALLQLCSGENTRDNLAQIEQQIKQLNAGIKLVMTPENALLFANAASYRHHAEHHNDGPLQQEVREMARRYGVWI
QVGSMPMVSRESPDLITSSSLLFDDQGELKARYDKIHMFDVDINDIHGHYRESDTYQPGQQLTVVDTPVGRLGMTICYDL
RFPGLFQALRAQGAEIISVPAAFTKMTGEAHWETLLRARAIENQCVILAAAQVGRHGATRRTWGHTMAVDAWGKILGHNP
DAVAALKVRIETAGLKTIRNQMPVMQHNRFQATLEPTLNKPLLNKPPSN

Sequences:

>Translated_289_residues
MKNANVALLQLCSGENTRDNLAQIEQQIKQLNAGIKLVMTPENALLFANAASYRHHAEHHNDGPLQQEVREMARRYGVWI
QVGSMPMVSRESPDLITSSSLLFDDQGELKARYDKIHMFDVDINDIHGHYRESDTYQPGQQLTVVDTPVGRLGMTICYDL
RFPGLFQALRAQGAEIISVPAAFTKMTGEAHWETLLRARAIENQCVILAAAQVGRHGATRRTWGHTMAVDAWGKILGHNP
DAVAALKVRIETAGLKTIRNQMPVMQHNRFQATLEPTLNKPLLNKPPSN
>Mature_289_residues
MKNANVALLQLCSGENTRDNLAQIEQQIKQLNAGIKLVMTPENALLFANAASYRHHAEHHNDGPLQQEVREMARRYGVWI
QVGSMPMVSRESPDLITSSSLLFDDQGELKARYDKIHMFDVDINDIHGHYRESDTYQPGQQLTVVDTPVGRLGMTICYDL
RFPGLFQALRAQGAEIISVPAAFTKMTGEAHWETLLRARAIENQCVILAAAQVGRHGATRRTWGHTMAVDAWGKILGHNP
DAVAALKVRIETAGLKTIRNQMPVMQHNRFQATLEPTLNKPLLNKPPSN

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=268, Percent_Identity=36.1940298507463, Blast_Score=178, Evalue=6e-45,
Organism=Homo sapiens, GI297632348, Length=268, Percent_Identity=36.1940298507463, Blast_Score=177, Evalue=7e-45,
Organism=Homo sapiens, GI297632350, Length=268, Percent_Identity=36.1940298507463, Blast_Score=177, Evalue=9e-45,
Organism=Homo sapiens, GI9910460, Length=272, Percent_Identity=31.6176470588235, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI297632346, Length=192, Percent_Identity=32.8125, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17556280, Length=268, Percent_Identity=35.0746268656716, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322335, Length=300, Percent_Identity=33.6666666666667, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6323383, Length=274, Percent_Identity=30.2919708029197, Blast_Score=135, Evalue=5e-33,
Organism=Drosophila melanogaster, GI17933642, Length=295, Percent_Identity=35.2542372881356, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI21355835, Length=274, Percent_Identity=27.7372262773723, Blast_Score=142, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 32273; Mature: 32273

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNANVALLQLCSGENTRDNLAQIEQQIKQLNAGIKLVMTPENALLFANAASYRHHAEHH
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEECHHHHHHHCCC
NDGPLQQEVREMARRYGVWIQVGSMPMVSRESPDLITSSSLLFDDQGELKARYDKIHMFD
CCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEECCCEEECCCCCCEEEEEEEEEEE
VDINDIHGHYRESDTYQPGQQLTVVDTPVGRLGMTICYDLRFPGLFQALRAQGAEIISVP
CCHHHHCCCCCCCCCCCCCCEEEEEECCCCHHCCEEEEECCCCHHHHHHHHCCCEEEECC
AAFTKMTGEAHWETLLRARAIENQCVILAAAQVGRHGATRRTWGHTMAVDAWGKILGHNP
HHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCCCCCCEEEHHHHHHHHCCCC
DAVAALKVRIETAGLKTIRNQMPVMQHNRFQATLEPTLNKPLLNKPPSN
CCEEEEEEEEEHHHHHHHHHCCCCHHCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNANVALLQLCSGENTRDNLAQIEQQIKQLNAGIKLVMTPENALLFANAASYRHHAEHH
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEECHHHHHHHCCC
NDGPLQQEVREMARRYGVWIQVGSMPMVSRESPDLITSSSLLFDDQGELKARYDKIHMFD
CCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEECCCEEECCCCCCEEEEEEEEEEE
VDINDIHGHYRESDTYQPGQQLTVVDTPVGRLGMTICYDLRFPGLFQALRAQGAEIISVP
CCHHHHCCCCCCCCCCCCCCEEEEEECCCCHHCCEEEEECCCCHHHHHHHHCCCEEEECC
AAFTKMTGEAHWETLLRARAIENQCVILAAAQVGRHGATRRTWGHTMAVDAWGKILGHNP
HHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCCCCCCEEEHHHHHHHHCCCC
DAVAALKVRIETAGLKTIRNQMPVMQHNRFQATLEPTLNKPLLNKPPSN
CCEEEEEEEEEHHHHHHHHHCCCCHHCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]