Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 170022586

GI number: 170022586

Start: 350676

End: 351635

Strand: Direct

Name: birA [H]

Synonym: YPK_0327

Alternate gene names: 170022586

Gene position: 350676-351635 (Clockwise)

Preceding gene: 170022585

Following gene: 170022590

Centisome position: 7.48

GC content: 40.62

Gene sequence:

>960_bases
GTGAAAGATATCAGGGTCCCCTTACGGTTAGTCAGTATATTATCTGATGGTTTTTTTCACTCCGGTGAGCAATTGGGTGA
AACGTTAGGCATGAGTCGCGCTGCGATTAATAAACACATGCAAACTATTAGAGATTGGGGTTTAGATGTTTTTACTGTCC
CAGGTAAGGGCTACAGCTTACCTACTTCTATCCAATTGCTTGATGAACAAAAAATATTAAGTTATTTACCTACGGGTCAC
GTAACAGTATTACCCGTAGTCGATTCTACTAATCAATATTTATTGGATCGTATTACTGAATTGAAATCAGGTGATGCGTG
TGTTGCGGAATATCAGCAAGCAGGACGAGGTCGGCGTGGGCGTCAATGGGTTTCTCCTTTTGGTGCTAATCTTTATTTAT
CAATGTATTGGCGGCTAGAGCAAGGTCCTGCTGCTGCAATGGGATTAAGCTTGGTTGTTGGGATTGTTATGGCAGAGGTT
CTGCAGAATTTAGGTGCAGAGAAGGTTCGTGTTAAGTGGCCTAATGATCTGTATTTAAATGATAAAAAGCTGGCCGGAAT
ATTAGTAGAGCTTACTGGGAAAACGGGTGATGCTGCACAATTAGTCATTGGTGCAGGTATTAACCTGACAATGCGTGGTT
CAGAAACAAATGCTATTAACCAAGATTGGATAAATTTGCAGGATGCTGGTGTTACTATCGATCGTAATAAACTCACTGCT
GAGATCTTATCTGAATTACGGCTTGCAGTAGTTAAGTTCGAAAATGAGGGCTTATCGGCATTCCTTTCTCGTTGGCAGAA
AATGGATAATTATCTTAATAGGCCAGTGAAATTAATTATTGGTAATCAAGAGATTTTTGGTATTGCTCGCGGAATCGATC
AGCAAGGCGCATTATTATTAGAACAAGATGGCAATATAAAGCCTTATATTGGCGGTGAGATATCCCTTCGAGGTGTCTGA

Upstream 100 bases:

>100_bases
ATATCCGCCAGCAGGTCGCACAGAGATTTTCAATCTGGCTAGAACCAGAGGTACGCTTTATTGCTGATAATGGTGAAGTT
AATGCTGTGGAGCATTTATC

Downstream 100 bases:

>100_bases
TTAATTAATGGGAGATAACTATCTCCCGTATAGATTATTTTCTTAAGCGTACACTTTCTACTGCATGATTGGCACTTTTC
GTCATTATTAAACTTGCTCT

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKDIRVPLRLVSILSDGFFHSGEQLGETLGMSRAAINKHMQTIRDWGLDVFTVPGKGYSLPTSIQLLDEQKILSYLPTGH
VTVLPVVDSTNQYLLDRITELKSGDACVAEYQQAGRGRRGRQWVSPFGANLYLSMYWRLEQGPAAAMGLSLVVGIVMAEV
LQNLGAEKVRVKWPNDLYLNDKKLAGILVELTGKTGDAAQLVIGAGINLTMRGSETNAINQDWINLQDAGVTIDRNKLTA
EILSELRLAVVKFENEGLSAFLSRWQKMDNYLNRPVKLIIGNQEIFGIARGIDQQGALLLEQDGNIKPYIGGEISLRGV

Sequences:

>Translated_319_residues
MKDIRVPLRLVSILSDGFFHSGEQLGETLGMSRAAINKHMQTIRDWGLDVFTVPGKGYSLPTSIQLLDEQKILSYLPTGH
VTVLPVVDSTNQYLLDRITELKSGDACVAEYQQAGRGRRGRQWVSPFGANLYLSMYWRLEQGPAAAMGLSLVVGIVMAEV
LQNLGAEKVRVKWPNDLYLNDKKLAGILVELTGKTGDAAQLVIGAGINLTMRGSETNAINQDWINLQDAGVTIDRNKLTA
EILSELRLAVVKFENEGLSAFLSRWQKMDNYLNRPVKLIIGNQEIFGIARGIDQQGALLLEQDGNIKPYIGGEISLRGV
>Mature_319_residues
MKDIRVPLRLVSILSDGFFHSGEQLGETLGMSRAAINKHMQTIRDWGLDVFTVPGKGYSLPTSIQLLDEQKILSYLPTGH
VTVLPVVDSTNQYLLDRITELKSGDACVAEYQQAGRGRRGRQWVSPFGANLYLSMYWRLEQGPAAAMGLSLVVGIVMAEV
LQNLGAEKVRVKWPNDLYLNDKKLAGILVELTGKTGDAAQLVIGAGINLTMRGSETNAINQDWINLQDAGVTIDRNKLTA
EILSELRLAVVKFENEGLSAFLSRWQKMDNYLNRPVKLIIGNQEIFGIARGIDQQGALLLEQDGNIKPYIGGEISLRGV

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=317, Percent_Identity=75.7097791798107, Blast_Score=504, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24644246, Length=274, Percent_Identity=26.6423357664234, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35165; Mature: 35165

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIRVPLRLVSILSDGFFHSGEQLGETLGMSRAAINKHMQTIRDWGLDVFTVPGKGYSL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
PTSIQLLDEQKILSYLPTGHVTVLPVVDSTNQYLLDRITELKSGDACVAEYQQAGRGRRG
CCHHEEHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
RQWVSPFGANLYLSMYWRLEQGPAAAMGLSLVVGIVMAEVLQNLGAEKVRVKWPNDLYLN
CHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEC
DKKLAGILVELTGKTGDAAQLVIGAGINLTMRGSETNAINQDWINLQDAGVTIDRNKLTA
CHHEEEEEEEEECCCCCCEEEEEECCCEEEEECCCCCCCCCCCCEEECCCEEEECHHHHH
EILSELRLAVVKFENEGLSAFLSRWQKMDNYLNRPVKLIIGNQEIFGIARGIDQQGALLL
HHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCEEE
EQDGNIKPYIGGEISLRGV
ECCCCCCCEECCEEEEECC
>Mature Secondary Structure
MKDIRVPLRLVSILSDGFFHSGEQLGETLGMSRAAINKHMQTIRDWGLDVFTVPGKGYSL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
PTSIQLLDEQKILSYLPTGHVTVLPVVDSTNQYLLDRITELKSGDACVAEYQQAGRGRRG
CCHHEEHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
RQWVSPFGANLYLSMYWRLEQGPAAAMGLSLVVGIVMAEVLQNLGAEKVRVKWPNDLYLN
CHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEC
DKKLAGILVELTGKTGDAAQLVIGAGINLTMRGSETNAINQDWINLQDAGVTIDRNKLTA
CHHEEEEEEEEECCCCCCEEEEEECCCEEEEECCCCCCCCCCCCEEECCCEEEECHHHHH
EILSELRLAVVKFENEGLSAFLSRWQKMDNYLNRPVKLIIGNQEIFGIARGIDQQGALLL
HHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCEEE
EQDGNIKPYIGGEISLRGV
ECCCCCCCEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]