Click here to switch to the map view.

The map label for this gene is vraS [H]

Identifier: 169834224

GI number: 169834224

Start: 448353

End: 449348

Strand: Direct

Name: vraS [H]

Synonym: SPH_0493

Alternate gene names: 169834224

Gene position: 448353-449348 (Clockwise)

Preceding gene: 169833637

Following gene: 169833867

Centisome position: 19.97

GC content: 43.57

Gene sequence:

>996_bases
ATGAAAAAACAAGCCTATGTCATTATTGCTCTCACCTCCTTCCTGTTTGTCTTTTTTTTCTCCCACAGCTTGCTGGAAAT
ACTTGATTTTGACTGGTCTATCTTTTTGCACGATGTCGAAAAAACAGAAAAATTTGTCTTTTTATTGTTGGTTTTCAGCA
TGTCCATAACCTGTCTCTTAGCCCTGTTTTGGCGAGGGATCGAAGAGCTTTCTCTAAGAAAAATGCAGGCTAATCTCAAG
CGTTTATTGGCAGGGCAAGAAGTGGTTCAGGTTGCAGATCCAGATTTGGATGCCAGTTTCAAGTCCTTATCAGGTAAACT
TAACCTTTTGACAGAAGCTCTTCAAAAAGCTGAAAATCATAGCCTTGCTCAGGAAGAGGAAATCATCGAGAAAGAACGGA
AGCGAATTGCTCGGGATTTGCACGATACAGTCAGTCAGGAGTTGTTTGCGGCCCACATGATTTTATCGGGTATCAGTCAG
CAGGCTTTGAAATTGGATAGAGAAAAGATGCAGATCCAGTTGCAGAGTGTCACAGCTATTTTAGAAACTGCCCAGAAGGA
TTTGCGGGTTTTGCTCTTGCATTTGCGACCAGTTGAACTGGAGCAGAAGAGCTTGATAGAAGGGATTCAGATTCTCTTAA
AAGAGCTTGAGGACAAGAGTGATCTTAGGGTTAGTCTCAAGCAGAATATGACGAAATTGCCTAAGAAAATCGAGGAGCAT
ATCTTCCGTATCCTGCAAGAGTTGATTAGCAATACCCTCCGCCATGCCCAGGCATCTTGCCTAGATGTCTACCTCTATCA
GACAGATGTTGAATTGCAACTGAAGGTGGTGGACAATGGGATTGGTTTCCAGTTAGGGAGCTTAGACGACTTGAGTTATG
GACTGCGAAATATCAAGGAGCGGGTTGAAGATATGGCAGGGACGGTTCAACTCTTGACAGCTCCCAAGCAAGGGCTGGCG
GTTGATATCCGTATTCCCCTGTTAGATAAGGAATGA

Upstream 100 bases:

>100_bases
ATGAACACTATCATCAAGAAACAAAAGACTATCTCAAGAGTAACAAGAGTGTCAAGATTTTCTTGACCACTATGATTGGT
GATGTGGAGGTGGTTAGAGG

Downstream 100 bases:

>100_bases
TAAAGGAGTAAAGATGAAAATTTTACTAGTAGATGACCATGAAATGGTCCGTTTGGGTTTGAAAAGCTACTTTGACCTCC
AAGACGATGTAGAAGTTGTG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEKFVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLK
RLLAGQEVVQVADPDLDASFKSLSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELEDKSDLRVSLKQNMTKLPKKIEEH
IFRILQELISNTLRHAQASCLDVYLYQTDVELQLKVVDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLA
VDIRIPLLDKE

Sequences:

>Translated_331_residues
MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEKFVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLK
RLLAGQEVVQVADPDLDASFKSLSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELEDKSDLRVSLKQNMTKLPKKIEEH
IFRILQELISNTLRHAQASCLDVYLYQTDVELQLKVVDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLA
VDIRIPLLDKE
>Mature_331_residues
MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEKFVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLK
RLLAGQEVVQVADPDLDASFKSLSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELEDKSDLRVSLKQNMTKLPKKIEEH
IFRILQELISNTLRHAQASCLDVYLYQTDVELQLKVVDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLA
VDIRIPLLDKE

Specific function: Member of the two-component regulatory system vraS/vraR involved in the control of the cell wall peptidoglycan biosynthesis. Probably activates vraR by phosphorylation [H]

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=196, Percent_Identity=27.5510204081633, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788812, Length=196, Percent_Identity=27.0408163265306, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082311, Length=247, Percent_Identity=27.1255060728745, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011712
- InterPro:   IPR017202 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 37906; Mature: 37906

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEKFVFLLLVFSMSITCLL
CCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKSLSGKLNLLTEALQKAENH
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHCC
SLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQQALKLDREKMQIQLQSVTAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELEDKSDLRVSLKQNMTKLPKKIEEH
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IFRILQELISNTLRHAQASCLDVYLYQTDVELQLKVVDNGIGFQLGSLDDLSYGLRNIKE
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
RVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE
HHHHHHHHHHHEECCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEKFVFLLLVFSMSITCLL
CCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKSLSGKLNLLTEALQKAENH
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHCC
SLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQQALKLDREKMQIQLQSVTAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELEDKSDLRVSLKQNMTKLPKKIEEH
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IFRILQELISNTLRHAQASCLDVYLYQTDVELQLKVVDNGIGFQLGSLDDLSYGLRNIKE
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
RVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE
HHHHHHHHHHHEECCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA