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The map label for this gene is liaR [H]

Identifier: 169833867

GI number: 169833867

Start: 449362

End: 449994

Strand: Direct

Name: liaR [H]

Synonym: SPH_0494

Alternate gene names: 169833867

Gene position: 449362-449994 (Clockwise)

Preceding gene: 169834224

Following gene: 169834068

Centisome position: 20.01

GC content: 46.76

Gene sequence:

>633_bases
ATGAAAATTTTACTAGTAGATGACCATGAAATGGTCCGTTTGGGTTTGAAAAGCTACTTTGACCTCCAAGACGATGTAGA
AGTTGTGGGTGAGGCGTCCAACGGGTCTCAAGGGATTGACTTGGCCTTGGAACTGCGTCCAGATGTCATTGTCATGGATA
TTGTCATGCCTGAGATGAATGGGATTGACGCGACCTTAGCAATCCTTAAAGAATGGCCTGAAGCCAAGATTTTGATTGTG
ACCTCTTATTTGGACAATGAAAAAATCATGCCAGTCTTAGATGCTGGTGCCAAAGGCTATATGCTCAAGACTTCTAGTGC
AGATGAATTGCTTCATGCCGTCAGTAAGGTAGCTGCTGGCGAGCTGGCCATTGAGCAAGAGGTTAGCAAGAAGGTTGAAT
ACCACCGCAATCATATGGAACTTCATGAAGAATTGACTGCGCGTGAGCGAGATGTTCTCCAACTCATCGCCAAGGGCTAC
GAAAATCAGCGCATCGCAGATGACCTCTTCATCTCTCTCAAGACGGTCAAGACCCACGTGTCCAACATTCTTGCCAAACT
TGAAGTTAGCGATCGTACCCAGGCGGCTGTCTATGCCTTTCAGCACCATTTGGTCGGGCATGAGGAGTTTTAG

Upstream 100 bases:

>100_bases
GATATGGCAGGGACGGTTCAACTCTTGACAGCTCCCAAGCAAGGGCTGGCGGTTGATATCCGTATTCCCCTGTTAGATAA
GGAATGATAAAGGAGTAAAG

Downstream 100 bases:

>100_bases
ATGAGTTTAGCAGATTTACTTGAGGAGCTAGAAGCAGCAAAAGACTCTAAAAAAGCAAGGTCCATGGAAGCCTATATGCG
CCATCAATTTTCTTTTCTAG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALELRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIV
TSYLDNEKIMPVLDAGAKGYMLKTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF

Sequences:

>Translated_210_residues
MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALELRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIV
TSYLDNEKIMPVLDAGAKGYMLKTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF
>Mature_210_residues
MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALELRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIV
TSYLDNEKIMPVLDAGAKGYMLKTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=36.3636363636364, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787473, Length=208, Percent_Identity=40.8653846153846, Blast_Score=120, Evalue=5e-29,
Organism=Escherichia coli, GI1788222, Length=214, Percent_Identity=29.4392523364486, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1790102, Length=201, Percent_Identity=31.8407960199005, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788712, Length=207, Percent_Identity=25.6038647342995, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786747, Length=206, Percent_Identity=25.7281553398058, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788546, Length=194, Percent_Identity=26.2886597938144, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082052, Length=103, Percent_Identity=33.9805825242718, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=35.7798165137615, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23562; Mature: 23562

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALELRPDVIVMDIVMPEMN
CEEEEECCHHHHHHHHHHHCCCCCCHHHEECCCCCCCCCEEEEEECCCEEEEEEECCCCC
GIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYMLKTSSADELLHAVSKVAAG
CCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHHH
ELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGYENQRIADDLFISLKTVKTHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
SNILAKLEVSDRTQAAVYAFQHHLVGHEEF
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALELRPDVIVMDIVMPEMN
CEEEEECCHHHHHHHHHHHCCCCCCHHHEECCCCCCCCCEEEEEECCCEEEEEEECCCCC
GIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYMLKTSSADELLHAVSKVAAG
CCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHHH
ELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGYENQRIADDLFISLKTVKTHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
SNILAKLEVSDRTQAAVYAFQHHLVGHEEF
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]