Click here to switch to the map view.

The map label for this gene is thiI

Identifier: 169824256

GI number: 169824256

Start: 622104

End: 623279

Strand: Direct

Name: thiI

Synonym: FMG_0559

Alternate gene names: 169824256

Gene position: 622104-623279 (Clockwise)

Preceding gene: 169824255

Following gene: 169824257

Centisome position: 34.61

GC content: 32.65

Gene sequence:

>1176_bases
ATGTTAGATTGGTATATATCTGTTAGTTTTGGTGAACTAACATTAAAAGGAAAGAACAGACACACTTTTGAAAAGCGTGC
GATAAGTAAAATATTGGATGCGATTTCTGATTATAAAATCGAAGAATATTATCAAGAGCAAGGAAAATTATACATCAAAG
CCGATGTAAATGATTTTGATGAAATTATAGATCATATTAAGAAAGTTTTTGGAATAGTGTACATTAGTCCTTGCGTTAAA
TGTGAAAAAACGGTTGAAAGTATGCAAGACGGGGTATTAAAGATAATAGAAGGTAAAATTATCAAAGATAAACTTCAAAC
TTTCAAAGTTGATGTTCATAGAGTTGACAAGAGATTCGAACCTAAATCGCCTGAGTTAAATCCACTTTTAGGTGGCACTA
TATTAAAAAAATATGGAAATTACGTTAAGGTTGACATCAAGAATCCTGATTTTTTCATTTATGTAGACATTAAGGATAAT
TGTTATGTTTACACTGATAGAATAAAAGGATGGGGAGGACTTCCAATTGGAAGTTCCGGAAGAGGTTTGTTGTTATTATC
TGGTGGTATAGACAGTCCGGTAGCTGCTTTTTTGATGGCGAAAAGAGGAGTGCGTGTAGATTGTTTGCATTTCCACAGTT
ATCCATTCACAAGTCAAAGAGGATTTGAGAAGGTTAAGCAGTTAGCAGAAGAAGTTTCATATTACACTGGCAATATTAAC
TTTTATTCTGTGAATTTGCTTCCTGTTTACAAAGCTATCACTAAAAACTGCAAAGAAAGAATGATGACTATTATTTCTAG
AAGATTCATGATGAGAATTGCAGAAAGAATTGCGAATGAAAACAAAATAGACGCTTTGATTACAGGAGAAAGTTTGGGAC
AAGTTGCAAGCCAAACTATTCAAGGTGTTTCAGTTATTAATGAAGTTACAAGTCTTCCTATTTTAAGGCCTTTAATTGCA
AGTGATAAAACAGAAATCATAGAAATTGCAAGGGAAATTGGAACGTATGAAACATCGATTTTGCCTTTTGAAGATTGTTG
CACGGTGTTTACACCGAAAAGACCAGTCACTAAACCAAGAATTTACGATGTCAAAAGAGAAGAAGAAAATTTAGATATTG
AAGCCTTGGTTCAAGAGTGTATTGATAATATGGAATTGATTAAGATTAGACAATAG

Upstream 100 bases:

>100_bases
AATTTCATTTACAGATGACACACCTGAAGATGTTTTGGATGTATTTGTTGAGAAATTAAAGCTTAGATTAGAAGATATTA
GAGATATTATGAGGAGATAA

Downstream 100 bases:

>100_bases
GAGGAAATATGAAAAAAGCAGGAGTTATTATAGGTATTATTGTAGTATTAGCATTATTGTTAATACCACAATACAATGGA
TTAGTTCAGTCAAAAGAAAA

Product: thiamine biosynthesis protein

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MLDWYISVSFGELTLKGKNRHTFEKRAISKILDAISDYKIEEYYQEQGKLYIKADVNDFDEIIDHIKKVFGIVYISPCVK
CEKTVESMQDGVLKIIEGKIIKDKLQTFKVDVHRVDKRFEPKSPELNPLLGGTILKKYGNYVKVDIKNPDFFIYVDIKDN
CYVYTDRIKGWGGLPIGSSGRGLLLLSGGIDSPVAAFLMAKRGVRVDCLHFHSYPFTSQRGFEKVKQLAEEVSYYTGNIN
FYSVNLLPVYKAITKNCKERMMTIISRRFMMRIAERIANENKIDALITGESLGQVASQTIQGVSVINEVTSLPILRPLIA
SDKTEIIEIAREIGTYETSILPFEDCCTVFTPKRPVTKPRIYDVKREEENLDIEALVQECIDNMELIKIRQ

Sequences:

>Translated_391_residues
MLDWYISVSFGELTLKGKNRHTFEKRAISKILDAISDYKIEEYYQEQGKLYIKADVNDFDEIIDHIKKVFGIVYISPCVK
CEKTVESMQDGVLKIIEGKIIKDKLQTFKVDVHRVDKRFEPKSPELNPLLGGTILKKYGNYVKVDIKNPDFFIYVDIKDN
CYVYTDRIKGWGGLPIGSSGRGLLLLSGGIDSPVAAFLMAKRGVRVDCLHFHSYPFTSQRGFEKVKQLAEEVSYYTGNIN
FYSVNLLPVYKAITKNCKERMMTIISRRFMMRIAERIANENKIDALITGESLGQVASQTIQGVSVINEVTSLPILRPLIA
SDKTEIIEIAREIGTYETSILPFEDCCTVFTPKRPVTKPRIYDVKREEENLDIEALVQECIDNMELIKIRQ
>Mature_391_residues
MLDWYISVSFGELTLKGKNRHTFEKRAISKILDAISDYKIEEYYQEQGKLYIKADVNDFDEIIDHIKKVFGIVYISPCVK
CEKTVESMQDGVLKIIEGKIIKDKLQTFKVDVHRVDKRFEPKSPELNPLLGGTILKKYGNYVKVDIKNPDFFIYVDIKDN
CYVYTDRIKGWGGLPIGSSGRGLLLLSGGIDSPVAAFLMAKRGVRVDCLHFHSYPFTSQRGFEKVKQLAEEVSYYTGNIN
FYSVNLLPVYKAITKNCKERMMTIISRRFMMRIAERIANENKIDALITGESLGQVASQTIQGVSVINEVTSLPILRPLIA
SDKTEIIEIAREIGTYETSILPFEDCCTVFTPKRPVTKPRIYDVKREEENLDIEALVQECIDNMELIKIRQ

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=293, Percent_Identity=32.0819112627986, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_FINM2 (B0RZV0)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001691867.1
- ProteinModelPortal:   B0RZV0
- SMR:   B0RZV0
- GeneID:   6019269
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0559
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   CLSK2541985
- BioCyc:   FMAG334413:FMG_0559-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 44716; Mature: 44716

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS51165 THUMP

Important sites: BINDING 267-267 BINDING 289-289 BINDING 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDWYISVSFGELTLKGKNRHTFEKRAISKILDAISDYKIEEYYQEQGKLYIKADVNDFD
CCEEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHH
EIIDHIKKVFGIVYISPCVKCEKTVESMQDGVLKIIEGKIIKDKLQTFKVDVHRVDKRFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
PKSPELNPLLGGTILKKYGNYVKVDIKNPDFFIYVDIKDNCYVYTDRIKGWGGLPIGSSG
CCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCC
RGLLLLSGGIDSPVAAFLMAKRGVRVDCLHFHSYPFTSQRGFEKVKQLAEEVSYYTGNIN
CEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEECCEE
FYSVNLLPVYKAITKNCKERMMTIISRRFMMRIAERIANENKIDALITGESLGQVASQTI
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHH
QGVSVINEVTSLPILRPLIASDKTEIIEIAREIGTYETSILPFEDCCTVFTPKRPVTKPR
HHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCC
IYDVKREEENLDIEALVQECIDNMELIKIRQ
EEECCCCCCCCCHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MLDWYISVSFGELTLKGKNRHTFEKRAISKILDAISDYKIEEYYQEQGKLYIKADVNDFD
CCEEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHH
EIIDHIKKVFGIVYISPCVKCEKTVESMQDGVLKIIEGKIIKDKLQTFKVDVHRVDKRFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
PKSPELNPLLGGTILKKYGNYVKVDIKNPDFFIYVDIKDNCYVYTDRIKGWGGLPIGSSG
CCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCC
RGLLLLSGGIDSPVAAFLMAKRGVRVDCLHFHSYPFTSQRGFEKVKQLAEEVSYYTGNIN
CEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEECCEE
FYSVNLLPVYKAITKNCKERMMTIISRRFMMRIAERIANENKIDALITGESLGQVASQTI
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHH
QGVSVINEVTSLPILRPLIASDKTEIIEIAREIGTYETSILPFEDCCTVFTPKRPVTKPR
HHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCC
IYDVKREEENLDIEALVQECIDNMELIKIRQ
EEECCCCCCCCCHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA