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The map label for this gene is iscS2 [H]

Identifier: 169824255

GI number: 169824255

Start: 620976

End: 622103

Strand: Direct

Name: iscS2 [H]

Synonym: FMG_0558

Alternate gene names: 169824255

Gene position: 620976-622103 (Clockwise)

Preceding gene: 169824251

Following gene: 169824256

Centisome position: 34.55

GC content: 30.67

Gene sequence:

>1128_bases
ATGATATATTTAGATAATGCTGCAACTACGAAAGTATCTGAAAATGCAGTAAAAGAAATGGTTAATGCAATGACTGTTGA
TTTTGGTAATCCTTCCAGCTTGTACGATTTTGGAATGAAGGCTGAAAAACTTGTTACGGATTCAAGAAATTCAATTGCAA
ACTTATTAAATGTTAAGCCAGAAAATGTGTTTTTTACATCTTGTGGGACTGAAGGTAATAACATTTCAATAAACGGAAGT
GTTACAAATGATAAAAAAGAAGAATTTATAACTACAAGTATTGAACATTCTTCTGTGTTTAAGACTTATAATAAATTGAA
GGAAAATAGAACTGTTCATTTTGTAAATGTAGGTGATGATTTTAAAGTTGATGATATTATTGATTTGATAAATGACAAGA
CTCGTTTAGTTTCAGTTATGCATGTCAATAATGAAACAGGAAAAATTCTACCAATTACAGAATTGGGAAAGAAAATCAAA
GAAAAAAATCCTAATACTTTGTTCCACGTAGATGGTATTCAAGGTTTTGGCAAAATTAAATTGAATCTAAATGAAGCTAA
GGTTGATTTCTACACAATTAGCGGTCACAAGATTCACGCTCCAAAAGGAATTGGTGCGATTTTCATTAGAAATCCTGAAA
GAGTAAAGCCTATTATAACAGGAGGATCACAAGAATTTGGGATTTCACCAGGAACAGAAAATGTTGCAGGGATTGTGGCT
TTGAAATGCGCGAGTGAAGAAGTTTTTTCAAAAATTGATGAAACTTACGACAGATTATACAAGATGAAATCATATTTGAT
GGATGAATTATCCAATATTGATGGTGTGTATTTTAATTCCAAATTGGAAGGATACAGTCCATACATTACAAATGTGTGTT
TTAATTACATTAAGAGCGAAGTGTTGCTACACTATTTGGAACAAGATGGAATTTATATTTCTACAGGAAGTGCATGTAAT
GGTTCCAAAAAATCAAGAATTATTCAAAGCATCAATACACCAGATGAATTTTCAGATGGATGTATAAGAATTTCATTTAC
AGATGACACACCTGAAGATGTTTTGGATGTATTTGTTGAGAAATTAAAGCTTAGATTAGAAGATATTAGAGATATTATGA
GGAGATAA

Upstream 100 bases:

>100_bases
TCATTCATTTTATCACCATTTCCATTATACTATGATTTTGGTAAATTTTCTCTACATGTTATAATATTAATATGATTTAA
AATAGGAATGAAGGGATATA

Downstream 100 bases:

>100_bases
ATGTTAGATTGGTATATATCTGTTAGTTTTGGTGAACTAACATTAAAAGGAAAGAACAGACACACTTTTGAAAAGCGTGC
GATAAGTAAAATATTGGATG

Product: iron-sulfur cofactor synthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MIYLDNAATTKVSENAVKEMVNAMTVDFGNPSSLYDFGMKAEKLVTDSRNSIANLLNVKPENVFFTSCGTEGNNISINGS
VTNDKKEEFITTSIEHSSVFKTYNKLKENRTVHFVNVGDDFKVDDIIDLINDKTRLVSVMHVNNETGKILPITELGKKIK
EKNPNTLFHVDGIQGFGKIKLNLNEAKVDFYTISGHKIHAPKGIGAIFIRNPERVKPIITGGSQEFGISPGTENVAGIVA
LKCASEEVFSKIDETYDRLYKMKSYLMDELSNIDGVYFNSKLEGYSPYITNVCFNYIKSEVLLHYLEQDGIYISTGSACN
GSKKSRIIQSINTPDEFSDGCIRISFTDDTPEDVLDVFVEKLKLRLEDIRDIMRR

Sequences:

>Translated_375_residues
MIYLDNAATTKVSENAVKEMVNAMTVDFGNPSSLYDFGMKAEKLVTDSRNSIANLLNVKPENVFFTSCGTEGNNISINGS
VTNDKKEEFITTSIEHSSVFKTYNKLKENRTVHFVNVGDDFKVDDIIDLINDKTRLVSVMHVNNETGKILPITELGKKIK
EKNPNTLFHVDGIQGFGKIKLNLNEAKVDFYTISGHKIHAPKGIGAIFIRNPERVKPIITGGSQEFGISPGTENVAGIVA
LKCASEEVFSKIDETYDRLYKMKSYLMDELSNIDGVYFNSKLEGYSPYITNVCFNYIKSEVLLHYLEQDGIYISTGSACN
GSKKSRIIQSINTPDEFSDGCIRISFTDDTPEDVLDVFVEKLKLRLEDIRDIMRR
>Mature_375_residues
MIYLDNAATTKVSENAVKEMVNAMTVDFGNPSSLYDFGMKAEKLVTDSRNSIANLLNVKPENVFFTSCGTEGNNISINGS
VTNDKKEEFITTSIEHSSVFKTYNKLKENRTVHFVNVGDDFKVDDIIDLINDKTRLVSVMHVNNETGKILPITELGKKIK
EKNPNTLFHVDGIQGFGKIKLNLNEAKVDFYTISGHKIHAPKGIGAIFIRNPERVKPIITGGSQEFGISPGTENVAGIVA
LKCASEEVFSKIDETYDRLYKMKSYLMDELSNIDGVYFNSKLEGYSPYITNVCFNYIKSEVLLHYLEQDGIYISTGSACN
GSKKSRIIQSINTPDEFSDGCIRISFTDDTPEDVLDVFVEKLKLRLEDIRDIMRR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=31.4903846153846, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI32307132, Length=386, Percent_Identity=32.3834196891192, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI48994898, Length=391, Percent_Identity=35.0383631713555, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1789175, Length=275, Percent_Identity=24.3636363636364, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787970, Length=348, Percent_Identity=22.1264367816092, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25143064, Length=384, Percent_Identity=31.25, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=32.0121951219512, Blast_Score=127, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=381, Percent_Identity=32.8083989501312, Blast_Score=190, Evalue=4e-49,
Organism=Drosophila melanogaster, GI20129463, Length=384, Percent_Identity=31.7708333333333, Blast_Score=185, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42028; Mature: 42028

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNAATTKVSENAVKEMVNAMTVDFGNPSSLYDFGMKAEKLVTDSRNSIANLLNVKP
CEEECCCCCCHHHHHHHHHHHHHEEECCCCCCHHHHHCCCHHHHHHCCHHHHHHHHCCCC
ENVFFTSCGTEGNNISINGSVTNDKKEEFITTSIEHSSVFKTYNKLKENRTVHFVNVGDD
CCEEEEECCCCCCEEEEECCCCCCCHHHHEEECCCHHHHHHHHHHHHCCCEEEEEECCCC
FKVDDIIDLINDKTRLVSVMHVNNETGKILPITELGKKIKEKNPNTLFHVDGIQGFGKIK
CCHHHHHHHHCCHHHEEEEEEECCCCCCEEEHHHHHHHHHHCCCCEEEEECCCCCCEEEE
LNLNEAKVDFYTISGHKIHAPKGIGAIFIRNPERVKPIITGGSQEFGISPGTENVAGIVA
EEEEEEEEEEEEECCCEEECCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCEEEEE
LKCASEEVFSKIDETYDRLYKMKSYLMDELSNIDGVYFNSKLEGYSPYITNVCFNYIKSE
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHH
VLLHYLEQDGIYISTGSACNGSKKSRIIQSINTPDEFSDGCIRISFTDDTPEDVLDVFVE
HHHHHHHHCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHH
KLKLRLEDIRDIMRR
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYLDNAATTKVSENAVKEMVNAMTVDFGNPSSLYDFGMKAEKLVTDSRNSIANLLNVKP
CEEECCCCCCHHHHHHHHHHHHHEEECCCCCCHHHHHCCCHHHHHHCCHHHHHHHHCCCC
ENVFFTSCGTEGNNISINGSVTNDKKEEFITTSIEHSSVFKTYNKLKENRTVHFVNVGDD
CCEEEEECCCCCCEEEEECCCCCCCHHHHEEECCCHHHHHHHHHHHHCCCEEEEEECCCC
FKVDDIIDLINDKTRLVSVMHVNNETGKILPITELGKKIKEKNPNTLFHVDGIQGFGKIK
CCHHHHHHHHCCHHHEEEEEEECCCCCCEEEHHHHHHHHHHCCCCEEEEECCCCCCEEEE
LNLNEAKVDFYTISGHKIHAPKGIGAIFIRNPERVKPIITGGSQEFGISPGTENVAGIVA
EEEEEEEEEEEEECCCEEECCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCEEEEE
LKCASEEVFSKIDETYDRLYKMKSYLMDELSNIDGVYFNSKLEGYSPYITNVCFNYIKSE
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHH
VLLHYLEQDGIYISTGSACNGSKKSRIIQSINTPDEFSDGCIRISFTDDTPEDVLDVFVE
HHHHHHHHCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHH
KLKLRLEDIRDIMRR
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]