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The map label for this gene is wecB [C]

Identifier: 169824199

GI number: 169824199

Start: 566732

End: 567814

Strand: Direct

Name: wecB [C]

Synonym: FMG_0502

Alternate gene names: 169824199

Gene position: 566732-567814 (Clockwise)

Preceding gene: 169824198

Following gene: 169824200

Centisome position: 31.53

GC content: 33.24

Gene sequence:

>1083_bases
TTGAAAATTGTTACAGTAGTTGGAGCTAGACCACAATTTATAAAAGCGGCTATGGTGTCCAAAGAATTAAGAAAAAATTT
TGATGAAGTAATGATACATACTGGTCAACATTTTGACTATAATATGTCTGAAGTTTTCTTTGAAGAAATGAATATTCCTT
ATCCAGATTATAACTTGGGGATTTCTGGGGGAACTCACGGCGAAATGACTGGTAAAATGTTGATGGAAATCGAAAAAATA
TTGATAAAAGAACAACCAGATATGGTTTTATTATACGGAGACACCAATTCAACATTGGCAGGAGCATTGGCAGCTGTTAA
GCTTCATATTCCGATTTGTCATGTAGAGGCTGGTAATAGATTGGGTACACTTGATAATCCAGAAGAAGTGAACAGAATTA
TGACTGATCATTCTTCTAAAGTTTTATGTTGTTGTACTGAATCGGCGCTTGGATTTTTGAAAAAAGAAGGCTTGGAAGAT
CGAGCAACTTTGGTTGGCGATCCAATGTATGATGCTTTCTTGGAATATTCTAAGAAAGTTTGCGATAAAATTCCTGAAAA
AATAATTGATTTGAATGGAGAAGAAGTAAATCTTCCAGAAAACTTTTATTATTTAACTTGTCACAGAGCAGAGAATACTG
CATCAATTAAGCCTTTGTCAGAAATACTTGCTGCCATGGAAGAATTGGATGAAAAATGCATATATCCAGTGCATCCTAGA
AATCAAAAAATGGTCCAAGAACTTTCTGAAAAGCACCACTATACAAATATTATTTTCTGCAAACCAGTTGGATACATGAT
GAGTGTGTATTTGGTTAATCACGCGAAGAAAATTGTGACTGATTCAGGCGGTTTGCAAAGAGAAGCGTATTTTGCAAGAA
AACAATGTGTGACTATTTTTGATCATATTGTGTGGCCAGAAACTATGACTGAAAAGAGAAATCAATTATCTAAACCAAAC
AAGGAAGAGATTTTAAAATTATTAAATGAAAAAGTTGATTTCGAAAACTACGATTATCCTTTTGGTGATGGTCATTCTAG
CAAAAAAATTATCGAATGCATCGAAAATTATTTGAATAAATAA

Upstream 100 bases:

>100_bases
ACAAATTACATGTGATATAATTAGTTGTTAGAAACAGATTAAATGATTGAATTTAAAGATTTTGTAATAATAAATATAAA
AATAAAAATAGGAGAAAAAA

Downstream 100 bases:

>100_bases
GAGGAAAATTAATGAATATTTTATATATTAACCATTATGCAGGTTCTCCTGAAATGGGAATGGAGTTTAGAACTTATTAT
TTGGCTAGTGAATGGGTTAA

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKIVTVVGARPQFIKAAMVSKELRKNFDEVMIHTGQHFDYNMSEVFFEEMNIPYPDYNLGISGGTHGEMTGKMLMEIEKI
LIKEQPDMVLLYGDTNSTLAGALAAVKLHIPICHVEAGNRLGTLDNPEEVNRIMTDHSSKVLCCCTESALGFLKKEGLED
RATLVGDPMYDAFLEYSKKVCDKIPEKIIDLNGEEVNLPENFYYLTCHRAENTASIKPLSEILAAMEELDEKCIYPVHPR
NQKMVQELSEKHHYTNIIFCKPVGYMMSVYLVNHAKKIVTDSGGLQREAYFARKQCVTIFDHIVWPETMTEKRNQLSKPN
KEEILKLLNEKVDFENYDYPFGDGHSSKKIIECIENYLNK

Sequences:

>Translated_360_residues
MKIVTVVGARPQFIKAAMVSKELRKNFDEVMIHTGQHFDYNMSEVFFEEMNIPYPDYNLGISGGTHGEMTGKMLMEIEKI
LIKEQPDMVLLYGDTNSTLAGALAAVKLHIPICHVEAGNRLGTLDNPEEVNRIMTDHSSKVLCCCTESALGFLKKEGLED
RATLVGDPMYDAFLEYSKKVCDKIPEKIIDLNGEEVNLPENFYYLTCHRAENTASIKPLSEILAAMEELDEKCIYPVHPR
NQKMVQELSEKHHYTNIIFCKPVGYMMSVYLVNHAKKIVTDSGGLQREAYFARKQCVTIFDHIVWPETMTEKRNQLSKPN
KEEILKLLNEKVDFENYDYPFGDGHSSKKIIECIENYLNK
>Mature_360_residues
MKIVTVVGARPQFIKAAMVSKELRKNFDEVMIHTGQHFDYNMSEVFFEEMNIPYPDYNLGISGGTHGEMTGKMLMEIEKI
LIKEQPDMVLLYGDTNSTLAGALAAVKLHIPICHVEAGNRLGTLDNPEEVNRIMTDHSSKVLCCCTESALGFLKKEGLED
RATLVGDPMYDAFLEYSKKVCDKIPEKIIDLNGEEVNLPENFYYLTCHRAENTASIKPLSEILAAMEELDEKCIYPVHPR
NQKMVQELSEKHHYTNIIFCKPVGYMMSVYLVNHAKKIVTDSGGLQREAYFARKQCVTIFDHIVWPETMTEKRNQLSKPN
KEEILKLLNEKVDFENYDYPFGDGHSSKKIIECIENYLNK

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=379, Percent_Identity=32.7176781002639, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 41135; Mature: 41135

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVTVVGARPQFIKAAMVSKELRKNFDEVMIHTGQHFDYNMSEVFFEEMNIPYPDYNLG
CEEEEEECCCHHHHHHHHHHHHHHHCHHHHHEECCCCCCCCHHHHHHHHCCCCCCCCCCC
ISGGTHGEMTGKMLMEIEKILIKEQPDMVLLYGDTNSTLAGALAAVKLHIPICHVEAGNR
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHEEEEEEEEEEECCCC
LGTLDNPEEVNRIMTDHSSKVLCCCTESALGFLKKEGLEDRATLVGDPMYDAFLEYSKKV
CCCCCCHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHH
CDKIPEKIIDLNGEEVNLPENFYYLTCHRAENTASIKPLSEILAAMEELDEKCIYPVHPR
HHHHHHHHHCCCCCEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
NQKMVQELSEKHHYTNIIFCKPVGYMMSVYLVNHAKKIVTDSGGLQREAYFARKQCVTIF
CHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
DHIVWPETMTEKRNQLSKPNKEEILKLLNEKVDFENYDYPFGDGHSSKKIIECIENYLNK
HHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIVTVVGARPQFIKAAMVSKELRKNFDEVMIHTGQHFDYNMSEVFFEEMNIPYPDYNLG
CEEEEEECCCHHHHHHHHHHHHHHHCHHHHHEECCCCCCCCHHHHHHHHCCCCCCCCCCC
ISGGTHGEMTGKMLMEIEKILIKEQPDMVLLYGDTNSTLAGALAAVKLHIPICHVEAGNR
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHEEEEEEEEEEECCCC
LGTLDNPEEVNRIMTDHSSKVLCCCTESALGFLKKEGLEDRATLVGDPMYDAFLEYSKKV
CCCCCCHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHH
CDKIPEKIIDLNGEEVNLPENFYYLTCHRAENTASIKPLSEILAAMEELDEKCIYPVHPR
HHHHHHHHHCCCCCEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
NQKMVQELSEKHHYTNIIFCKPVGYMMSVYLVNHAKKIVTDSGGLQREAYFARKQCVTIF
CHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
DHIVWPETMTEKRNQLSKPNKEEILKLLNEKVDFENYDYPFGDGHSSKKIIECIENYLNK
HHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA