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The map label for this gene is amyS [H]

Identifier: 169824091

GI number: 169824091

Start: 450736

End: 452181

Strand: Direct

Name: amyS [H]

Synonym: FMG_0394

Alternate gene names: 169824091

Gene position: 450736-452181 (Clockwise)

Preceding gene: 169824088

Following gene: 169824092

Centisome position: 25.07

GC content: 33.4

Gene sequence:

>1446_bases
ATGGGAAACGAAATAATGATGCAAGCTTTTGAATGGTACTTGCCTGATGACGGAAATTATTATAAAAATTTGGCAAAAGA
AGCAAAGAATTTAAAGGAAAAAGGTATTGATGCATTGTGGATTGCACCGATGTTTAAAGCAACGGGAACTAATGATGTTG
GCTACGGAGTGTATGATTTGTACGATTTGGGAGAATTTGATCAAAAAGGATCTGTGAGTACAAAATACGGGACTGTAGAA
GAATTAAAAAAATTAATTGAAGTTTTGCACGAAAATGAAATAAAAGTTTATGCAGATGTAATTCTTAATCACAAAGCGGG
AGCTGATTTCTCGGAAACTTTCAAGGCATACGAAGTAGATCCTAACGACAGGACGAAAAGAATTAGTGATGCTTATGATA
TTGAGGGATGGACTGGGTTTGATTTCAAAGGTAGAAATGGAAAATACTCTGATTTTGTGTGGCATTTTCAACATTTCAAT
GGAGTTGATTTTGATAATAAACAAAATAAGAAAGCTATTTTTGAAATCGCTGGAGAGAATAAAGGATTTAGCAATAGCGT
TTCCAATGAAAAAGGAAATTTCGATTATCTAATGTTCGCAGATATAGACCACAAAAATCCTGATGTACGAGATGAACTTT
TTAGATGGGGAGAATGGTTTGTAAATTATCTTAATGTTGACGGTTTCAGATACGATGCGCTCAAACACATTGACGATCAA
TTCATAATTGATTTTACAAAACATATACAAAATGTTGTGAATCGCGATTTTTATTTTTTCGGTGAATATTGGCTACAAGA
TAAGGACAATACTAATCATTATTTGTATGATACGAAATACAATTTGGATTTATTCGATGTGGCACTTCATTTTAATATGT
ATTCTGCATCGAAAAATGGCAATCAGTTTGATATGAGAAAAATTTTTGACAATACTTTGGTTCAAGAACATCCTACTATT
GCAGTTACATTTGTGGATAATCATGATAGCGAACCTGGCCAATCATTGGAATCATTTGTTGAACCATGGTTCAAAAAGAT
TGCATACGGCTTGATTCTTTTAAGAAAGGACGGATATCCTTGTGTATTTTATGGTGATTATTGTAAAATAGGTGGAGAAT
ATAATATCGAAGGACAAAAGGATATTATCGATAACTTGATGTACATCAGAAAACACTATGCTTTTGGAGATCAAACAGAT
TATATGGAAAGTGAAAATTTGATAGGCTGGATTAGACACGGAGATGATTTTCACAATCCAATGGCTGTTGTAATCAGTAC
GGGTGACATCAATACTTTGAGAATGGATGTAGGAAAGGAAAATGCAGGTAAAAAGTATACGGAAATTACTGGGACTAATT
CTAATGAAATTGTAATCGATGATGATGGATTTGGAGATTTTGAAGTAGGAGCTGGAACTTTGAGCTTGTGGGTATGTAAT
GAGTAA

Upstream 100 bases:

>100_bases
AAATATTGCATTTATTCTCCTTATTCTTAACATTTTAATGTTATAATCTTATTAGATTATACAATATAAAGCAATTAGTT
ACAATTAATAGGAGGAACAA

Downstream 100 bases:

>100_bases
ATTTTATGCGGTAAAAATTGGGAGAAATCCTGGAATATACAATAGTTGGGCTGAATGCCAAGAGCAAGTAAATGGTTTTA
AGGGAGCGATTTACAAATCT

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MGNEIMMQAFEWYLPDDGNYYKNLAKEAKNLKEKGIDALWIAPMFKATGTNDVGYGVYDLYDLGEFDQKGSVSTKYGTVE
ELKKLIEVLHENEIKVYADVILNHKAGADFSETFKAYEVDPNDRTKRISDAYDIEGWTGFDFKGRNGKYSDFVWHFQHFN
GVDFDNKQNKKAIFEIAGENKGFSNSVSNEKGNFDYLMFADIDHKNPDVRDELFRWGEWFVNYLNVDGFRYDALKHIDDQ
FIIDFTKHIQNVVNRDFYFFGEYWLQDKDNTNHYLYDTKYNLDLFDVALHFNMYSASKNGNQFDMRKIFDNTLVQEHPTI
AVTFVDNHDSEPGQSLESFVEPWFKKIAYGLILLRKDGYPCVFYGDYCKIGGEYNIEGQKDIIDNLMYIRKHYAFGDQTD
YMESENLIGWIRHGDDFHNPMAVVISTGDINTLRMDVGKENAGKKYTEITGTNSNEIVIDDDGFGDFEVGAGTLSLWVCN
E

Sequences:

>Translated_481_residues
MGNEIMMQAFEWYLPDDGNYYKNLAKEAKNLKEKGIDALWIAPMFKATGTNDVGYGVYDLYDLGEFDQKGSVSTKYGTVE
ELKKLIEVLHENEIKVYADVILNHKAGADFSETFKAYEVDPNDRTKRISDAYDIEGWTGFDFKGRNGKYSDFVWHFQHFN
GVDFDNKQNKKAIFEIAGENKGFSNSVSNEKGNFDYLMFADIDHKNPDVRDELFRWGEWFVNYLNVDGFRYDALKHIDDQ
FIIDFTKHIQNVVNRDFYFFGEYWLQDKDNTNHYLYDTKYNLDLFDVALHFNMYSASKNGNQFDMRKIFDNTLVQEHPTI
AVTFVDNHDSEPGQSLESFVEPWFKKIAYGLILLRKDGYPCVFYGDYCKIGGEYNIEGQKDIIDNLMYIRKHYAFGDQTD
YMESENLIGWIRHGDDFHNPMAVVISTGDINTLRMDVGKENAGKKYTEITGTNSNEIVIDDDGFGDFEVGAGTLSLWVCN
E
>Mature_480_residues
GNEIMMQAFEWYLPDDGNYYKNLAKEAKNLKEKGIDALWIAPMFKATGTNDVGYGVYDLYDLGEFDQKGSVSTKYGTVEE
LKKLIEVLHENEIKVYADVILNHKAGADFSETFKAYEVDPNDRTKRISDAYDIEGWTGFDFKGRNGKYSDFVWHFQHFNG
VDFDNKQNKKAIFEIAGENKGFSNSVSNEKGNFDYLMFADIDHKNPDVRDELFRWGEWFVNYLNVDGFRYDALKHIDDQF
IIDFTKHIQNVVNRDFYFFGEYWLQDKDNTNHYLYDTKYNLDLFDVALHFNMYSASKNGNQFDMRKIFDNTLVQEHPTIA
VTFVDNHDSEPGQSLESFVEPWFKKIAYGLILLRKDGYPCVFYGDYCKIGGEYNIEGQKDIIDNLMYIRKHYAFGDQTDY
MESENLIGWIRHGDDFHNPMAVVISTGDINTLRMDVGKENAGKKYTEITGTNSNEIVIDDDGFGDFEVGAGTLSLWVCNE

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=493, Percent_Identity=38.3367139959432, Blast_Score=355, Evalue=3e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 55786; Mature: 55655

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNEIMMQAFEWYLPDDGNYYKNLAKEAKNLKEKGIDALWIAPMFKATGTNDVGYGVYDL
CCCHHEEHHHHEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCHHHH
YDLGEFDQKGSVSTKYGTVEELKKLIEVLHENEIKVYADVILNHKAGADFSETFKAYEVD
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHCCEEEECC
PNDRTKRISDAYDIEGWTGFDFKGRNGKYSDFVWHFQHFNGVDFDNKQNKKAIFEIAGEN
CCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCC
KGFSNSVSNEKGNFDYLMFADIDHKNPDVRDELFRWGEWFVNYLNVDGFRYDALKHIDDQ
CCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCH
FIIDFTKHIQNVVNRDFYFFGEYWLQDKDNTNHYLYDTKYNLDLFDVALHFNMYSASKNG
HHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEEECCCEEEEEEEEEEEEECCCCC
NQFDMRKIFDNTLVQEHPTIAVTFVDNHDSEPGQSLESFVEPWFKKIAYGLILLRKDGYP
CHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCC
CVFYGDYCKIGGEYNIEGQKDIIDNLMYIRKHYAFGDQTDYMESENLIGWIRHGDDFHNP
EEEECCCEECCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCC
MAVVISTGDINTLRMDVGKENAGKKYTEITGTNSNEIVIDDDGFGDFEVGAGTLSLWVCN
EEEEEECCCCEEEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCEEECCCEEEEEEEC
E
C
>Mature Secondary Structure 
GNEIMMQAFEWYLPDDGNYYKNLAKEAKNLKEKGIDALWIAPMFKATGTNDVGYGVYDL
CCHHEEHHHHEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCHHHH
YDLGEFDQKGSVSTKYGTVEELKKLIEVLHENEIKVYADVILNHKAGADFSETFKAYEVD
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHCCEEEECC
PNDRTKRISDAYDIEGWTGFDFKGRNGKYSDFVWHFQHFNGVDFDNKQNKKAIFEIAGEN
CCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCC
KGFSNSVSNEKGNFDYLMFADIDHKNPDVRDELFRWGEWFVNYLNVDGFRYDALKHIDDQ
CCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCH
FIIDFTKHIQNVVNRDFYFFGEYWLQDKDNTNHYLYDTKYNLDLFDVALHFNMYSASKNG
HHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEEECCCEEEEEEEEEEEEECCCCC
NQFDMRKIFDNTLVQEHPTIAVTFVDNHDSEPGQSLESFVEPWFKKIAYGLILLRKDGYP
CHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCC
CVFYGDYCKIGGEYNIEGQKDIIDNLMYIRKHYAFGDQTDYMESENLIGWIRHGDDFHNP
EEEECCCEECCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCC
MAVVISTGDINTLRMDVGKENAGKKYTEITGTNSNEIVIDDDGFGDFEVGAGTLSLWVCN
EEEEEECCCCEEEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCEEECCCEEEEEEEC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3924897; 3876333; 3009417; 2999073; 11226887 [H]