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The map label for this gene is slt [H]

Identifier: 169824042

GI number: 169824042

Start: 401139

End: 401780

Strand: Direct

Name: slt [H]

Synonym: FMG_0345

Alternate gene names: 169824042

Gene position: 401139-401780 (Clockwise)

Preceding gene: 169824041

Following gene: 169824043

Centisome position: 22.32

GC content: 30.53

Gene sequence:

>642_bases
ATGAAATTTATTAAAAGATTTTTAATCACGATTATTTCAATTATTGTGATTGTGTTTTTAGTTGGATTTGGCGTGAGTGC
TTATTCTACTATGACAAGACCAGTTAAATATGTAGATTTAGTTAATACATACTCAAAAGAATACAACGTTGATCCACTTT
TGGTTATGAGTGTCATTAAAGTTGAATCTAACTTTGATCCAAGTGTAAAATCAAAGGCTGGAGCTTTAGGGCTTATGCAA
TTGATGCCAGACACTGCTGAAAGTATTAACAATATGAGAAATACTCACTTTACAGTAGATGATTTGAAAAAACCTGACAA
AAACATCGAAATGGGTACTGCATATTTATCGTACTTGTTACATCATTTCAAAAACCACGATTTGGCGATTGCTGCTTACA
ACGGTGGAATTGGAAATGTAAAAGAATGGTTATCGAACGAATCTTTTTCGAAAGATGGTCAAACTTTAGACGATATTCCA
TCTTCTGAGACGAAATACTACGTTGTAAAGGTTGAAAACCAATACAATATTTACAAAATTTTCTACGAAGATACTAAGCT
AGAAGAAAATATGCACAAAAGTTTCAAAGTTTGGGCGAAAAACTACATTAAATTAATCAAAAATATTATTAACAAATATT
AA

Upstream 100 bases:

>100_bases
ATGAGAAAAAAATTAGCGAATTTGACGAAGTTATTGTAAACAACACGACTGAATCAGATTTAATACTCAAAGTTGACGAA
CTAATAGATAGGGAAAAATT

Downstream 100 bases:

>100_bases
AAGGCCTTTTCATAAAGGTCTTTATCACGCAGGGGTGGTGGAATGGTAGACACGCTGTCTTGAGGGGGCAGTGGGAGTAA
TCCCGTAAGAGTTCAAGTCT

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKFIKRFLITIISIIVIVFLVGFGVSAYSTMTRPVKYVDLVNTYSKEYNVDPLLVMSVIKVESNFDPSVKSKAGALGLMQ
LMPDTAESINNMRNTHFTVDDLKKPDKNIEMGTAYLSYLLHHFKNHDLAIAAYNGGIGNVKEWLSNESFSKDGQTLDDIP
SSETKYYVVKVENQYNIYKIFYEDTKLEENMHKSFKVWAKNYIKLIKNIINKY

Sequences:

>Translated_213_residues
MKFIKRFLITIISIIVIVFLVGFGVSAYSTMTRPVKYVDLVNTYSKEYNVDPLLVMSVIKVESNFDPSVKSKAGALGLMQ
LMPDTAESINNMRNTHFTVDDLKKPDKNIEMGTAYLSYLLHHFKNHDLAIAAYNGGIGNVKEWLSNESFSKDGQTLDDIP
SSETKYYVVKVENQYNIYKIFYEDTKLEENMHKSFKVWAKNYIKLIKNIINKY
>Mature_213_residues
MKFIKRFLITIISIIVIVFLVGFGVSAYSTMTRPVKYVDLVNTYSKEYNVDPLLVMSVIKVESNFDPSVKSKAGALGLMQ
LMPDTAESINNMRNTHFTVDDLKKPDKNIEMGTAYLSYLLHHFKNHDLAIAAYNGGIGNVKEWLSNESFSKDGQTLDDIP
SSETKYYVVKVENQYNIYKIFYEDTKLEENMHKSFKVWAKNYIKLIKNIINKY

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=155, Percent_Identity=36.1290322580645, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87082191, Length=174, Percent_Identity=31.0344827586207, Blast_Score=70, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 24524; Mature: 24524

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIKRFLITIISIIVIVFLVGFGVSAYSTMTRPVKYVDLVNTYSKEYNVDPLLVMSVIK
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHH
VESNFDPSVKSKAGALGLMQLMPDTAESINNMRNTHFTVDDLKKPDKNIEMGTAYLSYLL
HCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEHHHHCCCCCCCHHHHHHHHHHH
HHFKNHDLAIAAYNGGIGNVKEWLSNESFSKDGQTLDDIPSSETKYYVVKVENQYNIYKI
HHHCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCHHCCCCCCCEEEEEEECCCCEEEEE
FYEDTKLEENMHKSFKVWAKNYIKLIKNIINKY
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFIKRFLITIISIIVIVFLVGFGVSAYSTMTRPVKYVDLVNTYSKEYNVDPLLVMSVIK
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHH
VESNFDPSVKSKAGALGLMQLMPDTAESINNMRNTHFTVDDLKKPDKNIEMGTAYLSYLL
HCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEHHHHCCCCCCCHHHHHHHHHHH
HHFKNHDLAIAAYNGGIGNVKEWLSNESFSKDGQTLDDIPSSETKYYVVKVENQYNIYKI
HHHCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCHHCCCCCCCEEEEEEECCCCEEEEE
FYEDTKLEENMHKSFKVWAKNYIKLIKNIINKY
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]