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The map label for this gene is polA [H]

Identifier: 169824040

GI number: 169824040

Start: 397920

End: 400547

Strand: Direct

Name: polA [H]

Synonym: FMG_0343

Alternate gene names: 169824040

Gene position: 397920-400547 (Clockwise)

Preceding gene: 169824039

Following gene: 169824041

Centisome position: 22.14

GC content: 34.4

Gene sequence:

>2628_bases
ATGTCAAAAAAAGCTTTAATTATTGACGGCTCATCTCTTTTATTCAGAGGTTTTTATGCTATCAGAGATTTGACAACAAA
AGATGGGATTCACACTAATGGTGTGTTTGGATTTATGAATATGTATTATTCTGTTATGGGAAAAATCAATCCAGATCTTG
TAGTTGTGTGTTTTGATAGAAAAGAACCAACTTTCAGGCACGAAAAATATTCTGAATACAAGGGCAATCGTCAAGAAACT
CCGGAAGAATTAATCGGCCAATTCGATATGTTAAAAGAATTGTTGAAAGCGATGGGAGTTTCATACATTGATTTGAAAGG
ATATGAAGCAGACGACATCGCAGGAACATTGGCAAAACAAGCTTCATCAAAAGATTACGATGTGTATTTGTTGACTGGTG
ACAAGGATTATTTGCAACTTATTGACGACAACACAAAAGTTGTCCTTACAAAAAGAGGAATCACTGAAACAAAAATCTAC
GATGTAGATGTTTTGAAAGAAGAATACGGAATTACTCCTAAACAAATGATTGATTTGAAAGGTTTGATGGGAGATAAATC
GGACAATATTCCAGGAATTGATGGAGTTGGAGAAAAGACTGCACTTAAATACATTCAAAAATACGGAAGTATTGAAGGAT
TGTACGAGCATGTCGATGAAATTTCCGGCAAGAAAACTAAGCAAAAAGTCATTGATGGAGAAGACATTGCATATTTGAGT
AGAGAACTTGGAACGATTAATACAGATGTTCCCGAAGATTTAATGGGATTTGGAATCGAGGATTGTACTATAAAAGAAGC
TGACAATGATCATTTGATTGAAGTCCTTACTAAACTTGAATTCAACAGTTTTATCAAAAAACTTCCAAACGAAGAATCTA
ATGAAAAGTTTGAATATGAAAACTTAGATGAAAAAGTAGATGAAATTAAAGCTTATATTAAAGAGAACAAAAAGTTTACT
TTTGCATTGTTCAATGATGATTATTATGTGGACAATGAGCCTGTGATTTTTGCTATTTACACTGACAAGGTGTATATTAC
AAGAAACGTAGAACTTGTAGAATCATTCAAGGAAGAATTCGAATCGAAAGATATCAACAAGCTTTCATTTGATATCAAAC
CATGTATTTATCACTTAATGCGATATGGTATCGACATTTCGTACAATTACGATGATGTTGTAATCATTGAATATTTGCTC
GATCCATCCAAGACAAGTTATAGCATGGAAAGATCTGACCCGAAAGTTATCGGATATGATTTCAAGCCGCTTGAAGATAT
TACAGGAAAAGGCAAATCCAAGAAGACTATTTTGGAATCTGACGAGAAAGAAATCGACAATTACGTTGTGAAATACATGA
TGTTCACGAACAAATTGTACGAAAAGATTTCAGAAATAGAAGAAGAAGATATGATGCATCTGTACAGAGATGTTGAAATT
CCTTTGATAAAAGTTTTGTGTGACATGGAATTTACGGGAGTTTGTGTCAAGAAAGAAACTTTGGTCGAGATGGGAGAACT
TTTCGAAGATGAGATAAAATCAATCGAAAAAAGTGTTGAAGAACTTATCGGAAAGAAAATCAACATCAACTCATCAAAGC
AATTGGCAGAAGTGTTGTTTGAAGATTTAGAGCTTCCTGTGATAAAGAAGAACAAAACGGGCCCATCGACAGACCAAGAA
GTTTTGGAAGCTTTAAGTGGAAGACACGAAATTATCGACTACATCTTAAGATACCGAACAATTACGAAACTTAAAACAAC
ATATATCGATGGAATGGTGGATTTAATCAAAAAAGACGGCAAAATCCACACGACTTTCCAACAAACTATCGCACAAACTG
GAAGAATTAGCTCAACTAATCCTAACTTGCAAAACATTCCTATCAGAACTGAAGAAGGAAGACTCATTAGAAAAGCATTT
GTTCCAAGCGAAGGAAATATGCTGCTGGATGCGGATTATTCCCAAATAGAGCTTAGAGTGTTGGCTGATTTGGCAAATGA
TGAGGTTATGCTTGATGCATTCAAGCACGGTGCGGATATTCACAGAAAAACTGCCAGCGAAGTGTTCAAGGTTGATTTTG
ATAAAGTAACATCACTTCAAAGATCCAACGCAAAGGCTGTAAACTTCGGTATTGTTTACGGAATTGGAGATTATTCTTTA
AGTAAAGATTTGCATATAACTAGAAAAGAAGCCAAAGAATACATCGAAAATTATTTGGACACTTACAAAGGTATCAAACA
ATACATGGAAGATATAGTTGCAATTGGCAAGGAAAAAGGCTACGTTGAAACTATTATGAATAGAAGAAGATACATTCCAG
AATTGAAATCCAAAAACTACAATGTTAGAAGTTTTGGGGAACGTGTGGCACTTAATACGCCAATCCAAGGATCAGCAGCG
GATATCATCAAAGTTGCTATGGTCAAATTCTACAATAGATTGAACGAAGAAAAACTTAAAGCAAAATTGATTTTGCAGGT
TCACGATGAGCTTATTGTTGATTGTCCAAAAGACGAACGAGAAAAAGTTCTTGAAATAATGAAAGATGTCATGACACATG
CTGTTGATTTGAAAGTCGATATGAAGATTGATGTAAACAGTGCAGATAATTGGTATGATGCAAAATAA

Upstream 100 bases:

>100_bases
ATCATCAAAACTTTCTTGGAAAATGAATTCGAAGCTGGTCGTCACCAAAGAAGAGTTGACAAGATAATGGCTTTCGAAAA
AGAAAACAAGGGATTATAAA

Downstream 100 bases:

>100_bases
AAAAATCATCGTAGTTTGTGGAAATATCGCAAGCGGGAAAAGTACGGTGTGCAGAAGAATTAGAGAAAAAGGCTTTGTTG
TAATAGATTTGGATGAGATT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 875; Mature: 874

Protein sequence:

>875_residues
MSKKALIIDGSSLLFRGFYAIRDLTTKDGIHTNGVFGFMNMYYSVMGKINPDLVVVCFDRKEPTFRHEKYSEYKGNRQET
PEELIGQFDMLKELLKAMGVSYIDLKGYEADDIAGTLAKQASSKDYDVYLLTGDKDYLQLIDDNTKVVLTKRGITETKIY
DVDVLKEEYGITPKQMIDLKGLMGDKSDNIPGIDGVGEKTALKYIQKYGSIEGLYEHVDEISGKKTKQKVIDGEDIAYLS
RELGTINTDVPEDLMGFGIEDCTIKEADNDHLIEVLTKLEFNSFIKKLPNEESNEKFEYENLDEKVDEIKAYIKENKKFT
FALFNDDYYVDNEPVIFAIYTDKVYITRNVELVESFKEEFESKDINKLSFDIKPCIYHLMRYGIDISYNYDDVVIIEYLL
DPSKTSYSMERSDPKVIGYDFKPLEDITGKGKSKKTILESDEKEIDNYVVKYMMFTNKLYEKISEIEEEDMMHLYRDVEI
PLIKVLCDMEFTGVCVKKETLVEMGELFEDEIKSIEKSVEELIGKKININSSKQLAEVLFEDLELPVIKKNKTGPSTDQE
VLEALSGRHEIIDYILRYRTITKLKTTYIDGMVDLIKKDGKIHTTFQQTIAQTGRISSTNPNLQNIPIRTEEGRLIRKAF
VPSEGNMLLDADYSQIELRVLADLANDEVMLDAFKHGADIHRKTASEVFKVDFDKVTSLQRSNAKAVNFGIVYGIGDYSL
SKDLHITRKEAKEYIENYLDTYKGIKQYMEDIVAIGKEKGYVETIMNRRRYIPELKSKNYNVRSFGERVALNTPIQGSAA
DIIKVAMVKFYNRLNEEKLKAKLILQVHDELIVDCPKDEREKVLEIMKDVMTHAVDLKVDMKIDVNSADNWYDAK

Sequences:

>Translated_875_residues
MSKKALIIDGSSLLFRGFYAIRDLTTKDGIHTNGVFGFMNMYYSVMGKINPDLVVVCFDRKEPTFRHEKYSEYKGNRQET
PEELIGQFDMLKELLKAMGVSYIDLKGYEADDIAGTLAKQASSKDYDVYLLTGDKDYLQLIDDNTKVVLTKRGITETKIY
DVDVLKEEYGITPKQMIDLKGLMGDKSDNIPGIDGVGEKTALKYIQKYGSIEGLYEHVDEISGKKTKQKVIDGEDIAYLS
RELGTINTDVPEDLMGFGIEDCTIKEADNDHLIEVLTKLEFNSFIKKLPNEESNEKFEYENLDEKVDEIKAYIKENKKFT
FALFNDDYYVDNEPVIFAIYTDKVYITRNVELVESFKEEFESKDINKLSFDIKPCIYHLMRYGIDISYNYDDVVIIEYLL
DPSKTSYSMERSDPKVIGYDFKPLEDITGKGKSKKTILESDEKEIDNYVVKYMMFTNKLYEKISEIEEEDMMHLYRDVEI
PLIKVLCDMEFTGVCVKKETLVEMGELFEDEIKSIEKSVEELIGKKININSSKQLAEVLFEDLELPVIKKNKTGPSTDQE
VLEALSGRHEIIDYILRYRTITKLKTTYIDGMVDLIKKDGKIHTTFQQTIAQTGRISSTNPNLQNIPIRTEEGRLIRKAF
VPSEGNMLLDADYSQIELRVLADLANDEVMLDAFKHGADIHRKTASEVFKVDFDKVTSLQRSNAKAVNFGIVYGIGDYSL
SKDLHITRKEAKEYIENYLDTYKGIKQYMEDIVAIGKEKGYVETIMNRRRYIPELKSKNYNVRSFGERVALNTPIQGSAA
DIIKVAMVKFYNRLNEEKLKAKLILQVHDELIVDCPKDEREKVLEIMKDVMTHAVDLKVDMKIDVNSADNWYDAK
>Mature_874_residues
SKKALIIDGSSLLFRGFYAIRDLTTKDGIHTNGVFGFMNMYYSVMGKINPDLVVVCFDRKEPTFRHEKYSEYKGNRQETP
EELIGQFDMLKELLKAMGVSYIDLKGYEADDIAGTLAKQASSKDYDVYLLTGDKDYLQLIDDNTKVVLTKRGITETKIYD
VDVLKEEYGITPKQMIDLKGLMGDKSDNIPGIDGVGEKTALKYIQKYGSIEGLYEHVDEISGKKTKQKVIDGEDIAYLSR
ELGTINTDVPEDLMGFGIEDCTIKEADNDHLIEVLTKLEFNSFIKKLPNEESNEKFEYENLDEKVDEIKAYIKENKKFTF
ALFNDDYYVDNEPVIFAIYTDKVYITRNVELVESFKEEFESKDINKLSFDIKPCIYHLMRYGIDISYNYDDVVIIEYLLD
PSKTSYSMERSDPKVIGYDFKPLEDITGKGKSKKTILESDEKEIDNYVVKYMMFTNKLYEKISEIEEEDMMHLYRDVEIP
LIKVLCDMEFTGVCVKKETLVEMGELFEDEIKSIEKSVEELIGKKININSSKQLAEVLFEDLELPVIKKNKTGPSTDQEV
LEALSGRHEIIDYILRYRTITKLKTTYIDGMVDLIKKDGKIHTTFQQTIAQTGRISSTNPNLQNIPIRTEEGRLIRKAFV
PSEGNMLLDADYSQIELRVLADLANDEVMLDAFKHGADIHRKTASEVFKVDFDKVTSLQRSNAKAVNFGIVYGIGDYSLS
KDLHITRKEAKEYIENYLDTYKGIKQYMEDIVAIGKEKGYVETIMNRRRYIPELKSKNYNVRSFGERVALNTPIQGSAAD
IIKVAMVKFYNRLNEEKLKAKLILQVHDELIVDCPKDEREKVLEIMKDVMTHAVDLKVDMKIDVNSADNWYDAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=538, Percent_Identity=30.6691449814126, Blast_Score=240, Evalue=4e-63,
Organism=Homo sapiens, GI139394648, Length=620, Percent_Identity=25.3225806451613, Blast_Score=197, Evalue=4e-50,
Organism=Escherichia coli, GI1790294, Length=929, Percent_Identity=35.1991388589882, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI226510970, Length=205, Percent_Identity=29.7560975609756, Blast_Score=116, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI86563272, Length=373, Percent_Identity=30.5630026809651, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI17933644, Length=516, Percent_Identity=30.2325581395349, Blast_Score=227, Evalue=2e-59,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 100663; Mature: 100532

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKALIIDGSSLLFRGFYAIRDLTTKDGIHTNGVFGFMNMYYSVMGKINPDLVVVCFDR
CCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECC
KEPTFRHEKYSEYKGNRQETPEELIGQFDMLKELLKAMGVSYIDLKGYEADDIAGTLAKQ
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHH
ASSKDYDVYLLTGDKDYLQLIDDNTKVVLTKRGITETKIYDVDVLKEEYGITPKQMIDLK
CCCCCCEEEEEECCHHHHHEECCCCEEEEEECCCCCCEEEEHHHHHHHHCCCHHHHHHHH
GLMGDKSDNIPGIDGVGEKTALKYIQKYGSIEGLYEHVDEISGKKTKQKVIDGEDIAYLS
HHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCHHHHHH
RELGTINTDVPEDLMGFGIEDCTIKEADNDHLIEVLTKLEFNSFIKKLPNEESNEKFEYE
HHHCCCCCCCCHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NLDEKVDEIKAYIKENKKFTFALFNDDYYVDNEPVIFAIYTDKVYITRNVELVESFKEEF
CHHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCEEEEEEECEEEEECCHHHHHHHHHHH
ESKDINKLSFDIKPCIYHLMRYGIDISYNYDDVVIIEYLLDPSKTSYSMERSDPKVIGYD
CCCCCCHHCCCHHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCCCCCCCCCCCEEECC
FKPLEDITGKGKSKKTILESDEKEIDNYVVKYMMFTNKLYEKISEIEEEDMMHLYRDVEI
CCCHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLIKVLCDMEFTGVCVKKETLVEMGELFEDEIKSIEKSVEELIGKKININSSKQLAEVLF
HHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
EDLELPVIKKNKTGPSTDQEVLEALSGRHEIIDYILRYRTITKLKTTYIDGMVDLIKKDG
HHCCCCEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KIHTTFQQTIAQTGRISSTNPNLQNIPIRTEEGRLIRKAFVPSEGNMLLDADYSQIELRV
CEEHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCEEHHHCCCCCCCEEEECCCHHHHHHH
LADLANDEVMLDAFKHGADIHRKTASEVFKVDFDKVTSLQRSNAKAVNFGIVYGIGDYSL
HHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEEECCCCC
SKDLHITRKEAKEYIENYLDTYKGIKQYMEDIVAIGKEKGYVETIMNRRRYIPELKSKNY
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCC
NVRSFGERVALNTPIQGSAADIIKVAMVKFYNRLNEEKLKAKLILQVHDELIVDCPKDER
CHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEECCCHHH
EKVLEIMKDVMTHAVDLKVDMKIDVNSADNWYDAK
HHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SKKALIIDGSSLLFRGFYAIRDLTTKDGIHTNGVFGFMNMYYSVMGKINPDLVVVCFDR
CCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECC
KEPTFRHEKYSEYKGNRQETPEELIGQFDMLKELLKAMGVSYIDLKGYEADDIAGTLAKQ
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHH
ASSKDYDVYLLTGDKDYLQLIDDNTKVVLTKRGITETKIYDVDVLKEEYGITPKQMIDLK
CCCCCCEEEEEECCHHHHHEECCCCEEEEEECCCCCCEEEEHHHHHHHHCCCHHHHHHHH
GLMGDKSDNIPGIDGVGEKTALKYIQKYGSIEGLYEHVDEISGKKTKQKVIDGEDIAYLS
HHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCHHHHHH
RELGTINTDVPEDLMGFGIEDCTIKEADNDHLIEVLTKLEFNSFIKKLPNEESNEKFEYE
HHHCCCCCCCCHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NLDEKVDEIKAYIKENKKFTFALFNDDYYVDNEPVIFAIYTDKVYITRNVELVESFKEEF
CHHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCEEEEEEECEEEEECCHHHHHHHHHHH
ESKDINKLSFDIKPCIYHLMRYGIDISYNYDDVVIIEYLLDPSKTSYSMERSDPKVIGYD
CCCCCCHHCCCHHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCCCCCCCCCCCEEECC
FKPLEDITGKGKSKKTILESDEKEIDNYVVKYMMFTNKLYEKISEIEEEDMMHLYRDVEI
CCCHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLIKVLCDMEFTGVCVKKETLVEMGELFEDEIKSIEKSVEELIGKKININSSKQLAEVLF
HHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
EDLELPVIKKNKTGPSTDQEVLEALSGRHEIIDYILRYRTITKLKTTYIDGMVDLIKKDG
HHCCCCEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KIHTTFQQTIAQTGRISSTNPNLQNIPIRTEEGRLIRKAFVPSEGNMLLDADYSQIELRV
CEEHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCEEHHHCCCCCCCEEEECCCHHHHHHH
LADLANDEVMLDAFKHGADIHRKTASEVFKVDFDKVTSLQRSNAKAVNFGIVYGIGDYSL
HHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEEECCCCC
SKDLHITRKEAKEYIENYLDTYKGIKQYMEDIVAIGKEKGYVETIMNRRRYIPELKSKNY
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCC
NVRSFGERVALNTPIQGSAADIIKVAMVKFYNRLNEEKLKAKLILQVHDELIVDCPKDER
CHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEECCCHHH
EKVLEIMKDVMTHAVDLKVDMKIDVNSADNWYDAK
HHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]