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The map label for this gene is ydaP [H]

Identifier: 169629623

GI number: 169629623

Start: 2583474

End: 2585273

Strand: Reverse

Name: ydaP [H]

Synonym: MAB_2537c

Alternate gene names: 169629623

Gene position: 2585273-2583474 (Counterclockwise)

Preceding gene: 169629624

Following gene: 169629620

Centisome position: 51.02

GC content: 62.17

Gene sequence:

>1800_bases
ATGTCCAGCACGGTCGGCGACATCCTGCTTTCCCGCCTGCGAGAATGGGGTGTCCAACAGGTCTTCGGATATCCGGGCGA
CGGCATCAACGGATTACTGGCGGCCTGGCGTTGCGCCGACAACGACCCACAATTCGTCCAGGCTCGTCACGAGGAGATGG
CCGCCTTCGAAGCAGTGGGTTTCGCGAAGTTCTCCGGCCAACTCGGTGTCTGCACGGCAACATCCGGCCCGGGTGCGATA
CATCTGCTCAACGGCCTCTACGACGCCAAACTGGATCACGTCCCGGTGCTAGCCATTGTCGGCCAGGCCGATCGTTCCGC
AATGGGCGGCTCATACCAGCAAGAGGTCGATCTCTTGAGCCTGTACAAAGACGTCTGCAGTGACTACGTGCAGCAGTGCA
TGGTGCCCCAGCAGTTGCCCAACCTGGTGGATCGAGCGATACGAATAGCGCTGTCCGAGGCCACACCGACGGCCATCATC
GTGCCCTCCGACGTATTCAACCTCGGCTATGAAGCGCCTGTTCATGCCTTCAAGCAGGTTCCGTCAAGCCTTGGCATGTC
GGGATCCCGTTCGATACCCTCTCCCGACGCCCTGAGGGCAGCGGCCGACTTGCTCAACTCGGGCAAGAAGGTAGCCCTGC
TTGTCGGGCAGGGCGCCCGTGGGTGCGTTGCCGAGCTCACCGCCCTTGCCGACCTTTTAGGAGCAGGCGCCGCAAAAGCA
TTGCTGGGCAAGGATGTGCTGCCCGATGATCTTCCCTGGGTCACCGGATCTATCGGTCTGCTCGGCACCACCGCCAGTTG
GCATCTCATGCGCGACTGCGACACCCTGCTCACCGTGGGATCGAACTTTCCCTACACTCAATTCCTTCCGGATCTCGGGC
AGGCCCGCGCGGTGCAGATCGACCGTTCGGGCAAATGGATCGGTATGCGCTACCCGTATGAACTCAACTTGGTTGGCGAC
GCCAAAGACACACTGCAAGCCCTCATCCCCCTTCTGGAGCGCAAGCCCGATCGCTCATGGCGCGAGCACATAGAGAAGAA
GACCCGCGGCTGGTGGACAACCGTGCAACGCACCGCCATGACGAACGCCGATCCGGTCAATCCCATGCGGATTTTCTGGG
AGCTGACGAAACGTATGCCGCCCAATGCCATCATCACCGCGGATTCCGGCTCCTCGGCCAATTGGTACGCGCGTCATCTG
CGCTTCACCGACGGCGTGCGTGGCTCGCTGTCGGGCACGCTCGCGACCATGGGACCTGGTGTGCCGTATGCCATCGGCGC
GAAATGGGCACATCCCGACCGACCTGGAATCGCCTTCGTGGGAGATGGCGCGATGCAGATGAATGGGATGAACGAGCTCA
TCACCATCGCGAGGTACTGGCGGCAGTGGGCCGATCCGCGCCTGGTGGTCGTGGTCCTGCACAACAACGACCTCAACCAG
GTCACCTGGGAGATGCGGGCCATGGAAGGCTCGCCCAAATTCGCTGAATCACAGACTCTTCCGGATGTCGACCATGCGAG
ATTTGCGCGGGGTCTGGACTTGCGCGGCGACAACATCGAGGACCCCGCACAGATCGCCGGCGCATGGGACAGAGCCCTTT
CCGCGGATCGGCCCACCGTACTCGACGTACGCTGCGATCCTGACGTTCCGCCCATTCCCCCGCACGCCACGGTCGACGAG
GTCACATCGCTGGTCGGCGCCGTCGTCGGCGGCGACGAAGACACCGCCGGGTTTACCCGACAAGGCATCAAACAGAAATT
GCAGCAGTATGTTCCGCTCCCAAAGCGGGACCGGCCATAG

Upstream 100 bases:

>100_bases
ACGCCCGATAGGGAATGCCGATCGAGCGTCGAGGCGATCGGAGATCTTGTCATCGCCGCCCGGACGCACGTCACGCACAG
AATCCCTGCGAGGAGGAACC

Downstream 100 bases:

>100_bases
AACCTGCGTCACCGATCAGGCAGAGGCTGATTGGTTTGCATGCGTTCGACGGTCCACATGGACAGCGCCTCACGTGCTCC
CCCACCAACCGGTTCAACCT

Product: putative pyruvate decarboxylase

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MSSTVGDILLSRLREWGVQQVFGYPGDGINGLLAAWRCADNDPQFVQARHEEMAAFEAVGFAKFSGQLGVCTATSGPGAI
HLLNGLYDAKLDHVPVLAIVGQADRSAMGGSYQQEVDLLSLYKDVCSDYVQQCMVPQQLPNLVDRAIRIALSEATPTAII
VPSDVFNLGYEAPVHAFKQVPSSLGMSGSRSIPSPDALRAAADLLNSGKKVALLVGQGARGCVAELTALADLLGAGAAKA
LLGKDVLPDDLPWVTGSIGLLGTTASWHLMRDCDTLLTVGSNFPYTQFLPDLGQARAVQIDRSGKWIGMRYPYELNLVGD
AKDTLQALIPLLERKPDRSWREHIEKKTRGWWTTVQRTAMTNADPVNPMRIFWELTKRMPPNAIITADSGSSANWYARHL
RFTDGVRGSLSGTLATMGPGVPYAIGAKWAHPDRPGIAFVGDGAMQMNGMNELITIARYWRQWADPRLVVVVLHNNDLNQ
VTWEMRAMEGSPKFAESQTLPDVDHARFARGLDLRGDNIEDPAQIAGAWDRALSADRPTVLDVRCDPDVPPIPPHATVDE
VTSLVGAVVGGDEDTAGFTRQGIKQKLQQYVPLPKRDRP

Sequences:

>Translated_599_residues
MSSTVGDILLSRLREWGVQQVFGYPGDGINGLLAAWRCADNDPQFVQARHEEMAAFEAVGFAKFSGQLGVCTATSGPGAI
HLLNGLYDAKLDHVPVLAIVGQADRSAMGGSYQQEVDLLSLYKDVCSDYVQQCMVPQQLPNLVDRAIRIALSEATPTAII
VPSDVFNLGYEAPVHAFKQVPSSLGMSGSRSIPSPDALRAAADLLNSGKKVALLVGQGARGCVAELTALADLLGAGAAKA
LLGKDVLPDDLPWVTGSIGLLGTTASWHLMRDCDTLLTVGSNFPYTQFLPDLGQARAVQIDRSGKWIGMRYPYELNLVGD
AKDTLQALIPLLERKPDRSWREHIEKKTRGWWTTVQRTAMTNADPVNPMRIFWELTKRMPPNAIITADSGSSANWYARHL
RFTDGVRGSLSGTLATMGPGVPYAIGAKWAHPDRPGIAFVGDGAMQMNGMNELITIARYWRQWADPRLVVVVLHNNDLNQ
VTWEMRAMEGSPKFAESQTLPDVDHARFARGLDLRGDNIEDPAQIAGAWDRALSADRPTVLDVRCDPDVPPIPPHATVDE
VTSLVGAVVGGDEDTAGFTRQGIKQKLQQYVPLPKRDRP
>Mature_598_residues
SSTVGDILLSRLREWGVQQVFGYPGDGINGLLAAWRCADNDPQFVQARHEEMAAFEAVGFAKFSGQLGVCTATSGPGAIH
LLNGLYDAKLDHVPVLAIVGQADRSAMGGSYQQEVDLLSLYKDVCSDYVQQCMVPQQLPNLVDRAIRIALSEATPTAIIV
PSDVFNLGYEAPVHAFKQVPSSLGMSGSRSIPSPDALRAAADLLNSGKKVALLVGQGARGCVAELTALADLLGAGAAKAL
LGKDVLPDDLPWVTGSIGLLGTTASWHLMRDCDTLLTVGSNFPYTQFLPDLGQARAVQIDRSGKWIGMRYPYELNLVGDA
KDTLQALIPLLERKPDRSWREHIEKKTRGWWTTVQRTAMTNADPVNPMRIFWELTKRMPPNAIITADSGSSANWYARHLR
FTDGVRGSLSGTLATMGPGVPYAIGAKWAHPDRPGIAFVGDGAMQMNGMNELITIARYWRQWADPRLVVVVLHNNDLNQV
TWEMRAMEGSPKFAESQTLPDVDHARFARGLDLRGDNIEDPAQIAGAWDRALSADRPTVLDVRCDPDVPPIPPHATVDEV
TSLVGAVVGGDEDTAGFTRQGIKQKLQQYVPLPKRDRP

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=587, Percent_Identity=22.1465076660988, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI21361361, Length=568, Percent_Identity=23.943661971831, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1787096, Length=583, Percent_Identity=30.7032590051458, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1790104, Length=573, Percent_Identity=26.7015706806283, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081685, Length=571, Percent_Identity=27.3204903677758, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1786717, Length=557, Percent_Identity=25.4937163375224, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788716, Length=559, Percent_Identity=21.824686940966, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531299, Length=567, Percent_Identity=23.9858906525573, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17531301, Length=569, Percent_Identity=23.9015817223199, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17542570, Length=588, Percent_Identity=24.1496598639456, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323755, Length=572, Percent_Identity=25.3496503496504, Blast_Score=147, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6320816, Length=496, Percent_Identity=24.5967741935484, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19922626, Length=577, Percent_Identity=24.0901213171577, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 65086; Mature: 64955

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTVGDILLSRLREWGVQQVFGYPGDGINGLLAAWRCADNDPQFVQARHEEMAAFEAVG
CCCHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
FAKFSGQLGVCTATSGPGAIHLLNGLYDAKLDHVPVLAIVGQADRSAMGGSYQQEVDLLS
HHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCCHHHHHHHHH
LYKDVCSDYVQQCMVPQQLPNLVDRAIRIALSEATPTAIIVPSDVFNLGYEAPVHAFKQV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCHHHHHHHHH
PSSLGMSGSRSIPSPDALRAAADLLNSGKKVALLVGQGARGCVAELTALADLLGAGAAKA
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHH
LLGKDVLPDDLPWVTGSIGLLGTTASWHLMRDCDTLLTVGSNFPYTQFLPDLGQARAVQI
HHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEE
DRSGKWIGMRYPYELNLVGDAKDTLQALIPLLERKPDRSWREHIEKKTRGWWTTVQRTAM
ECCCCEEEEECCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHC
TNADPVNPMRIFWELTKRMPPNAIITADSGSSANWYARHLRFTDGVRGSLSGTLATMGPG
CCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHEECCCCCCCCCCCCHHHCCCC
VPYAIGAKWAHPDRPGIAFVGDGAMQMNGMNELITIARYWRQWADPRLVVVVLHNNDLNQ
CCCCCCCCCCCCCCCCEEEEECCCCEECCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCH
VTWEMRAMEGSPKFAESQTLPDVDHARFARGLDLRGDNIEDPAQIAGAWDRALSADRPTV
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEE
LDVRCDPDVPPIPPHATVDEVTSLVGAVVGGDEDTAGFTRQGIKQKLQQYVPLPKRDRP
EEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSTVGDILLSRLREWGVQQVFGYPGDGINGLLAAWRCADNDPQFVQARHEEMAAFEAVG
CCHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
FAKFSGQLGVCTATSGPGAIHLLNGLYDAKLDHVPVLAIVGQADRSAMGGSYQQEVDLLS
HHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCCHHHHHHHHH
LYKDVCSDYVQQCMVPQQLPNLVDRAIRIALSEATPTAIIVPSDVFNLGYEAPVHAFKQV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCHHHHHHHHH
PSSLGMSGSRSIPSPDALRAAADLLNSGKKVALLVGQGARGCVAELTALADLLGAGAAKA
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHH
LLGKDVLPDDLPWVTGSIGLLGTTASWHLMRDCDTLLTVGSNFPYTQFLPDLGQARAVQI
HHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEE
DRSGKWIGMRYPYELNLVGDAKDTLQALIPLLERKPDRSWREHIEKKTRGWWTTVQRTAM
ECCCCEEEEECCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHC
TNADPVNPMRIFWELTKRMPPNAIITADSGSSANWYARHLRFTDGVRGSLSGTLATMGPG
CCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHEECCCCCCCCCCCCHHHCCCC
VPYAIGAKWAHPDRPGIAFVGDGAMQMNGMNELITIARYWRQWADPRLVVVVLHNNDLNQ
CCCCCCCCCCCCCCCCEEEEECCCCEECCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCH
VTWEMRAMEGSPKFAESQTLPDVDHARFARGLDLRGDNIEDPAQIAGAWDRALSADRPTV
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEE
LDVRCDPDVPPIPPHATVDEVTSLVGAVVGGDEDTAGFTRQGIKQKLQQYVPLPKRDRP
EEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0017 {lecithin}} 0.03 {sodium} 11 {pyruvate}} 10 {pyruvate}} 0.0022 {lysolecithin}} 2 {ferricyanide}} 0.0012 {thiamine} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]