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The map label for this gene is glcB

Identifier: 169629495

GI number: 169629495

Start: 2466805

End: 2469012

Strand: Reverse

Name: glcB

Synonym: MAB_2409c

Alternate gene names: 169629495

Gene position: 2469012-2466805 (Counterclockwise)

Preceding gene: 169629496

Following gene: 169629494

Centisome position: 48.73

GC content: 64.58

Gene sequence:

>2208_bases
ATGAGCGATCGCGTCGATGTGGGCAACCTACGCGTGGCGCGGGTGCTGTATGACTTCATCACCAACGAGGCCCTTCCGGG
CACCGGTGTGGATGCCGACGCTTTCTGGGCTGGAGTCGACAAAGTAGTCACCGATTTGGCTCCGCGGAACCGCGAGCTGC
TCGAGCGCCGGGACGACCTGCAAACTCAGGTCGATCGCTGGCACCGGCAGCGCGCCATCGAACCGCTCGATGGCGCGGCG
TACCAGGACTTCCTGACCGAGATCGGATACCTGGTTCCCGAGCCCGAAGATTTCACCATCACCACCGCCAATGTGGACGA
CGAAATCACCACCACGGCCGGTCCGCAGCTGGTGGTGCCGATCCTGAACGCGCGCTTCGCGCTCAACGCCGCCAACGCGC
GCTGGGGCTCCCTCTACGACGCGCTATACGGCACCGATGTCATCCCGGAAGCCGATGGCGCCGAAAAGGCGTCCGCTGAC
ACTGGTGGGTACAACAAGGTCCGCGGTGACAAGGTCATCGCCTACGCCCGCGAGGTGCTCGACGGTGCTGCGCCGCTGGC
ATCCGGCTCGTGGAAGGACGCCGTCGGCCTGAAGATCGACGACGGCCAGCTGCTCGTCGAGCTGGGCGACGAGTTCTCGA
CGGGCCTGCAGAACCCCGATCAATTCGTGGGCTACACCGGCAAGCTCGGTAAGCCGCAGTGGTCGGTGCTACTGATCAAC
CACGGCCTGCATATCGAGGTGCTGATCGACCCCGACTCCCCCATCGGTTCCACCGACAAGGCCGGCATCAAGGATGTGGT
GCTGGAGTCGGCGATCACCACGATCATGGACTTCGAGGACTCTGTCGCGGCCGTGGACGCCGAGGACAAGGTGCTCGGGT
ACCGAAACTGGTTGGGCCTCAACAAGGGCGACCTGTCCGAAGAGGTGTCCAAGGGCGGCAAGACGTTCACCCGCGTGCTC
AACGAGGACCGTGTCTTCACCACCCCCGACGGCAAGGGCGAGCTGACGCTGCCCGGTCGCAGCCTGCTATTCGTCCGCAA
CGTCGGTCATCTGATGACCAATGACGCCATCGTGGACGCCGCGGGCAACGAGATGCCCGAGGGCATCCAGGACGCGCTGT
TCACCAGTCTGATCGCCATCCACGGCCTGCGCTCGGGCAAGAAGAACGGCCCGCTCAAGAACAGCCGCACCGGCTCGATC
TACATCGTGAAGCCCAAGATGCACGGGCCCGAGGAAGTGGCCTTCACCGCCGAACTGTTCGGCCGCGTGGAAGAGGTGCT
CGGATTGCCGCACGCCACCCTCAAGGTCGGCATCATGGACGAGGAGCGCCGCACCACCGTAAATCTGAAGGCAGCCATCA
AGGCCGCTTCGGATCGGGTGGTCTTCATCAACACCGGATTCCTGGACCGCACTGGCGACGAGATCCACACCTCCATGGAA
GCCGGCCCGATGTGTCGTAAGGCCGTCATGAAGTCGCAACCGTGGATCCTGGCGTACGAGGACCACAACGTGGATACGGG
GCTGGGCGCCGGATTGGCCGGCAAGGCACAGATCGGCAAGGGCATGTGGGCCATGCCGGACCTGATGGCGGACATGGTGG
CGCAGAAGATCGCGCAGCCACGCGCTGGTGCGACCACGGCGTGGGTGCCGTCCCCCACCGCGGCGACTCTGCACGCGCTG
CACTACCACCAGGTCGACGTCAAGGCGGTGGACCAGGAGCTCGCCGGTAAGCACCGCGCCAAGCTGGAGTCTTTGCTCAC
CATCCCGCTGGCCGAGTCGCGCCATGACTGGACCGCCGAAGACATCCGCGAGGAAATCGACAACAACTGCCAGTCGATCC
TGGGTTACGTGGTCCGCTGGATCGACCAGGGCGTCGGCTGCTCCAAGGTGCCGGATATCCATGACGTGGCACTCATGGAA
GACCGCGCCACCCTACGCATCTCCAGCCAGCTACTGGCCAACTGGTTGCGCCACGATGTGATCACCGAGGACGAGGTGGT
CGAGGGGCTCAAGCGGATGGCTCCGGTTGTCGACCGGCAGAACGCGGGCGACAAGGAGTACCGCCCGATGGCTCCGGACT
TCGACAGCAACATTGCCTTCCAGGCCGCCAAGGAACTCATCCTGGAGGGCAAGGACCAGCCGAACGGTTACACCGAGCCG
ATCTTGCACCGGCGGCGCCGTGAGTACAAGGCCGCCGCTGCCAAGTAG

Upstream 100 bases:

>100_bases
AGCGGTTTCGGGGTTGGTGGCTACCAATGAGTAGGATCAATGTTTACCGATTTCGCAAGTACGTCCGGTCCCAGACCCCC
GACAACACAGAGGAGTTGCG

Downstream 100 bases:

>100_bases
CGGATGGGTACCCGACGTGTCTCTACCTGTGGCGCCGGGTACGCCTCCGCGTCTATTTGGACGCGGTTTGGTTTCGCGTA
CCGGGGTTTCGGCGGTCAGG

Product: malate synthase G

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 735; Mature: 734

Protein sequence:

>735_residues
MSDRVDVGNLRVARVLYDFITNEALPGTGVDADAFWAGVDKVVTDLAPRNRELLERRDDLQTQVDRWHRQRAIEPLDGAA
YQDFLTEIGYLVPEPEDFTITTANVDDEITTTAGPQLVVPILNARFALNAANARWGSLYDALYGTDVIPEADGAEKASAD
TGGYNKVRGDKVIAYAREVLDGAAPLASGSWKDAVGLKIDDGQLLVELGDEFSTGLQNPDQFVGYTGKLGKPQWSVLLIN
HGLHIEVLIDPDSPIGSTDKAGIKDVVLESAITTIMDFEDSVAAVDAEDKVLGYRNWLGLNKGDLSEEVSKGGKTFTRVL
NEDRVFTTPDGKGELTLPGRSLLFVRNVGHLMTNDAIVDAAGNEMPEGIQDALFTSLIAIHGLRSGKKNGPLKNSRTGSI
YIVKPKMHGPEEVAFTAELFGRVEEVLGLPHATLKVGIMDEERRTTVNLKAAIKAASDRVVFINTGFLDRTGDEIHTSME
AGPMCRKAVMKSQPWILAYEDHNVDTGLGAGLAGKAQIGKGMWAMPDLMADMVAQKIAQPRAGATTAWVPSPTAATLHAL
HYHQVDVKAVDQELAGKHRAKLESLLTIPLAESRHDWTAEDIREEIDNNCQSILGYVVRWIDQGVGCSKVPDIHDVALME
DRATLRISSQLLANWLRHDVITEDEVVEGLKRMAPVVDRQNAGDKEYRPMAPDFDSNIAFQAAKELILEGKDQPNGYTEP
ILHRRRREYKAAAAK

Sequences:

>Translated_735_residues
MSDRVDVGNLRVARVLYDFITNEALPGTGVDADAFWAGVDKVVTDLAPRNRELLERRDDLQTQVDRWHRQRAIEPLDGAA
YQDFLTEIGYLVPEPEDFTITTANVDDEITTTAGPQLVVPILNARFALNAANARWGSLYDALYGTDVIPEADGAEKASAD
TGGYNKVRGDKVIAYAREVLDGAAPLASGSWKDAVGLKIDDGQLLVELGDEFSTGLQNPDQFVGYTGKLGKPQWSVLLIN
HGLHIEVLIDPDSPIGSTDKAGIKDVVLESAITTIMDFEDSVAAVDAEDKVLGYRNWLGLNKGDLSEEVSKGGKTFTRVL
NEDRVFTTPDGKGELTLPGRSLLFVRNVGHLMTNDAIVDAAGNEMPEGIQDALFTSLIAIHGLRSGKKNGPLKNSRTGSI
YIVKPKMHGPEEVAFTAELFGRVEEVLGLPHATLKVGIMDEERRTTVNLKAAIKAASDRVVFINTGFLDRTGDEIHTSME
AGPMCRKAVMKSQPWILAYEDHNVDTGLGAGLAGKAQIGKGMWAMPDLMADMVAQKIAQPRAGATTAWVPSPTAATLHAL
HYHQVDVKAVDQELAGKHRAKLESLLTIPLAESRHDWTAEDIREEIDNNCQSILGYVVRWIDQGVGCSKVPDIHDVALME
DRATLRISSQLLANWLRHDVITEDEVVEGLKRMAPVVDRQNAGDKEYRPMAPDFDSNIAFQAAKELILEGKDQPNGYTEP
ILHRRRREYKAAAAK
>Mature_734_residues
SDRVDVGNLRVARVLYDFITNEALPGTGVDADAFWAGVDKVVTDLAPRNRELLERRDDLQTQVDRWHRQRAIEPLDGAAY
QDFLTEIGYLVPEPEDFTITTANVDDEITTTAGPQLVVPILNARFALNAANARWGSLYDALYGTDVIPEADGAEKASADT
GGYNKVRGDKVIAYAREVLDGAAPLASGSWKDAVGLKIDDGQLLVELGDEFSTGLQNPDQFVGYTGKLGKPQWSVLLINH
GLHIEVLIDPDSPIGSTDKAGIKDVVLESAITTIMDFEDSVAAVDAEDKVLGYRNWLGLNKGDLSEEVSKGGKTFTRVLN
EDRVFTTPDGKGELTLPGRSLLFVRNVGHLMTNDAIVDAAGNEMPEGIQDALFTSLIAIHGLRSGKKNGPLKNSRTGSIY
IVKPKMHGPEEVAFTAELFGRVEEVLGLPHATLKVGIMDEERRTTVNLKAAIKAASDRVVFINTGFLDRTGDEIHTSMEA
GPMCRKAVMKSQPWILAYEDHNVDTGLGAGLAGKAQIGKGMWAMPDLMADMVAQKIAQPRAGATTAWVPSPTAATLHALH
YHQVDVKAVDQELAGKHRAKLESLLTIPLAESRHDWTAEDIREEIDNNCQSILGYVVRWIDQGVGCSKVPDIHDVALMED
RATLRISSQLLANWLRHDVITEDEVVEGLKRMAPVVDRQNAGDKEYRPMAPDFDSNIAFQAAKELILEGKDQPNGYTEPI
LHRRRREYKAAAAK

Specific function: Accounts For Almost The Entire Malate-Synthesizing Activity In Cells Metabolizing Glyoxylate. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family. GlcB subfamily

Homologues:

Organism=Escherichia coli, GI1789348, Length=735, Percent_Identity=57.9591836734694, Blast_Score=850, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MASZ_MYCA9 (B1MB69)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703144.1
- ProteinModelPortal:   B1MB69
- SMR:   B1MB69
- EnsemblBacteria:   EBMYCT00000004361
- GeneID:   5964919
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_2409c
- GeneTree:   EBGT00070000032288
- HOGENOM:   HBG350005
- OMA:   PKMHGPD
- ProtClustDB:   PRK02999
- BioCyc:   MABS561007:MAB_2409C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00641
- InterPro:   IPR011076
- InterPro:   IPR001465
- InterPro:   IPR006253
- PANTHER:   PTHR21631:SF1
- TIGRFAMs:   TIGR01345

Pfam domain/function: PF01274 Malate_synthase; SSF51645 Malat_synth_like

EC number: =2.3.3.9

Molecular weight: Translated: 80390; Mature: 80259

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: ACT_SITE 346-346 ACT_SITE 641-641

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRVDVGNLRVARVLYDFITNEALPGTGVDADAFWAGVDKVVTDLAPRNRELLERRDDL
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
QTQVDRWHRQRAIEPLDGAAYQDFLTEIGYLVPEPEDFTITTANVDDEITTTAGPQLVVP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEEEE
ILNARFALNAANARWGSLYDALYGTDVIPEADGAEKASADTGGYNKVRGDKVIAYAREVL
ECCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
DGAAPLASGSWKDAVGLKIDDGQLLVELGDEFSTGLQNPDQFVGYTGKLGKPQWSVLLIN
HCCCCCCCCCCCCCCCEEECCCCEEEEECCHHHHCCCCCHHHCCCCCCCCCCCEEEEEEE
HGLHIEVLIDPDSPIGSTDKAGIKDVVLESAITTIMDFEDSVAAVDAEDKVLGYRNWLGL
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHCCC
NKGDLSEEVSKGGKTFTRVLNEDRVFTTPDGKGELTLPGRSLLFVRNVGHLMTNDAIVDA
CCCCHHHHHHCCHHHHHHHHCCCCEEECCCCCCEEEECCCCEEEEEHHHHHHCCCCEEEC
AGNEMPEGIQDALFTSLIAIHGLRSGKKNGPLKNSRTGSIYIVKPKMHGPEEVAFTAELF
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
GRVEEVLGLPHATLKVGIMDEERRTTVNLKAAIKAASDRVVFINTGFLDRTGDEIHTSME
HHHHHHHCCCCCCEEEEEECCCCCCEEEHHHHEECCCCCEEEEECCCCCCCCHHHHHHHH
AGPMCRKAVMKSQPWILAYEDHNVDTGLGAGLAGKAQIGKGMWAMPDLMADMVAQKIAQP
CCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCC
RAGATTAWVPSPTAATLHALHYHQVDVKAVDQELAGKHRAKLESLLTIPLAESRHDWTAE
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
DIREEIDNNCQSILGYVVRWIDQGVGCSKVPDIHDVALMEDRATLRISSQLLANWLRHDV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHC
ITEDEVVEGLKRMAPVVDRQNAGDKEYRPMAPDFDSNIAFQAAKELILEGKDQPNGYTEP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHH
ILHRRRREYKAAAAK
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDRVDVGNLRVARVLYDFITNEALPGTGVDADAFWAGVDKVVTDLAPRNRELLERRDDL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
QTQVDRWHRQRAIEPLDGAAYQDFLTEIGYLVPEPEDFTITTANVDDEITTTAGPQLVVP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEEEE
ILNARFALNAANARWGSLYDALYGTDVIPEADGAEKASADTGGYNKVRGDKVIAYAREVL
ECCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
DGAAPLASGSWKDAVGLKIDDGQLLVELGDEFSTGLQNPDQFVGYTGKLGKPQWSVLLIN
HCCCCCCCCCCCCCCCEEECCCCEEEEECCHHHHCCCCCHHHCCCCCCCCCCCEEEEEEE
HGLHIEVLIDPDSPIGSTDKAGIKDVVLESAITTIMDFEDSVAAVDAEDKVLGYRNWLGL
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHCCC
NKGDLSEEVSKGGKTFTRVLNEDRVFTTPDGKGELTLPGRSLLFVRNVGHLMTNDAIVDA
CCCCHHHHHHCCHHHHHHHHCCCCEEECCCCCCEEEECCCCEEEEEHHHHHHCCCCEEEC
AGNEMPEGIQDALFTSLIAIHGLRSGKKNGPLKNSRTGSIYIVKPKMHGPEEVAFTAELF
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
GRVEEVLGLPHATLKVGIMDEERRTTVNLKAAIKAASDRVVFINTGFLDRTGDEIHTSME
HHHHHHHCCCCCCEEEEEECCCCCCEEEHHHHEECCCCCEEEEECCCCCCCCHHHHHHHH
AGPMCRKAVMKSQPWILAYEDHNVDTGLGAGLAGKAQIGKGMWAMPDLMADMVAQKIAQP
CCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCC
RAGATTAWVPSPTAATLHALHYHQVDVKAVDQELAGKHRAKLESLLTIPLAESRHDWTAE
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
DIREEIDNNCQSILGYVVRWIDQGVGCSKVPDIHDVALMEDRATLRISSQLLANWLRHDV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHC
ITEDEVVEGLKRMAPVVDRQNAGDKEYRPMAPDFDSNIAFQAAKELILEGKDQPNGYTEP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHH
ILHRRRREYKAAAAK
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA