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The map label for this gene is dhbE [H]

Identifier: 169629333

GI number: 169629333

Start: 2274357

End: 2276009

Strand: Reverse

Name: dhbE [H]

Synonym: MAB_2247c

Alternate gene names: 169629333

Gene position: 2276009-2274357 (Counterclockwise)

Preceding gene: 169629340

Following gene: 169629323

Centisome position: 44.92

GC content: 64.79

Gene sequence:

>1653_bases
ATGACTCAGCCCAGATCGACCACCACCGCGTCGTCAGCCGCAACCCCCGCACTGGATGGTTTCGTCCCGTTTCCCTCGGT
ACGCGCCGACGAATATCGCCGGGCCGGGTACTGGACGGGCAACACCGTCGAATCGCTGCTTCGCGTGTCCGCGGCCAGGC
GGCCATGCCATCCGGCTGTGATCGACGAACACCGCGCGCTGAGCTATCGAGAACTGGACGAGGCAGCCGACAATGCCGCA
CACGCCTTCCTCCGGCTGGGGATAGCGCCCGGCGATCGGGTGCTGCTCCAACTCCCCAATCGGGCATCGTTTGCCGTCGC
GCTATTCGGCTTGATGCGTGCCGGCGCAATACCCGTGATGTGCCTTCCCGGGCACCGGGCCGCCGAACTGTCCCACTTCA
TCGAGGTGGCCGACGCTGTCGGATTGGTTATCCCCGATTCGGCCGGTGGATTCGATTACCGCGCGCTGGCGGTCGCGCTG
TCCCAGCGGCATCCGCGCCTGCGCACGGTGGTCGTGGAGGGTGATGCGGGCCCCTTCGCATCCTGGACGTCGCTGCTGAA
AAGTGTTGAGCCGCAGGGTCCGCTACCTACCGTGCAGACGGACACTCCTGCCCTACTGCTGGTGTCCGGCGGCACCACCG
CAGCACCCAAGCTGATCCCTCGCACCCACGAGGACTACGTCTACAACGCCACACAGGCAGCACGGGTCTGCGGTCTGACC
CAGGACGACGTTTACCTCGTGGCACTGCCCGCCGGCCATAATTTCCCCCTGGCTTGCCCGGGCTTGCTTGGTGCGATAAG
CACCGGTGCGACCACGGTTTTCCTCTCGGATCCGAGTCCGGAGTCGGCTTTCGCGACCATCGCTCGCCATCGAGTGTCGG
TGACGGCAGTGGTGCCATCGCTTGCACAGCTATGGGCTCAAGCCACCGCGTGGGAGCCGGTGCTGCCGGAAACACTGAGG
CTGCTCCAGGTTGGTGGCGCCAAACTCGGCGCCGACGACGCACGAGCGGTACGAGAGTCGCTCACCCAGGGACTGCAACA
GGTTTTCGGCATGGCAGAGGGCCTGTTATGCCTCACACAGCCAGGCGATCCCACCGATATCCTGGATAACAGCCAGGGCC
GTCCCATGTGCGACGCGGACGAGGTGCGCATAGTCGACGAGGACGGCGCCGACGTCCCCGCCGGAGAAACCGGGGAACTG
CTCGTGCGTGGGCCATACACCCTCAACGGGTACTACCGCGCCGACGCCGACAACATGCGCTCGTTCACTGTCGACGGTTT
CTATCGCAGCGGTGACCGGGTACGCGCACTACCGGGCGGTTATCTGGAAGTCACCGGACGCATCAAGGATGTCATTCTCC
GTGGCGGGGAATCCATCGCCGCGTTGGATCTCGAATCGCACTTGCAGACCCATCCGGCGGTCTACGCCGCAGCGGCGGTG
GGCCTTCCCGATCAGTATCTTGGCGAGATTGTCTGTGCTGCAATTGTTTTCAAGGGAAAACCGGTCGCCGCCGCCGAACT
CAATCAGCATCTGCAAGCCCGTGGCGCCGCGACCCATTCACGCGTCGACAAACTGGTCGCCGTTTCCGCACTGCCACTGA
CCGCTGTGGGCAAGATCGACAAGCGAGCGCTGCTGGCCTCCCTGACGGGCTAG

Upstream 100 bases:

>100_bases
ACTCGATCTCACCTCGCGTTTTCACTATTAGTACAGGCTGCCCTAACTACGAGGAGGCTACCCTATCTAGACTGCGAGGG
GTCACCCACGAGGAGCCCCG

Downstream 100 bases:

>100_bases
GCCGTCAATCCATCGGGTGCGATGTCAACCACTCAAGCACCTGCTGGGCATGTGTATCCGGCGGAAAGATCGGATAAAAG
ACCTGCTCAATGCGCCCGTC

Product: bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase

Products: NA

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MTQPRSTTTASSAATPALDGFVPFPSVRADEYRRAGYWTGNTVESLLRVSAARRPCHPAVIDEHRALSYRELDEAADNAA
HAFLRLGIAPGDRVLLQLPNRASFAVALFGLMRAGAIPVMCLPGHRAAELSHFIEVADAVGLVIPDSAGGFDYRALAVAL
SQRHPRLRTVVVEGDAGPFASWTSLLKSVEPQGPLPTVQTDTPALLLVSGGTTAAPKLIPRTHEDYVYNATQAARVCGLT
QDDVYLVALPAGHNFPLACPGLLGAISTGATTVFLSDPSPESAFATIARHRVSVTAVVPSLAQLWAQATAWEPVLPETLR
LLQVGGAKLGADDARAVRESLTQGLQQVFGMAEGLLCLTQPGDPTDILDNSQGRPMCDADEVRIVDEDGADVPAGETGEL
LVRGPYTLNGYYRADADNMRSFTVDGFYRSGDRVRALPGGYLEVTGRIKDVILRGGESIAALDLESHLQTHPAVYAAAAV
GLPDQYLGEIVCAAIVFKGKPVAAAELNQHLQARGAATHSRVDKLVAVSALPLTAVGKIDKRALLASLTG

Sequences:

>Translated_550_residues
MTQPRSTTTASSAATPALDGFVPFPSVRADEYRRAGYWTGNTVESLLRVSAARRPCHPAVIDEHRALSYRELDEAADNAA
HAFLRLGIAPGDRVLLQLPNRASFAVALFGLMRAGAIPVMCLPGHRAAELSHFIEVADAVGLVIPDSAGGFDYRALAVAL
SQRHPRLRTVVVEGDAGPFASWTSLLKSVEPQGPLPTVQTDTPALLLVSGGTTAAPKLIPRTHEDYVYNATQAARVCGLT
QDDVYLVALPAGHNFPLACPGLLGAISTGATTVFLSDPSPESAFATIARHRVSVTAVVPSLAQLWAQATAWEPVLPETLR
LLQVGGAKLGADDARAVRESLTQGLQQVFGMAEGLLCLTQPGDPTDILDNSQGRPMCDADEVRIVDEDGADVPAGETGEL
LVRGPYTLNGYYRADADNMRSFTVDGFYRSGDRVRALPGGYLEVTGRIKDVILRGGESIAALDLESHLQTHPAVYAAAAV
GLPDQYLGEIVCAAIVFKGKPVAAAELNQHLQARGAATHSRVDKLVAVSALPLTAVGKIDKRALLASLTG
>Mature_549_residues
TQPRSTTTASSAATPALDGFVPFPSVRADEYRRAGYWTGNTVESLLRVSAARRPCHPAVIDEHRALSYRELDEAADNAAH
AFLRLGIAPGDRVLLQLPNRASFAVALFGLMRAGAIPVMCLPGHRAAELSHFIEVADAVGLVIPDSAGGFDYRALAVALS
QRHPRLRTVVVEGDAGPFASWTSLLKSVEPQGPLPTVQTDTPALLLVSGGTTAAPKLIPRTHEDYVYNATQAARVCGLTQ
DDVYLVALPAGHNFPLACPGLLGAISTGATTVFLSDPSPESAFATIARHRVSVTAVVPSLAQLWAQATAWEPVLPETLRL
LQVGGAKLGADDARAVRESLTQGLQQVFGMAEGLLCLTQPGDPTDILDNSQGRPMCDADEVRIVDEDGADVPAGETGELL
VRGPYTLNGYYRADADNMRSFTVDGFYRSGDRVRALPGGYLEVTGRIKDVILRGGESIAALDLESHLQTHPAVYAAAAVG
LPDQYLGEIVCAAIVFKGKPVAAAELNQHLQARGAATHSRVDKLVAVSALPLTAVGKIDKRALLASLTG

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=534, Percent_Identity=28.2771535580524, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI187761343, Length=534, Percent_Identity=28.2771535580524, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI38505220, Length=521, Percent_Identity=25.3358925143954, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI156151445, Length=545, Percent_Identity=26.0550458715596, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI115511026, Length=502, Percent_Identity=26.0956175298805, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI122937307, Length=525, Percent_Identity=24.7619047619048, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI42544132, Length=520, Percent_Identity=22.8846153846154, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI58082049, Length=508, Percent_Identity=24.0157480314961, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI157311624, Length=494, Percent_Identity=22.8744939271255, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI157311622, Length=494, Percent_Identity=22.8744939271255, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI28416953, Length=524, Percent_Identity=23.0916030534351, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI1786810, Length=526, Percent_Identity=40.1140684410646, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI145693145, Length=528, Percent_Identity=28.030303030303, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788107, Length=534, Percent_Identity=28.2771535580524, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI221142682, Length=491, Percent_Identity=27.4949083503055, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790505, Length=525, Percent_Identity=24, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1786529, Length=539, Percent_Identity=23.5621521335807, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1786801, Length=542, Percent_Identity=26.0147601476015, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71994703, Length=513, Percent_Identity=24.5614035087719, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71994694, Length=513, Percent_Identity=24.5614035087719, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71994690, Length=513, Percent_Identity=24.5614035087719, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=511, Percent_Identity=27.3972602739726, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=503, Percent_Identity=25.2485089463221, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=508, Percent_Identity=24.2125984251968, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=22.4899598393574, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=531, Percent_Identity=23.1638418079096, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=369, Percent_Identity=25.2032520325203, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=409, Percent_Identity=24.6943765281174, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17558820, Length=510, Percent_Identity=21.9607843137255, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71982997, Length=540, Percent_Identity=23.7037037037037, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71983001, Length=530, Percent_Identity=23.7735849056604, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=348, Percent_Identity=27.8735632183908, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323182, Length=560, Percent_Identity=24.2857142857143, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24581924, Length=521, Percent_Identity=26.679462571977, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI18859661, Length=514, Percent_Identity=24.3190661478599, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21355225, Length=529, Percent_Identity=23.8185255198488, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355181, Length=492, Percent_Identity=22.7642276422764, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648253, Length=353, Percent_Identity=25.7790368271955, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648255, Length=353, Percent_Identity=25.7790368271955, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24656500, Length=508, Percent_Identity=22.6377952755905, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550496, Length=569, Percent_Identity=23.9015817223199, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922652, Length=493, Percent_Identity=22.1095334685598, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45552801, Length=569, Percent_Identity=23.9015817223199, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648260, Length=347, Percent_Identity=24.4956772334294, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356947, Length=322, Percent_Identity=23.6024844720497, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.-

Molecular weight: Translated: 58104; Mature: 57972

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPRSTTTASSAATPALDGFVPFPSVRADEYRRAGYWTGNTVESLLRVSAARRPCHPAV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCH
IDEHRALSYRELDEAADNAAHAFLRLGIAPGDRVLLQLPNRASFAVALFGLMRAGAIPVM
HCCCCCCHHHHHHHHHHHHHEEHHEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEE
CLPGHRAAELSHFIEVADAVGLVIPDSAGGFDYRALAVALSQRHPRLRTVVVEGDAGPFA
EECCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHH
SWTSLLKSVEPQGPLPTVQTDTPALLLVSGGTTAAPKLIPRTHEDYVYNATQAARVCGLT
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECHHHHHHHHCCC
QDDVYLVALPAGHNFPLACPGLLGAISTGATTVFLSDPSPESAFATIARHRVSVTAVVPS
CCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHEEHHHHHHH
LAQLWAQATAWEPVLPETLRLLQVGGAKLGADDARAVRESLTQGLQQVFGMAEGLLCLTQ
HHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
PGDPTDILDNSQGRPMCDADEVRIVDEDGADVPAGETGELLVRGPYTLNGYYRADADNMR
CCCCHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCEEECCEEECCCCCCC
SFTVDGFYRSGDRVRALPGGYLEVTGRIKDVILRGGESIAALDLESHLQTHPAVYAAAAV
EEEECCEECCCCEEEECCCCEEEECHHHHHHHHCCCCCEEEEEHHHHHHHCCHHHHHHHC
GLPDQYLGEIVCAAIVFKGKPVAAAELNQHLQARGAATHSRVDKLVAVSALPLTAVGKID
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHCCCH
KRALLASLTG
HHHHHHHCCC
>Mature Secondary Structure 
TQPRSTTTASSAATPALDGFVPFPSVRADEYRRAGYWTGNTVESLLRVSAARRPCHPAV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCH
IDEHRALSYRELDEAADNAAHAFLRLGIAPGDRVLLQLPNRASFAVALFGLMRAGAIPVM
HCCCCCCHHHHHHHHHHHHHEEHHEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEE
CLPGHRAAELSHFIEVADAVGLVIPDSAGGFDYRALAVALSQRHPRLRTVVVEGDAGPFA
EECCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHH
SWTSLLKSVEPQGPLPTVQTDTPALLLVSGGTTAAPKLIPRTHEDYVYNATQAARVCGLT
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECHHHHHHHHCCC
QDDVYLVALPAGHNFPLACPGLLGAISTGATTVFLSDPSPESAFATIARHRVSVTAVVPS
CCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHEEHHHHHHH
LAQLWAQATAWEPVLPETLRLLQVGGAKLGADDARAVRESLTQGLQQVFGMAEGLLCLTQ
HHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
PGDPTDILDNSQGRPMCDADEVRIVDEDGADVPAGETGELLVRGPYTLNGYYRADADNMR
CCCCHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCEEECCEEECCCCCCC
SFTVDGFYRSGDRVRALPGGYLEVTGRIKDVILRGGESIAALDLESHLQTHPAVYAAAAV
EEEECCEECCCCEEEECCCCEEEECHHHHHHHHCCCCCEEEEEHHHHHHHCCHHHHHHHC
GLPDQYLGEIVCAAIVFKGKPVAAAELNQHLQARGAATHSRVDKLVAVSALPLTAVGKID
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHCCCH
KRALLASLTG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]