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The map label for this gene is polA [C]

Identifier: 169629277

GI number: 169629277

Start: 2206370

End: 2207383

Strand: Reverse

Name: polA [C]

Synonym: MAB_2191c

Alternate gene names: 169629277

Gene position: 2207383-2206370 (Counterclockwise)

Preceding gene: 169629279

Following gene: 169629268

Centisome position: 43.56

GC content: 68.44

Gene sequence:

>1014_bases
GTGTCAGAAGACCCAGCCCGAACCGTTCCCGCCAGACCCGTCTTGCTGCTCGACGGCGCCAGCCTGTGGTTCCGTTCCTA
CTTCGGGGTTCCCGATTCCATCACGTCACCCGACGGCAGACCGGTCAACGCCGTCCGCGGCTTCCTCGACACGGTGTCAC
AGCTGATCACCCTGTATCGGCCTCACCGGCTCGCGGTATGCCTCGATCTGGATTGGCGCCCGGCATGGCGAGTGGCCGCG
ATTCCGTCGTACAAGGCGCACCGCGTGGTCACGGATGATCCGCTTGCGGGAAGACCATCAGACACCGTGGGCGCGCAGAC
TGTCGATATCGAGGAGGTGCCCGACGAGCTGACCCCTCAGGTCGACATGATCGCCGACATACTCGATGCCTTCGGCATCG
CATCGGCAGGAGCTGCCGGCTTCGAGGCCGACGACGTGCTGGGAACCCTTGCGGCCAATGAGCGCCGGGACCCGGTCATC
GTGGTCAGCGGCGATCGCGACCTGCTGCAGGTCGTCGCCGACGTCCCGGTGCCCGTTCGCGTCTTCTACATGGGCCGCGG
ATTCGCCAAGGCCACCCTGTTCGGCCCGACCGAAGTGGCCGAGCAGTACGGTGTGCCCCTCGCGCGCGCCGGCGCGGCCT
ATGCCGAGCTGGCCTTGTTGCGTGGCGACCCGTCCGACGGTCTACCCGGCGTCCCGGGCGTGGGCGAGAAGACCGCGGCC
AAACTTCTCGCCGAGTTTGGGTCGCTGGACGGCATACTCACCGCAGCCGATGATCCGAAATCCGGTCTGGCCAAGGCCGT
TCGCGCCAAGCTGCAGGCAGCGACCGTCTACATGGCCGCCGCCGAGCCGGTGGTGCGGGTCGCGGTGGACGCGCCGGTAA
TTGTCGACCGCGACAGCGACGCGCTGCCGCTGACGGCGCGCGATCCGCGGCGAGTCGCCGAGCTGGGCGCCGAACTGGGC
ATCGGGTCGGCGGTCACCCGTCTGCAAAAGGCGCTTGACGGCCTGCCCCGGTAG

Upstream 100 bases:

>100_bases
CGGCCTCCTGGGCCGCGCGTCGCAGTCGGTCGGCGTAGACGTCGGATTCGAAACGCTGGGGTGCCATACGGTCCAGGCTA
TCTGGCATAGGTTGAGTGCT

Downstream 100 bases:

>100_bases
TACAAACAACGCCGAGTGTGCGGATCTGCACACTCGGCGTTGTTGAGGAGGGAACCGATTACTTGGGGCGACCGACCTCG
TAGGTGCCCTTGTCGTTCTG

Product: 5'-3' exonuclease

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSEDPARTVPARPVLLLDGASLWFRSYFGVPDSITSPDGRPVNAVRGFLDTVSQLITLYRPHRLAVCLDLDWRPAWRVAA
IPSYKAHRVVTDDPLAGRPSDTVGAQTVDIEEVPDELTPQVDMIADILDAFGIASAGAAGFEADDVLGTLAANERRDPVI
VVSGDRDLLQVVADVPVPVRVFYMGRGFAKATLFGPTEVAEQYGVPLARAGAAYAELALLRGDPSDGLPGVPGVGEKTAA
KLLAEFGSLDGILTAADDPKSGLAKAVRAKLQAATVYMAAAEPVVRVAVDAPVIVDRDSDALPLTARDPRRVAELGAELG
IGSAVTRLQKALDGLPR

Sequences:

>Translated_337_residues
MSEDPARTVPARPVLLLDGASLWFRSYFGVPDSITSPDGRPVNAVRGFLDTVSQLITLYRPHRLAVCLDLDWRPAWRVAA
IPSYKAHRVVTDDPLAGRPSDTVGAQTVDIEEVPDELTPQVDMIADILDAFGIASAGAAGFEADDVLGTLAANERRDPVI
VVSGDRDLLQVVADVPVPVRVFYMGRGFAKATLFGPTEVAEQYGVPLARAGAAYAELALLRGDPSDGLPGVPGVGEKTAA
KLLAEFGSLDGILTAADDPKSGLAKAVRAKLQAATVYMAAAEPVVRVAVDAPVIVDRDSDALPLTARDPRRVAELGAELG
IGSAVTRLQKALDGLPR
>Mature_336_residues
SEDPARTVPARPVLLLDGASLWFRSYFGVPDSITSPDGRPVNAVRGFLDTVSQLITLYRPHRLAVCLDLDWRPAWRVAAI
PSYKAHRVVTDDPLAGRPSDTVGAQTVDIEEVPDELTPQVDMIADILDAFGIASAGAAGFEADDVLGTLAANERRDPVIV
VSGDRDLLQVVADVPVPVRVFYMGRGFAKATLFGPTEVAEQYGVPLARAGAAYAELALLRGDPSDGLPGVPGVGEKTAAK
LLAEFGSLDGILTAADDPKSGLAKAVRAKLQAATVYMAAAEPVVRVAVDAPVIVDRDSDALPLTARDPRRVAELGAELGI
GSAVTRLQKALDGLPR

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=270, Percent_Identity=34.4444444444444, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918
- InterPro:   IPR003583 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 35502; Mature: 35371

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDPARTVPARPVLLLDGASLWFRSYFGVPDSITSPDGRPVNAVRGFLDTVSQLITLYR
CCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
PHRLAVCLDLDWRPAWRVAAIPSYKAHRVVTDDPLAGRPSDTVGAQTVDIEEVPDELTPQ
CCEEEEEEECCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCEEEEHHHCCHHHCCH
VDMIADILDAFGIASAGAAGFEADDVLGTLAANERRDPVIVVSGDRDLLQVVADVPVPVR
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCEE
VFYMGRGFAKATLFGPTEVAEQYGVPLARAGAAYAELALLRGDPSDGLPGVPGVGEKTAA
EEEECCCEEEEEECCHHHHHHHHCCCHHHCCHHHHHHHEEECCCCCCCCCCCCCCHHHHH
KLLAEFGSLDGILTAADDPKSGLAKAVRAKLQAATVYMAAAEPVVRVAVDAPVIVDRDSD
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCEEEECCCC
ALPLTARDPRRVAELGAELGIGSAVTRLQKALDGLPR
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEDPARTVPARPVLLLDGASLWFRSYFGVPDSITSPDGRPVNAVRGFLDTVSQLITLYR
CCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
PHRLAVCLDLDWRPAWRVAAIPSYKAHRVVTDDPLAGRPSDTVGAQTVDIEEVPDELTPQ
CCEEEEEEECCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCEEEEHHHCCHHHCCH
VDMIADILDAFGIASAGAAGFEADDVLGTLAANERRDPVIVVSGDRDLLQVVADVPVPVR
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCEE
VFYMGRGFAKATLFGPTEVAEQYGVPLARAGAAYAELALLRGDPSDGLPGVPGVGEKTAA
EEEECCCEEEEEECCHHHHHHHHCCCHHHCCHHHHHHHEEECCCCCCCCCCCCCCHHHHH
KLLAEFGSLDGILTAADDPKSGLAKAVRAKLQAATVYMAAAEPVVRVAVDAPVIVDRDSD
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCEEEECCCC
ALPLTARDPRRVAELGAELGIGSAVTRLQKALDGLPR
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]