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The map label for this gene is trmI [H]

Identifier: 169629245

GI number: 169629245

Start: 2173762

End: 2174598

Strand: Direct

Name: trmI [H]

Synonym: MAB_2159

Alternate gene names: 169629245

Gene position: 2173762-2174598 (Clockwise)

Preceding gene: 169629243

Following gene: 169629248

Centisome position: 42.9

GC content: 66.19

Gene sequence:

>837_bases
ATGTCTCGAACCGGACCGTTCATCGTCGGCGATCGCGTGCAACTGACCGACGCCAAGGGGCGTCACTACACGATGGTGCT
CGAGCCCGGCAAGGAGTTCCACACACATCGTGGGGCCATCGTGCACGACGAGGTGATCGGTATACCCGAGGGCAGCGTGG
TGAAATCCACCAACGGCGACCAGTTTTTGGTGCTGCGTCCGCTGCTGCTCGACTATGTGCTGTCGATGCCGCGCGGTGCT
CAGGTGATCTACCCGAAAGACGCCGCGCAGATCGTGCACGACGGTGACATCTTCCCTGGCGCCCGGGTGCTGGAGGCCGG
CGCCGGATCCGGGGCGCTCACCTGTTCGCTGTTGCGAGCAGTGGGGCCGCAGGGAGCAGTGATCTCCTACGAGATTCGTG
AGGATCACGCCGAACACGCTGTGCGTAACGTCACAACGTTCTTCGGGGAGCGGCCGGACAACTGGGACCTGGTGATCGGC
GATCTCGCCGAGCGACCGGCGGACGCCGGCAGCGTCGATCGCGTGGTCCTCGACATGCTGGCGCCCTGGGAAACTTTGCC
CGCAGTGGCCGAAACGCTGGTGCCCGGCGGCGTCCTGATCGTCTATGTCGCCACCGTCACGCAGCTGTCGCGTGTGGTCG
AGGCGCTGCGTGAGCAGCAATGTTGGACCGAGCCCCGGTCTTGGGAAACGATGCAGCGCGGCTGGAACGTGGTGGGACTG
GCGGTCCGCCCGGAACACAGCATGCGTGGACACACCGCGTTTCTGGTCAGCGCGCGCCGTCTGGCCCCGGGCACGATCAC
CCCGACACCGCTGCGGCGCAAGCGCCTGCCGAGCTAG

Upstream 100 bases:

>100_bases
TGTGCCGAAACACATTGGGTTGGCGTCTTCTTCAGCCCTGCTCAACCCGGGAAACCTGCCCCATTCGCGTCGGGCGGTGC
GCACCTGGCAAGGTGAGCGC

Downstream 100 bases:

>100_bases
CGGATTAGCTACGGGGTAGGTGCCGGTGCCTGCGGGCTAGGGGCGTCGGCGGGCGGCGCCTCCTTCGGGGTCCCTGTGCC
CGCCAGCGATGTTCCGGTCT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(m1A58)-methyltransferase; tRNA(m1A58)MTase [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSRTGPFIVGDRVQLTDAKGRHYTMVLEPGKEFHTHRGAIVHDEVIGIPEGSVVKSTNGDQFLVLRPLLLDYVLSMPRGA
QVIYPKDAAQIVHDGDIFPGARVLEAGAGSGALTCSLLRAVGPQGAVISYEIREDHAEHAVRNVTTFFGERPDNWDLVIG
DLAERPADAGSVDRVVLDMLAPWETLPAVAETLVPGGVLIVYVATVTQLSRVVEALREQQCWTEPRSWETMQRGWNVVGL
AVRPEHSMRGHTAFLVSARRLAPGTITPTPLRRKRLPS

Sequences:

>Translated_278_residues
MSRTGPFIVGDRVQLTDAKGRHYTMVLEPGKEFHTHRGAIVHDEVIGIPEGSVVKSTNGDQFLVLRPLLLDYVLSMPRGA
QVIYPKDAAQIVHDGDIFPGARVLEAGAGSGALTCSLLRAVGPQGAVISYEIREDHAEHAVRNVTTFFGERPDNWDLVIG
DLAERPADAGSVDRVVLDMLAPWETLPAVAETLVPGGVLIVYVATVTQLSRVVEALREQQCWTEPRSWETMQRGWNVVGL
AVRPEHSMRGHTAFLVSARRLAPGTITPTPLRRKRLPS
>Mature_277_residues
SRTGPFIVGDRVQLTDAKGRHYTMVLEPGKEFHTHRGAIVHDEVIGIPEGSVVKSTNGDQFLVLRPLLLDYVLSMPRGAQ
VIYPKDAAQIVHDGDIFPGARVLEAGAGSGALTCSLLRAVGPQGAVISYEIREDHAEHAVRNVTTFFGERPDNWDLVIGD
LAERPADAGSVDRVVLDMLAPWETLPAVAETLVPGGVLIVYVATVTQLSRVVEALREQQCWTEPRSWETMQRGWNVVGLA
VRPEHSMRGHTAFLVSARRLAPGTITPTPLRRKRLPS

Specific function: Catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in tRNA. Is required for cell growth at extreme temperatures [H]

COG id: COG2519

COG function: function code J; tRNA(1-methyladenosine) methyltransferase and related methyltransferases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmI family [H]

Homologues:

Organism=Homo sapiens, GI222831587, Length=229, Percent_Identity=36.2445414847162, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI123173772, Length=239, Percent_Identity=27.6150627615063, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17509459, Length=209, Percent_Identity=27.7511961722488, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322336, Length=226, Percent_Identity=27.8761061946903, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24650257, Length=221, Percent_Identity=28.9592760180996, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014816 [H]

Pfam domain/function: PF08704 GCD14 [H]

EC number: =2.1.1.36 [H]

Molecular weight: Translated: 30416; Mature: 30285

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTGPFIVGDRVQLTDAKGRHYTMVLEPGKEFHTHRGAIVHDEVIGIPEGSVVKSTNGD
CCCCCCEEECCEEEEECCCCCEEEEEECCCHHHHHCCCCEEECCEECCCCCCEEECCCCC
QFLVLRPLLLDYVLSMPRGAQVIYPKDAAQIVHDGDIFPGARVLEAGAGSGALTCSLLRA
EEEEHHHHHHHHHHCCCCCCEEEECCHHHHHEECCCCCCCHHHEECCCCCCHHHHHHHHH
VGPQGAVISYEIREDHAEHAVRNVTTFFGERPDNWDLVIGDLAERPADAGSVDRVVLDML
CCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHCCCCCCCCHHHHHHHHH
APWETLPAVAETLVPGGVLIVYVATVTQLSRVVEALREQQCWTEPRSWETMQRGWNVVGL
CCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEE
AVRPEHSMRGHTAFLVSARRLAPGTITPTPLRRKRLPS
EECCCCCCCCCEEEEEEHHHCCCCCCCCCCHHHCCCCC
>Mature Secondary Structure 
SRTGPFIVGDRVQLTDAKGRHYTMVLEPGKEFHTHRGAIVHDEVIGIPEGSVVKSTNGD
CCCCCEEECCEEEEECCCCCEEEEEECCCHHHHHCCCCEEECCEECCCCCCEEECCCCC
QFLVLRPLLLDYVLSMPRGAQVIYPKDAAQIVHDGDIFPGARVLEAGAGSGALTCSLLRA
EEEEHHHHHHHHHHCCCCCCEEEECCHHHHHEECCCCCCCHHHEECCCCCCHHHHHHHHH
VGPQGAVISYEIREDHAEHAVRNVTTFFGERPDNWDLVIGDLAERPADAGSVDRVVLDML
CCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHCCCCCCCCHHHHHHHHH
APWETLPAVAETLVPGGVLIVYVATVTQLSRVVEALREQQCWTEPRSWETMQRGWNVVGL
CCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEE
AVRPEHSMRGHTAFLVSARRLAPGTITPTPLRRKRLPS
EECCCCCCCCCEEEEEEHHHCCCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA