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The map label for this gene is nuoI [H]

Identifier: 169629228

GI number: 169629228

Start: 2154764

End: 2155300

Strand: Direct

Name: nuoI [H]

Synonym: MAB_2142

Alternate gene names: 169629228

Gene position: 2154764-2155300 (Clockwise)

Preceding gene: 169629227

Following gene: 169629229

Centisome position: 42.52

GC content: 62.38

Gene sequence:

>537_bases
ATGCCTGATCTTCTGCGACGTTCGGCCGATGCCATGGCCGGGTTCTGGGTCACGTTCTCGTCCATGTTCAAGAAGCGGCT
CACCGAGCAGTACCCCGAAAAGAAGCAGCCGACCGCACCCCGATATCACGGGCGGCACCAGCTCAATCGCTATGCCGACG
GCCTGGAGAAATGCATCGGTTGCGAGTTGTGCGCTTGGGCTTGTCCGGCCGACGCGATATTCGTCGAGGGCGCAGACAAT
TCCGAAGAGGAGCGGTTCTCACCGGGGGAACGGTACGGCCGGGTTTACCAGATCAACTATCTGCGGTGCATCGGTTGTGG
ACTGTGTATTGAGGCGTGTCCCACGCGGGCGCTGACCATGACCAACGACTACGAGATGGCCGACGACAACCGGGCCGATC
TCATCTACGGCAAGGACAAGCTGCTCGCGCCGCTGCAGCCGGGCATGACGGCGCCACCGCACGCGATGTACCCCGGGGCC
ACGGACACCGACTATTACCTGGGTAACCTCCCGAAGGCCGGGGAGGAAGTCAGGTGA

Upstream 100 bases:

>100_bases
CCCGCACGTTTCGATCCGATGGCCGGCGGATTCCCGGTGCCGCCGCTGCCGGGACAACGCGTCGACACCGGGGCGCACCC
GAGCACCAAGGAGGACGCAG

Downstream 100 bases:

>100_bases
GTCTTGGTTTTTGGGCCAGCCACGCGACAGTGCTTGCGGCCGAGGGGCTATCGCGCACCCCGACATGGGAAGGGGTGACG
TTTTGGGTGCTCGGCGCCAT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MPDLLRRSADAMAGFWVTFSSMFKKRLTEQYPEKKQPTAPRYHGRHQLNRYADGLEKCIGCELCAWACPADAIFVEGADN
SEEERFSPGERYGRVYQINYLRCIGCGLCIEACPTRALTMTNDYEMADDNRADLIYGKDKLLAPLQPGMTAPPHAMYPGA
TDTDYYLGNLPKAGEEVR

Sequences:

>Translated_178_residues
MPDLLRRSADAMAGFWVTFSSMFKKRLTEQYPEKKQPTAPRYHGRHQLNRYADGLEKCIGCELCAWACPADAIFVEGADN
SEEERFSPGERYGRVYQINYLRCIGCGLCIEACPTRALTMTNDYEMADDNRADLIYGKDKLLAPLQPGMTAPPHAMYPGA
TDTDYYLGNLPKAGEEVR
>Mature_177_residues
PDLLRRSADAMAGFWVTFSSMFKKRLTEQYPEKKQPTAPRYHGRHQLNRYADGLEKCIGCELCAWACPADAIFVEGADNS
EEERFSPGERYGRVYQINYLRCIGCGLCIEACPTRALTMTNDYEMADDNRADLIYGKDKLLAPLQPGMTAPPHAMYPGAT
DTDYYLGNLPKAGEEVR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=129, Percent_Identity=38.7596899224806, Blast_Score=102, Evalue=2e-22,
Organism=Escherichia coli, GI1788617, Length=142, Percent_Identity=40.8450704225352, Blast_Score=111, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17555194, Length=129, Percent_Identity=38.7596899224806, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17864306, Length=146, Percent_Identity=40.4109589041096, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20047; Mature: 19916

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLRRSADAMAGFWVTFSSMFKKRLTEQYPEKKQPTAPRYHGRHQLNRYADGLEKCIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
CELCAWACPADAIFVEGADNSEEERFSPGERYGRVYQINYLRCIGCGLCIEACPTRALTM
HHHHHHCCCCCEEEEECCCCCHHHHCCCHHHCCCEEEECHHHHHHHHHHHHHCCCCEEEE
TNDYEMADDNRADLIYGKDKLLAPLQPGMTAPPHAMYPGATDTDYYLGNLPKAGEEVR
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PDLLRRSADAMAGFWVTFSSMFKKRLTEQYPEKKQPTAPRYHGRHQLNRYADGLEKCIG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
CELCAWACPADAIFVEGADNSEEERFSPGERYGRVYQINYLRCIGCGLCIEACPTRALTM
HHHHHHCCCCCEEEEECCCCCHHHHCCCHHHCCCEEEECHHHHHHHHHHHHHCCCCEEEE
TNDYEMADDNRADLIYGKDKLLAPLQPGMTAPPHAMYPGATDTDYYLGNLPKAGEEVR
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA