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The map label for this gene is rsmE [H]

Identifier: 169628757

GI number: 169628757

Start: 1695494

End: 1696270

Strand: Direct

Name: rsmE [H]

Synonym: MAB_1667

Alternate gene names: 169628757

Gene position: 1695494-1696270 (Clockwise)

Preceding gene: 169628756

Following gene: 169628758

Centisome position: 33.46

GC content: 70.79

Gene sequence:

>777_bases
ATGGCGGCAGCCACGATCTTCTACGTTCCGCGGGTTCCCTCAGCCGGGGAGCAGGTGCTGCTCGACGGGGATGAGGGCCA
CCACGCGGCGCGGGTGTTGCGCATGCGAGTCGGCGAGCCTGTGATGGTGTGCGACGGTGCCGGTGAGGTCGGGGACGGCG
TCGTTGCGTCCGTTGACCGCACCGGCCTCGCAATCGAGGTGACCGGGCGCTGGAGTGTTGCCGCCGTTAACCCCAAAGTG
ACTGTGGTGCAGGCACTTCCTAAATCGGATCGCTCCGATCTGGCGGTCGATCTGGCGGTAGAGGCTGGTGCCGATGAAGT
GATCCCGTGGCAGGCGCAGCGGTGCGTGTCGCGCTGGGATGCGCACAAGGCAGAAAAGGGCCGGCGCCGCTGGGAAGCCG
TCGCCCAGGCAGCATCGCGGCAATCTCGCCGGGCCCACGTGCCATCGGTGCCCGAGCTGCACTCGACGGCGCAGCTGGCT
GAGCTGGTGGGTGCCCGCGCGGCGGGCGGAGCCGTCGTGCTGGTGCTGCACGAATCGGCGGAATCGGGTCTCGGCGCCCT
GGGAGCGCGGCTCTCGTCGGCGTCGTCGGTGGTGTTGATCGTGGGCCCCGAAGGCGGTGTCGCGGACGAGGAGCTGCGGA
TTTTGGCGGACGCCGGTGCCGCTCCGGTGCGGTTGGGGCCGACGGTGCTGCGTACCTCCAGTGCCGCCGCCGTCGCACTG
GGCGCGCTCGGGGTGCTGACGGATCGCTGGGCCGCCGGCCCGCTGGTGCACCGGTGA

Upstream 100 bases:

>100_bases
CTTCAGGGCTCGCAACAAGGAAGGTGCCGAGGTCGTGTGGGCCGAGTCGGCCTCCGGCGGGGTGTTCTCGCGGCTGCGCG
AGGCCTTCACGGGGCGGTAG

Downstream 100 bases:

>100_bases
GCGCTAATCGCGTGGAGGCCGACGGTTGCCGGCGGTAAACTCAAAGCAACTTCACAACCCAAGAAAGCAGGCGACCTTAA
CCACGTGACGAGCCGAGAAG

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAAATIFYVPRVPSAGEQVLLDGDEGHHAARVLRMRVGEPVMVCDGAGEVGDGVVASVDRTGLAIEVTGRWSVAAVNPKV
TVVQALPKSDRSDLAVDLAVEAGADEVIPWQAQRCVSRWDAHKAEKGRRRWEAVAQAASRQSRRAHVPSVPELHSTAQLA
ELVGARAAGGAVVLVLHESAESGLGALGARLSSASSVVLIVGPEGGVADEELRILADAGAAPVRLGPTVLRTSSAAAVAL
GALGVLTDRWAAGPLVHR

Sequences:

>Translated_258_residues
MAAATIFYVPRVPSAGEQVLLDGDEGHHAARVLRMRVGEPVMVCDGAGEVGDGVVASVDRTGLAIEVTGRWSVAAVNPKV
TVVQALPKSDRSDLAVDLAVEAGADEVIPWQAQRCVSRWDAHKAEKGRRRWEAVAQAASRQSRRAHVPSVPELHSTAQLA
ELVGARAAGGAVVLVLHESAESGLGALGARLSSASSVVLIVGPEGGVADEELRILADAGAAPVRLGPTVLRTSSAAAVAL
GALGVLTDRWAAGPLVHR
>Mature_257_residues
AAATIFYVPRVPSAGEQVLLDGDEGHHAARVLRMRVGEPVMVCDGAGEVGDGVVASVDRTGLAIEVTGRWSVAAVNPKVT
VVQALPKSDRSDLAVDLAVEAGADEVIPWQAQRCVSRWDAHKAEKGRRRWEAVAQAASRQSRRAHVPSVPELHSTAQLAE
LVGARAAGGAVVLVLHESAESGLGALGARLSSASSVVLIVGPEGGVADEELRILADAGAAPVRLGPTVLRTSSAAAVALG
ALGVLTDRWAAGPLVHR

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=241, Percent_Identity=26.5560165975104, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26586; Mature: 26454

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAATIFYVPRVPSAGEQVLLDGDEGHHAARVLRMRVGEPVMVCDGAGEVGDGVVASVDR
CCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECC
TGLAIEVTGRWSVAAVNPKVTVVQALPKSDRSDLAVDLAVEAGADEVIPWQAQRCVSRWD
CCEEEEEECCEEEEEECCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
AHKAEKGRRRWEAVAQAASRQSRRAHVPSVPELHSTAQLAELVGARAAGGAVVLVLHESA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCC
ESGLGALGARLSSASSVVLIVGPEGGVADEELRILADAGAAPVRLGPTVLRTSSAAAVAL
CCCHHHHHHHHCCCCCEEEEECCCCCCCHHHEEEEECCCCCCEEECCHHEECCCHHHHHH
GALGVLTDRWAAGPLVHR
HHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AAATIFYVPRVPSAGEQVLLDGDEGHHAARVLRMRVGEPVMVCDGAGEVGDGVVASVDR
CCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECC
TGLAIEVTGRWSVAAVNPKVTVVQALPKSDRSDLAVDLAVEAGADEVIPWQAQRCVSRWD
CCEEEEEECCEEEEEECCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
AHKAEKGRRRWEAVAQAASRQSRRAHVPSVPELHSTAQLAELVGARAAGGAVVLVLHESA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCC
ESGLGALGARLSSASSVVLIVGPEGGVADEELRILADAGAAPVRLGPTVLRTSSAAAVAL
CCCHHHHHHHHCCCCCEEEEECCCCCCCHHHEEEEECCCCCCEEECCHHEECCCHHHHHH
GALGVLTDRWAAGPLVHR
HHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]