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The map label for this gene is pimE [H]

Identifier: 169627386

GI number: 169627386

Start: 280352

End: 281611

Strand: Direct

Name: pimE [H]

Synonym: MAB_0281

Alternate gene names: 169627386

Gene position: 280352-281611 (Clockwise)

Preceding gene: 169627385

Following gene: 169627387

Centisome position: 5.53

GC content: 64.29

Gene sequence:

>1260_bases
GTGACGGTCCGCATTATGTCGTGGCGGACGGCCGGGTTCTGGCTGGCGGCCGGCCTTGTGGCTGTGCTGTTACAGCATTG
GCTGATACCGCTGAACGCCCCAAACAGCTTCGGGTTGTTCAGCAACGGTGGTGATCTGATGACCTACCGGTTCGGCGGGC
TGCGGGTACTGCACCGTGAACTGTTGTATGCCACAGAAATTCCCGACGCGGGATGGTTCACCTACACACCGTTCGCGGCG
ATGCTCTTCGCGCCATTGGGTTTTGTGACGTTGGGGGCGGCGAAGGTCATCTGGTTTCTGGTGAATCTGGGCGCGCTGTT
CATCATCATCTGGCGCTGCTGGCGGGTGCTGGGATTCTCCGCGAATGCTCCCCTGGCCATCGCGTGCGTCGGAATGAACT
TGGTGGCCTGGGATATTCAGCCGATTCACGGCACCCTGTGGCAGGGGCAGGTCAACCTGGTGCTGGCCGCGCTCATCATC
TGGGACCTGACTCGGCCGGAGGGCGCCCGCTGGCGCGGCTGGTCGGTGGGTCTGGCGTCGGGAGTCAAGCTGACAGCCGT
CATCTTTGTCCCCTACCTGTTGATCACCCGCCAATGGCGCGCCGTAGCGGCTGCGGCGGTCACGGCGATCGGCACGGTTG
TACTGGGATGGATTGTGCTGCCCTCCGATTCATCCGACTACTGGTTGGGCGCGGTACGCACCACCGGCCACATCGGCTCG
CTGGACCACCCGGCCAATTCTTCTATTGGCGGCGCTTTGGCGAATGTGTATACACCGCAACCGATGCCGACGTGGTTGTG
GGTGCTGCTCGCCGGTGGCGCGGCACTGCTGGGGATGGCGGCCGCGTGGCGGGCACACCGGACGGGCCGTGAGCTCCTGG
CCATCACCGTCGTTGGCATGGTCGGCTGCGTTGTGTCGCCGCTGGCATGGACCCACCACTGGGTGTGGACGGTGCCGCTG
ATGGTGTTGTTGGTCAACCAGATCATGACGACGCAGGGCGGGGCACGGTGGCGATGGGCCGCGGCCACCACCGTGATCGC
GGTACTCGTATCCATGTGGTGGTACTGGGGGCTGTACGTACGCGCGATGCGGCTCAACCCTGACTTCGAGTCGTACATCA
CCGGCTGGGGCGCCGTCATCGCGCACATGTCGCGTGCGGAGAGGGTCTTCGACACCGCCCTCTACCCGTTGCTGTTCCTT
ACGGTGGCGACCTGGGTCCTAGCGACCAAGTCCCTACGTGCCGGTACCGTGCAGGAATGA

Upstream 100 bases:

>100_bases
TGAACGGGCTGGGCGATATGCCCTATCCGCCGAACTACCCGAAGATGCCGGGAGAACCGAAACGCGTTCAGCCTTCCCGG
GATACCGATAGGAAGCAGTA

Downstream 100 bases:

>100_bases
GACGTTGGGCGGCGACCTGTGCCGTCGCACTGTTCACGGTGAGTGGTTGTTCGGTATGGGACAAGGCGGAGTCCACCCCG
ACGTCCTACACACCGCAACA

Product: hypothetical protein

Products: NA

Alternate protein names: Alpha-mannosyltransferase; Alpha-manT; PPM-dependent mannosyltransferase; Polyprenol-phosphate-mannose alpha-mannosyltransferase; PPM alpha-mannosyltransferase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MTVRIMSWRTAGFWLAAGLVAVLLQHWLIPLNAPNSFGLFSNGGDLMTYRFGGLRVLHRELLYATEIPDAGWFTYTPFAA
MLFAPLGFVTLGAAKVIWFLVNLGALFIIIWRCWRVLGFSANAPLAIACVGMNLVAWDIQPIHGTLWQGQVNLVLAALII
WDLTRPEGARWRGWSVGLASGVKLTAVIFVPYLLITRQWRAVAAAAVTAIGTVVLGWIVLPSDSSDYWLGAVRTTGHIGS
LDHPANSSIGGALANVYTPQPMPTWLWVLLAGGAALLGMAAAWRAHRTGRELLAITVVGMVGCVVSPLAWTHHWVWTVPL
MVLLVNQIMTTQGGARWRWAAATTVIAVLVSMWWYWGLYVRAMRLNPDFESYITGWGAVIAHMSRAERVFDTALYPLLFL
TVATWVLATKSLRAGTVQE

Sequences:

>Translated_419_residues
MTVRIMSWRTAGFWLAAGLVAVLLQHWLIPLNAPNSFGLFSNGGDLMTYRFGGLRVLHRELLYATEIPDAGWFTYTPFAA
MLFAPLGFVTLGAAKVIWFLVNLGALFIIIWRCWRVLGFSANAPLAIACVGMNLVAWDIQPIHGTLWQGQVNLVLAALII
WDLTRPEGARWRGWSVGLASGVKLTAVIFVPYLLITRQWRAVAAAAVTAIGTVVLGWIVLPSDSSDYWLGAVRTTGHIGS
LDHPANSSIGGALANVYTPQPMPTWLWVLLAGGAALLGMAAAWRAHRTGRELLAITVVGMVGCVVSPLAWTHHWVWTVPL
MVLLVNQIMTTQGGARWRWAAATTVIAVLVSMWWYWGLYVRAMRLNPDFESYITGWGAVIAHMSRAERVFDTALYPLLFL
TVATWVLATKSLRAGTVQE
>Mature_418_residues
TVRIMSWRTAGFWLAAGLVAVLLQHWLIPLNAPNSFGLFSNGGDLMTYRFGGLRVLHRELLYATEIPDAGWFTYTPFAAM
LFAPLGFVTLGAAKVIWFLVNLGALFIIIWRCWRVLGFSANAPLAIACVGMNLVAWDIQPIHGTLWQGQVNLVLAALIIW
DLTRPEGARWRGWSVGLASGVKLTAVIFVPYLLITRQWRAVAAAAVTAIGTVVLGWIVLPSDSSDYWLGAVRTTGHIGSL
DHPANSSIGGALANVYTPQPMPTWLWVLLAGGAALLGMAAAWRAHRTGRELLAITVVGMVGCVVSPLAWTHHWVWTVPLM
VLLVNQIMTTQGGARWRWAAATTVIAVLVSMWWYWGLYVRAMRLNPDFESYITGWGAVIAHMSRAERVFDTALYPLLFLT
VATWVLATKSLRAGTVQE

Specific function: Catalyzes the alpha-1,2 addition of a mannose residue from polyprenol-phosphate-mannose (PPM) to a monoacyl phosphatidylinositol pentamannoside (AcPIM5) to generate a monoacyl phosphatidylinositol hexamannoside (AcPIM6) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PIGM family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018584 [H]

Pfam domain/function: PF09594 DUF2029 [H]

EC number: NA

Molecular weight: Translated: 46076; Mature: 45945

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRIMSWRTAGFWLAAGLVAVLLQHWLIPLNAPNSFGLFSNGGDLMTYRFGGLRVLHRE
CEEEEEECCCHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHH
LLYATEIPDAGWFTYTPFAAMLFAPLGFVTLGAAKVIWFLVNLGALFIIIWRCWRVLGFS
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ANAPLAIACVGMNLVAWDIQPIHGTLWQGQVNLVLAALIIWDLTRPEGARWRGWSVGLAS
CCCCEEEEEECCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEECCCCC
GVKLTAVIFVPYLLITRQWRAVAAAAVTAIGTVVLGWIVLPSDSSDYWLGAVRTTGHIGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECCCCCC
LDHPANSSIGGALANVYTPQPMPTWLWVLLAGGAALLGMAAAWRAHRTGRELLAITVVGM
CCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
VGCVVSPLAWTHHWVWTVPLMVLLVNQIMTTQGGARWRWAAATTVIAVLVSMWWYWGLYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
RAMRLNPDFESYITGWGAVIAHMSRAERVFDTALYPLLFLTVATWVLATKSLRAGTVQE
HHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVRIMSWRTAGFWLAAGLVAVLLQHWLIPLNAPNSFGLFSNGGDLMTYRFGGLRVLHRE
EEEEEECCCHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHH
LLYATEIPDAGWFTYTPFAAMLFAPLGFVTLGAAKVIWFLVNLGALFIIIWRCWRVLGFS
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ANAPLAIACVGMNLVAWDIQPIHGTLWQGQVNLVLAALIIWDLTRPEGARWRGWSVGLAS
CCCCEEEEEECCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEECCCCC
GVKLTAVIFVPYLLITRQWRAVAAAAVTAIGTVVLGWIVLPSDSSDYWLGAVRTTGHIGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECCCCCC
LDHPANSSIGGALANVYTPQPMPTWLWVLLAGGAALLGMAAAWRAHRTGRELLAITVVGM
CCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
VGCVVSPLAWTHHWVWTVPLMVLLVNQIMTTQGGARWRWAAATTVIAVLVSMWWYWGLYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
RAMRLNPDFESYITGWGAVIAHMSRAERVFDTALYPLLFLTVATWVLATKSLRAGTVQE
HHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]