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The map label for this gene is rfaP

Identifier: 16767006

GI number: 16767006

Start: 3915948

End: 3916745

Strand: Reverse

Name: rfaP

Synonym: STM3721

Alternate gene names: 16767006

Gene position: 3916745-3915948 (Counterclockwise)

Preceding gene: 16767007

Following gene: 16767005

Centisome position: 80.63

GC content: 46.12

Gene sequence:

>798_bases
ATGGTTGAGCTGAAAGCGCCGTTAACCACACTATGGCGCGGTAAAGATGCTTTTGAGGAAGTAAAAACGTTACAGGGCGA
AGTGTTCAGAGAGCTGGAGACGCGTCGAACATTGCGGTTTGAGCTGGACGGTAAAAGCTACTTCCTGAAGTGGCATAAAG
GTACTTCGCTGAAAGAAATTGTGAAGAACCTGATTTCGTTACGTATGCCTGTTCTGGGCGCTGACAGAGAATGGCACGCC
ATTCATCGTCTACATGAGCTGGGCGTTGATACGATGCACGGCGTTGGTTTTGGTGAAAAAGGCGTAAACCCGCTAACCAG
AACATCATTTATTATCACCGAAGATTTAACGCCCACCATTAGCCTTGAAGACTACTGTGCTGACTGGGCTGTTAATCCAC
CGGACGCGCAGGTGAAGTGGATGATCATTAAGCGTGTTGCGACTATGGTACGTAAAATGCACGCCGGGGGAATTAACCAT
CGCGACTGTTATATTTGCCACTTTCTTCTGCATTTACCTTTCACTGGTCGCGAAGAGGATTTAAAAATCTCCGTAATTGA
CCTGCATCGCGCGCAGATACGCCAGCACGTTCCCCTTCGCTGGCGTGACAAAGATTTAATTGGGCTTTATTTTTCTTCAA
TGAATATTGGCCTGACTCAGCGAGATATATTCCGGTTTATGCGTGAGTATTTCTCTCTCCCCCTGCGAGAGATTTTGCAA
AAAGAATCGGGGTTGATTCATCAGGCAGATGTTAAAGCCGCTCGAATTAAAGAAAGGACAATAAGAAAAAATCTGTAA

Upstream 100 bases:

>100_bases
TTACGCAACGCCTGGGCTGAAAATGCGCGGTATTATGCTGATACCCAGGATTTATACAGCTTACCGGAGAAGGCCGCGGA
TATTATTACAGGTGATTTAG

Downstream 100 bases:

>100_bases
TTATAATAATTATGCACTCAGTGAGTAATTTATGAGTGAGACAAGGATAGACAGAATGTCTAACATTAAAAGTTATATTT
CAAACCAAAAAAACATAATT

Product: lipopolysaccharide core biosynthetic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MVELKAPLTTLWRGKDAFEEVKTLQGEVFRELETRRTLRFELDGKSYFLKWHKGTSLKEIVKNLISLRMPVLGADREWHA
IHRLHELGVDTMHGVGFGEKGVNPLTRTSFIITEDLTPTISLEDYCADWAVNPPDAQVKWMIIKRVATMVRKMHAGGINH
RDCYICHFLLHLPFTGREEDLKISVIDLHRAQIRQHVPLRWRDKDLIGLYFSSMNIGLTQRDIFRFMREYFSLPLREILQ
KESGLIHQADVKAARIKERTIRKNL

Sequences:

>Translated_265_residues
MVELKAPLTTLWRGKDAFEEVKTLQGEVFRELETRRTLRFELDGKSYFLKWHKGTSLKEIVKNLISLRMPVLGADREWHA
IHRLHELGVDTMHGVGFGEKGVNPLTRTSFIITEDLTPTISLEDYCADWAVNPPDAQVKWMIIKRVATMVRKMHAGGINH
RDCYICHFLLHLPFTGREEDLKISVIDLHRAQIRQHVPLRWRDKDLIGLYFSSMNIGLTQRDIFRFMREYFSLPLREILQ
KESGLIHQADVKAARIKERTIRKNL
>Mature_265_residues
MVELKAPLTTLWRGKDAFEEVKTLQGEVFRELETRRTLRFELDGKSYFLKWHKGTSLKEIVKNLISLRMPVLGADREWHA
IHRLHELGVDTMHGVGFGEKGVNPLTRTSFIITEDLTPTISLEDYCADWAVNPPDAQVKWMIIKRVATMVRKMHAGGINH
RDCYICHFLLHLPFTGREEDLKISVIDLHRAQIRQHVPLRWRDKDLIGLYFSSMNIGLTQRDIFRFMREYFSLPLREILQ
KESGLIHQADVKAARIKERTIRKNL

Specific function: Catalyzes the phosphorylation of heptose(I) of the outer membrane lipopolysaccharide core

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protein kinase superfamily. KdkA/rfaP family

Homologues:

Organism=Escherichia coli, GI1790060, Length=265, Percent_Identity=83.0188679245283, Blast_Score=461, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFAP_SALTY (Q06995)

Other databases:

- EMBL:   AF026386
- EMBL:   AE006468
- EMBL:   S56361
- PIR:   A47074
- RefSeq:   NP_462621.1
- PRIDE:   Q06995
- GeneID:   1255245
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM3721
- HOGENOM:   HBG336985
- OMA:   VPTMTAV
- ProtClustDB:   PRK15123
- BioCyc:   STYP99287:STM3721-MONOMER
- GO:   GO:0016020
- InterPro:   IPR011009
- InterPro:   IPR017172
- InterPro:   IPR010440
- PIRSF:   PIRSF037318

Pfam domain/function: PF06293 Kdo; SSF56112 Kinase_like

EC number: 2.7.-.-

Molecular weight: Translated: 30997; Mature: 30997

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: ACT_SITE 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVELKAPLTTLWRGKDAFEEVKTLQGEVFRELETRRTLRFELDGKSYFLKWHKGTSLKEI
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCCHHHH
VKNLISLRMPVLGADREWHAIHRLHELGVDTMHGVGFGEKGVNPLTRTSFIITEDLTPTI
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHCEEEEEECCCCCE
SLEDYCADWAVNPPDAQVKWMIIKRVATMVRKMHAGGINHRDCYICHFLLHLPFTGREED
EHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
LKISVIDLHRAQIRQHVPLRWRDKDLIGLYFSSMNIGLTQRDIFRFMREYFSLPLREILQ
CEEEEEEHHHHHHHHCCCCEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHH
KESGLIHQADVKAARIKERTIRKNL
HHCCCEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVELKAPLTTLWRGKDAFEEVKTLQGEVFRELETRRTLRFELDGKSYFLKWHKGTSLKEI
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCCHHHH
VKNLISLRMPVLGADREWHAIHRLHELGVDTMHGVGFGEKGVNPLTRTSFIITEDLTPTI
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHCEEEEEECCCCCE
SLEDYCADWAVNPPDAQVKWMIIKRVATMVRKMHAGGINHRDCYICHFLLHLPFTGREED
EHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
LKISVIDLHRAQIRQHVPLRWRDKDLIGLYFSSMNIGLTQRDIFRFMREYFSLPLREILQ
CEEEEEEHHHHHHHHCCCCEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHH
KESGLIHQADVKAARIKERTIRKNL
HHCCCEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9535865; 11677609; 8444813