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The map label for this gene is ygbJ [H]

Identifier: 16766224

GI number: 16766224

Start: 3060704

End: 3061627

Strand: Reverse

Name: ygbJ [H]

Synonym: STM2918

Alternate gene names: 16766224

Gene position: 3061627-3060704 (Counterclockwise)

Preceding gene: 16766226

Following gene: 16766223

Centisome position: 63.03

GC content: 59.96

Gene sequence:

>924_bases
ATGACAACAGGAACCGATTTTCATGTCGGCATTGTGGGGCTTGGCTCAATGGGAATGGGCGCTGCGCGTTCATGCCTGCG
CGCCGGGCTTTCCACATGGGGGGCCGATCTTAACCCGCAAGCGTGCGCGAATCTGCTCGCTGAAGGGGCTTGTGGGGCTG
CCGCCAGCGCCAGGGAGTTCGCCGGCGTCGTGGATGCGTTAGTCATTCTGGTCGTCAATGCCGCCCAGGTCAGACAGGTG
TTATTCGGTGAGGATGGCGTCGCCCATCTGATGAAACCCGGAAGCGCGGTGATGGTTTCGTCCACGATCTCAAGCGCCGA
CGCGCAAGAGATCGCCGCGGCGTTGACGGCATTGAATCTTAACATGCTGGATGCGCCGGTTTCCGGCGGTGCAGTAAAAG
CGGCGCAGGGCGAAATGACGGTGATGGCCTCCGGGAGCGAGGCGGCGTTTACCCGCCTGAAGCCTGTGCTGGACGCCGTT
GCCAGCAACGTTTATCGCATCAGCGATACGCCGGGCGCAGGCTCAACCGTCAAAATCATTCATCAACTGCTGGCTGGCGT
GCATATTGCGGCGGCGGCCGAAGCGATGGCGCTGGCCGCGCGGGCCGGTATTCCTCTGGACGTGATGTATGACGTCGTGA
CCCATGCCGCCGGCAACTCCTGGATGTTTGAAAACCGTATGCAGCATGTCGTTGACGGCGACTATACCCCGCGTTCGGCC
GTCGACATTTTTGTCAAAGATTTGGGGCTGGTTGCCGACACGGCGAAAGCGCTGCGCTTCCCCTTACCGCTGGCCTCTAC
GGCGTTAAATATGTTCACCAGCGCCAGTAACGCCGGTTATGGCAAAGAGGATGACAGCGCTGTGATCAAAATTTTCTCGG
GGATTACTCTGCCTGGCGTCACGCCTGAGGAGCCGTCATGTTAA

Upstream 100 bases:

>100_bases
TTTTGTTATTTTTTGTGAAACAACTCACCGCAATTGTTTACAAAAACGCCTATATTCACATAACAAAACATAATATCACG
TAAATTAACATGGAGTGATA

Downstream 100 bases:

>100_bases
AAATTGGCGTCATTGCCGATGATTTTACCGGCGCGACCGACATCGCCAGTTTTCTGGTCGAAAACGGGATGCCGACAGTG
CAGATCAATGATGTCCCAAC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTTGTDFHVGIVGLGSMGMGAARSCLRAGLSTWGADLNPQACANLLAEGACGAAASAREFAGVVDALVILVVNAAQVRQV
LFGEDGVAHLMKPGSAVMVSSTISSADAQEIAAALTALNLNMLDAPVSGGAVKAAQGEMTVMASGSEAAFTRLKPVLDAV
ASNVYRISDTPGAGSTVKIIHQLLAGVHIAAAAEAMALAARAGIPLDVMYDVVTHAAGNSWMFENRMQHVVDGDYTPRSA
VDIFVKDLGLVADTAKALRFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGVTPEEPSC

Sequences:

>Translated_307_residues
MTTGTDFHVGIVGLGSMGMGAARSCLRAGLSTWGADLNPQACANLLAEGACGAAASAREFAGVVDALVILVVNAAQVRQV
LFGEDGVAHLMKPGSAVMVSSTISSADAQEIAAALTALNLNMLDAPVSGGAVKAAQGEMTVMASGSEAAFTRLKPVLDAV
ASNVYRISDTPGAGSTVKIIHQLLAGVHIAAAAEAMALAARAGIPLDVMYDVVTHAAGNSWMFENRMQHVVDGDYTPRSA
VDIFVKDLGLVADTAKALRFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGVTPEEPSC
>Mature_306_residues
TTGTDFHVGIVGLGSMGMGAARSCLRAGLSTWGADLNPQACANLLAEGACGAAASAREFAGVVDALVILVVNAAQVRQVL
FGEDGVAHLMKPGSAVMVSSTISSADAQEIAAALTALNLNMLDAPVSGGAVKAAQGEMTVMASGSEAAFTRLKPVLDAVA
SNVYRISDTPGAGSTVKIIHQLLAGVHIAAAAEAMALAARAGIPLDVMYDVVTHAAGNSWMFENRMQHVVDGDYTPRSAV
DIFVKDLGLVADTAKALRFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGVTPEEPSC

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=28.5223367697594, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI40556376, Length=290, Percent_Identity=23.1034482758621, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1789092, Length=299, Percent_Identity=82.2742474916388, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI145693186, Length=282, Percent_Identity=33.6879432624113, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786719, Length=282, Percent_Identity=30.1418439716312, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1790315, Length=292, Percent_Identity=30.4794520547945, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17557316, Length=271, Percent_Identity=28.4132841328413, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24655230, Length=298, Percent_Identity=28.8590604026846, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922568, Length=298, Percent_Identity=28.8590604026846, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28574115, Length=283, Percent_Identity=25.0883392226148, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31262; Mature: 31131

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGTDFHVGIVGLGSMGMGAARSCLRAGLSTWGADLNPQACANLLAEGACGAAASAREF
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHH
AGVVDALVILVVNAAQVRQVLFGEDGVAHLMKPGSAVMVSSTISSADAQEIAAALTALNL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECHHCCCCHHHHHHHHHHHCC
NMLDAPVSGGAVKAAQGEMTVMASGSEAAFTRLKPVLDAVASNVYRISDTPGAGSTVKII
HHCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHH
HQLLAGVHIAAAAEAMALAARAGIPLDVMYDVVTHAAGNSWMFENRMQHVVDGDYTPRSA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHH
VDIFVKDLGLVADTAKALRFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCC
TPEEPSC
CCCCCCC
>Mature Secondary Structure 
TTGTDFHVGIVGLGSMGMGAARSCLRAGLSTWGADLNPQACANLLAEGACGAAASAREF
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHH
AGVVDALVILVVNAAQVRQVLFGEDGVAHLMKPGSAVMVSSTISSADAQEIAAALTALNL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECHHCCCCHHHHHHHHHHHCC
NMLDAPVSGGAVKAAQGEMTVMASGSEAAFTRLKPVLDAVASNVYRISDTPGAGSTVKII
HHCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHH
HQLLAGVHIAAAAEAMALAARAGIPLDVMYDVVTHAAGNSWMFENRMQHVVDGDYTPRSA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHH
VDIFVKDLGLVADTAKALRFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCC
TPEEPSC
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]