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The map label for this gene is rffG [C]

Identifier: 16766220

GI number: 16766220

Start: 3057042

End: 3057992

Strand: Reverse

Name: rffG [C]

Synonym: STM2914

Alternate gene names: 16766220

Gene position: 3057992-3057042 (Counterclockwise)

Preceding gene: 16766221

Following gene: 16766219

Centisome position: 62.95

GC content: 56.15

Gene sequence:

>951_bases
ATGCAGATTATCATTACCGGCGGGGGCGGCTTTTTAGGCCAGAAACTCGCAAGCGCCTTATTAAACTCATCGCTGGCGTT
TAACGAACTGCTTCTTGTTGATTTAAAAATGCCTGCACGGTTATCAGATTCCCCTCGTTTACGCTGCCTGGAGGCTGACT
TAACCCAGCCGGGCGTGCTGGAGAGTGTGATTACCGCTAATACCTCTGTTGTTTATCATCTCGCTGCGATTGTCAGCAGT
CATGCGGAAGACGATTTCGATCTGGGATGGAAAGTTAACCTGGATCTTACCCGCCAGTTACTTGAGGCGTGTCGTCGACA
ACCGCAGAAAATTCGTTTTGTCTTCTCCAGCTCGCTTGCCGTTTATGGCGGTACGCTGCCGGAATGCGTCACCGATACCA
CCGCGCTCACGCCGCGCTCGTCTTATGGCGCGCAGAAGGCCGCCTGTGAACTGTTGGTCAACGACTATACCCGCAAAGGC
TATGTGGATGGGCTGGCGCTGCGTTTGCCGACGATCTGTGTTCGCCCGGGTAAACCAAACCGCGCCGCTTCTTCTTTTGT
CAGCGCGATTATTCGTGAACCGTTGCAGGGCGAGACGACCGTCTGCCCGGTGTCGGAAAGTTTGCGGCTGTGGATTTCCA
GCCCGGCGACGGTGATCCATAACCTGTCGCTGGCCGCAACGTTACCCGCGCCTGGCGAGGCGAGCAGCATCAACTTACCC
GGGATCAGCGTAACCGTGGGCGAGATGCTGGAAACGTTGTGTCAGGCGGGCGGCCAGGCGGCGCGCGATCGGGTTACGCA
TCAGCGCGACGAAGGCGTCGAGAAAATTGTTGCCTCCTGGCCGGGACGTATCGATAACCAGCGTGCGCTGGCGTTAGGTT
TTGTCGCCGATAAACGCTTCGATGACATTATCGAACGCTTTCGACAAGATGATATGGAGGGGAGGTCATGA

Upstream 100 bases:

>100_bases
CGAATACCAACCACGGAATACCACTCAGGACGGGCTTGGCTGGTTTAACGCCTGGCGCTAACCCTGAACTACCCTACACA
GCAATAAAGAGGACATCCGG

Downstream 100 bases:

>100_bases
CAAGTTTACCGACCCCAATTATCGGTCTGATCGTTGCCGTTTTCGTGCTGGTATGGCTGGTGTTACGCACCCGTGTTCAT
GCGCTGATCGCGATGCTGGC

Product: nucleoside-diphosphate-sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MQIIITGGGGFLGQKLASALLNSSLAFNELLLVDLKMPARLSDSPRLRCLEADLTQPGVLESVITANTSVVYHLAAIVSS
HAEDDFDLGWKVNLDLTRQLLEACRRQPQKIRFVFSSSLAVYGGTLPECVTDTTALTPRSSYGAQKAACELLVNDYTRKG
YVDGLALRLPTICVRPGKPNRAASSFVSAIIREPLQGETTVCPVSESLRLWISSPATVIHNLSLAATLPAPGEASSINLP
GISVTVGEMLETLCQAGGQAARDRVTHQRDEGVEKIVASWPGRIDNQRALALGFVADKRFDDIIERFRQDDMEGRS

Sequences:

>Translated_316_residues
MQIIITGGGGFLGQKLASALLNSSLAFNELLLVDLKMPARLSDSPRLRCLEADLTQPGVLESVITANTSVVYHLAAIVSS
HAEDDFDLGWKVNLDLTRQLLEACRRQPQKIRFVFSSSLAVYGGTLPECVTDTTALTPRSSYGAQKAACELLVNDYTRKG
YVDGLALRLPTICVRPGKPNRAASSFVSAIIREPLQGETTVCPVSESLRLWISSPATVIHNLSLAATLPAPGEASSINLP
GISVTVGEMLETLCQAGGQAARDRVTHQRDEGVEKIVASWPGRIDNQRALALGFVADKRFDDIIERFRQDDMEGRS
>Mature_316_residues
MQIIITGGGGFLGQKLASALLNSSLAFNELLLVDLKMPARLSDSPRLRCLEADLTQPGVLESVITANTSVVYHLAAIVSS
HAEDDFDLGWKVNLDLTRQLLEACRRQPQKIRFVFSSSLAVYGGTLPECVTDTTALTPRSSYGAQKAACELLVNDYTRKG
YVDGLALRLPTICVRPGKPNRAASSFVSAIIREPLQGETTVCPVSESLRLWISSPATVIHNLSLAATLPAPGEASSINLP
GISVTVGEMLETLCQAGGQAARDRVTHQRDEGVEKIVASWPGRIDNQRALALGFVADKRFDDIIERFRQDDMEGRS

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI32566934, Length=294, Percent_Identity=23.1292517006803, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24667531, Length=293, Percent_Identity=24.2320819112628, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 34176; Mature: 34176

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIIITGGGGFLGQKLASALLNSSLAFNELLLVDLKMPARLSDSPRLRCLEADLTQPGVL
CEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCHHH
ESVITANTSVVYHLAAIVSSHAEDDFDLGWKVNLDLTRQLLEACRRQPQKIRFVFSSSLA
HHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHCCHHHEEEHHCCCHH
VYGGTLPECVTDTTALTPRSSYGAQKAACELLVNDYTRKGYVDGLALRLPTICVRPGKPN
EECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEECCCCCC
RAASSFVSAIIREPLQGETTVCPVSESLRLWISSPATVIHNLSLAATLPAPGEASSINLP
HHHHHHHHHHHHCCCCCCCEEECCCHHHEEEECCCHHHHHCCEEEEECCCCCCCCEEECC
GISVTVGEMLETLCQAGGQAARDRVTHQRDEGVEKIVASWPGRIDNQRALALGFVADKRF
CCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHH
DDIIERFRQDDMEGRS
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQIIITGGGGFLGQKLASALLNSSLAFNELLLVDLKMPARLSDSPRLRCLEADLTQPGVL
CEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCHHH
ESVITANTSVVYHLAAIVSSHAEDDFDLGWKVNLDLTRQLLEACRRQPQKIRFVFSSSLA
HHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHCCHHHEEEHHCCCHH
VYGGTLPECVTDTTALTPRSSYGAQKAACELLVNDYTRKGYVDGLALRLPTICVRPGKPN
EECCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEECCCCCC
RAASSFVSAIIREPLQGETTVCPVSESLRLWISSPATVIHNLSLAATLPAPGEASSINLP
HHHHHHHHHHHHCCCCCCCEEECCCHHHEEEECCCHHHHHCCEEEEECCCCCCCCEEECC
GISVTVGEMLETLCQAGGQAARDRVTHQRDEGVEKIVASWPGRIDNQRALALGFVADKRF
CCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHH
DDIIERFRQDDMEGRS
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]