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The map label for this gene is spaS

Identifier: 16766193

GI number: 16766193

Start: 3031533

End: 3032603

Strand: Reverse

Name: spaS

Synonym: STM2887

Alternate gene names: 16766193

Gene position: 3032603-3031533 (Counterclockwise)

Preceding gene: 16766194

Following gene: 16766192

Centisome position: 62.43

GC content: 43.32

Gene sequence:

>1071_bases
ATGTCCTCGAATAAAACAGAAAAACCGACTAAAAAACGGCTGGAAGACTCCGCTAAAAAAGGCCAGTCATTTAAAAGTAA
AGATCTCATTATCGCCTGCCTGACGCTGGGAGGAATTGCCTATCTGGTGTCGTATGGCTCATTTAATGAGTTTATGGGGA
TAATTAAGATCATTATTGCGGATAATTTTGATCAGAGCATGGCTGACTACAGTTTGGCCGTTTTTGGGATAGGGTTAAAA
TATCTGATTCCATTTATGCTGCTCTGCTTAGTGTGTTCCGCATTACCGGCGTTATTACAGGCCGGTTTTGTGCTGGCGAC
AGAAGCATTAAAGCCTAATTTATCGGCGTTAAACCCGGTAGAAGGGGCAAAAAAACTTTTTAGTATGCGCACGGTTAAAG
ATACGGTCAAAACCCTACTGTATCTCTCATCCTTTGTGGTGGCCGCCATCATTTGCTGGAAGAAATATAAGGTTGAAATC
TTTTCTCAGCTAAATGGCAATATTGTAGGTATTGCCGTCATTTGGCGTGAACTTCTCCTCGCATTGGTATTAACTTGCCT
TGCTTGCGCATTGATTGTCTTATTATTGGATGCTATTGCGGAATATTTCCTGACCATGAAAGATATGAAAATGGATAAGG
AAGAAGTGAAGCGTGAAATGAAGGAGCAGGAAGGGAACCCAGAGGTTAAATCTAAAAGACGTGAAGTTCATATGGAAATT
CTGTCTGAACAGGTGAAATCTGATATTGAAAACTCACGCCTGATTGTTGCCAACCCCACGCATATTACGATCGGGATTTA
TTTTAAACCCGAATTGATGCCGATTCCGATGATCTCGGTGTATGAAACGAATCAGCGCGCACTGGCCGTCCGCGCCTATG
CGGAGAAGGTTGGCGTACCTGTGATCGTCGATATCAAACTGGCGCGCAGTCTTTTCAAAACCCATCGCCGTTATGATCTG
GTGAGTCTGGAAGAAATTGATGAAGTTTTACGTCTTCTGGTTTGGCTGGAAGAGGTAGAAAACGCGGGCAAAGACGTTAT
TCAGCCACAAGAAAACGAGGTACGGCATTGA

Upstream 100 bases:

>100_bases
ATGCTGCTTTATTTCTCTCCGGTACTACCGGACAATGTACTGCGACTCTCTTTCCAGGCCACAGGGTTAAGCAGTTGGTT
TTACGAGCGAGGGGCGACGC

Downstream 100 bases:

>100_bases
GCCGCGTAAGGCAGTAGCGATGTATTCATTGGGCGTTTTTTGAATGTTCACTAACCACCGTCGGGGTTTAATAACTGCAT
CAGATAAACGCAGTCGTTAA

Product: surface presentation of antigens protein SpaS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSSNKTEKPTKKRLEDSAKKGQSFKSKDLIIACLTLGGIAYLVSYGSFNEFMGIIKIIIADNFDQSMADYSLAVFGIGLK
YLIPFMLLCLVCSALPALLQAGFVLATEALKPNLSALNPVEGAKKLFSMRTVKDTVKTLLYLSSFVVAAIICWKKYKVEI
FSQLNGNIVGIAVIWRELLLALVLTCLACALIVLLLDAIAEYFLTMKDMKMDKEEVKREMKEQEGNPEVKSKRREVHMEI
LSEQVKSDIENSRLIVANPTHITIGIYFKPELMPIPMISVYETNQRALAVRAYAEKVGVPVIVDIKLARSLFKTHRRYDL
VSLEEIDEVLRLLVWLEEVENAGKDVIQPQENEVRH

Sequences:

>Translated_356_residues
MSSNKTEKPTKKRLEDSAKKGQSFKSKDLIIACLTLGGIAYLVSYGSFNEFMGIIKIIIADNFDQSMADYSLAVFGIGLK
YLIPFMLLCLVCSALPALLQAGFVLATEALKPNLSALNPVEGAKKLFSMRTVKDTVKTLLYLSSFVVAAIICWKKYKVEI
FSQLNGNIVGIAVIWRELLLALVLTCLACALIVLLLDAIAEYFLTMKDMKMDKEEVKREMKEQEGNPEVKSKRREVHMEI
LSEQVKSDIENSRLIVANPTHITIGIYFKPELMPIPMISVYETNQRALAVRAYAEKVGVPVIVDIKLARSLFKTHRRYDL
VSLEEIDEVLRLLVWLEEVENAGKDVIQPQENEVRH
>Mature_355_residues
SSNKTEKPTKKRLEDSAKKGQSFKSKDLIIACLTLGGIAYLVSYGSFNEFMGIIKIIIADNFDQSMADYSLAVFGIGLKY
LIPFMLLCLVCSALPALLQAGFVLATEALKPNLSALNPVEGAKKLFSMRTVKDTVKTLLYLSSFVVAAIICWKKYKVEIF
SQLNGNIVGIAVIWRELLLALVLTCLACALIVLLLDAIAEYFLTMKDMKMDKEEVKREMKEQEGNPEVKSKRREVHMEIL
SEQVKSDIENSRLIVANPTHITIGIYFKPELMPIPMISVYETNQRALAVRAYAEKVGVPVIVDIKLARSLFKTHRRYDLV
SLEEIDEVLRLLVWLEEVENAGKDVIQPQENEVRH

Specific function: Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells

COG id: COG1377

COG function: function code NU; Flagellar biosynthesis pathway, component FlhB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family

Homologues:

Organism=Escherichia coli, GI1788188, Length=371, Percent_Identity=23.4501347708895, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): SPAS_SALTY (P40702)

Other databases:

- EMBL:   X73525
- EMBL:   AE006468
- PIR:   S37356
- RefSeq:   NP_461808.1
- PDB:   3C01
- PDBsum:   3C01
- ProteinModelPortal:   P40702
- SMR:   P40702
- MEROPS:   N06.002
- PRIDE:   P40702
- GeneID:   1254410
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2887
- HOGENOM:   HBG529770
- OMA:   QPNPGAY
- ProtClustDB:   PRK08156
- BioCyc:   STYP99287:STM2887-MONOMER
- GO:   GO:0009405
- InterPro:   IPR006135
- InterPro:   IPR006307
- PRINTS:   PR00950
- TIGRFAMs:   TIGR01404

Pfam domain/function: PF01312 Bac_export_2

EC number: NA

Molecular weight: Translated: 40094; Mature: 39963

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd359eec)-; HASH(0x112eb548)-; HASH(0x10a7b230)-; HASH(0x10135260)-; HASH(0x112d8158)-;

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNKTEKPTKKRLEDSAKKGQSFKSKDLIIACLTLGGIAYLVSYGSFNEFMGIIKIIIA
CCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DNFDQSMADYSLAVFGIGLKYLIPFMLLCLVCSALPALLQAGFVLATEALKPNLSALNPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHH
EGAKKLFSMRTVKDTVKTLLYLSSFVVAAIICWKKYKVEIFSQLNGNIVGIAVIWRELLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
ALVLTCLACALIVLLLDAIAEYFLTMKDMKMDKEEVKREMKEQEGNPEVKSKRREVHMEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LSEQVKSDIENSRLIVANPTHITIGIYFKPELMPIPMISVYETNQRALAVRAYAEKVGVP
HHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCC
VIVDIKLARSLFKTHRRYDLVSLEEIDEVLRLLVWLEEVENAGKDVIQPQENEVRH
EEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
>Mature Secondary Structure 
SSNKTEKPTKKRLEDSAKKGQSFKSKDLIIACLTLGGIAYLVSYGSFNEFMGIIKIIIA
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DNFDQSMADYSLAVFGIGLKYLIPFMLLCLVCSALPALLQAGFVLATEALKPNLSALNPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHH
EGAKKLFSMRTVKDTVKTLLYLSSFVVAAIICWKKYKVEIFSQLNGNIVGIAVIWRELLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
ALVLTCLACALIVLLLDAIAEYFLTMKDMKMDKEEVKREMKEQEGNPEVKSKRREVHMEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LSEQVKSDIENSRLIVANPTHITIGIYFKPELMPIPMISVYETNQRALAVRAYAEKVGVP
HHHHHHHHHCCCEEEEECCCEEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCC
VIVDIKLARSLFKTHRRYDLVSLEEIDEVLRLLVWLEEVENAGKDVIQPQENEVRH
EEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8404849; 11677609