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The map label for this gene is dam [C]

Identifier: 16766042

GI number: 16766042

Start: 2872688

End: 2873545

Strand: Reverse

Name: dam [C]

Synonym: STM2730

Alternate gene names: 16766042

Gene position: 2873545-2872688 (Counterclockwise)

Preceding gene: 16766043

Following gene: 16766041

Centisome position: 59.16

GC content: 46.15

Gene sequence:

>858_bases
ATGAGCACCATCCTGAAATGGGCGGGTAATAAAACCGCCATCATGGCAGAACTGAAAAAACACCTCCCAGAAGGCCCGCG
ACTGGTTGAACCTTTCGCGGGTTCATGCGCTGTGATGATAGAGACAGACTATCCTCATTATCTTGTCGCGGATATTAATC
CAGACCTGATTAATCTCTATCAGGTGATTAAGAATGATGTTGAATACTTCATCAAAGAGGGCAGATATCTTTTTGAAGCC
CGTAATGATCCAGAGGCATATTATAAGACGAGACAGGAGTTTAACTTGCGCCATGGTGGCGCAATTGAACGGGCATTGTA
TTTCTTATATTTAAATCGCCATGGTTATCGCGGACTGTGTCGCTATAACTTGGACGGTTATTTTAATGTTCCTTACGGTA
ATTATAAAAAGCCGTACTTCCCTGAAAACGAAATACGTGCATTTGCAGAAAAAGCAAAACGCGCAACGTTTATCTGCGCC
AGCTATGACGAGACACTGGCACTGCTGCAAACGGGTGATGTTGTCTATTGCGATCCACCATATGACGGCACGTTTAACGG
ATATCACACAGCTGGTTTTACAGAGAATGATCAGTTCCATCTGGCGTCTATTCTTGAACGCCGGTCATCAGAAGGTCATC
CGGTTATCGTGTCCAACAGCGATACGTCTCTGACCCGTTCGCTTTATCGTGATTTTACTCGCCATCGTATAACCGCTAAG
CGCAGCATGGGCGTGGCTGCCGGTGATAGTAAAACTGCAGTAGAAATCATCGCCACAAAATCAGCATGCTGGTTTGGTGT
TGATTTGGCGTCTGGTCCTGATATCTCGGTAGAAACTGAGGTGCGGGCGTGGCAGTGA

Upstream 100 bases:

>100_bases
TCCCGCCAGCACGCCGCCGCGCCATTCCGGGCGTGCAGTGCTGCATCACCTGTCAGGAAATCGCAGAGCTGAAAGGCAAA
CACTACAACGGAGGTGCTGT

Downstream 100 bases:

>100_bases
GTAAATTCACATTACATAATGCACCAACCACCGGCGGTTCGAATGAGGCCGCCGTGGTCTTTCCATGGAATACCCCAAAA
AAAGCGGTTAACCCGTATCT

Product: DNA adenine methylase-like protein

Products: NA

Alternate protein names: M.Eco67Dam; ORF1-EC67 DAM [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSTILKWAGNKTAIMAELKKHLPEGPRLVEPFAGSCAVMIETDYPHYLVADINPDLINLYQVIKNDVEYFIKEGRYLFEA
RNDPEAYYKTRQEFNLRHGGAIERALYFLYLNRHGYRGLCRYNLDGYFNVPYGNYKKPYFPENEIRAFAEKAKRATFICA
SYDETLALLQTGDVVYCDPPYDGTFNGYHTAGFTENDQFHLASILERRSSEGHPVIVSNSDTSLTRSLYRDFTRHRITAK
RSMGVAAGDSKTAVEIIATKSACWFGVDLASGPDISVETEVRAWQ

Sequences:

>Translated_285_residues
MSTILKWAGNKTAIMAELKKHLPEGPRLVEPFAGSCAVMIETDYPHYLVADINPDLINLYQVIKNDVEYFIKEGRYLFEA
RNDPEAYYKTRQEFNLRHGGAIERALYFLYLNRHGYRGLCRYNLDGYFNVPYGNYKKPYFPENEIRAFAEKAKRATFICA
SYDETLALLQTGDVVYCDPPYDGTFNGYHTAGFTENDQFHLASILERRSSEGHPVIVSNSDTSLTRSLYRDFTRHRITAK
RSMGVAAGDSKTAVEIIATKSACWFGVDLASGPDISVETEVRAWQ
>Mature_284_residues
STILKWAGNKTAIMAELKKHLPEGPRLVEPFAGSCAVMIETDYPHYLVADINPDLINLYQVIKNDVEYFIKEGRYLFEAR
NDPEAYYKTRQEFNLRHGGAIERALYFLYLNRHGYRGLCRYNLDGYFNVPYGNYKKPYFPENEIRAFAEKAKRATFICAS
YDETLALLQTGDVVYCDPPYDGTFNGYHTAGFTENDQFHLASILERRSSEGHPVIVSNSDTSLTRSLYRDFTRHRITAKR
SMGVAAGDSKTAVEIIATKSACWFGVDLASGPDISVETEVRAWQ

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=262, Percent_Identity=46.5648854961832, Blast_Score=238, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32469; Mature: 32338

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTILKWAGNKTAIMAELKKHLPEGPRLVEPFAGSCAVMIETDYPHYLVADINPDLINLY
CCCEEECCCCCHHHHHHHHHHCCCCCCEECCCCCCEEEEEECCCCCEEEEECCCHHHHHH
QVIKNDVEYFIKEGRYLFEARNDPEAYYKTRQEFNLRHGGAIERALYFLYLNRHGYRGLC
HHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHHEEEEEEEECCCCCEEE
RYNLDGYFNVPYGNYKKPYFPENEIRAFAEKAKRATFICASYDETLALLQTGDVVYCDPP
EECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCEEEEEECCCEEEECCC
YDGTFNGYHTAGFTENDQFHLASILERRSSEGHPVIVSNSDTSLTRSLYRDFTRHRITAK
CCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
RSMGVAAGDSKTAVEIIATKSACWFGVDLASGPDISVETEVRAWQ
HHCCEECCCCCCEEEEEEECCCEEEEEECCCCCCCEEECCCEECC
>Mature Secondary Structure 
STILKWAGNKTAIMAELKKHLPEGPRLVEPFAGSCAVMIETDYPHYLVADINPDLINLY
CCEEECCCCCHHHHHHHHHHCCCCCCEECCCCCCEEEEEECCCCCEEEEECCCHHHHHH
QVIKNDVEYFIKEGRYLFEARNDPEAYYKTRQEFNLRHGGAIERALYFLYLNRHGYRGLC
HHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHHEEEEEEEECCCCCEEE
RYNLDGYFNVPYGNYKKPYFPENEIRAFAEKAKRATFICASYDETLALLQTGDVVYCDPP
EECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCEEEEEECCCEEEECCC
YDGTFNGYHTAGFTENDQFHLASILERRSSEGHPVIVSNSDTSLTRSLYRDFTRHRITAK
CCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
RSMGVAAGDSKTAVEIIATKSACWFGVDLASGPDISVETEVRAWQ
HHCCEECCCCCCEEEEEEECCCEEEEEECCCCCCCEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1701261 [H]