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The map label for this gene is nfnB

Identifier: 16763955

GI number: 16763955

Start: 637028

End: 637681

Strand: Reverse

Name: nfnB

Synonym: STM0578

Alternate gene names: 16763955

Gene position: 637681-637028 (Counterclockwise)

Preceding gene: 16763956

Following gene: 16763954

Centisome position: 13.13

GC content: 54.43

Gene sequence:

>654_bases
ATGGATATCGTTTCTGTCGCCTTACAGCGCTACTCCACTAAGGCGTTCGATCCCAGCAAAAAACTGACCGCCGAAGAAGC
GGATAAAATAAAAACACTACTACAGTACAGCCCCTCCAGCACCAATTCCCAGCCGTGGCACTTTATTGTCGCCAGTACGG
AAGAAGGCAAAGCGCGCGTAGCAAAATCCGCTGCCGGAAACTACACGTTCAACGAACGCAAAATGCTGGACGCCTCCCAT
GTGGTGGTCTTCTGCGCCAAAACCGCAATGGATGACGCATGGCTTGAGCGCGTCGTCGATCAGGAAGATGCTGATGGCCG
TTTCGCTACGCCGGAAGCTAAAGCGGCAAATGATAAAGGTCGCCGCTTTTTCGCCGATATGCACCGCGTCTCGCTGAAAG
ATGACCACCAGTGGATGGCGAAGCAGGTTTATCTGAACGTCGGCAACTTTCTGCTGGGCGTCGCCGCGATGGGTCTCGAC
GCCGTCCCCATTGAAGGTTTCGACGCCGAGGTGCTCGACGCTGAATTTGGTCTGAAAGAAAAAGGCTATACCAGTCTGGT
CGTGGTGCCGGTCGGCCATCATAGCGTCGAGGATTTCAACGCCGGGCTGCCGAAATCACGTCTGCCGCTTGAAACCACAC
TGACGGAAGTTTAA

Upstream 100 bases:

>100_bases
CTGATGCAATAACCGTTTCCGCATTTTCCTCCCTTATCTTTTTACCGATAACCCGGTACCCTGCTGATACAAAAGGGTAC
TAAACCAACCAGGAGCATTT

Downstream 100 bases:

>100_bases
TCCCCTGCCTAAGCCGGACGCCCGTCCGGCTTTTTTCACCTTTCTGCCAGGTTCCTCAATGATCAATGGCGCGAAGTGCC
AGGTTTTACATCGCTAGTGT

Product: dihydropteridine reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MDIVSVALQRYSTKAFDPSKKLTAEEADKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNYTFNERKMLDASH
VVVFCAKTAMDDAWLERVVDQEDADGRFATPEAKAANDKGRRFFADMHRVSLKDDHQWMAKQVYLNVGNFLLGVAAMGLD
AVPIEGFDAEVLDAEFGLKEKGYTSLVVVPVGHHSVEDFNAGLPKSRLPLETTLTEV

Sequences:

>Translated_217_residues
MDIVSVALQRYSTKAFDPSKKLTAEEADKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNYTFNERKMLDASH
VVVFCAKTAMDDAWLERVVDQEDADGRFATPEAKAANDKGRRFFADMHRVSLKDDHQWMAKQVYLNVGNFLLGVAAMGLD
AVPIEGFDAEVLDAEFGLKEKGYTSLVVVPVGHHSVEDFNAGLPKSRLPLETTLTEV
>Mature_217_residues
MDIVSVALQRYSTKAFDPSKKLTAEEADKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNYTFNERKMLDASH
VVVFCAKTAMDDAWLERVVDQEDADGRFATPEAKAANDKGRRFFADMHRVSLKDDHQWMAKQVYLNVGNFLLGVAAMGLD
AVPIEGFDAEVLDAEFGLKEKGYTSLVVVPVGHHSVEDFNAGLPKSRLPLETTLTEV

Specific function: Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family

Homologues:

Organism=Escherichia coli, GI1786792, Length=217, Percent_Identity=88.4792626728111, Blast_Score=400, Evalue=1e-113,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NFNB_SALTY (P15888)

Other databases:

- EMBL:   X17250
- EMBL:   AE006468
- PIR:   S08397
- RefSeq:   NP_459570.1
- PDB:   3HZN
- PDBsum:   3HZN
- ProteinModelPortal:   P15888
- SMR:   P15888
- PRIDE:   P15888
- GeneID:   1252098
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM0578
- HOGENOM:   HBG751302
- OMA:   KRYSTKA
- ProtClustDB:   PRK11053
- BioCyc:   STYP99287:STM0578-MONOMER
- InterPro:   IPR000415

Pfam domain/function: PF00881 Nitroreductase; SSF55469 Nitroreductase

EC number: 1.-.-.-; 1.5.1.34

Molecular weight: Translated: 23955; Mature: 23955

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: BINDING 41-41 BINDING 71-71

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIVSVALQRYSTKAFDPSKKLTAEEADKIKTLLQYSPSSTNSQPWHFIVASTEEGKARV
CCHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHH
AKSAAGNYTFNERKMLDASHVVVFCAKTAMDDAWLERVVDQEDADGRFATPEAKAANDKG
HHHCCCCCCCCCHHHCCCCEEEEEEEHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
RRFFADMHRVSLKDDHQWMAKQVYLNVGNFLLGVAAMGLDAVPIEGFDAEVLDAEFGLKE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCC
KGYTSLVVVPVGHHSVEDFNAGLPKSRLPLETTLTEV
CCCEEEEEEECCCCCHHHHCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MDIVSVALQRYSTKAFDPSKKLTAEEADKIKTLLQYSPSSTNSQPWHFIVASTEEGKARV
CCHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHH
AKSAAGNYTFNERKMLDASHVVVFCAKTAMDDAWLERVVDQEDADGRFATPEAKAANDKG
HHHCCCCCCCCCHHHCCCCEEEEEEEHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
RRFFADMHRVSLKDDHQWMAKQVYLNVGNFLLGVAAMGLDAVPIEGFDAEVLDAEFGLKE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCC
KGYTSLVVVPVGHHSVEDFNAGLPKSRLPLETTLTEV
CCCEEEEEEECCCCCHHHHCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2179862; 11677609