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The map label for this gene is acrA [H]

Identifier: 16763856

GI number: 16763856

Start: 532419

End: 533612

Strand: Reverse

Name: acrA [H]

Synonym: STM0476

Alternate gene names: 16763856

Gene position: 533612-532419 (Counterclockwise)

Preceding gene: 16763859

Following gene: 16763855

Centisome position: 10.99

GC content: 55.19

Gene sequence:

>1194_bases
ATGAACAAAAACAGAGGGTTAACGCCTCTGGCGGTCGTTCTGATGCTCTCAGGCAGCTTAGCGCTAACAGGATGTGACGA
CAAACAGGACCAGCAAGGCGGCCAGCAGATGCCAGAAGTTGGGGTTGTCACACTAAAAACGGAACCACTGCAAATCACAA
CTGAACTTCCGGGTCGTACCGTTGCTTACCGTATCGCCGAAGTTCGCCCGCAGGTAAGCGGCATTATCCTGAAGCGTAAT
TTCGTTGAGGGAAGTGATATCGAAGCGGGAGTCTCTCTCTATCAGATTGATCCTGCGACCTACCAGGCGACTTACGACAG
CGCTAAGGGCGATCTGGCAAAAGCGCAGGCCGCCGCGAATATCGCTGAACTGACGGTGAAGCGTTATCAAAAGCTGCTGG
GTACGCAGTACATCAGTAAGCAGGAATACGATCAGGCGCTGGCTGACGCACAACAAGCGACTGCCGCTGTTGTCGCAGCA
AAAGCCGCCGTTGAAACCGCACGTATCAACCTGGCGTATACCAAAGTCACCTCGCCGATTAGCGGTCGTATTGGTAAGTC
ATCCGTAACGGAAGGCGCACTGGTACAGAACGGTCAGGCGTCGGCGCTGGCGACAGTGCAGCAGCTGGACCCTATTTATG
TCGATGTGACCCAGTCCAGCAATGACTTCCTGCGCCTGAAGCAGGAGCTGGCAAATGGTTCGCTGAAACAGGAAAACGGC
AAAGCGAAGGTCGACCTGGTGACCAGCGACGGTATCAAATTCCCGCAGTCCGGTACGCTTGAGTTCTCCGACGTGACCGT
TGACCAAACCACCGGGTCTATTACTTTGCGCGCCATCTTCCCTAACCCGGATCACACCTTATTGCCAGGAATGTTCGTTC
GCGCACGTCTGCAGGAAGGGACAAAACCGACGGCATTACTGGTTCCACAACAGGGCGTTACCCGTACTCCACGCGGCGAT
GCCACGGTGCTGGTGGTTGGCGCTGATAACAAAGTGGAAACCCGCCAAATCGTCGCAAGCCAGGCGATCGGCGATAAGTG
GCTGGTGACTGACGGATTGAAAGCGGGCGACCGCGTAGTCGTCAGCGGGCTGCAAAAAGTACGTCCTGGCGCACAGGTTA
AAGTACAGGAAATTACCGCGGATAACAAACAGCAAGCCGCAAGCGGTGATCAACCTGCTCAGCCCAGGTCTTAA

Upstream 100 bases:

>100_bases
GGATGTATGTACCATAGCACGACGATAATATAAACGCAGCAATGGGTTTAAGGACCTTTGACCATTGACCAATTTGAAAT
CGGACACTCGAGGTTTACAT

Downstream 100 bases:

>100_bases
CTTAAACAGGAGCCGTTAAGACATGCCTAATTTCTTTATCGATCGCCCTATATTTGCGTGGGTGATCGCCATCATCATCA
TGTTGGCAGGGGGGCTCGCG

Product: acridine efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNKNRGLTPLAVVLMLSGSLALTGCDDKQDQQGGQQMPEVGVVTLKTEPLQITTELPGRTVAYRIAEVRPQVSGIILKRN
FVEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAANIAELTVKRYQKLLGTQYISKQEYDQALADAQQATAAVVAA
KAAVETARINLAYTKVTSPISGRIGKSSVTEGALVQNGQASALATVQQLDPIYVDVTQSSNDFLRLKQELANGSLKQENG
KAKVDLVTSDGIKFPQSGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLQEGTKPTALLVPQQGVTRTPRGD
ATVLVVGADNKVETRQIVASQAIGDKWLVTDGLKAGDRVVVSGLQKVRPGAQVKVQEITADNKQQAASGDQPAQPRS

Sequences:

>Translated_397_residues
MNKNRGLTPLAVVLMLSGSLALTGCDDKQDQQGGQQMPEVGVVTLKTEPLQITTELPGRTVAYRIAEVRPQVSGIILKRN
FVEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAANIAELTVKRYQKLLGTQYISKQEYDQALADAQQATAAVVAA
KAAVETARINLAYTKVTSPISGRIGKSSVTEGALVQNGQASALATVQQLDPIYVDVTQSSNDFLRLKQELANGSLKQENG
KAKVDLVTSDGIKFPQSGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLQEGTKPTALLVPQQGVTRTPRGD
ATVLVVGADNKVETRQIVASQAIGDKWLVTDGLKAGDRVVVSGLQKVRPGAQVKVQEITADNKQQAASGDQPAQPRS
>Mature_397_residues
MNKNRGLTPLAVVLMLSGSLALTGCDDKQDQQGGQQMPEVGVVTLKTEPLQITTELPGRTVAYRIAEVRPQVSGIILKRN
FVEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAANIAELTVKRYQKLLGTQYISKQEYDQALADAQQATAAVVAA
KAAVETARINLAYTKVTSPISGRIGKSSVTEGALVQNGQASALATVQQLDPIYVDVTQSSNDFLRLKQELANGSLKQENG
KAKVDLVTSDGIKFPQSGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLQEGTKPTALLVPQQGVTRTPRGD
ATVLVVGADNKVETRQIVASQAIGDKWLVTDGLKAGDRVVVSGLQKVRPGAQVKVQEITADNKQQAASGDQPAQPRS

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=397, Percent_Identity=91.6876574307305, Blast_Score=696, Evalue=0.0,
Organism=Escherichia coli, GI1789665, Length=374, Percent_Identity=68.4491978609626, Blast_Score=506, Evalue=1e-145,
Organism=Escherichia coli, GI1789929, Length=391, Percent_Identity=53.7084398976982, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI87082036, Length=321, Percent_Identity=30.5295950155763, Blast_Score=138, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42235; Mature: 42235

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNRGLTPLAVVLMLSGSLALTGCDDKQDQQGGQQMPEVGVVTLKTEPLQITTELPGRT
CCCCCCCCHHHHHHHHCCCEEEECCCCCHHHHCCCCCCCCCEEEEECCCEEEEECCCCCE
VAYRIAEVRPQVSGIILKRNFVEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAAN
EEEEHHHHCCCHHEEEEEECCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHH
IAELTVKRYQKLLGTQYISKQEYDQALADAQQATAAVVAAKAAVETARINLAYTKVTSPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCC
SGRIGKSSVTEGALVQNGQASALATVQQLDPIYVDVTQSSNDFLRLKQELANGSLKQENG
CCCCCCCCCCCCCEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCC
KAKVDLVTSDGIKFPQSGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLQEG
CEEEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
TKPTALLVPQQGVTRTPRGDATVLVVGADNKVETRQIVASQAIGDKWLVTDGLKAGDRVV
CCCEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHH
VSGLQKVRPGAQVKVQEITADNKQQAASGDQPAQPRS
HHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNKNRGLTPLAVVLMLSGSLALTGCDDKQDQQGGQQMPEVGVVTLKTEPLQITTELPGRT
CCCCCCCCHHHHHHHHCCCEEEECCCCCHHHHCCCCCCCCCEEEEECCCEEEEECCCCCE
VAYRIAEVRPQVSGIILKRNFVEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAAN
EEEEHHHHCCCHHEEEEEECCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHH
IAELTVKRYQKLLGTQYISKQEYDQALADAQQATAAVVAAKAAVETARINLAYTKVTSPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCC
SGRIGKSSVTEGALVQNGQASALATVQQLDPIYVDVTQSSNDFLRLKQELANGSLKQENG
CCCCCCCCCCCCCEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCC
KAKVDLVTSDGIKFPQSGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLQEG
CEEEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
TKPTALLVPQQGVTRTPRGDATVLVVGADNKVETRQIVASQAIGDKWLVTDGLKAGDRVV
CCCEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHH
VSGLQKVRPGAQVKVQEITADNKQQAASGDQPAQPRS
HHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]