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The map label for this gene is cof [H]

Identifier: 16763838

GI number: 16763838

Start: 514231

End: 515049

Strand: Direct

Name: cof [H]

Synonym: STM0457

Alternate gene names: 16763838

Gene position: 514231-515049 (Clockwise)

Preceding gene: 16763835

Following gene: 16763840

Centisome position: 10.59

GC content: 54.58

Gene sequence:

>819_bases
ATGGCCCGTCTGGCAGCATTTGATATGGATGGCACGTTATTAATGCCCGATCACCATTTGGGTAGAGAAACGATCGCCAC
GCTGGCGCGTCTGCGCGAACGCGATATCACCCTGACATTCGCTACGGGTCGTCATGTGCTGGAAATGCGGCATATTCTGG
GGACGCTTTCACTGGATGCTTATCTTATTACCGGTAACGGGACGCGAATTCACTCGCTGGAAGGCGACGTGCTGCATCGT
CAGGATCTCGATCCTCAGGTTGCCGATACGGTGATGCACCATGCCTGGGATACCCGAGCCAGTATGCATGTGTTTAATGA
CAACGGCTGGTTTACAGGCCAGGAGATCCCCGCGCTACTCCAGGCGCACGTCTACAGCGGCTTTCGTTATCAGGTTATCG
ATATAAAAAGCATTCCGGCCCATCAGGTGACGAAGATCTGTTTTTGCGGCGATCATGACGATCTGATCCGTTTGCGCATT
CAGCTCAATGAGGCGCTGGAAGAACGCGCGCACCTCTGTTTCTCTGCCGTCGACTGCCTGGAAGTGCTACCGCTGGGGTG
CAATAAAGGATCGGCGCTGGCGGTATTGAGCAACCATTTAGGGCTTTCACTGGCAGATTGCATGGCGTTTGGCGACGCGA
TGAACGATCGGGAAATGCTGGGCAGCGTCGGGCGGGGGCTCATTATGGGCAATGCCATGCCGCAGCTTATCGCCGCGCTG
CCACACCTTTCGGTTATCGGACATTGTGGTAATCAAGCGGTATCACACTTTTTGACGCATTGGCTGGACAATCCGCATCT
ACCTTATTCCCCCGAATGA

Upstream 100 bases:

>100_bases
AGAAACCCGGAACACTTCCTTTTATCTGTTCACTATTACTTCCGTATATCTCAGGTAATACTGAACAACAAGTTAACCGC
GTCACAGTGTGGAGAAAGAG

Downstream 100 bases:

>100_bases
GAGATCTCTTCCAGCAAGCCAGACATTCAGTCTGGCTTTTTTTTGGTTTCTAGTCGCCGGTAAGTAATTTTGCGATGGCC
GCTGACCACGGCGTCAAATC

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MARLAAFDMDGTLLMPDHHLGRETIATLARLRERDITLTFATGRHVLEMRHILGTLSLDAYLITGNGTRIHSLEGDVLHR
QDLDPQVADTVMHHAWDTRASMHVFNDNGWFTGQEIPALLQAHVYSGFRYQVIDIKSIPAHQVTKICFCGDHDDLIRLRI
QLNEALEERAHLCFSAVDCLEVLPLGCNKGSALAVLSNHLGLSLADCMAFGDAMNDREMLGSVGRGLIMGNAMPQLIAAL
PHLSVIGHCGNQAVSHFLTHWLDNPHLPYSPE

Sequences:

>Translated_272_residues
MARLAAFDMDGTLLMPDHHLGRETIATLARLRERDITLTFATGRHVLEMRHILGTLSLDAYLITGNGTRIHSLEGDVLHR
QDLDPQVADTVMHHAWDTRASMHVFNDNGWFTGQEIPALLQAHVYSGFRYQVIDIKSIPAHQVTKICFCGDHDDLIRLRI
QLNEALEERAHLCFSAVDCLEVLPLGCNKGSALAVLSNHLGLSLADCMAFGDAMNDREMLGSVGRGLIMGNAMPQLIAAL
PHLSVIGHCGNQAVSHFLTHWLDNPHLPYSPE
>Mature_271_residues
ARLAAFDMDGTLLMPDHHLGRETIATLARLRERDITLTFATGRHVLEMRHILGTLSLDAYLITGNGTRIHSLEGDVLHRQ
DLDPQVADTVMHHAWDTRASMHVFNDNGWFTGQEIPALLQAHVYSGFRYQVIDIKSIPAHQVTKICFCGDHDDLIRLRIQ
LNEALEERAHLCFSAVDCLEVLPLGCNKGSALAVLSNHLGLSLADCMAFGDAMNDREMLGSVGRGLIMGNAMPQLIAALP
HLSVIGHCGNQAVSHFLTHWLDNPHLPYSPE

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081741, Length=272, Percent_Identity=81.25, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI48994981, Length=260, Percent_Identity=37.3076923076923, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1786982, Length=278, Percent_Identity=28.0575539568345, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 30104; Mature: 29973

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLAAFDMDGTLLMPDHHLGRETIATLARLRERDITLTFATGRHVLEMRHILGTLSLDA
CCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCEEE
YLITGNGTRIHSLEGDVLHRQDLDPQVADTVMHHAWDTRASMHVFNDNGWFTGQEIPALL
EEEECCCCEEEECCCCCHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEECCHHHHHHH
QAHVYSGFRYQVIDIKSIPAHQVTKICFCGDHDDLIRLRIQLNEALEERAHLCFSAVDCL
HHHHHCCCEEEEEEECCCCHHHHEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
EVLPLGCNKGSALAVLSNHLGLSLADCMAFGDAMNDREMLGSVGRGLIMGNAMPQLIAAL
HHCCCCCCCCCEEEEEHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCEEECCHHHHHHHHC
PHLSVIGHCGNQAVSHFLTHWLDNPHLPYSPE
CHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ARLAAFDMDGTLLMPDHHLGRETIATLARLRERDITLTFATGRHVLEMRHILGTLSLDA
CCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCEEE
YLITGNGTRIHSLEGDVLHRQDLDPQVADTVMHHAWDTRASMHVFNDNGWFTGQEIPALL
EEEECCCCEEEECCCCCHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEECCHHHHHHH
QAHVYSGFRYQVIDIKSIPAHQVTKICFCGDHDDLIRLRIQLNEALEERAHLCFSAVDCL
HHHHHCCCEEEEEEECCCCHHHHEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
EVLPLGCNKGSALAVLSNHLGLSLADCMAFGDAMNDREMLGSVGRGLIMGNAMPQLIAAL
HHCCCCCCCCCEEEEEHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCEEECCHHHHHHHHC
PHLSVIGHCGNQAVSHFLTHWLDNPHLPYSPE
CHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA