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The map label for this gene is thiL

Identifier: 16763799

GI number: 16763799

Start: 472025

End: 473002

Strand: Direct

Name: thiL

Synonym: STM0419

Alternate gene names: 16763799

Gene position: 472025-473002 (Clockwise)

Preceding gene: 16763798

Following gene: 16763800

Centisome position: 9.72

GC content: 56.54

Gene sequence:

>978_bases
ATGGCATGTGGCGAGTTTTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGCTCTCGTCTTGATGTTGAAACCGGTAT
TGGCGACGATTGCGCGCTCCTGAATATTCCTGAAAAGCAGACCCTGGCGATCAGTACCGATACGCTGGTGGCGGGCAACC
ACTTCCTCCCTGATATCGATCCTGCCGATCTGGCTTATAAAGCGTTGGCGGTGAATTTAAGCGATCTGGCCGCGATGGGG
GCCGACCCGGCCTGGTTAACGCTGGCGTTAACGCTACCGGAAGTGGATGAGCCGTGGCTGGAGGCTTTTAGCGACAGCCT
GTTTGCGCTATTGAATTACTACGATATGCAGCTCATTGGCGGCGACACCACGCGTGGACCGCTGTCGATGACGCTGGGTA
TCCACGGTTATATCCCGGCAGGGCGCGCGCTCAAGCGATCTGGCGCGAAGCCGGGCGACTGGATTTATGTCACCGGTACG
CCGGGCGACAGCGCTGCCGGGCTGGCGGTTTTGCAAAACCGGCTACAGGTGTCGGAGGAGACCGATGCTCACTATTTAAT
TCAACGGCATTTACGTCCGACGCCGCGTATTTTACACGGCCAGGCGCTGCGTGACATCGCCAGCGCGGCTATCGATCTTT
CCGATGGTCTGATTTCCGATCTGGGGCATATCGTCAAGGCCAGCGGCTGTGGGGCTAGAGTGGATGTTGATGCGTTGCCA
AAATCCGATGCGATGATGCGCCATGTTGATGACGGGCAGGCGCTGCGTTGGGCGCTTTCCGGCGGCGAAGACTATGAGCT
GTGCTTTACGGTGCCGGAACTGAACCGTGGCGCGCTGGATGTCGCCATTGGTCAACTGGGTGTTCCCTTTACCTGCATAG
GCCAAATGAGCGCAGATATTGAAGGGCTGAATTTTGTACGTGACGGAATGCCTGTCACCTTCGACTGGAAAGGATATGAC
CATTTTGCCACGCCATAA

Upstream 100 bases:

>100_bases
GATTCGTCCCAACAAAAAGTGATTCACAGGCCGGAAAGATAGCGGGCGCTCGATACCCGCCGTCTCCGGCCTTTTCTTTT
TTTCTGCTGAGGCATAACGT

Downstream 100 bases:

>100_bases
AGATGTTGCGAAAAGTCGCCTGAATTTGCGCAATCCGTGGCACCTGCTCGCTACCGGCTTTGGCAGCGGGCTGAGCCCCG
TGGTGCCGGGCACGATGGGC

Product: thiamine monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MACGEFSLIARYFDRVRSSRLDVETGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMG
ADPAWLTLALTLPEVDEPWLEAFSDSLFALLNYYDMQLIGGDTTRGPLSMTLGIHGYIPAGRALKRSGAKPGDWIYVTGT
PGDSAAGLAVLQNRLQVSEETDAHYLIQRHLRPTPRILHGQALRDIASAAIDLSDGLISDLGHIVKASGCGARVDVDALP
KSDAMMRHVDDGQALRWALSGGEDYELCFTVPELNRGALDVAIGQLGVPFTCIGQMSADIEGLNFVRDGMPVTFDWKGYD
HFATP

Sequences:

>Translated_325_residues
MACGEFSLIARYFDRVRSSRLDVETGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMG
ADPAWLTLALTLPEVDEPWLEAFSDSLFALLNYYDMQLIGGDTTRGPLSMTLGIHGYIPAGRALKRSGAKPGDWIYVTGT
PGDSAAGLAVLQNRLQVSEETDAHYLIQRHLRPTPRILHGQALRDIASAAIDLSDGLISDLGHIVKASGCGARVDVDALP
KSDAMMRHVDDGQALRWALSGGEDYELCFTVPELNRGALDVAIGQLGVPFTCIGQMSADIEGLNFVRDGMPVTFDWKGYD
HFATP
>Mature_324_residues
ACGEFSLIARYFDRVRSSRLDVETGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGA
DPAWLTLALTLPEVDEPWLEAFSDSLFALLNYYDMQLIGGDTTRGPLSMTLGIHGYIPAGRALKRSGAKPGDWIYVTGTP
GDSAAGLAVLQNRLQVSEETDAHYLIQRHLRPTPRILHGQALRDIASAAIDLSDGLISDLGHIVKASGCGARVDVDALPK
SDAMMRHVDDGQALRWALSGGEDYELCFTVPELNRGALDVAIGQLGVPFTCIGQMSADIEGLNFVRDGMPVTFDWKGYDH
FATP

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family

Homologues:

Organism=Escherichia coli, GI1786619, Length=325, Percent_Identity=88, Blast_Score=586, Evalue=1e-169,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIL_SALTY (P55881)

Other databases:

- EMBL:   U74758
- EMBL:   AE006468
- PIR:   T47117
- RefSeq:   NP_459414.1
- ProteinModelPortal:   P55881
- SMR:   P55881
- PRIDE:   P55881
- GeneID:   1251938
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM0419
- NMPDR:   fig|99287.1.peg.404
- HOGENOM:   HBG577902
- OMA:   MGAEPAW
- ProtClustDB:   PRK05731
- BioCyc:   STYP99287:STM0419-MONOMER
- BRENDA:   2.7.4.16
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283
- PIRSF:   PIRSF005303
- TIGRFAMs:   TIGR01379

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: =2.7.4.16

Molecular weight: Translated: 34986; Mature: 34855

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACGEFSLIARYFDRVRSSRLDVETGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDID
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCEEEECCCCCCCCC
PADLAYKALAVNLSDLAAMGADPAWLTLALTLPEVDEPWLEAFSDSLFALLNYYDMQLIG
HHHHHHHHHHCCHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEC
GDTTRGPLSMTLGIHGYIPAGRALKRSGAKPGDWIYVTGTPGDSAAGLAVLQNRLQVSEE
CCCCCCCEEEEEEEEECCCCCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCC
TDAHYLIQRHLRPTPRILHGQALRDIASAAIDLSDGLISDLGHIVKASGCGARVDVDALP
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEHHCCC
KSDAMMRHVDDGQALRWALSGGEDYELCFTVPELNRGALDVAIGQLGVPFTCIGQMSADI
CCCHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEEHHCCCCCEEEECCCCCCC
EGLNFVRDGMPVTFDWKGYDHFATP
CCCHHHHCCCCEEEECCCCCCCCCC
>Mature Secondary Structure 
ACGEFSLIARYFDRVRSSRLDVETGIGDDCALLNIPEKQTLAISTDTLVAGNHFLPDID
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCEEEECCCCCCCCC
PADLAYKALAVNLSDLAAMGADPAWLTLALTLPEVDEPWLEAFSDSLFALLNYYDMQLIG
HHHHHHHHHHCCHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEC
GDTTRGPLSMTLGIHGYIPAGRALKRSGAKPGDWIYVTGTPGDSAAGLAVLQNRLQVSEE
CCCCCCCEEEEEEEEECCCCCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCC
TDAHYLIQRHLRPTPRILHGQALRDIASAAIDLSDGLISDLGHIVKASGCGARVDVDALP
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEHHCCC
KSDAMMRHVDDGQALRWALSGGEDYELCFTVPELNRGALDVAIGQLGVPFTCIGQMSADI
CCCHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEEHHCCCCCEEEECCCCCCC
EGLNFVRDGMPVTFDWKGYDHFATP
CCCHHHHCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609