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The map label for this gene is leuD2 [H]

Identifier: 16763710

GI number: 16763710

Start: 374196

End: 374822

Strand: Direct

Name: leuD2 [H]

Synonym: STM0330

Alternate gene names: 16763710

Gene position: 374196-374822 (Clockwise)

Preceding gene: 16763709

Following gene: 16763711

Centisome position: 7.7

GC content: 52.31

Gene sequence:

>627_bases
ATGGATACGTTTAAGCAAATCAGCGGGCGAATTGCGCCGATGCTGGAACCGAATATCGACACTGATGTGATTATGCCAAA
ACAGTTCCTGAAAGGGATCGATCGCCAGGGGCTGGATAAAGGGGTGTTTTTCGATCGTCGCTTTATGGCTGGCGGCCAAC
CAAATCCTGATTTTATTCTGAACATGCCGGGCTGGCAGAGCGCGACATTCCTGCTGGTTGGCCCCAATTTTGGCTGTGGA
TCGAGTCGTGAACACGCCGTATGGGGATTAAAACAACTGGGAGTACGGGGGCTGATAGGTAGCACATTTGCAGGCATTTT
CGATGATAACTGCCAACGCAACGGGATCCTCACGGTCTCATTAGATGAACCTGCTTTGGCGCGCCTGGCGCAGCTTGCCG
CGAGTGCTGATACCAATTCTATCACTGTTTCACTCGATCGCTGCGAAATTACGACGGCAGAGGAAACGATCTCCTTTGTG
ATCAGTGAACTCAAACGGGCGATGCTGGCCGCAGGCGAAGATGCGATAGCCTGGACATTGCAATACTTGCCGGAGATTGA
AAATTTTGAGGTTGCGCATTATTCACGGCGGCCGTGGCTAAAACGCCCTGCATCCCCGCGCGGTTAA

Upstream 100 bases:

>100_bases
GGAGCAAGAACGCATTTGATGAGCCCGGCGATGGTTGCGGCGGCGGCGGTAGCGGGGCATCTGGTCGATGTTCGGTCATT
ACTTCAGGCAGGGGAATAAG

Downstream 100 bases:

>100_bases
GGAAGAAGACTGCAATGAAACTGGTTCAATATCTTGTGAACGGTGGCAAACGCTACGGCATTATGCAGGAAACCGGAATA
ATTGATCTCTCGCAGCGGCT

Product: 3-isopropylmalate isomerase

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MDTFKQISGRIAPMLEPNIDTDVIMPKQFLKGIDRQGLDKGVFFDRRFMAGGQPNPDFILNMPGWQSATFLLVGPNFGCG
SSREHAVWGLKQLGVRGLIGSTFAGIFDDNCQRNGILTVSLDEPALARLAQLAASADTNSITVSLDRCEITTAEETISFV
ISELKRAMLAAGEDAIAWTLQYLPEIENFEVAHYSRRPWLKRPASPRG

Sequences:

>Translated_208_residues
MDTFKQISGRIAPMLEPNIDTDVIMPKQFLKGIDRQGLDKGVFFDRRFMAGGQPNPDFILNMPGWQSATFLLVGPNFGCG
SSREHAVWGLKQLGVRGLIGSTFAGIFDDNCQRNGILTVSLDEPALARLAQLAASADTNSITVSLDRCEITTAEETISFV
ISELKRAMLAAGEDAIAWTLQYLPEIENFEVAHYSRRPWLKRPASPRG
>Mature_208_residues
MDTFKQISGRIAPMLEPNIDTDVIMPKQFLKGIDRQGLDKGVFFDRRFMAGGQPNPDFILNMPGWQSATFLLVGPNFGCG
SSREHAVWGLKQLGVRGLIGSTFAGIFDDNCQRNGILTVSLDEPALARLAQLAASADTNSITVSLDRCEITTAEETISFV
ISELKRAMLAAGEDAIAWTLQYLPEIENFEVAHYSRRPWLKRPASPRG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=191, Percent_Identity=39.2670157068063, Blast_Score=141, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321429, Length=209, Percent_Identity=36.3636363636364, Blast_Score=128, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22933; Mature: 22933

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTFKQISGRIAPMLEPNIDTDVIMPKQFLKGIDRQGLDKGVFFDRRFMAGGQPNPDFIL
CCCHHHHCCCCCCCCCCCCCCCEECHHHHHHHCCCCCCCCCCEECHHHHCCCCCCCCEEE
NMPGWQSATFLLVGPNFGCGSSREHAVWGLKQLGVRGLIGSTFAGIFDDNCQRNGILTVS
ECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCEEEEE
LDEPALARLAQLAASADTNSITVSLDRCEITTAEETISFVISELKRAMLAAGEDAIAWTL
ECCHHHHHHHHHHHCCCCCEEEEEEECEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
QYLPEIENFEVAHYSRRPWLKRPASPRG
HHCCCCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MDTFKQISGRIAPMLEPNIDTDVIMPKQFLKGIDRQGLDKGVFFDRRFMAGGQPNPDFIL
CCCHHHHCCCCCCCCCCCCCCCEECHHHHHHHCCCCCCCCCCEECHHHHCCCCCCCCEEE
NMPGWQSATFLLVGPNFGCGSSREHAVWGLKQLGVRGLIGSTFAGIFDDNCQRNGILTVS
ECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCEEEEE
LDEPALARLAQLAASADTNSITVSLDRCEITTAEETISFVISELKRAMLAAGEDAIAWTL
ECCHHHHHHHHHHHCCCCCEEEEEEECEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
QYLPEIENFEVAHYSRRPWLKRPASPRG
HHCCCCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA