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The map label for this gene is leuC2 [H]

Identifier: 16763709

GI number: 16763709

Start: 372773

End: 374194

Strand: Direct

Name: leuC2 [H]

Synonym: STM0329

Alternate gene names: 16763709

Gene position: 372773-374194 (Clockwise)

Preceding gene: 39546292

Following gene: 16763710

Centisome position: 7.67

GC content: 51.41

Gene sequence:

>1422_bases
ATGTCTGCTAAAACGCTGTATGAAAAATTAGTTGAGTCACATACCATTCGTGAACTTGATAACGAAGGACATGTTTTACT
TTATATTGATCGTTCAATACTGAATGAATATACCAGCCCGCAGGCATTTAGCGGGTTAAGAGAACGCGGCCGCACCGTTC
GGCACCCCGACACGTTTTTATTGAATATTGACCATGTTAATCCTACGCGGTCGCAACGGGATGACTTAATGACCGATCCG
GGAGGACAGCTACAGGTTGATTACTTTCGGGAAAATGCCGCTGACTTTGGCATCACATTATTTGATGTGCTGGATCCGCG
TCAGGGGATTGAACACGTGGTTGCTCATGAACAAGGGCTAGTGATGCCTGGCATGGTGATCGCCGCCGGGGATAGTCACA
CGACAACCTACGGTGCATTTGGCGCGCTGGGCTTTGGTATCGGGACGTCGGAAATTGAACATCTGCTGGCCACGCAGACG
CTGGTTTACCGCAAGCTCAAAACCATGCGCGTAAGTGTTCAGGGAGAGCTACCTTTCGCCTGTTCCGCAAAAGATATTGT
TCTTGAATTGCTTGAACGTATTGGCGCTGATGGCGCGACGGGATATGCCATTGAATTTGTGGGTGAGGCCATCAGCGCGT
TGAGCGTTGAAGGACGAATGACGCTCTGCAACATGGCGGTAGAAGCGGGGGCGCGTGGTGCCATTATTGCGCCGGATAAA
AAGGTTTTTGACTATATCTACGGTAAACCGCAAATGCCTGTAGGTGAACTCTGGCAGCAGGCATTGCTGGAATGGTCACA
GTTGAGCAGCGATGCTGATGCGGTATTTGATAAGACGGTGGCGATCAACTGCCATGATCTTGAGCCTAAAGTCACCTGGG
GAATCAGTCCGGATCAAACAGGTTCCATCACCGGACGTGTTCCTTTCCCGGAACAGGAAACCAATCCGCTTAAGCGCCTG
GCGCTTGAGAAGGCCCTTCATTACATGGGATTAACGGCTGGCATGTTGCTGAAAGATATTCGTATCTCTCACGCTTTTAT
TGGTTCATGCACAAATGGTCGCATTGAGGATTTGCGTGCCGTTGCGAAGGTGCTTGAAGGGCGCAAAATCGCCAGCCATG
TTCGCGGGATCATTGTGCCTGGTTCAACAATGGTAAGACGCCAGGCGGAAGAAGAAGGACTGGCGAAAATCTTTATTGCT
GCCGGTTTTGAATGGCGGCAGTCGGGTTGCTCAATGTGCCTTGCGATGAATGAAGATGTATTGTCGCCCGGCGATCGCTG
TGCATCAGGTACGAACCGTAACTTCCCTGGACGACAGGGCGCTGGAGCAAGAACGCATTTGATGAGCCCGGCGATGGTTG
CGGCGGCGGCGGTAGCGGGGCATCTGGTCGATGTTCGGTCATTACTTCAGGCAGGGGAATAA

Upstream 100 bases:

>100_bases
CATAATGGAATCATCATTTTTTCATTAATGTGTCACTATAGCTAAAAGCTTAAATTGTGAATATTTGGCTACCCGGATAT
TCGTTGCTCCAGGAATATAT

Downstream 100 bases:

>100_bases
GATGGATACGTTTAAGCAAATCAGCGGGCGAATTGCGCCGATGCTGGAACCGAATATCGACACTGATGTGATTATGCCAA
AACAGTTCCTGAAAGGGATC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSAKTLYEKLVESHTIRELDNEGHVLLYIDRSILNEYTSPQAFSGLRERGRTVRHPDTFLLNIDHVNPTRSQRDDLMTDP
GGQLQVDYFRENAADFGITLFDVLDPRQGIEHVVAHEQGLVMPGMVIAAGDSHTTTYGAFGALGFGIGTSEIEHLLATQT
LVYRKLKTMRVSVQGELPFACSAKDIVLELLERIGADGATGYAIEFVGEAISALSVEGRMTLCNMAVEAGARGAIIAPDK
KVFDYIYGKPQMPVGELWQQALLEWSQLSSDADAVFDKTVAINCHDLEPKVTWGISPDQTGSITGRVPFPEQETNPLKRL
ALEKALHYMGLTAGMLLKDIRISHAFIGSCTNGRIEDLRAVAKVLEGRKIASHVRGIIVPGSTMVRRQAEEEGLAKIFIA
AGFEWRQSGCSMCLAMNEDVLSPGDRCASGTNRNFPGRQGAGARTHLMSPAMVAAAAVAGHLVDVRSLLQAGE

Sequences:

>Translated_473_residues
MSAKTLYEKLVESHTIRELDNEGHVLLYIDRSILNEYTSPQAFSGLRERGRTVRHPDTFLLNIDHVNPTRSQRDDLMTDP
GGQLQVDYFRENAADFGITLFDVLDPRQGIEHVVAHEQGLVMPGMVIAAGDSHTTTYGAFGALGFGIGTSEIEHLLATQT
LVYRKLKTMRVSVQGELPFACSAKDIVLELLERIGADGATGYAIEFVGEAISALSVEGRMTLCNMAVEAGARGAIIAPDK
KVFDYIYGKPQMPVGELWQQALLEWSQLSSDADAVFDKTVAINCHDLEPKVTWGISPDQTGSITGRVPFPEQETNPLKRL
ALEKALHYMGLTAGMLLKDIRISHAFIGSCTNGRIEDLRAVAKVLEGRKIASHVRGIIVPGSTMVRRQAEEEGLAKIFIA
AGFEWRQSGCSMCLAMNEDVLSPGDRCASGTNRNFPGRQGAGARTHLMSPAMVAAAAVAGHLVDVRSLLQAGE
>Mature_472_residues
SAKTLYEKLVESHTIRELDNEGHVLLYIDRSILNEYTSPQAFSGLRERGRTVRHPDTFLLNIDHVNPTRSQRDDLMTDPG
GQLQVDYFRENAADFGITLFDVLDPRQGIEHVVAHEQGLVMPGMVIAAGDSHTTTYGAFGALGFGIGTSEIEHLLATQTL
VYRKLKTMRVSVQGELPFACSAKDIVLELLERIGADGATGYAIEFVGEAISALSVEGRMTLCNMAVEAGARGAIIAPDKK
VFDYIYGKPQMPVGELWQQALLEWSQLSSDADAVFDKTVAINCHDLEPKVTWGISPDQTGSITGRVPFPEQETNPLKRLA
LEKALHYMGLTAGMLLKDIRISHAFIGSCTNGRIEDLRAVAKVLEGRKIASHVRGIIVPGSTMVRRQAEEEGLAKIFIAA
GFEWRQSGCSMCLAMNEDVLSPGDRCASGTNRNFPGRQGAGARTHLMSPAMVAAAAVAGHLVDVRSLLQAGE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=474, Percent_Identity=25.3164556962025, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI41352693, Length=405, Percent_Identity=25.9259259259259, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=52.1459227467811, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI87081781, Length=357, Percent_Identity=24.6498599439776, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787531, Length=399, Percent_Identity=24.812030075188, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25149337, Length=365, Percent_Identity=27.3972602739726, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=365, Percent_Identity=27.3972602739726, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=304, Percent_Identity=27.3026315789474, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=433, Percent_Identity=24.2494226327945, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=50.3184713375796, Blast_Score=482, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6320440, Length=462, Percent_Identity=24.6753246753247, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=365, Percent_Identity=27.3972602739726, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=384, Percent_Identity=27.34375, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI281365315, Length=384, Percent_Identity=26.3020833333333, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI17864292, Length=384, Percent_Identity=26.3020833333333, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161076999, Length=384, Percent_Identity=26.3020833333333, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28571643, Length=473, Percent_Identity=25.1585623678647, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24645686, Length=380, Percent_Identity=26.5789473684211, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17137564, Length=453, Percent_Identity=24.7240618101545, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51446; Mature: 51315

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKTLYEKLVESHTIRELDNEGHVLLYIDRSILNEYTSPQAFSGLRERGRTVRHPDTFL
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCHHHHHHHHCCCCCCCCCEEE
LNIDHVNPTRSQRDDLMTDPGGQLQVDYFRENAADFGITLFDVLDPRQGIEHVVAHEQGL
EEECCCCCCCHHHHCCCCCCCCCEEEHHHHCCCCCCCEEHHHHCCHHHHHHHHHHCCCCC
VMPGMVIAAGDSHTTTYGAFGALGFGIGTSEIEHLLATQTLVYRKLKTMRVSVQGELPFA
CCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
CSAKDIVLELLERIGADGATGYAIEFVGEAISALSVEGRMTLCNMAVEAGARGAIIAPDK
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEECCCH
KVFDYIYGKPQMPVGELWQQALLEWSQLSSDADAVFDKTVAINCHDLEPKVTWGISPDQT
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCEEEEEEECCCCCEEECCCCCCC
GSITGRVPFPEQETNPLKRLALEKALHYMGLTAGMLLKDIRISHAFIGSCTNGRIEDLRA
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VAKVLEGRKIASHVRGIIVPGSTMVRRQAEEEGLAKIFIAAGFEWRQSGCSMCLAMNEDV
HHHHHCCHHHHHHHCEEEECCHHHHHHHHHHCCCEEEEEECCCHHHHCCCCEEEECCCCC
LSPGDRCASGTNRNFPGRQGAGARTHLMSPAMVAAAAVAGHLVDVRSLLQAGE
CCCCHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAKTLYEKLVESHTIRELDNEGHVLLYIDRSILNEYTSPQAFSGLRERGRTVRHPDTFL
CHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCHHHHHHHHCCCCCCCCCEEE
LNIDHVNPTRSQRDDLMTDPGGQLQVDYFRENAADFGITLFDVLDPRQGIEHVVAHEQGL
EEECCCCCCCHHHHCCCCCCCCCEEEHHHHCCCCCCCEEHHHHCCHHHHHHHHHHCCCCC
VMPGMVIAAGDSHTTTYGAFGALGFGIGTSEIEHLLATQTLVYRKLKTMRVSVQGELPFA
CCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
CSAKDIVLELLERIGADGATGYAIEFVGEAISALSVEGRMTLCNMAVEAGARGAIIAPDK
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEECCCH
KVFDYIYGKPQMPVGELWQQALLEWSQLSSDADAVFDKTVAINCHDLEPKVTWGISPDQT
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCEEEEEEECCCCCEEECCCCCCC
GSITGRVPFPEQETNPLKRLALEKALHYMGLTAGMLLKDIRISHAFIGSCTNGRIEDLRA
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VAKVLEGRKIASHVRGIIVPGSTMVRRQAEEEGLAKIFIAAGFEWRQSGCSMCLAMNEDV
HHHHHCCHHHHHHHCEEEECCHHHHHHHHHHCCCEEEEEECCCHHHHCCCCEEEECCCCC
LSPGDRCASGTNRNFPGRQGAGARTHLMSPAMVAAAAVAGHLVDVRSLLQAGE
CCCCHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA