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The map label for this gene is safB [H]

Identifier: 16763683

GI number: 16763683

Start: 342829

End: 343542

Strand: Direct

Name: safB [H]

Synonym: STM0300

Alternate gene names: 16763683

Gene position: 342829-343542 (Clockwise)

Preceding gene: 16763682

Following gene: 16763684

Centisome position: 7.06

GC content: 48.88

Gene sequence:

>714_bases
ATGGCGGTAGCTTTGTTCGCCACTAATTCTATGGTTTCAGTATATGCCGTCAACCAGCAATTAAATTCAGCCACTAAATT
ATTCAGCGTGAAGCTGGGGGCTACACGAGTGATTTATCACGCTGGTACGGCTGGAGCGACGCTCTCGGTGAGCAACCCGC
AGAATTACCCTATTTTGGTTCAGTCTTCAGTCAAAGCAGCAGACAAAAGTTCGCCTGCTCCCTTTTTGGTGATGCCGCCT
CTATTTCGTTTAGAAGCAAACCAGCAGAGTCAACTGCGTATTGTCCGTACTGGTGGTGACATGCCAACGGATCGTGAGAC
TTTACAGTGGGTCTGTATAAAGGCGGTACCACCCGAAAATGAACCGTCGGATACACAGGCTAAGGGCGCGACCCTTGACC
TCAATTTGTCCATCAACGCCTGTGATAAGCTGATTTTCCGCCCGGATGCCGTGAAGGGGACGCCGGAAGATGTTGCAGGA
AATTTAAGATGGGTGGAGACGGGCAACAAACTTAAGGTGGAGAACCCCACCCCGTTTTACATGAATTTAGCCTCTGTCAC
AGTAGGGGGAAAGCCCATTACAGGGCTTGAGTATGTCCCCCCCTTTGCTGACAAAACACTAAATATGCCAGGTAGTGCCC
ATGGTGATATCGAGTGGAGAGTTATTACAGACTTTGGTGGTGAAAGTCATCCGTTCCACTACGTTCTTAAATAA

Upstream 100 bases:

>100_bases
CAACTATTAGTGTATTGTGCCTTGTTTAAGGCGCAATACACATCAAATCATCTATTTTTCTTTTACAATTTTTGATATGA
AAATAGTTAATTTTGCTGTA

Downstream 100 bases:

>100_bases
ATCCAGGGGCTTAGCGGCAGAAAATGAAGTTCAAACAACCTGCCTTGCTACTGTTCATCGCGGGAGTGGTTCATTGCGCA
AATGCGCACACTTACACATT

Product: fimbrial assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MAVALFATNSMVSVYAVNQQLNSATKLFSVKLGATRVIYHAGTAGATLSVSNPQNYPILVQSSVKAADKSSPAPFLVMPP
LFRLEANQQSQLRIVRTGGDMPTDRETLQWVCIKAVPPENEPSDTQAKGATLDLNLSINACDKLIFRPDAVKGTPEDVAG
NLRWVETGNKLKVENPTPFYMNLASVTVGGKPITGLEYVPPFADKTLNMPGSAHGDIEWRVITDFGGESHPFHYVLK

Sequences:

>Translated_237_residues
MAVALFATNSMVSVYAVNQQLNSATKLFSVKLGATRVIYHAGTAGATLSVSNPQNYPILVQSSVKAADKSSPAPFLVMPP
LFRLEANQQSQLRIVRTGGDMPTDRETLQWVCIKAVPPENEPSDTQAKGATLDLNLSINACDKLIFRPDAVKGTPEDVAG
NLRWVETGNKLKVENPTPFYMNLASVTVGGKPITGLEYVPPFADKTLNMPGSAHGDIEWRVITDFGGESHPFHYVLK
>Mature_236_residues
AVALFATNSMVSVYAVNQQLNSATKLFSVKLGATRVIYHAGTAGATLSVSNPQNYPILVQSSVKAADKSSPAPFLVMPPL
FRLEANQQSQLRIVRTGGDMPTDRETLQWVCIKAVPPENEPSDTQAKGATLDLNLSINACDKLIFRPDAVKGTPEDVAGN
LRWVETGNKLKVENPTPFYMNLASVTVGGKPITGLEYVPPFADKTLNMPGSAHGDIEWRVITDFGGESHPFHYVLK

Specific function: Involved in the biogenesis of the AAF/I fimbriae [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1789532, Length=202, Percent_Identity=35.6435643564356, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1790771, Length=200, Percent_Identity=35.5, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787171, Length=202, Percent_Identity=30.1980198019802, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1786333, Length=241, Percent_Identity=28.2157676348548, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786743, Length=198, Percent_Identity=29.7979797979798, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87081806, Length=140, Percent_Identity=32.8571428571429, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786936, Length=217, Percent_Identity=27.1889400921659, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 25656; Mature: 25524

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVALFATNSMVSVYAVNQQLNSATKLFSVKLGATRVIYHAGTAGATLSVSNPQNYPILV
CEEEEEECCCEEEEEEECCHHCCCEEEEEEEECCEEEEEECCCCCCEEEECCCCCCCEEE
QSSVKAADKSSPAPFLVMPPLFRLEANQQSQLRIVRTGGDMPTDRETLQWVCIKAVPPEN
ECCCCCCCCCCCCCEEEECCCEEECCCCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCC
EPSDTQAKGATLDLNLSINACDKLIFRPDAVKGTPEDVAGNLRWVETGNKLKVENPTPFY
CCCCCCCCCCEEEEEEEHHCCCEEEECCCCCCCCHHHHCCCEEEEECCCEEEECCCCCEE
MNLASVTVGGKPITGLEYVPPFADKTLNMPGSAHGDIEWRVITDFGGESHPFHYVLK
EEEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEEECCCCCCCEEEEEC
>Mature Secondary Structure 
AVALFATNSMVSVYAVNQQLNSATKLFSVKLGATRVIYHAGTAGATLSVSNPQNYPILV
EEEEEECCCEEEEEEECCHHCCCEEEEEEEECCEEEEEECCCCCCEEEECCCCCCCEEE
QSSVKAADKSSPAPFLVMPPLFRLEANQQSQLRIVRTGGDMPTDRETLQWVCIKAVPPEN
ECCCCCCCCCCCCCEEEECCCEEECCCCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCC
EPSDTQAKGATLDLNLSINACDKLIFRPDAVKGTPEDVAGNLRWVETGNKLKVENPTPFY
CCCCCCCCCCEEEEEEEHHCCCEEEECCCCCCCCHHHHCCCEEEEECCCEEEECCCCCEE
MNLASVTVGGKPITGLEYVPPFADKTLNMPGSAHGDIEWRVITDFGGESHPFHYVLK
EEEEEEEECCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEEECCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7914189 [H]