| Definition | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome. |
|---|---|
| Accession | NC_003197 |
| Length | 4,857,432 |
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The map label for this gene is dkgB [H]
Identifier: 16763638
GI number: 16763638
Start: 295069
End: 295872
Strand: Direct
Name: dkgB [H]
Synonym: STM0255
Alternate gene names: 16763638
Gene position: 295069-295872 (Clockwise)
Preceding gene: 16763637
Following gene: 16763640
Centisome position: 6.07
GC content: 51.12
Gene sequence:
>804_bases ATGACTATCCCTGCATTCGGTTTAGGTACTTTTCGCCTGAAAGACGACGTGGTTATCGCTTCTGTTAAAACGGCGCTTGA ACTGGGCTACCGTGCTGTCGACACTGCGCAAATCTATGATAATGAAGCGGCTGTTGGACAGGCGATTGCTGAGAGCGGCG TACCGCGTAATGAGCTGTACATCACCACCAAGATCTGGATTGAAAACCTTAGCAAAGACAAGCTAATCCCAAGCCTGAAA GAGAGTCTGAAAAAACTGCGTACCGATTATGTCGATCTGACGCTGATCCACTGGCCATCTCCGGGTGATGCCGTGTCTGT CGAAGAATTTATGCAGGCATTGCTTGAAGCTAAAAAACAAGGGCTGACTCGCGAAATTGGCATTTCAAACTTCACCATTC CGTTGATGGAAAAGGCTATTGCCGCGGTGGGCGCAGACAATATCGCCACCAACCAGATTGAGCTATCTCCGTATCTGCAG AATCGTAAGGTGGTTGACTGGGCGAAAGCGCACGGTATCCACATCACCTCTTATATGACGCTGGCATACGGTAAGGCGCT GAAAGATGAAGTGATTGCGCGCATTGCGGCTAAACATAATGCGACCCCGGCGCAGGTTATTCTGGCATGGGCAATGGGTG AAGGTTACTCCGTGATCCCTTCATCCACCAGGCGTGAAAATCTGGCAAGCAGCCTGCTGGCGCAGGATCTGCATCTGGAT GCCGAAGATAAAAATGCGATCGCGGCACTGGACTGCAACGATCGTCTGGTTAGCCCGGAAGGACTGGCTCCTGCCTGGGA TTAA
Upstream 100 bases:
>100_bases ATTCGCAAAAATCCTCTGCATTTCTCGCTCTTTTTCTGCAAAGCGCTGAAGGTCATAATCCCCTCAGTTAACGAACATAG TGATTAAAAAGAGGAATTCT
Downstream 100 bases:
>100_bases TTACCCGATCCTTTGTACCTTTACCCCTCTTTCACAGCTCCTGCGGGAGCTGTTCTTATATATTCGCTCAAAAAGTCGAT AAATGCCCGAATACGTGTGC
Product: 2,5-diketo-D-gluconate reductase B
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 267; Mature: 266
Protein sequence:
>267_residues MTIPAFGLGTFRLKDDVVIASVKTALELGYRAVDTAQIYDNEAAVGQAIAESGVPRNELYITTKIWIENLSKDKLIPSLK ESLKKLRTDYVDLTLIHWPSPGDAVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGADNIATNQIELSPYLQ NRKVVDWAKAHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTRRENLASSLLAQDLHLD AEDKNAIAALDCNDRLVSPEGLAPAWD
Sequences:
>Translated_267_residues MTIPAFGLGTFRLKDDVVIASVKTALELGYRAVDTAQIYDNEAAVGQAIAESGVPRNELYITTKIWIENLSKDKLIPSLK ESLKKLRTDYVDLTLIHWPSPGDAVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGADNIATNQIELSPYLQ NRKVVDWAKAHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTRRENLASSLLAQDLHLD AEDKNAIAALDCNDRLVSPEGLAPAWD >Mature_266_residues TIPAFGLGTFRLKDDVVIASVKTALELGYRAVDTAQIYDNEAAVGQAIAESGVPRNELYITTKIWIENLSKDKLIPSLKE SLKKLRTDYVDLTLIHWPSPGDAVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGADNIATNQIELSPYLQN RKVVDWAKAHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTRRENLASSLLAQDLHLDA EDKNAIAALDCNDRLVSPEGLAPAWD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI310109922, Length=275, Percent_Identity=31.2727272727273, Blast_Score=140, Evalue=1e-33, Organism=Homo sapiens, GI310109920, Length=292, Percent_Identity=29.7945205479452, Blast_Score=138, Evalue=6e-33, Organism=Homo sapiens, GI45446745, Length=292, Percent_Identity=29.7945205479452, Blast_Score=137, Evalue=7e-33, Organism=Homo sapiens, GI4503285, Length=292, Percent_Identity=29.7945205479452, Blast_Score=137, Evalue=7e-33, Organism=Homo sapiens, GI5453543, Length=292, Percent_Identity=29.4520547945205, Blast_Score=136, Evalue=2e-32, Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=29.4117647058824, Blast_Score=134, Evalue=6e-32, Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=29.4117647058824, Blast_Score=134, Evalue=6e-32, Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=28.8659793814433, Blast_Score=134, Evalue=8e-32, Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=30.3754266211604, Blast_Score=131, Evalue=8e-31, Organism=Homo sapiens, GI223468663, Length=285, Percent_Identity=30.5263157894737, Blast_Score=130, Evalue=1e-30, Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=29.6296296296296, Blast_Score=130, Evalue=1e-30, Organism=Homo sapiens, GI93277124, Length=299, Percent_Identity=30.1003344481605, Blast_Score=128, Evalue=4e-30, Organism=Homo sapiens, GI4502049, Length=288, Percent_Identity=32.9861111111111, Blast_Score=125, Evalue=3e-29, Organism=Homo sapiens, GI300116273, Length=268, Percent_Identity=29.8507462686567, Blast_Score=120, Evalue=2e-27, Organism=Homo sapiens, GI291291012, Length=270, Percent_Identity=29.6296296296296, Blast_Score=119, Evalue=4e-27, Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=29.8561151079137, Blast_Score=111, Evalue=5e-25, Organism=Homo sapiens, GI310109926, Length=114, Percent_Identity=37.719298245614, Blast_Score=79, Evalue=4e-15, Organism=Homo sapiens, GI207028673, Length=114, Percent_Identity=37.719298245614, Blast_Score=79, Evalue=4e-15, Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=27.741935483871, Blast_Score=72, Evalue=4e-13, Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=92.5093632958801, Blast_Score=506, Evalue=1e-145, Organism=Escherichia coli, GI87082198, Length=257, Percent_Identity=38.5214007782101, Blast_Score=181, Evalue=4e-47, Organism=Escherichia coli, GI1788081, Length=240, Percent_Identity=30, Blast_Score=104, Evalue=7e-24, Organism=Escherichia coli, GI48994888, Length=238, Percent_Identity=28.9915966386555, Blast_Score=75, Evalue=7e-15, Organism=Escherichia coli, GI1787674, Length=257, Percent_Identity=26.8482490272374, Blast_Score=73, Evalue=2e-14, Organism=Caenorhabditis elegans, GI17550248, Length=271, Percent_Identity=35.4243542435424, Blast_Score=150, Evalue=5e-37, Organism=Caenorhabditis elegans, GI17537075, Length=291, Percent_Identity=35.7388316151203, Blast_Score=140, Evalue=5e-34, Organism=Caenorhabditis elegans, GI17537077, Length=289, Percent_Identity=35.6401384083045, Blast_Score=140, Evalue=5e-34, Organism=Caenorhabditis elegans, GI71998625, Length=266, Percent_Identity=31.5789473684211, Blast_Score=136, Evalue=1e-32, Organism=Caenorhabditis elegans, GI17552492, Length=267, Percent_Identity=30.3370786516854, Blast_Score=132, Evalue=2e-31, Organism=Caenorhabditis elegans, GI17561298, Length=277, Percent_Identity=29.9638989169675, Blast_Score=130, Evalue=9e-31, Organism=Caenorhabditis elegans, GI17561300, Length=276, Percent_Identity=30.4347826086957, Blast_Score=129, Evalue=2e-30, Organism=Caenorhabditis elegans, GI17566692, Length=279, Percent_Identity=30.8243727598566, Blast_Score=119, Evalue=1e-27, Organism=Caenorhabditis elegans, GI17538386, Length=281, Percent_Identity=30.2491103202847, Blast_Score=116, Evalue=1e-26, Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=26.530612244898, Blast_Score=113, Evalue=8e-26, Organism=Caenorhabditis elegans, GI17564128, Length=292, Percent_Identity=26.027397260274, Blast_Score=109, Evalue=1e-24, Organism=Caenorhabditis elegans, GI17562292, Length=238, Percent_Identity=30.2521008403361, Blast_Score=108, Evalue=2e-24, Organism=Saccharomyces cerevisiae, GI6324694, Length=289, Percent_Identity=34.9480968858132, Blast_Score=159, Evalue=5e-40, Organism=Saccharomyces cerevisiae, GI6320576, Length=264, Percent_Identity=35.6060606060606, Blast_Score=149, Evalue=3e-37, Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=28.7581699346405, Blast_Score=126, Evalue=4e-30, Organism=Saccharomyces cerevisiae, GI6322556, Length=250, Percent_Identity=26.4, Blast_Score=114, Evalue=2e-26, Organism=Saccharomyces cerevisiae, GI6319625, Length=267, Percent_Identity=30.3370786516854, Blast_Score=110, Evalue=3e-25, Organism=Saccharomyces cerevisiae, GI6320079, Length=243, Percent_Identity=29.6296296296296, Blast_Score=95, Evalue=1e-20, Organism=Drosophila melanogaster, GI24644950, Length=297, Percent_Identity=32.6599326599327, Blast_Score=139, Evalue=3e-33, Organism=Drosophila melanogaster, GI24662789, Length=294, Percent_Identity=31.2925170068027, Blast_Score=136, Evalue=1e-32, Organism=Drosophila melanogaster, GI221378297, Length=318, Percent_Identity=31.1320754716981, Blast_Score=131, Evalue=5e-31, Organism=Drosophila melanogaster, GI24657054, Length=284, Percent_Identity=30.9859154929577, Blast_Score=126, Evalue=2e-29, Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=34.2560553633218, Blast_Score=124, Evalue=8e-29, Organism=Drosophila melanogaster, GI20129731, Length=287, Percent_Identity=30.3135888501742, Blast_Score=120, Evalue=7e-28, Organism=Drosophila melanogaster, GI45553081, Length=271, Percent_Identity=30.2583025830258, Blast_Score=120, Evalue=8e-28, Organism=Drosophila melanogaster, GI24663317, Length=288, Percent_Identity=29.1666666666667, Blast_Score=120, Evalue=1e-27, Organism=Drosophila melanogaster, GI24662781, Length=289, Percent_Identity=33.5640138408304, Blast_Score=119, Evalue=1e-27, Organism=Drosophila melanogaster, GI21356425, Length=289, Percent_Identity=29.757785467128, Blast_Score=116, Evalue=2e-26, Organism=Drosophila melanogaster, GI281366140, Length=259, Percent_Identity=27.4131274131274, Blast_Score=100, Evalue=2e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 29154; Mature: 29022
Theoretical pI: Translated: 5.17; Mature: 5.17
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 1.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTIPAFGLGTFRLKDDVVIASVKTALELGYRAVDTAQIYDNEAAVGQAIAESGVPRNELY CCCCCCCCEEEEEECCEEEHHHHHHHHHCCHHCCHHHHHCCHHHHHHHHHHCCCCCCCEE ITTKIWIENLSKDKLIPSLKESLKKLRTDYVDLTLIHWPSPGDAVSVEEFMQALLEAKKQ EEEEHEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC GLTREIGISNFTIPLMEKAIAAVGADNIATNQIELSPYLQNRKVVDWAKAHGIHITSYMT CCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCCEEEHHHHCCEEEHHHHH LAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTRRENLASSLLAQDLHLD HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHCCC AEDKNAIAALDCNDRLVSPEGLAPAWD CCCCCCEEEECCCCCEECCCCCCCCCC >Mature Secondary Structure TIPAFGLGTFRLKDDVVIASVKTALELGYRAVDTAQIYDNEAAVGQAIAESGVPRNELY CCCCCCCEEEEEECCEEEHHHHHHHHHCCHHCCHHHHHCCHHHHHHHHHHCCCCCCCEE ITTKIWIENLSKDKLIPSLKESLKKLRTDYVDLTLIHWPSPGDAVSVEEFMQALLEAKKQ EEEEHEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC GLTREIGISNFTIPLMEKAIAAVGADNIATNQIELSPYLQNRKVVDWAKAHGIHITSYMT CCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCCEEEHHHHCCEEEHHHHH LAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTRRENLASSLLAQDLHLD HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHCCC AEDKNAIAALDCNDRLVSPEGLAPAWD CCCCCCEEEECCCCCEECCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796; 11108008 [H]