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The map label for this gene is trmE [H]

Identifier: 167626215

GI number: 167626215

Start: 5222712

End: 5224073

Strand: Reverse

Name: trmE [H]

Synonym: Shal_4310

Alternate gene names: 167626215

Gene position: 5224073-5222712 (Counterclockwise)

Preceding gene: 167626216

Following gene: 167626214

Centisome position: 99.95

GC content: 46.92

Gene sequence:

>1362_bases
ATGACTACAGATACTATCGTGGCACAAGCGACCGCTCCAGGACGTGGTGGCGTAGGTATTATTCGCGTATCTGGCGACTT
AGCGAGTAATGTTGCTATGGCTGTTTTAGGGCATATTCCTAAGACCCGCTACGCCGATTACTGTGACTTTAAAGAAGATA
GCGGCGAAGTTATCGACCAAGGTATCGCGCTATTCTTTAAAGGCCCAAACTCCTTTACCGGTGAAGATGTACTCGAGCTT
CAGGGGCACGGCGGTCAAATCGTTCTCGATATGCTGATTAAACGTGTTATGGAAGTTGATGGCCTGCGTATTGCGAAACC
AGGAGAATTCAGCGAACAAGCTTTCATGAATGATAAGCTTGACCTAACTCAGGCCGAAGCCATTGCCGACTTAATTGATG
CCACCAGCGAACAAGCCGCCAAAAGCGCTCTAAACTCACTTCAGGGTGAATTCTCTACTCAGGTACATGATTTAGTAGAA
AAGGTCACCAACCTGCGTCTATACGTCGAGGCAGCAATTGATTTCCCTGATGAAGAGGTCGACTTTCTCTCTGATGGTAA
AATTGCCGCGTCTTTAAATGGTATTATCGGTAAACTTGATGGTGTTCAAGCCAGCGCAAAACAAGGTTCAATCATACGTG
AAGGTATGAAAGTGGTAATTGCGGGTCGACCTAATGCTGGCAAATCGAGCTTATTGAATGCCCTTGCAGGTAAAGAATCG
GCTATTGTGACCGAAATAGCCGGTACCACTCGTGATGTACTGCGTGAACATATCCATTTAGACGGTATGCCACTGCACAT
TATCGATACCGCAGGCCTGCGCGATACCGCCGATACCGTCGAAAAAATTGGTATTGAACGCGCGTGGGATGAGATCAGAA
CTGCCGATCGAGTGCTGTTTATGGTCGATGGCACCACCACTCCCGCGGTTGATCCTCATGAAATCTGGCCAGACTTTATC
GATCGCCTACCTAACAACTTAGGTGTGACGGTGGTGCGTAATAAAGCCGATCTTACTGGTGAAGATCTTGCTATCACAAC
TGAAGCAGGTCATAGCGTTTATCGTATCTCAGCCAAGACAGGTTTAGGGGTCGACGATCTCAAGCAGCACCTAAAATCAT
TGATGGGTTACCAAAGTAACCTTGAGGGTGGCTTTATCGCTCGTAGACGTCATTTAGAAGCACTCGACTTGGCAAGCAGC
CACTTACTTCTAGGTAAAGAACAATTAGAAGTGTATCAAGCGGGCGAGTTACTCGCCGAAGAGCTACGCATGTGCCAGAT
GGCGTTATCTGAAATAACCGGTAAATTTACTTCTGACGACCTGCTAGGTAAGATCTTTAGTTCATTCTGTATCGGCAAGT
AA

Upstream 100 bases:

>100_bases
AACAAAAAGCGGTCTAATACTGAGTATTGACCGCTTTTTTGCTTTCTATATAGAAGCCGTATTTTTATAAACACCTGAAA
TTAGATTAGGAAACACAGTA

Downstream 100 bases:

>100_bases
AACCGCTCTAATTAAGAATAATCCCACAAAATATAAAGCCACAATAGACTTAACTACTGTGGCTTTTTGTTTATCTAAAT
TATGATCATGTTGCAGATAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTTDTIVAQATAPGRGGVGIIRVSGDLASNVAMAVLGHIPKTRYADYCDFKEDSGEVIDQGIALFFKGPNSFTGEDVLEL
QGHGGQIVLDMLIKRVMEVDGLRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALNSLQGEFSTQVHDLVE
KVTNLRLYVEAAIDFPDEEVDFLSDGKIAASLNGIIGKLDGVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTADTVEKIGIERAWDEIRTADRVLFMVDGTTTPAVDPHEIWPDFI
DRLPNNLGVTVVRNKADLTGEDLAITTEAGHSVYRISAKTGLGVDDLKQHLKSLMGYQSNLEGGFIARRRHLEALDLASS
HLLLGKEQLEVYQAGELLAEELRMCQMALSEITGKFTSDDLLGKIFSSFCIGK

Sequences:

>Translated_453_residues
MTTDTIVAQATAPGRGGVGIIRVSGDLASNVAMAVLGHIPKTRYADYCDFKEDSGEVIDQGIALFFKGPNSFTGEDVLEL
QGHGGQIVLDMLIKRVMEVDGLRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALNSLQGEFSTQVHDLVE
KVTNLRLYVEAAIDFPDEEVDFLSDGKIAASLNGIIGKLDGVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTADTVEKIGIERAWDEIRTADRVLFMVDGTTTPAVDPHEIWPDFI
DRLPNNLGVTVVRNKADLTGEDLAITTEAGHSVYRISAKTGLGVDDLKQHLKSLMGYQSNLEGGFIARRRHLEALDLASS
HLLLGKEQLEVYQAGELLAEELRMCQMALSEITGKFTSDDLLGKIFSSFCIGK
>Mature_452_residues
TTDTIVAQATAPGRGGVGIIRVSGDLASNVAMAVLGHIPKTRYADYCDFKEDSGEVIDQGIALFFKGPNSFTGEDVLELQ
GHGGQIVLDMLIKRVMEVDGLRIAKPGEFSEQAFMNDKLDLTQAEAIADLIDATSEQAAKSALNSLQGEFSTQVHDLVEK
VTNLRLYVEAAIDFPDEEVDFLSDGKIAASLNGIIGKLDGVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKESA
IVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTADTVEKIGIERAWDEIRTADRVLFMVDGTTTPAVDPHEIWPDFID
RLPNNLGVTVVRNKADLTGEDLAITTEAGHSVYRISAKTGLGVDDLKQHLKSLMGYQSNLEGGFIARRRHLEALDLASSH
LLLGKEQLEVYQAGELLAEELRMCQMALSEITGKFTSDDLLGKIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=473, Percent_Identity=33.8266384778013, Blast_Score=216, Evalue=4e-56,
Organism=Homo sapiens, GI193082993, Length=463, Percent_Identity=34.341252699784, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI193082988, Length=505, Percent_Identity=31.6831683168317, Blast_Score=202, Evalue=4e-52,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=72.9490022172949, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=92, Percent_Identity=38.0434782608696, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=469, Percent_Identity=29.2110874200426, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=30.1810865191147, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=31.7124735729387, Blast_Score=211, Evalue=6e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 48885; Mature: 48754

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDTIVAQATAPGRGGVGIIRVSGDLASNVAMAVLGHIPKTRYADYCDFKEDSGEVIDQ
CCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHC
GIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIKRVMEVDGLRIAKPGEFSEQAFMNDKL
CEEEEEECCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHCCCEECCCCCCCHHHHCCCCC
DLTQAEAIADLIDATSEQAAKSALNSLQGEFSTQVHDLVEKVTNLRLYVEAAIDFPDEEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCC
DFLSDGKIAASLNGIIGKLDGVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
CCCCCCCCEEHHHHHHHCCCCCCCCCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCC
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTADTVEKIGIERAWDEIRTADRVLF
HHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
MVDGTTTPAVDPHEIWPDFIDRLPNNLGVTVVRNKADLTGEDLAITTEAGHSVYRISAKT
EECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEEECCC
GLGVDDLKQHLKSLMGYQSNLEGGFIARRRHLEALDLASSHLLLGKEQLEVYQAGELLAE
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
ELRMCQMALSEITGKFTSDDLLGKIFSSFCIGK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTDTIVAQATAPGRGGVGIIRVSGDLASNVAMAVLGHIPKTRYADYCDFKEDSGEVIDQ
CCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHC
GIALFFKGPNSFTGEDVLELQGHGGQIVLDMLIKRVMEVDGLRIAKPGEFSEQAFMNDKL
CEEEEEECCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHCCCEECCCCCCCHHHHCCCCC
DLTQAEAIADLIDATSEQAAKSALNSLQGEFSTQVHDLVEKVTNLRLYVEAAIDFPDEEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCC
DFLSDGKIAASLNGIIGKLDGVQASAKQGSIIREGMKVVIAGRPNAGKSSLLNALAGKES
CCCCCCCCEEHHHHHHHCCCCCCCCCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCCC
AIVTEIAGTTRDVLREHIHLDGMPLHIIDTAGLRDTADTVEKIGIERAWDEIRTADRVLF
HHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
MVDGTTTPAVDPHEIWPDFIDRLPNNLGVTVVRNKADLTGEDLAITTEAGHSVYRISAKT
EECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEEECCC
GLGVDDLKQHLKSLMGYQSNLEGGFIARRRHLEALDLASSHLLLGKEQLEVYQAGELLAE
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
ELRMCQMALSEITGKFTSDDLLGKIFSSFCIGK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA