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The map label for this gene is ppsA [H]

Identifier: 167623636

GI number: 167623636

Start: 2046344

End: 2048713

Strand: Direct

Name: ppsA [H]

Synonym: Shal_1705

Alternate gene names: 167623636

Gene position: 2046344-2048713 (Clockwise)

Preceding gene: 167623631

Following gene: 167623637

Centisome position: 39.15

GC content: 45.57

Gene sequence:

>2370_bases
GTGCAGCAATACGTACTCTGGTATCAAGAATTAGGCATGGGTGACGTCAACAAGGTAGGCGGTAAGAACGCTTCTCTTGG
AGAGATGATTAGCAATCTATCAAACGCAGGCGTTCAAGTGCCTGGTGGATTTGCGACAACATCTTTTGCGTTCAATGAAT
TTCTTGAACAAAGTGGACTAAACCAGAAGATATACGAAATTCTAGAAACTCTAGATGTAGATGACGTGAATGCACTCGCT
ACAGCAGGAGCACAGATCAGACAGTGGGTTATCGATACCCCGTTCCACCCAGAATTTGAGCAAGCGGTACGTGAGTCTTA
CGACAAGCTTAGCTCTGAAACAAATGACGCATCATTTGCAGTTCGCTCTTCAGCAACAGCAGAAGATATGCCAGATGCAT
CATTTGCTGGTCAACAAGAAACTTTCCTAAACGTTAAGGGTTATGAAGCCGTGTTAGAAGCAACTAAGCATGTATTTGCT
TCTTTGTTTAATGACCGTGCTATTTCATACCGTGTTCACCAAGGTTACGACCATAAAGGCGTCGCACTATCAGCGGGTGT
TCAGCGCATGGTGCGTTCAGATAAAGCGGCTTCTGGTGTTATGTTCACCATGGACACTGAGTCTGGCAACAATGACGTGG
TATTTATTACTTCTTCATTCGGCCTAGGTGAGATGGTTGTTCAAGGTGCGGTTAACCCTGACGAATTTTATGTTCATAAG
CCAACTTTGGTTGCAGGACATAAAGCGGTTGTACGCCGTAACATCGGTAGCAAGCTTATCCAGATGGTGTATTCAGACGA
TGAATCTCATGGTAAGCAAGTTAAGATTGAAGATATCGCTGCTGACAAGCGTCGTGAATTCTCAATTAACGATGCTGAAG
TACAAGAACTTGCTAAGCAAGCAATGACTATCGAGAAGCACTACGGTCGTCCAATGGACATCGAATGGGCTAAAGACGGT
AACGACGGTAAGTTGTATATCGTACAGGCTCGTCCTGAAACTGTTCGTTCTCGTGAAGATGTACAGCTAATTGAACGTTA
CCACCTTAAGACTCAAGGTGACGTCGTTTGTGAAGGCCGTGCAATTGGTCACAAGATTGGTAAAGGTGTCGCTAAGGTAC
TTAAGTCAATCGAAGACATGGATCAAATTCAACCAGGTGATGTGTTAGTTACCGACATGACGGACCCAGATTGGGAGCCA
ATCATGAAGCGTGCAAGCGCGATTGTAACTAACCGTGGTGGCCGTACTTGTCACGCCGCGATTATCGCACGTGAGCTTGG
CGTTCCTGCTGTTGTTGGTTGTGGCGACGTAACTGAGCGTATCAAAAATGGCCAAGAGATTACCGTTTCTTGTGCTGAAG
GTGACACAGGCCTTATCTACAATGGTCTACAAGACTTTGAAGTGATTTCATCACGTGTAGATTCAATGCCTGAATTGCCA
ATGAAGATTACGATGAACGTGGGTAACCCAGACCGCGCATTTGACTTTGCACGTCTACCTAACGAAGGTGTTGGTCTTGC
ACGTCTTGAGTTCATCATCAATCGCATGATTGGTATTCACCCTAAAGCATTGCTTGAGTTCAACGACCAAACTGCAGAGC
TTCAACAAGAGATCCACGAGATGATCGCGGGTTATGAATCTCCAGTTGAATTTTACGTGGCACGTTTAGTTGAAGGTATT
GCAACAATTGCTTCAGCTTTCCACCCGAAGAAAGTCATTGTTCGTATGTCTGACTTTAAGTCAAATGAATACGCAAACTT
GGTAGGTGGTGACCGTTACGAGCCAGAGGAAGAAAACCCAATGTTGGGCTTCCGCGGTGCGAGCCGCTATATCTCTGAAT
CGTTCCGTGATTGTTTTGCACTAGAGTGTGAAGCGATTAAGCGTGTTCGTAATGAGATGGGCCTTAAAAACGTTGAAATC
ATGATCCCATTCGTTCGCACATTGAGCGAAGCAGCGCAGGTGATTGAGCTTCTTAAAGAGCAAGGTCTTGAGCGTGGTAA
AGATGGTTTACGCGTTATCATGATGTGTGAGTTGCCTTCAAACGCACTGCTTGCTGAACAGTTCCTTGAGCACTTCGATG
GTTTCTCTATCGGCTCAAACGACTTAACGCAGCTAACTCTTGGTCTTGACCGTGACTCAGGCATCATCAGCCATCTATTT
GATGAGCGTAATGACGCAGTTAAAGCCTTGCTTTCCATGGCAATTAAAGCGGCAAAAGCTAAGGGCGCATACGTTGGTAT
TTGTGGGCAAGGCCCATCAGACCATCCAGACTTTGCGGCTTGGTTGGTTGAGCAAGGCATCGATACTGTATCGCTAAACC
CAGATACCGTTATCGATACTTGGTTATACCTAGCTGAAGCTCACGGTTAA

Upstream 100 bases:

>100_bases
CAAATAAAATTATACATTTACGCTTCTTGCCATGGAGTGTAAGATGCTGTTGCTCTCGTGTGAAGAGCCACATTAAAATA
TACTTGCGGAGATAAGAACT

Downstream 100 bases:

>100_bases
TATTATCCATGTAAGCTTATAGTCTAAATGAAGTGTTAAATCTGTGCGATTTAACATTTTGTTAAAAACATGAAAAAAGC
CGCTATTTAGCGGCTTTTTT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 789; Mature: 789

Protein sequence:

>789_residues
MQQYVLWYQELGMGDVNKVGGKNASLGEMISNLSNAGVQVPGGFATTSFAFNEFLEQSGLNQKIYEILETLDVDDVNALA
TAGAQIRQWVIDTPFHPEFEQAVRESYDKLSSETNDASFAVRSSATAEDMPDASFAGQQETFLNVKGYEAVLEATKHVFA
SLFNDRAISYRVHQGYDHKGVALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PTLVAGHKAVVRRNIGSKLIQMVYSDDESHGKQVKIEDIAADKRREFSINDAEVQELAKQAMTIEKHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKTQGDVVCEGRAIGHKIGKGVAKVLKSIEDMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTERIKNGQEITVSCAEGDTGLIYNGLQDFEVISSRVDSMPELP
MKITMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNDQTAELQQEIHEMIAGYESPVEFYVARLVEGI
ATIASAFHPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNEMGLKNVEI
MIPFVRTLSEAAQVIELLKEQGLERGKDGLRVIMMCELPSNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERNDAVKALLSMAIKAAKAKGAYVGICGQGPSDHPDFAAWLVEQGIDTVSLNPDTVIDTWLYLAEAHG

Sequences:

>Translated_789_residues
MQQYVLWYQELGMGDVNKVGGKNASLGEMISNLSNAGVQVPGGFATTSFAFNEFLEQSGLNQKIYEILETLDVDDVNALA
TAGAQIRQWVIDTPFHPEFEQAVRESYDKLSSETNDASFAVRSSATAEDMPDASFAGQQETFLNVKGYEAVLEATKHVFA
SLFNDRAISYRVHQGYDHKGVALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PTLVAGHKAVVRRNIGSKLIQMVYSDDESHGKQVKIEDIAADKRREFSINDAEVQELAKQAMTIEKHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKTQGDVVCEGRAIGHKIGKGVAKVLKSIEDMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTERIKNGQEITVSCAEGDTGLIYNGLQDFEVISSRVDSMPELP
MKITMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNDQTAELQQEIHEMIAGYESPVEFYVARLVEGI
ATIASAFHPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNEMGLKNVEI
MIPFVRTLSEAAQVIELLKEQGLERGKDGLRVIMMCELPSNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERNDAVKALLSMAIKAAKAKGAYVGICGQGPSDHPDFAAWLVEQGIDTVSLNPDTVIDTWLYLAEAHG
>Mature_789_residues
MQQYVLWYQELGMGDVNKVGGKNASLGEMISNLSNAGVQVPGGFATTSFAFNEFLEQSGLNQKIYEILETLDVDDVNALA
TAGAQIRQWVIDTPFHPEFEQAVRESYDKLSSETNDASFAVRSSATAEDMPDASFAGQQETFLNVKGYEAVLEATKHVFA
SLFNDRAISYRVHQGYDHKGVALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PTLVAGHKAVVRRNIGSKLIQMVYSDDESHGKQVKIEDIAADKRREFSINDAEVQELAKQAMTIEKHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKTQGDVVCEGRAIGHKIGKGVAKVLKSIEDMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTERIKNGQEITVSCAEGDTGLIYNGLQDFEVISSRVDSMPELP
MKITMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNDQTAELQQEIHEMIAGYESPVEFYVARLVEGI
ATIASAFHPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNEMGLKNVEI
MIPFVRTLSEAAQVIELLKEQGLERGKDGLRVIMMCELPSNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERNDAVKALLSMAIKAAKAKGAYVGICGQGPSDHPDFAAWLVEQGIDTVSLNPDTVIDTWLYLAEAHG

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=782, Percent_Identity=75.4475703324808, Blast_Score=1268, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=448, Percent_Identity=27.4553571428571, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1788726, Length=190, Percent_Identity=32.1052631578947, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI48994992, Length=397, Percent_Identity=25.6926952141058, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789193, Length=390, Percent_Identity=25.3846153846154, Blast_Score=90, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17564524, Length=314, Percent_Identity=26.1146496815287, Blast_Score=90, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87080; Mature: 87080

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYVLWYQELGMGDVNKVGGKNASLGEMISNLSNAGVQVPGGFATTSFAFNEFLEQSGL
CCCCEEEHHHCCCCCCHHCCCCCCCHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHCCC
NQKIYEILETLDVDDVNALATAGAQIRQWVIDTPFHPEFEQAVRESYDKLSSETNDASFA
CHHHHHHHHHCCCCCHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
VRSSATAEDMPDASFAGQQETFLNVKGYEAVLEATKHVFASLFNDRAISYRVHQGYDHKG
EECCCCCCCCCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
VALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
EEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEEC
PTLVAGHKAVVRRNIGSKLIQMVYSDDESHGKQVKIEDIAADKRREFSINDAEVQELAKQ
CCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHH
AMTIEKHYGRPMDIEWAKDGNDGKLYIVQARPETVRSREDVQLIERYHLKTQGDVVCEGR
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHCCCCCCEEECCH
AIGHKIGKGVAKVLKSIEDMDQIQPGDVLVTDMTDPDWEPIMKRASAIVTNRGGRTCHAA
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
IIARELGVPAVVGCGDVTERIKNGQEITVSCAEGDTGLIYNGLQDFEVISSRVDSMPELP
HHHHHCCCCEEECCCHHHHHHCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHCCCCCCC
MKITMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNDQTAELQQEIHE
EEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHEECCCHHHHHHHHHHH
MIAGYESPVEFYVARLVEGIATIASAFHPKKVIVRMSDFKSNEYANLVGGDRYEPEEENP
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCHHCCCCCCCCCCCCCC
MLGFRGASRYISESFRDCFALECEAIKRVRNEMGLKNVEIMIPFVRTLSEAAQVIELLKE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHH
QGLERGKDGLRVIMMCELPSNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
CCHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHH
DERNDAVKALLSMAIKAAKAKGAYVGICGQGPSDHPDFAAWLVEQGIDTVSLNPDTVIDT
HCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEECCCCHHHHH
WLYLAEAHG
HHHHHHCCC
>Mature Secondary Structure
MQQYVLWYQELGMGDVNKVGGKNASLGEMISNLSNAGVQVPGGFATTSFAFNEFLEQSGL
CCCCEEEHHHCCCCCCHHCCCCCCCHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHCCC
NQKIYEILETLDVDDVNALATAGAQIRQWVIDTPFHPEFEQAVRESYDKLSSETNDASFA
CHHHHHHHHHCCCCCHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
VRSSATAEDMPDASFAGQQETFLNVKGYEAVLEATKHVFASLFNDRAISYRVHQGYDHKG
EECCCCCCCCCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
VALSAGVQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
EEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEEC
PTLVAGHKAVVRRNIGSKLIQMVYSDDESHGKQVKIEDIAADKRREFSINDAEVQELAKQ
CCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHH
AMTIEKHYGRPMDIEWAKDGNDGKLYIVQARPETVRSREDVQLIERYHLKTQGDVVCEGR
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHCCCCCCEEECCH
AIGHKIGKGVAKVLKSIEDMDQIQPGDVLVTDMTDPDWEPIMKRASAIVTNRGGRTCHAA
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
IIARELGVPAVVGCGDVTERIKNGQEITVSCAEGDTGLIYNGLQDFEVISSRVDSMPELP
HHHHHCCCCEEECCCHHHHHHCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHCCCCCCC
MKITMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNDQTAELQQEIHE
EEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHEECCCHHHHHHHHHHH
MIAGYESPVEFYVARLVEGIATIASAFHPKKVIVRMSDFKSNEYANLVGGDRYEPEEENP
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCHHCCCCCCCCCCCCCC
MLGFRGASRYISESFRDCFALECEAIKRVRNEMGLKNVEIMIPFVRTLSEAAQVIELLKE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHH
QGLERGKDGLRVIMMCELPSNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
CCHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHH
DERNDAVKALLSMAIKAAKAKGAYVGICGQGPSDHPDFAAWLVEQGIDTVSLNPDTVIDT
HCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEECCCCHHHHH
WLYLAEAHG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]