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The map label for this gene is puuB [H]

Identifier: 167623631

GI number: 167623631

Start: 2041164

End: 2042453

Strand: Direct

Name: puuB [H]

Synonym: Shal_1700

Alternate gene names: 167623631

Gene position: 2041164-2042453 (Clockwise)

Preceding gene: 167623629

Following gene: 167623636

Centisome position: 39.05

GC content: 45.27

Gene sequence:

>1290_bases
ATGTCCGAACCACGCTGTGACTCCTATTACAATGCAACTATTAATAAAGAAACCGATTATCCCGAGTTAGAACAAGAGAT
CAGAGTGGATGTGGTGATCATTGGTGGTGGTTTTACCGGTGTCGCCACAGCGCTAGAGCTGTCAGAGAAGGGGTACAAGG
TTGCGGTTTTAGAAGCGAATAAAATTGCCTGGGGAGCCACTGGGCGTAATGGTGGGCAAGTTACTGGTAGCTTGTCAGGC
GATGAAGCCATGACAAAGCAACTTAGGAATTGTCTAGGCACTGATGCTGAGCAATATGTTTGGAATATGCGCTGGCGCGG
GCATGAGATCATTAAAGAACGCGTTAATAAATATAATATCGACTGTGATTTAAAATTTGGTCACATTCATACCGCATACA
AAGCGAGCCATTTAAAAGAGCTAGAAAAGACTTATTCTGAGGGCTGCAAACGAGGCATGGAAGCTGAGTTGTCAATGTTG
AATAAAGAAGAACTCTCACAGCACCTAAATACACCCCTTTATCACGGTGGACTGCTTAATAAGCGTAATATGCACCTACA
CTCAGTAAACTTATGCATAGGTGAAGCTAGGGCGGCAGAGAGTCTCGGGGCTCTAATTTTTGAGCATTCGGCAGTTGTCG
ACATTAACGATGGTGCGTTGGTTACGGTGACAACCAATAAAGGCAAAGTCGTCGCCAATAGCGCAGTACTAGCAGGTAAC
GCGTACCATAAATTAGCCCGTAAAAAACTCCGTGGAATGTTGTTCCCTGCATCTTTAGGTAACTGTGCTACTGCGCAGTT
GCCAGCAGAATTGGCGCGGAGCCTGATCCCTAAAGATGTCGCCGTCTATGATACTCGCTTTGTACTCGACTATTACCGGT
TAACAGCCGATAACCGTTTAATGTTTGGCGGCGGAACTAACTATTCTGGTCGAGATTCAAAAAATGTTGCGCTCGAGCTG
AGACCTGCTATCGAGCGTACCTTTCCGCAACTTAAAGGTGTAGACATTGAGTTTGAATGGACTGGTATGGCGGGCATTGT
GCTTAATCGGATCCCTCAGTTAGGTAAGGTTTCGAAGAATGTTTTTTATTGCCAAGGTTATTCAGGTCATGGGGTGGCGA
CATCTCATATTATGGGAGAAGTCATGGCGAGCGCCGTAGTTGGAGAGCTACAAGAGTTTGACTTGTTTGCACAGATGCCC
CATTTTCGTATTCCACTTAATGAGTGGTTTGGAAATCAAGCTTTAGCGCTGGGCATGATGTATTACAAGGTGATGGAAAA
CTTTCGTTAA

Upstream 100 bases:

>100_bases
CTGGTCTTGATCTAATTAATTGGATTGATATACTGAACAAATCTTTAAATTTGTAAAAAATATAAAACAAGAAGTAACTG
ATTACTCATAAGGAATGGAT

Downstream 100 bases:

>100_bases
TGCTTGCTGTTGCACTTTGTGCGCTTCGAAATTAGCAAGGCAGAGAATAATAAAAATCCGCTTCGATTACTCGTTAGCGG
AATTTTTATTAGTCGCGATA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSEPRCDSYYNATINKETDYPELEQEIRVDVVIIGGGFTGVATALELSEKGYKVAVLEANKIAWGATGRNGGQVTGSLSG
DEAMTKQLRNCLGTDAEQYVWNMRWRGHEIIKERVNKYNIDCDLKFGHIHTAYKASHLKELEKTYSEGCKRGMEAELSML
NKEELSQHLNTPLYHGGLLNKRNMHLHSVNLCIGEARAAESLGALIFEHSAVVDINDGALVTVTTNKGKVVANSAVLAGN
AYHKLARKKLRGMLFPASLGNCATAQLPAELARSLIPKDVAVYDTRFVLDYYRLTADNRLMFGGGTNYSGRDSKNVALEL
RPAIERTFPQLKGVDIEFEWTGMAGIVLNRIPQLGKVSKNVFYCQGYSGHGVATSHIMGEVMASAVVGELQEFDLFAQMP
HFRIPLNEWFGNQALALGMMYYKVMENFR

Sequences:

>Translated_429_residues
MSEPRCDSYYNATINKETDYPELEQEIRVDVVIIGGGFTGVATALELSEKGYKVAVLEANKIAWGATGRNGGQVTGSLSG
DEAMTKQLRNCLGTDAEQYVWNMRWRGHEIIKERVNKYNIDCDLKFGHIHTAYKASHLKELEKTYSEGCKRGMEAELSML
NKEELSQHLNTPLYHGGLLNKRNMHLHSVNLCIGEARAAESLGALIFEHSAVVDINDGALVTVTTNKGKVVANSAVLAGN
AYHKLARKKLRGMLFPASLGNCATAQLPAELARSLIPKDVAVYDTRFVLDYYRLTADNRLMFGGGTNYSGRDSKNVALEL
RPAIERTFPQLKGVDIEFEWTGMAGIVLNRIPQLGKVSKNVFYCQGYSGHGVATSHIMGEVMASAVVGELQEFDLFAQMP
HFRIPLNEWFGNQALALGMMYYKVMENFR
>Mature_428_residues
SEPRCDSYYNATINKETDYPELEQEIRVDVVIIGGGFTGVATALELSEKGYKVAVLEANKIAWGATGRNGGQVTGSLSGD
EAMTKQLRNCLGTDAEQYVWNMRWRGHEIIKERVNKYNIDCDLKFGHIHTAYKASHLKELEKTYSEGCKRGMEAELSMLN
KEELSQHLNTPLYHGGLLNKRNMHLHSVNLCIGEARAAESLGALIFEHSAVVDINDGALVTVTTNKGKVVANSAVLAGNA
YHKLARKKLRGMLFPASLGNCATAQLPAELARSLIPKDVAVYDTRFVLDYYRLTADNRLMFGGGTNYSGRDSKNVALELR
PAIERTFPQLKGVDIEFEWTGMAGIVLNRIPQLGKVSKNVFYCQGYSGHGVATSHIMGEVMASAVVGELQEFDLFAQMPH
FRIPLNEWFGNQALALGMMYYKVMENFR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=427, Percent_Identity=39.1100702576112, Blast_Score=318, Evalue=5e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47602; Mature: 47471

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPRCDSYYNATINKETDYPELEQEIRVDVVIIGGGFTGVATALELSEKGYKVAVLEAN
CCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEC
KIAWGATGRNGGQVTGSLSGDEAMTKQLRNCLGTDAEQYVWNMRWRGHEIIKERVNKYNI
EEEECCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHEECCCCCHHHHHHHHHHCCC
DCDLKFGHIHTAYKASHLKELEKTYSEGCKRGMEAELSMLNKEELSQHLNTPLYHGGLLN
EEEEEECCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCCCCCCCCCC
KRNMHLHSVNLCIGEARAAESLGALIFEHSAVVDINDGALVTVTTNKGKVVANSAVLAGN
CCCCEEEEEEEEECCHHHHHHHHHHHEECCEEEEECCCEEEEEECCCCEEEECCEEEECH
AYHKLARKKLRGMLFPASLGNCATAQLPAELARSLIPKDVAVYDTRFVLDYYRLTADNRL
HHHHHHHHHHHCEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHEEEHHEECCCCEE
MFGGGTNYSGRDSKNVALELRPAIERTFPQLKGVDIEFEWTGMAGIVLNRIPQLGKVSKN
EECCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCCCC
VFYCQGYSGHGVATSHIMGEVMASAVVGELQEFDLFAQMPHFRIPLNEWFGNQALALGMM
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCCHHHHHHHH
YYKVMENFR
HHHHHHHCC
>Mature Secondary Structure 
SEPRCDSYYNATINKETDYPELEQEIRVDVVIIGGGFTGVATALELSEKGYKVAVLEAN
CCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEC
KIAWGATGRNGGQVTGSLSGDEAMTKQLRNCLGTDAEQYVWNMRWRGHEIIKERVNKYNI
EEEECCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHEECCCCCHHHHHHHHHHCCC
DCDLKFGHIHTAYKASHLKELEKTYSEGCKRGMEAELSMLNKEELSQHLNTPLYHGGLLN
EEEEEECCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCCCCCCCCCC
KRNMHLHSVNLCIGEARAAESLGALIFEHSAVVDINDGALVTVTTNKGKVVANSAVLAGN
CCCCEEEEEEEEECCHHHHHHHHHHHEECCEEEEECCCEEEEEECCCCEEEECCEEEECH
AYHKLARKKLRGMLFPASLGNCATAQLPAELARSLIPKDVAVYDTRFVLDYYRLTADNRL
HHHHHHHHHHHCEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHEEEHHEECCCCEE
MFGGGTNYSGRDSKNVALELRPAIERTFPQLKGVDIEFEWTGMAGIVLNRIPQLGKVSKN
EECCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCCCC
VFYCQGYSGHGVATSHIMGEVMASAVVGELQEFDLFAQMPHFRIPLNEWFGNQALALGMM
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCCHHHHHHHH
YYKVMENFR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]