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The map label for this gene is trmJ [H]

Identifier: 167623500

GI number: 167623500

Start: 1888444

End: 1889199

Strand: Direct

Name: trmJ [H]

Synonym: Shal_1569

Alternate gene names: 167623500

Gene position: 1888444-1889199 (Clockwise)

Preceding gene: 167623496

Following gene: 167623501

Centisome position: 36.13

GC content: 47.09

Gene sequence:

>756_bases
ATGCTCAGCAATATTCGCGTCGTTCTTGTCGGTACTTCCCACCCAGGGAATATAGGTTCTACAGCCCGTGCTATGAAAAC
CATGGGGTTATCCACTTTATATCTTGCTGAGCCAAAGGTTGAGCCGGATGGTCACTCGATCGCGTTAGCGGCGGGTGCAT
CGGATATTCTAAAGCATGCGATTACCGTAGGCTCTGTAGAAGAAGCCATTGCAGATTGTAGCTTAGTGATTGCAACCAGT
GCTCGTAGCCGCACGTTAGACTGGCCGATGCTTGAGCCACGTGAAGCGGGTGAGAAATTAGTTGGCTCGGCATTAACCGG
CCCTGTTGCTATCGTATTTGGACGTGAAAATAATGGCCTGACTAATGAAGAGCTGCAACGTTGCGATTATCACGTTGCTA
TTCCGGCTAACCCTGAATACACCTCTTTGAATCTGGCACAAGCAGTGCAGATTATCTGTTACGAGGCGCGAGTTGCGCAT
CTAGCTCAAGTAGAGAGCGGCGTTGAACCTGCACCTGTTGATGAAAATGATGAAGCGTATCCATCTTCAAGAGAGCGAGA
GAATTTCTTCGAGCATCTCGAAAATACGCTGTTGTCGACTAACTTTATCGTTAAGAATCACCCCGGCCAAATAATGACTA
AGTTACGTCGTTTATTCAGCCGTACCCGAATTGAGCGCCAAGAGATGAACATCTTGCGTGGTATATTGACCTCGATTGAT
AAAGTCGTTGACAAGACTGAGACTAAAGATAAGTAA

Upstream 100 bases:

>100_bases
CGATTTGACGGTTATATCAAATGCTGATTTTTGTATAAGCAAAATGAGTCGCTTGTGGTAACATTCTTTCACATGTTCTC
TCAGAAAATTAAGTAGTTTC

Downstream 100 bases:

>100_bases
GTAGCGCTAAAAAGGAAGTAGCTAAATGGGTGTTATCGCAAGACTGAAAGACGACATAGCCTCTATTTACCATAGAGATC
CAGCAGCAAACGGAACCATT

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPKVEPDGHSIALAAGASDILKHAITVGSVEEAIADCSLVIATS
ARSRTLDWPMLEPREAGEKLVGSALTGPVAIVFGRENNGLTNEELQRCDYHVAIPANPEYTSLNLAQAVQIICYEARVAH
LAQVESGVEPAPVDENDEAYPSSRERENFFEHLENTLLSTNFIVKNHPGQIMTKLRRLFSRTRIERQEMNILRGILTSID
KVVDKTETKDK

Sequences:

>Translated_251_residues
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPKVEPDGHSIALAAGASDILKHAITVGSVEEAIADCSLVIATS
ARSRTLDWPMLEPREAGEKLVGSALTGPVAIVFGRENNGLTNEELQRCDYHVAIPANPEYTSLNLAQAVQIICYEARVAH
LAQVESGVEPAPVDENDEAYPSSRERENFFEHLENTLLSTNFIVKNHPGQIMTKLRRLFSRTRIERQEMNILRGILTSID
KVVDKTETKDK
>Mature_251_residues
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPKVEPDGHSIALAAGASDILKHAITVGSVEEAIADCSLVIATS
ARSRTLDWPMLEPREAGEKLVGSALTGPVAIVFGRENNGLTNEELQRCDYHVAIPANPEYTSLNLAQAVQIICYEARVAH
LAQVESGVEPAPVDENDEAYPSSRERENFFEHLENTLLSTNFIVKNHPGQIMTKLRRLFSRTRIERQEMNILRGILTSID
KVVDKTETKDK

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=241, Percent_Identity=62.655601659751, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1790865, Length=197, Percent_Identity=35.0253807106599, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27503; Mature: 27503

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPKVEPDGHSIALAAGASDILKHA
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHHHHHH
ITVGSVEEAIADCSLVIATSARSRTLDWPMLEPREAGEKLVGSALTGPVAIVFGRENNGL
HHHCHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCC
TNEELQRCDYHVAIPANPEYTSLNLAQAVQIICYEARVAHLAQVESGVEPAPVDENDEAY
CHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PSSRERENFFEHLENTLLSTNFIVKNHPGQIMTKLRRLFSRTRIERQEMNILRGILTSID
CCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVVDKTETKDK
HHHHHHCCCCC
>Mature Secondary Structure
MLSNIRVVLVGTSHPGNIGSTARAMKTMGLSTLYLAEPKVEPDGHSIALAAGASDILKHA
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHHHHHH
ITVGSVEEAIADCSLVIATSARSRTLDWPMLEPREAGEKLVGSALTGPVAIVFGRENNGL
HHHCHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCC
TNEELQRCDYHVAIPANPEYTSLNLAQAVQIICYEARVAHLAQVESGVEPAPVDENDEAY
CHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PSSRERENFFEHLENTLLSTNFIVKNHPGQIMTKLRRLFSRTRIERQEMNILRGILTSID
CCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVVDKTETKDK
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA