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The map label for this gene is mak [H]

Identifier: 167623455

GI number: 167623455

Start: 1840113

End: 1841006

Strand: Direct

Name: mak [H]

Synonym: Shal_1524

Alternate gene names: 167623455

Gene position: 1840113-1841006 (Clockwise)

Preceding gene: 167623454

Following gene: 167623456

Centisome position: 35.2

GC content: 47.99

Gene sequence:

>894_bases
ATGTTGCGGATAGGCGTTGATCTTGGTGGGTCAAAAATAGAAGTTGTAGCACTTAACGAGCAGGGCAAAGAGTTATTTCG
CAAACGTCTTCAGACGCCAAGAGAATATAATGCCACTCTTGATGCGATAGAGCTTTTAGTCACTGAAGCCGAGCTGCAAT
TAGGGCAAAAGGGCACTGTGGGCGTTGGGATCCCCGGCGTTGTTTCACCATTTTCTGGCTTAGTTAAAAATGCCAATTCG
ACCTGGATTAACGGACACCCCCTCGATATCGACCTAGCTATGCGTTTAGACAGAAAAGTGAAAGTGGCAAATGATGCCAA
CTGTTTCGCGGTCTCAGAGGCTGTCGATGGCGCAGCAGCCGGAAAGGCCGTGGTATTTGGGGTGATTATTGGCACGGGTT
GTGGGGCTGGTATTGCCATTAACGGTAAAGTGCACGGTGGCAGTAATGGTATCGGCGGTGAATGGGGTCATAATCCTTTA
CCGTGGATGACGGCTGAAGAATTTAATTCAACTAACTGTTTTTGCGGCAATAAAGATTGTATTGAAACCTTTATCTCCGG
CACAGGCTTTGTGCGTGATTATAAGCTTGCTGGTGGTGATGCTAATAGCGGTATTGAAATCGCTTCACGTATGGAAGAGG
GCGACGCTCTCGCAACCGCGGCATTTGAACGCTATATCGATAGGCTTGCCCGTTCTTTAGCCCATATTATCAATGTACTG
GATCCCGATATTATCGTTTTGGGCGGTGGCGTATCCAATATTGAAGCTATTTATCCTTTGTTGCCAGAGGTATTACCCAA
ATATGTGTTAGGTGGAGAGTGTCGTACACCCGTGGTGCAAAATATGTATGGCAGCTCCTCTGGTGTGCGCGGCGCAGCTT
GGTTGTGGGGCTGA

Upstream 100 bases:

>100_bases
GGCTAGGGGAGAGTGCTGCGAACATTCGCGGGCTTAAAAAAGTTGTGCTTTGGTCGGATCTAAATATTCAAAAAATTAAT
AATAACTTTGGAGGGCAAAC

Downstream 100 bases:

>100_bases
CACAAGGATTTCAGCAGCAGTAATAAACCTTACATTTCTTACTGTTTTTTATTGGGAACTCAAATTTGAGAGGCGCTTAA
ATTCGAATGGCCACTGAATA

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLRIGVDLGGSKIEVVALNEQGKELFRKRLQTPREYNATLDAIELLVTEAELQLGQKGTVGVGIPGVVSPFSGLVKNANS
TWINGHPLDIDLAMRLDRKVKVANDANCFAVSEAVDGAAAGKAVVFGVIIGTGCGAGIAINGKVHGGSNGIGGEWGHNPL
PWMTAEEFNSTNCFCGNKDCIETFISGTGFVRDYKLAGGDANSGIEIASRMEEGDALATAAFERYIDRLARSLAHIINVL
DPDIIVLGGGVSNIEAIYPLLPEVLPKYVLGGECRTPVVQNMYGSSSGVRGAAWLWG

Sequences:

>Translated_297_residues
MLRIGVDLGGSKIEVVALNEQGKELFRKRLQTPREYNATLDAIELLVTEAELQLGQKGTVGVGIPGVVSPFSGLVKNANS
TWINGHPLDIDLAMRLDRKVKVANDANCFAVSEAVDGAAAGKAVVFGVIIGTGCGAGIAINGKVHGGSNGIGGEWGHNPL
PWMTAEEFNSTNCFCGNKDCIETFISGTGFVRDYKLAGGDANSGIEIASRMEEGDALATAAFERYIDRLARSLAHIINVL
DPDIIVLGGGVSNIEAIYPLLPEVLPKYVLGGECRTPVVQNMYGSSSGVRGAAWLWG
>Mature_297_residues
MLRIGVDLGGSKIEVVALNEQGKELFRKRLQTPREYNATLDAIELLVTEAELQLGQKGTVGVGIPGVVSPFSGLVKNANS
TWINGHPLDIDLAMRLDRKVKVANDANCFAVSEAVDGAAAGKAVVFGVIIGTGCGAGIAINGKVHGGSNGIGGEWGHNPL
PWMTAEEFNSTNCFCGNKDCIETFISGTGFVRDYKLAGGDANSGIEIASRMEEGDALATAAFERYIDRLARSLAHIINVL
DPDIIVLGGGVSNIEAIYPLLPEVLPKYVLGGECRTPVVQNMYGSSSGVRGAAWLWG

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=299, Percent_Identity=58.8628762541806, Blast_Score=373, Evalue=1e-105,
Organism=Escherichia coli, GI1787363, Length=300, Percent_Identity=41, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1790522, Length=301, Percent_Identity=27.2425249169435, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786891, Length=275, Percent_Identity=26.5454545454545, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787878, Length=266, Percent_Identity=22.1804511278195, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31287; Mature: 31287

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIGVDLGGSKIEVVALNEQGKELFRKRLQTPREYNATLDAIELLVTEAELQLGQKGTV
CEEEEEECCCCEEEEEEECHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCCCC
GVGIPGVVSPFSGLVKNANSTWINGHPLDIDLAMRLDRKVKVANDANCFAVSEAVDGAAA
CCCCCHHHHHHHHHHHCCCCCEECCCCCEEEHEEECCCEEEECCCCCEEEEHHHHCCCCC
GKAVVFGVIIGTGCGAGIAINGKVHGGSNGIGGEWGHNPLPWMTAEEFNSTNCFCGNKDC
CCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCEECCHHH
IETFISGTGFVRDYKLAGGDANSGIEIASRMEEGDALATAAFERYIDRLARSLAHIINVL
HHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
DPDIIVLGGGVSNIEAIYPLLPEVLPKYVLGGECRTPVVQNMYGSSSGVRGAAWLWG
CCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEECCC
>Mature Secondary Structure
MLRIGVDLGGSKIEVVALNEQGKELFRKRLQTPREYNATLDAIELLVTEAELQLGQKGTV
CEEEEEECCCCEEEEEEECHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCCCC
GVGIPGVVSPFSGLVKNANSTWINGHPLDIDLAMRLDRKVKVANDANCFAVSEAVDGAAA
CCCCCHHHHHHHHHHHCCCCCEECCCCCEEEHEEECCCEEEECCCCCEEEEHHHHCCCCC
GKAVVFGVIIGTGCGAGIAINGKVHGGSNGIGGEWGHNPLPWMTAEEFNSTNCFCGNKDC
CCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCEECCHHH
IETFISGTGFVRDYKLAGGDANSGIEIASRMEEGDALATAAFERYIDRLARSLAHIINVL
HHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
DPDIIVLGGGVSNIEAIYPLLPEVLPKYVLGGECRTPVVQNMYGSSSGVRGAAWLWG
CCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]