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The map label for this gene is spsK [H]

Identifier: 167623451

GI number: 167623451

Start: 1833063

End: 1834190

Strand: Direct

Name: spsK [H]

Synonym: Shal_1520

Alternate gene names: 167623451

Gene position: 1833063-1834190 (Clockwise)

Preceding gene: 167623450

Following gene: 167623453

Centisome position: 35.07

GC content: 48.32

Gene sequence:

>1128_bases
ATGCGAGTTCTTATCACCGGCGCAGCGGGTCAGCTGGGGCAGGCATTACTGTCGATAGCAGAGCTTACCCAAGTTACTGC
TGCAGAGCTAACCGCTCCTCAGCAAATGCTGGTGGCTTTGTTGCCAGAAGTCTTAGCGTGCATAGCAACCACTGATGAAG
TGATTGGCGTGTCTCATCAGCAGTTGGATATTTGTGCGCTTCACTCAATTCAAGCAGCCTTTGATGCTTTTAAGCCAGAT
GTAGTGATAAATTGCGCCGCCTTTAATGGTGTCGACAAGGCTGAGACAGATACCGACAAGGCGATTGCGGTGAATGCAAC
AGGGCCTAAGTTACTCGCTGGTGAGTGTAAGCGCCTAAATATTAGGTTAGTGCATATCTCGACTGACTTTGTGTTCGATG
GTGCGCTAAAGCGCCCATACACTGAGCAAGATATGCCATCGCCACTCTCAGCTTATGGTCGAAGTAAGCTCGAGGGCGAG
CGCTGGGTGAACGATATCTTAGGGGCTAAGGCAACGATTATACGTACCTCTTGGCTTTACAGTTGCTGCGGGCAGAACTT
TGTCAAAACCATGCAGGGGCTATTTAAGACAAGAGAGAGACTATCGGTTATAAATGATCAATCTGGTAGTCCAACCTGGT
GCGAAACCTTAGCCTTGGTGATTTTTAAGCTGATAAAGCAAACGCAGCTGGCTAATTCGGACAGGAAAGTTGCTGCACAA
AACCATAATGCAGACGGTACGGAAAAAGGACTGGCTCACTTATATCATTATGCAGCTGGCTCTTGCGTTGCTGAGGACTA
TCTCGTTGCAGAGGGCTTTAGCGCTGATGGCTCTCTCGTTGCTGAAGGCTCTAGCTGCTCTTGGTATGAGTTTGCTTGTG
AAATTCAGCGGTTGACGATGTCTGCTAATGCACAAATAGCAAAGGCTGAGGCGTGCCAAATTGAACCGATTAGCAGTTTA
TCTTGGCAGGCATTACATGAAAACCGTTTAGCACCAAGACCCGTGCAAAGCGCCTTGAATGCGCAGAAGGTGTGCCATCA
ACTTGGCATCAAGCCGGGAAGTCTAATTCGTGCGAGCTGGCAAGAGCAGTTACAAGCCATGATTAGCTATCAAAAGGTTA
AAGACTGA

Upstream 100 bases:

>100_bases
GCATTTTTGTCTGCAAGAGGTCATGCTTCCCTTGATGAATACTCTGTGCTGCTCGAAAGCTAAAACGAAAGCTAAAACTA
GAAGAGATAATTAAAGCAAG

Downstream 100 bases:

>100_bases
GAAGGTTCTAGGTCCTAGGAAAAGCATAGACAAAGCAGGTCCTAGCTAAAGGCTAAGACGGTACAAGCTGAGATGGAAAA
GCGGAACGGCCTGTGGCCTT

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MRVLITGAAGQLGQALLSIAELTQVTAAELTAPQQMLVALLPEVLACIATTDEVIGVSHQQLDICALHSIQAAFDAFKPD
VVINCAAFNGVDKAETDTDKAIAVNATGPKLLAGECKRLNIRLVHISTDFVFDGALKRPYTEQDMPSPLSAYGRSKLEGE
RWVNDILGAKATIIRTSWLYSCCGQNFVKTMQGLFKTRERLSVINDQSGSPTWCETLALVIFKLIKQTQLANSDRKVAAQ
NHNADGTEKGLAHLYHYAAGSCVAEDYLVAEGFSADGSLVAEGSSCSWYEFACEIQRLTMSANAQIAKAEACQIEPISSL
SWQALHENRLAPRPVQSALNAQKVCHQLGIKPGSLIRASWQEQLQAMISYQKVKD

Sequences:

>Translated_375_residues
MRVLITGAAGQLGQALLSIAELTQVTAAELTAPQQMLVALLPEVLACIATTDEVIGVSHQQLDICALHSIQAAFDAFKPD
VVINCAAFNGVDKAETDTDKAIAVNATGPKLLAGECKRLNIRLVHISTDFVFDGALKRPYTEQDMPSPLSAYGRSKLEGE
RWVNDILGAKATIIRTSWLYSCCGQNFVKTMQGLFKTRERLSVINDQSGSPTWCETLALVIFKLIKQTQLANSDRKVAAQ
NHNADGTEKGLAHLYHYAAGSCVAEDYLVAEGFSADGSLVAEGSSCSWYEFACEIQRLTMSANAQIAKAEACQIEPISSL
SWQALHENRLAPRPVQSALNAQKVCHQLGIKPGSLIRASWQEQLQAMISYQKVKD
>Mature_375_residues
MRVLITGAAGQLGQALLSIAELTQVTAAELTAPQQMLVALLPEVLACIATTDEVIGVSHQQLDICALHSIQAAFDAFKPD
VVINCAAFNGVDKAETDTDKAIAVNATGPKLLAGECKRLNIRLVHISTDFVFDGALKRPYTEQDMPSPLSAYGRSKLEGE
RWVNDILGAKATIIRTSWLYSCCGQNFVKTMQGLFKTRERLSVINDQSGSPTWCETLALVIFKLIKQTQLANSDRKVAAQ
NHNADGTEKGLAHLYHYAAGSCVAEDYLVAEGFSADGSLVAEGSSCSWYEFACEIQRLTMSANAQIAKAEACQIEPISSL
SWQALHENRLAPRPVQSALNAQKVCHQLGIKPGSLIRASWQEQLQAMISYQKVKD

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=184, Percent_Identity=33.1521739130435, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI11034825, Length=184, Percent_Identity=33.1521739130435, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1788352, Length=218, Percent_Identity=34.8623853211009, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 40712; Mature: 40712

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLITGAAGQLGQALLSIAELTQVTAAELTAPQQMLVALLPEVLACIATTDEVIGVSHQ
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHCCCCC
QLDICALHSIQAAFDAFKPDVVINCAAFNGVDKAETDTDKAIAVNATGPKLLAGECKRLN
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHCCCCEEE
IRLVHISTDFVFDGALKRPYTEQDMPSPLSAYGRSKLEGERWVNDILGAKATIIRTSWLY
EEEEEEEECEEECCHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHH
SCCGQNFVKTMQGLFKTRERLSVINDQSGSPTWCETLALVIFKLIKQTQLANSDRKVAAQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
NHNADGTEKGLAHLYHYAAGSCVAEDYLVAEGFSADGSLVAEGSSCSWYEFACEIQRLTM
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
SANAQIAKAEACQIEPISSLSWQALHENRLAPRPVQSALNAQKVCHQLGIKPGSLIRASW
CCCCCEECCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
QEQLQAMISYQKVKD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVLITGAAGQLGQALLSIAELTQVTAAELTAPQQMLVALLPEVLACIATTDEVIGVSHQ
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHCCCCC
QLDICALHSIQAAFDAFKPDVVINCAAFNGVDKAETDTDKAIAVNATGPKLLAGECKRLN
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHCCCCEEE
IRLVHISTDFVFDGALKRPYTEQDMPSPLSAYGRSKLEGERWVNDILGAKATIIRTSWLY
EEEEEEEECEEECCHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHH
SCCGQNFVKTMQGLFKTRERLSVINDQSGSPTWCETLALVIFKLIKQTQLANSDRKVAAQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
NHNADGTEKGLAHLYHYAAGSCVAEDYLVAEGFSADGSLVAEGSSCSWYEFACEIQRLTM
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
SANAQIAKAEACQIEPISSLSWQALHENRLAPRPVQSALNAQKVCHQLGIKPGSLIRASW
CCCCCEECCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
QEQLQAMISYQKVKD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]