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The map label for this gene is rfbB [H]

Identifier: 167623417

GI number: 167623417

Start: 1788071

End: 1789225

Strand: Direct

Name: rfbB [H]

Synonym: Shal_1486

Alternate gene names: 167623417

Gene position: 1788071-1789225 (Clockwise)

Preceding gene: 167623416

Following gene: 167623418

Centisome position: 34.21

GC content: 41.39

Gene sequence:

>1155_bases
ATGAAAATTTTAGTCACAGGTGGAGCCGGCTTTATTGGCTCTGCGGTAGTGCGTCATATCATCAATAATACACAAGATAG
TGTTATCAACGTCGATAAATTAACTTACGCAGGTAATCTAGAGTCGTTAGCAAGCATTGAGTCAAATGAACGTTATGTTT
TCGAACAAGTTGATATTTGTGACCGTGCGGAATTAGACAGAGTTTTTGTAAAGTGTAAGCCTGACGCTGTAATGCATTTA
GCTGCAGAGTCACATGTTGATCGCTCAATCACGGGCCCAGCAGATTTCATCCAGACAAATATCGTGGGCACATATACGCT
TCTTGAAGCGACACGAGCGTATTGGAACACATTGGCTGAAGATGCTAAGAAAGCATTTCGTTTTCATCATATTTCTACTG
ATGAAGTTTACGGCGACCTACCGCACCCTGATGATATTGAGGATGAAACAGAGCCCTCAACAGGCAAAGAGCCTTCAGCG
GATAATGAGCCTTCAGCCAATAAATCGCTACCTTTATTTACCGAAACTACATCATACGAACCCTCAAGCCCTTACTCTGC
ATCTAAGGCAAGTTCCGACCATCTTGTTCGTGCTTGGTTAAGAACCTATGGCCTACCAACCATAGTAACCAACTGTTCTA
ATAATTATGGTCCATATCACTTCCCTGAAAAGCTGATCCCATTAGTGATTTTAAATGCATTAGAGGGAAAACCATTACCT
ATCTACGGTAAAGGTGACCAAATCCGCGATTGGTTATATGTTGAAGATCACGCTAGAGCACTTTATAAAGTCGTTACCGA
AGGTGTCGTAGGCGAAACGTACAATATCGGTGGCCATAACGAAAAGCAAAATTTAGAAGTTGTTCAGACGATTTGTGCAA
TTTTAGATTCTTTAGTGCCAAAAGAAACAAAATATAAAGAACAAATTACTTATGTTACAGACAGGCCAGGTCACGACAGA
CGCTATGCAATTGATTCAAGTAAAATGCAACGTGAACTTGGTTGGACGCCGATCGAAACATTTGAAACAGGGCTTAAAAA
GACAATTGAATGGTATTTGACTAACCAAGAGTGGTGTCAACACGTGCAAGATGGGAGTTATCAGCGTGAACGTTTAGGTG
TAGTGCCATCTGCGACGAAGGAAGATGCACAATGA

Upstream 100 bases:

>100_bases
CCTCAACCTAGAGGCTGAGATTGGAAAGTCTGATAATTCAATTCACAACGTTTAAAATGAACTTGTTCTTAGGCAAGTAA
TACATCTTGGAAGCATAAAA

Downstream 100 bases:

>100_bases
AAGGTATTGTATTAGCTGGTGGCAGTGGTACTCGTTTATTTCCATTAACTCGTGGTGTATCAAAGCAACTGCTTCCAATC
TACGACAAGCCAATGGTGTT

Product: dTDP-glucose 4,6 dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLASIESNERYVFEQVDICDRAELDRVFVKCKPDAVMHL
AAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLAEDAKKAFRFHHISTDEVYGDLPHPDDIEDETEPSTGKEPSA
DNEPSANKSLPLFTETTSYEPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLP
IYGKGDQIRDWLYVEDHARALYKVVTEGVVGETYNIGGHNEKQNLEVVQTICAILDSLVPKETKYKEQITYVTDRPGHDR
RYAIDSSKMQRELGWTPIETFETGLKKTIEWYLTNQEWCQHVQDGSYQRERLGVVPSATKEDAQ

Sequences:

>Translated_384_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLASIESNERYVFEQVDICDRAELDRVFVKCKPDAVMHL
AAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLAEDAKKAFRFHHISTDEVYGDLPHPDDIEDETEPSTGKEPSA
DNEPSANKSLPLFTETTSYEPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLP
IYGKGDQIRDWLYVEDHARALYKVVTEGVVGETYNIGGHNEKQNLEVVQTICAILDSLVPKETKYKEQITYVTDRPGHDR
RYAIDSSKMQRELGWTPIETFETGLKKTIEWYLTNQEWCQHVQDGSYQRERLGVVPSATKEDAQ
>Mature_384_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLASIESNERYVFEQVDICDRAELDRVFVKCKPDAVMHL
AAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLAEDAKKAFRFHHISTDEVYGDLPHPDDIEDETEPSTGKEPSA
DNEPSANKSLPLFTETTSYEPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLP
IYGKGDQIRDWLYVEDHARALYKVVTEGVVGETYNIGGHNEKQNLEVVQTICAILDSLVPKETKYKEQITYVTDRPGHDR
RYAIDSSKMQRELGWTPIETFETGLKKTIEWYLTNQEWCQHVQDGSYQRERLGVVPSATKEDAQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=355, Percent_Identity=34.0845070422535, Blast_Score=204, Evalue=8e-53,
Organism=Homo sapiens, GI42516563, Length=359, Percent_Identity=22.8412256267409, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1788353, Length=368, Percent_Identity=74.4565217391304, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI48994969, Length=377, Percent_Identity=69.2307692307692, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1786974, Length=377, Percent_Identity=25.7294429708223, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=353, Percent_Identity=37.3937677053824, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=352, Percent_Identity=31.8181818181818, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI71982035, Length=371, Percent_Identity=27.7628032345013, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=373, Percent_Identity=27.6139410187668, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=353, Percent_Identity=24.0793201133144, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=386, Percent_Identity=25.9067357512953, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19923002, Length=383, Percent_Identity=26.1096605744125, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356223, Length=369, Percent_Identity=24.6612466124661, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 43163; Mature: 43163

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLASIESNERYVFEQVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHEEECCCHHHHHHCCCCCCEEEECCCCC
DRAELDRVFVKCKPDAVMHLAAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLAE
CHHHHHHEEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
DAKKAFRFHHISTDEVYGDLPHPDDIEDETEPSTGKEPSADNEPSANKSLPLFTETTSYE
HHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCC
PSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLP
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCE
IYGKGDQIRDWLYVEDHARALYKVVTEGVVGETYNIGGHNEKQNLEVVQTICAILDSLVP
EECCCCCCCCEEEEHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCC
KETKYKEQITYVTDRPGHDRRYAIDSSKMQRELGWTPIETFETGLKKTIEWYLTNQEWCQ
CCHHHHHHEEEEECCCCCCCEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHEECCHHHHH
HVQDGSYQRERLGVVPSATKEDAQ
HHHCCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLASIESNERYVFEQVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHEEECCCHHHHHHCCCCCCEEEECCCCC
DRAELDRVFVKCKPDAVMHLAAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLAE
CHHHHHHEEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
DAKKAFRFHHISTDEVYGDLPHPDDIEDETEPSTGKEPSADNEPSANKSLPLFTETTSYE
HHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCC
PSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLP
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCE
IYGKGDQIRDWLYVEDHARALYKVVTEGVVGETYNIGGHNEKQNLEVVQTICAILDSLVP
EECCCCCCCCEEEEHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCC
KETKYKEQITYVTDRPGHDRRYAIDSSKMQRELGWTPIETFETGLKKTIEWYLTNQEWCQ
CCHHHHHHEEEEECCCCCCCEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHEECCHHHHH
HVQDGSYQRERLGVVPSATKEDAQ
HHHCCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]