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The map label for this gene is fdnI [H]

Identifier: 167622435

GI number: 167622435

Start: 596790

End: 597434

Strand: Direct

Name: fdnI [H]

Synonym: Shal_0495

Alternate gene names: 167622435

Gene position: 596790-597434 (Clockwise)

Preceding gene: 167622434

Following gene: 167622436

Centisome position: 11.42

GC content: 47.13

Gene sequence:

>645_bases
ATGCATAAGCAGAATATGATTTTGCGTCATAAGTTGTTCGACCGCATCTGTCACTGGTTTGTGGTTGCCGCAGGTTTGGT
GACATTCCTAACAGGCTTTGCATTCTTCTTCCCATCATTTCAATGGTTGGGATCGATTGCAGGTACGCCACAGATGGCGC
GTTTTGTTCATCCGATTGCCGGTTTGCTGATGTGCTTACCGCTAATGCTGATGCTAGTGCGCTATTACCACCATAACAAG
TGGGAAAAGAATGACCTGACATGGATGTTAGCGATTAAGGATGTGATGTTCGAAAACGAGGACAAGATCCCCGCTATCGG
CCATTACAACCCAGGTCAAAAAGTGCTGTTCCGCACCTTCGTGGTGACCTCAATCGGCTTGACGATTACTGGTTTCATGA
TGTGGCAGCCTTACTTTGCCCCACACTTCGCGGCTATAACAATCCAGTGGGCCATTTTGATCCACGCAATCTGCGCAGTG
ATCATGTTGATCTTTGTGATTGTGCATTTTTGGATGGCCACTTGGGTGGAAGGATCGGTTGCTGGCATGTTGTACGGCTA
TGTGTCGCGGGCTTGGTGCAAGAAGCATCACCCTAACATGCTAAACGATCCTGAATTAAAGACTAATAAGCAGGAAAAAC
ACTAA

Upstream 100 bases:

>100_bases
GTCTACACCGTATCACTGTGGGCCGTAATATCGTCGAAGAAGATGAGCCGGCAGCGTTTGACCACACTCACTCAGACAAG
TCAGATAAGGAGTAACACTC

Downstream 100 bases:

>100_bases
TTATGAGCACAGCAATACTCGACGCCGAAAAGATCCCAACTCAGTCATTGGAGATCAAAACCGTGATCCCTGCTGATCCC
AAAGCCGTTTATCAATTTAG

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase cytochrome b556 subunit; Formate dehydrogenase-N subunit gamma; FDH-N subunit gamma [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MHKQNMILRHKLFDRICHWFVVAAGLVTFLTGFAFFFPSFQWLGSIAGTPQMARFVHPIAGLLMCLPLMLMLVRYYHHNK
WEKNDLTWMLAIKDVMFENEDKIPAIGHYNPGQKVLFRTFVVTSIGLTITGFMMWQPYFAPHFAAITIQWAILIHAICAV
IMLIFVIVHFWMATWVEGSVAGMLYGYVSRAWCKKHHPNMLNDPELKTNKQEKH

Sequences:

>Translated_214_residues
MHKQNMILRHKLFDRICHWFVVAAGLVTFLTGFAFFFPSFQWLGSIAGTPQMARFVHPIAGLLMCLPLMLMLVRYYHHNK
WEKNDLTWMLAIKDVMFENEDKIPAIGHYNPGQKVLFRTFVVTSIGLTITGFMMWQPYFAPHFAAITIQWAILIHAICAV
IMLIFVIVHFWMATWVEGSVAGMLYGYVSRAWCKKHHPNMLNDPELKTNKQEKH
>Mature_214_residues
MHKQNMILRHKLFDRICHWFVVAAGLVTFLTGFAFFFPSFQWLGSIAGTPQMARFVHPIAGLLMCLPLMLMLVRYYHHNK
WEKNDLTWMLAIKDVMFENEDKIPAIGHYNPGQKVLFRTFVVTSIGLTITGFMMWQPYFAPHFAAITIQWAILIHAICAV
IMLIFVIVHFWMATWVEGSVAGMLYGYVSRAWCKKHHPNMLNDPELKTNKQEKH

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. Subunit gamma is the cytochrome b556(FDN) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787750, Length=214, Percent_Identity=42.5233644859813, Blast_Score=186, Evalue=7e-49,
Organism=Escherichia coli, GI1790325, Length=213, Percent_Identity=35.2112676056338, Blast_Score=168, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24928; Mature: 24928

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
6.5 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKQNMILRHKLFDRICHWFVVAAGLVTFLTGFAFFFPSFQWLGSIAGTPQMARFVHPIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GLLMCLPLMLMLVRYYHHNKWEKNDLTWMLAIKDVMFENEDKIPAIGHYNPGQKVLFRTF
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
VVTSIGLTITGFMMWQPYFAPHFAAITIQWAILIHAICAVIMLIFVIVHFWMATWVEGSV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
AGMLYGYVSRAWCKKHHPNMLNDPELKTNKQEKH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHKQNMILRHKLFDRICHWFVVAAGLVTFLTGFAFFFPSFQWLGSIAGTPQMARFVHPIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GLLMCLPLMLMLVRYYHHNKWEKNDLTWMLAIKDVMFENEDKIPAIGHYNPGQKVLFRTF
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
VVTSIGLTITGFMMWQPYFAPHFAAITIQWAILIHAICAVIMLIFVIVHFWMATWVEGSV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
AGMLYGYVSRAWCKKHHPNMLNDPELKTNKQEKH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]