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The map label for this gene is fdoH [H]

Identifier: 167622434

GI number: 167622434

Start: 595882

End: 596784

Strand: Direct

Name: fdoH [H]

Synonym: Shal_0494

Alternate gene names: 167622434

Gene position: 595882-596784 (Clockwise)

Preceding gene: 167622433

Following gene: 167622435

Centisome position: 11.4

GC content: 48.84

Gene sequence:

>903_bases
ATGTCTGCTCAAGATATTATTTTAAGCTCTGGTACATCAGAGCTCACCCCTGCTGCGCAGGTACGCCGCAAATTAAGCAA
GGTGGCCAAGTTATTTGATGCCACTAAGTGTAGCGGCTGTAAAGCTTGTCAGGTTTCCTGTTCTGAATGGAATGACCTCA
GAGCGCCAGTCGGCAGCTTCCAAGGTAGTTACCAGAACCCAATGGATCTGTCTTCAGAGTGCTGGACACTGATGAAGTAC
AACGAAATTGAGCAGGACAACAAGTTACGCTGGAACTTTACTCATAGTGCTTGCATGCATTGTGCTGATCCAGCCTGCTT
AACGGCTTGCTCGACTAAAGGGGCGATTGTTCAGCGTGCTAACGGCGTTGTCGATTTTGAATCTGATAAGTGTACGGGTT
GTGGTTACTGTGTATCGGCTTGTCCGTTCGATGTACCAAGACTCGACCCTATCGATCAAAAAGCCTACAAGTGCAGCATG
TGCTCTGACCGTCTACAAGTTGGTCAAGAGCCAGCGTGTGTGAAGTCTTGTGTTACTGGGGCGCTTAAATACGGTACGCG
TGAAGATATGTTGTTTATTGCAGACCTTCGAATTAAAGAACTGCATAAACAAGGCTTCACTAAAGCGGGTCTGTACAGTC
CTGAGGGCGTGGGTGGCACTGGCATGATGATGATCTTGCATGATGTTAGCCAGCCTGAAAACTATGGCTTACCAGCCGAT
CCAAAAACAAGCGTGCCAGTGAAACTGTGGCAAGACGTGGTTAAGCCATTAGGTACAGCGGGTATTCTCGCGACCGTGGC
GGTGGCCTGTCTACACCGTATCACTGTGGGCCGTAATATCGTCGAAGAAGATGAGCCGGCAGCGTTTGACCACACTCACT
CAGACAAGTCAGATAAGGAGTAA

Upstream 100 bases:

>100_bases
GCTGTAACGTACTGACCACTGAAGTGGGTGATGCTAATACTCAAGCACCTGAATATAAAGCGTTCCTAGTGGATATTACT
AAGGCTGAGGGAGTGTAATC

Downstream 100 bases:

>100_bases
CACTCATGCATAAGCAGAATATGATTTTGCGTCATAAGTTGTTCGACCGCATCTGTCACTGGTTTGTGGTTGCCGCAGGT
TTGGTGACATTCCTAACAGG

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSAQDIILSSGTSELTPAAQVRRKLSKVAKLFDATKCSGCKACQVSCSEWNDLRAPVGSFQGSYQNPMDLSSECWTLMKY
NEIEQDNKLRWNFTHSACMHCADPACLTACSTKGAIVQRANGVVDFESDKCTGCGYCVSACPFDVPRLDPIDQKAYKCSM
CSDRLQVGQEPACVKSCVTGALKYGTREDMLFIADLRIKELHKQGFTKAGLYSPEGVGGTGMMMILHDVSQPENYGLPAD
PKTSVPVKLWQDVVKPLGTAGILATVAVACLHRITVGRNIVEEDEPAAFDHTHSDKSDKE

Sequences:

>Translated_300_residues
MSAQDIILSSGTSELTPAAQVRRKLSKVAKLFDATKCSGCKACQVSCSEWNDLRAPVGSFQGSYQNPMDLSSECWTLMKY
NEIEQDNKLRWNFTHSACMHCADPACLTACSTKGAIVQRANGVVDFESDKCTGCGYCVSACPFDVPRLDPIDQKAYKCSM
CSDRLQVGQEPACVKSCVTGALKYGTREDMLFIADLRIKELHKQGFTKAGLYSPEGVGGTGMMMILHDVSQPENYGLPAD
PKTSVPVKLWQDVVKPLGTAGILATVAVACLHRITVGRNIVEEDEPAAFDHTHSDKSDKE
>Mature_299_residues
SAQDIILSSGTSELTPAAQVRRKLSKVAKLFDATKCSGCKACQVSCSEWNDLRAPVGSFQGSYQNPMDLSSECWTLMKYN
EIEQDNKLRWNFTHSACMHCADPACLTACSTKGAIVQRANGVVDFESDKCTGCGYCVSACPFDVPRLDPIDQKAYKCSMC
SDRLQVGQEPACVKSCVTGALKYGTREDMLFIADLRIKELHKQGFTKAGLYSPEGVGGTGMMMILHDVSQPENYGLPADP
KTSVPVKLWQDVVKPLGTAGILATVAVACLHRITVGRNIVEEDEPAAFDHTHSDKSDKE

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=298, Percent_Identity=51.3422818791946, Blast_Score=337, Evalue=8e-94,
Organism=Escherichia coli, GI1787749, Length=287, Percent_Identity=52.9616724738676, Blast_Score=335, Evalue=2e-93,
Organism=Escherichia coli, GI1789370, Length=307, Percent_Identity=28.9902280130293, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI226510944, Length=185, Percent_Identity=31.8918918918919, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787122, Length=165, Percent_Identity=32.7272727272727, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787872, Length=165, Percent_Identity=32.7272727272727, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI2367345, Length=181, Percent_Identity=29.8342541436464, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787963, Length=155, Percent_Identity=25.1612903225806, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787478, Length=88, Percent_Identity=36.3636363636364, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787740, Length=105, Percent_Identity=37.1428571428571, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 32649; Mature: 32517

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
6.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQDIILSSGTSELTPAAQVRRKLSKVAKLFDATKCSGCKACQVSCSEWNDLRAPVGSF
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHCCHHHC
QGSYQNPMDLSSECWTLMKYNEIEQDNKLRWNFTHSACMHCADPACLTACSTKGAIVQRA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCHHHHHHHCCCCHHHHHC
NGVVDFESDKCTGCGYCVSACPFDVPRLDPIDQKAYKCSMCSDRLQVGQEPACVKSCVTG
CCCEEECCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALKYGTREDMLFIADLRIKELHKQGFTKAGLYSPEGVGGTGMMMILHDVSQPENYGLPAD
HHHCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEEHHCCCCCCCCCCCC
PKTSVPVKLWQDVVKPLGTAGILATVAVACLHRITVGRNIVEEDEPAAFDHTHSDKSDKE
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAQDIILSSGTSELTPAAQVRRKLSKVAKLFDATKCSGCKACQVSCSEWNDLRAPVGSF
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHCCHHHC
QGSYQNPMDLSSECWTLMKYNEIEQDNKLRWNFTHSACMHCADPACLTACSTKGAIVQRA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCHHHHHHHCCCCHHHHHC
NGVVDFESDKCTGCGYCVSACPFDVPRLDPIDQKAYKCSMCSDRLQVGQEPACVKSCVTG
CCCEEECCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALKYGTREDMLFIADLRIKELHKQGFTKAGLYSPEGVGGTGMMMILHDVSQPENYGLPAD
HHHCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEEHHCCCCCCCCCCCC
PKTSVPVKLWQDVVKPLGTAGILATVAVACLHRITVGRNIVEEDEPAAFDHTHSDKSDKE
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]