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The map label for this gene is rsmD [H]

Identifier: 167622227

GI number: 167622227

Start: 342294

End: 342914

Strand: Reverse

Name: rsmD [H]

Synonym: Shal_0286

Alternate gene names: 167622227

Gene position: 342914-342294 (Counterclockwise)

Preceding gene: 167622232

Following gene: 167622226

Centisome position: 6.56

GC content: 49.6

Gene sequence:

>621_bases
ATGGTCAAAAAAAGACCTTCTAGCGGTCAAGTTAGGATTATTTCAGGCCAGTGGCGCTCTCGAAAGCTGCCCATTCACGA
CCTTGAAGGGCTGCGCCCAACCACAGACCGTGTCAGAGAGACTCTGTTCAACTGGGTTGCCAATGACATTCGCGGTGCTC
GCGTACTCGACTGCTTTGCCGGTAGCGGAGCGTTAAGTCTTGAGTCACTGTCACGATATGCCAGTTACGCCAAGATATTT
GAGCTACAAGTCCAAGCCGCTAAGCAGTTGGTAGCTAACTTAGCCACGCTAAAGTGCGATAACGCCGATGTGATCAAGGG
TGACAGCCTAGCTCTGCTGGCTAATAAGCCCAGCGAAGGCTTCGATCTGGTCTTTATCGACCCACCTTTTAGAAAGTCAC
TAGCCGAGAAAAGCATTAGCCTTATCGATCAGCACCAATGGCTTAACGACGATGCGCTTATCTACGTAGAAACCGAGAGT
GAAATCGCGATGCTAACCGTGCCAGGGAGCTGGAGTTTGCTTAAAGAGAAAAAGGCTGGCCAAGTGATCTACCGTTTATA
TCAATATTCAGCCGAGTCTGCGCTCGATGAAACGACATGTGACACCGAGGAAACCAATTAA

Upstream 100 bases:

>100_bases
GCTTTTTACCTTTTAGCTGTGCTTTGATATAAAATAACGACCTAAATTCAGACGCCATAGTCTACCACTTTCTTTCATTA
GGCAAAATCACGAGGACACC

Downstream 100 bases:

>100_bases
ATGAAAGCTATGCTAGTTCTAGGTAAAATTGTTACCGCATTCGCTTGGTTAATGATGCTATATAATTTAGTTTCGCCTTT
TGCAGGCAATATTGCGGTGA

Product: putative methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MVKKRPSSGQVRIISGQWRSRKLPIHDLEGLRPTTDRVRETLFNWVANDIRGARVLDCFAGSGALSLESLSRYASYAKIF
ELQVQAAKQLVANLATLKCDNADVIKGDSLALLANKPSEGFDLVFIDPPFRKSLAEKSISLIDQHQWLNDDALIYVETES
EIAMLTVPGSWSLLKEKKAGQVIYRLYQYSAESALDETTCDTEETN

Sequences:

>Translated_206_residues
MVKKRPSSGQVRIISGQWRSRKLPIHDLEGLRPTTDRVRETLFNWVANDIRGARVLDCFAGSGALSLESLSRYASYAKIF
ELQVQAAKQLVANLATLKCDNADVIKGDSLALLANKPSEGFDLVFIDPPFRKSLAEKSISLIDQHQWLNDDALIYVETES
EIAMLTVPGSWSLLKEKKAGQVIYRLYQYSAESALDETTCDTEETN
>Mature_206_residues
MVKKRPSSGQVRIISGQWRSRKLPIHDLEGLRPTTDRVRETLFNWVANDIRGARVLDCFAGSGALSLESLSRYASYAKIF
ELQVQAAKQLVANLATLKCDNADVIKGDSLALLANKPSEGFDLVFIDPPFRKSLAEKSISLIDQHQWLNDDALIYVETES
EIAMLTVPGSWSLLKEKKAGQVIYRLYQYSAESALDETTCDTEETN

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=196, Percent_Identity=56.1224489795918, Blast_Score=224, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 23025; Mature: 23025

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKRPSSGQVRIISGQWRSRKLPIHDLEGLRPTTDRVRETLFNWVANDIRGARVLDCFA
CCCCCCCCCCEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
GSGALSLESLSRYASYAKIFELQVQAAKQLVANLATLKCDNADVIKGDSLALLANKPSEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCCCEEEEECCCCCC
FDLVFIDPPFRKSLAEKSISLIDQHQWLNDDALIYVETESEIAMLTVPGSWSLLKEKKAG
CEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHH
QVIYRLYQYSAESALDETTCDTEETN
HHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVKKRPSSGQVRIISGQWRSRKLPIHDLEGLRPTTDRVRETLFNWVANDIRGARVLDCFA
CCCCCCCCCCEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
GSGALSLESLSRYASYAKIFELQVQAAKQLVANLATLKCDNADVIKGDSLALLANKPSEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCCCEEEEECCCCCC
FDLVFIDPPFRKSLAEKSISLIDQHQWLNDDALIYVETESEIAMLTVPGSWSLLKEKKAG
CEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHH
QVIYRLYQYSAESALDETTCDTEETN
HHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]