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The map label for this gene is pleD [H]

Identifier: 167622217

GI number: 167622217

Start: 331128

End: 332306

Strand: Reverse

Name: pleD [H]

Synonym: Shal_0276

Alternate gene names: 167622217

Gene position: 332306-331128 (Counterclockwise)

Preceding gene: 167622219

Following gene: 167622214

Centisome position: 6.36

GC content: 44.11

Gene sequence:

>1179_bases
GTGCCAGTATTAGAGCGAAGCCTACGTTATCTCTTTGCCCCAATTGTTCTTACGTTCATTGGTATCGCCGCGCTCCAGCA
GCTAACGGCAACATGGCAGGAGTGGCTACCGAGTTTTCACGAACTCCCCTACTACTTGCTAGCGCTTTCTACACTGCTGG
CTCTGCAATTTAACTGTGGCCGCATTAGCTACCTCTCGCTATTGTTGCTGCTTTATTACTATCTTAGGCAAGTCGCTCCG
CTTGCTGCATTAGACCAGGATCAAGTCTTTCTTGCTGGTACCCTAATCATCACCTTTTTTGCTATCAGCAAGGATAGAAG
TCCATTTTCTATCCATTTTTTTAAGGTATTACTTGGGATCTCCCTTTGCTTTGCGCTAGCCTTTGTCTGGAAGGCCAGTG
CCTCTGGACTAGCATCATTTAACATTACTCAATTACCCAGTATGCTTAACTTGGTTGCCTCCCTGTATATTCCCGTCGGG
ATCTCATGCATAGTGATTAGCATCTATACTGTTTTGCACGCATCCGGAACTAACTCAACTATTGCGCTGACACAGTTTAT
CTGGCTATTTCATCACTACCTTCCCAAAGAAATGCCACTTGCCATCTTGCTATCGATGCTGGCAACTGCGTACTTATTCT
CTATGCTTTTCGCGCTCTATGATCTCGCCTATAAAGATGAGTTAACCGGACTATCCTCCCGACGCGCCTTAAATACCATG
GCGTTATCACTCAATAACCATTACAGCCTCGCCATGATTGATATCGATCATTTCAAAAAATTTAACGACACCTATGGCCA
CGATGTGGGTGATCAAGTACTGCGCTTAGTGGCAAAAAAACTCAGTAAAGTAAAAGGCGGTGGACAAGTCTTTCGCTACG
GCGGTGAGGAATTTACCATTGTGTTTGAAAATAAAAATATTGAACGAGTGCTACCTCACCTAGAGGAATTAAGGCATAAA
ATCAGTCACTACGATATTGTACTACGCGAGCAGGGGCGAAAAAGTAGGAGTAAATTAGATAGGAACAGCAAGCATAAGGC
GAACCAGGCGCTAAATATCACCATTTCAATTGGTGTCGCCGAACAGCATGGTGAGACCACATTTGAAAAAACATTAAAAC
GCGCTGATGAGGCGTTATACAAAGCTAAGAATAACGGTCGAAACTGTGTTTTTGCCTAA

Upstream 100 bases:

>100_bases
AACGATGACCTAGCATTAATTTCATGGGGGAGGACAACAAAGCTCGGCTATTGATAATGAGCTATAATCTAGATCTAGCC
GTTATCAAGGAGGATTGGGA

Downstream 100 bases:

>100_bases
GCTAAAAACAAGCAAACTGAATAAGAGATTAGATTAAGATAACCCAAAGCTTTATAGGCTTGGGGTTATCTTAGTGAGAC
TAGATTAGATGGAGTAGAAG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MPVLERSLRYLFAPIVLTFIGIAALQQLTATWQEWLPSFHELPYYLLALSTLLALQFNCGRISYLSLLLLLYYYLRQVAP
LAALDQDQVFLAGTLIITFFAISKDRSPFSIHFFKVLLGISLCFALAFVWKASASGLASFNITQLPSMLNLVASLYIPVG
ISCIVISIYTVLHASGTNSTIALTQFIWLFHHYLPKEMPLAILLSMLATAYLFSMLFALYDLAYKDELTGLSSRRALNTM
ALSLNNHYSLAMIDIDHFKKFNDTYGHDVGDQVLRLVAKKLSKVKGGGQVFRYGGEEFTIVFENKNIERVLPHLEELRHK
ISHYDIVLREQGRKSRSKLDRNSKHKANQALNITISIGVAEQHGETTFEKTLKRADEALYKAKNNGRNCVFA

Sequences:

>Translated_392_residues
MPVLERSLRYLFAPIVLTFIGIAALQQLTATWQEWLPSFHELPYYLLALSTLLALQFNCGRISYLSLLLLLYYYLRQVAP
LAALDQDQVFLAGTLIITFFAISKDRSPFSIHFFKVLLGISLCFALAFVWKASASGLASFNITQLPSMLNLVASLYIPVG
ISCIVISIYTVLHASGTNSTIALTQFIWLFHHYLPKEMPLAILLSMLATAYLFSMLFALYDLAYKDELTGLSSRRALNTM
ALSLNNHYSLAMIDIDHFKKFNDTYGHDVGDQVLRLVAKKLSKVKGGGQVFRYGGEEFTIVFENKNIERVLPHLEELRHK
ISHYDIVLREQGRKSRSKLDRNSKHKANQALNITISIGVAEQHGETTFEKTLKRADEALYKAKNNGRNCVFA
>Mature_391_residues
PVLERSLRYLFAPIVLTFIGIAALQQLTATWQEWLPSFHELPYYLLALSTLLALQFNCGRISYLSLLLLLYYYLRQVAPL
AALDQDQVFLAGTLIITFFAISKDRSPFSIHFFKVLLGISLCFALAFVWKASASGLASFNITQLPSMLNLVASLYIPVGI
SCIVISIYTVLHASGTNSTIALTQFIWLFHHYLPKEMPLAILLSMLATAYLFSMLFALYDLAYKDELTGLSSRRALNTMA
LSLNNHYSLAMIDIDHFKKFNDTYGHDVGDQVLRLVAKKLSKVKGGGQVFRYGGEEFTIVFENKNIERVLPHLEELRHKI
SHYDIVLREQGRKSRSKLDRNSKHKANQALNITISIGVAEQHGETTFEKTLKRADEALYKAKNNGRNCVFA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=178, Percent_Identity=36.5168539325843, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI145693134, Length=173, Percent_Identity=34.6820809248555, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787056, Length=176, Percent_Identity=33.5227272727273, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787262, Length=188, Percent_Identity=32.9787234042553, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786584, Length=176, Percent_Identity=29.5454545454545, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787802, Length=173, Percent_Identity=31.2138728323699, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787816, Length=232, Percent_Identity=30.6034482758621, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87082007, Length=180, Percent_Identity=30.5555555555556, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081977, Length=184, Percent_Identity=32.0652173913043, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44316; Mature: 44185

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLERSLRYLFAPIVLTFIGIAALQQLTATWQEWLPSFHELPYYLLALSTLLALQFNCG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
RISYLSLLLLLYYYLRQVAPLAALDQDQVFLAGTLIITFFAISKDRSPFSIHFFKVLLGI
HHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SLCFALAFVWKASASGLASFNITQLPSMLNLVASLYIPVGISCIVISIYTVLHASGTNST
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
IALTQFIWLFHHYLPKEMPLAILLSMLATAYLFSMLFALYDLAYKDELTGLSSRRALNTM
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALSLNNHYSLAMIDIDHFKKFNDTYGHDVGDQVLRLVAKKLSKVKGGGQVFRYGGEEFTI
HEEECCCEEEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEE
VFENKNIERVLPHLEELRHKISHYDIVLREQGRKSRSKLDRNSKHKANQALNITISIGVA
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHCCCCEEEEEEEEEEC
EQHGETTFEKTLKRADEALYKAKNNGRNCVFA
HHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PVLERSLRYLFAPIVLTFIGIAALQQLTATWQEWLPSFHELPYYLLALSTLLALQFNCG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
RISYLSLLLLLYYYLRQVAPLAALDQDQVFLAGTLIITFFAISKDRSPFSIHFFKVLLGI
HHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SLCFALAFVWKASASGLASFNITQLPSMLNLVASLYIPVGISCIVISIYTVLHASGTNST
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
IALTQFIWLFHHYLPKEMPLAILLSMLATAYLFSMLFALYDLAYKDELTGLSSRRALNTM
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALSLNNHYSLAMIDIDHFKKFNDTYGHDVGDQVLRLVAKKLSKVKGGGQVFRYGGEEFTI
HEEECCCEEEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEE
VFENKNIERVLPHLEELRHKISHYDIVLREQGRKSRSKLDRNSKHKANQALNITISIGVA
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHCCCCEEEEEEEEEEC
EQHGETTFEKTLKRADEALYKAKNNGRNCVFA
HHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA