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The map label for this gene is bioH [H]

Identifier: 167622185

GI number: 167622185

Start: 292982

End: 293752

Strand: Reverse

Name: bioH [H]

Synonym: Shal_0244

Alternate gene names: 167622185

Gene position: 293752-292982 (Counterclockwise)

Preceding gene: 167622187

Following gene: 167622184

Centisome position: 5.62

GC content: 49.81

Gene sequence:

>771_bases
GTGAGCCAAATAAAGTTACATGTTGAATCTATCGGCCAAGGCCCTGACGTCGTCATGCTCCATGGTTGGGGGGTTAATAG
CGCCGTATTTACTCCACTTCATAGTGAATTAAACAATTATAGGTTCCACTATGTCGATCTGCCGGGATTTGGCCATAGCC
AGGCCGTTATGGGCAATATTGAACATTGGCTTGAAGCTATTATGGAACAGGTACCTGAGAAGGCTATTTGGACTGGGTGG
TCACTGGGGGGGCTGGTTGCCACTCTGGCAGCAATAAAGCAGCCACAGCGTGTATCGGCCCTATGCACTATCGCGTCATC
GCCTTGTTTTATGGCTCGTGAAGATTGGCCGGGGATCCCTGCCGAGGTGTTAGGCCAGTTTGCCAGCCAACTGACTCAAG
ATCTCGATAAAACGGTCGAGCGCTTCTTGGCGATTCAAGCCATGGGCAGCCAAACGGCTAAGGCCGATATTAAACAGTTA
CGTGAATTAGTACTGGCTCGTCCCAGCGCACAGCAACCAGCACTCGCTCAAGGCTTGGACATGCTTGAAAATGTCGACTT
ACGTTCGCAGCTATCTCAAATAGATCAACCCTGGTTGAGAGTGTGGGGACGATTAGACGGCCTAGTGCCTCGCAGAGTCA
TTAAGGCAATGCCACAGGTTTCTGCCAGTGAAGACATGCTGCTTACCAAAGCCTCTCACGCTCCGTTCATTAGTCATCAA
GCAGAGTTTCTCGATGGTTACAAACTTTGGCTAGATAAGATAAGCAACTGA

Upstream 100 bases:

>100_bases
TAATAGGTGAAATCCTTTTCAATATTCCCCTGTTAACCAAAGACAACACGAATTAGGTAACCCCTTTTCTACGTTGCCTC
GTTAAGCTATGAGGTCCTAA

Downstream 100 bases:

>100_bases
GAAATCGATACCAAGCGTTAAGGTGAGTCTGACCATGGCGCTTTATTTTTACGCTGAATTAGGTTAAGATTTAACCACAC
TTTTCAGCGGGAAATATTAT

Product: bioH protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSQIKLHVESIGQGPDVVMLHGWGVNSAVFTPLHSELNNYRFHYVDLPGFGHSQAVMGNIEHWLEAIMEQVPEKAIWTGW
SLGGLVATLAAIKQPQRVSALCTIASSPCFMAREDWPGIPAEVLGQFASQLTQDLDKTVERFLAIQAMGSQTAKADIKQL
RELVLARPSAQQPALAQGLDMLENVDLRSQLSQIDQPWLRVWGRLDGLVPRRVIKAMPQVSASEDMLLTKASHAPFISHQ
AEFLDGYKLWLDKISN

Sequences:

>Translated_256_residues
MSQIKLHVESIGQGPDVVMLHGWGVNSAVFTPLHSELNNYRFHYVDLPGFGHSQAVMGNIEHWLEAIMEQVPEKAIWTGW
SLGGLVATLAAIKQPQRVSALCTIASSPCFMAREDWPGIPAEVLGQFASQLTQDLDKTVERFLAIQAMGSQTAKADIKQL
RELVLARPSAQQPALAQGLDMLENVDLRSQLSQIDQPWLRVWGRLDGLVPRRVIKAMPQVSASEDMLLTKASHAPFISHQ
AEFLDGYKLWLDKISN
>Mature_255_residues
SQIKLHVESIGQGPDVVMLHGWGVNSAVFTPLHSELNNYRFHYVDLPGFGHSQAVMGNIEHWLEAIMEQVPEKAIWTGWS
LGGLVATLAAIKQPQRVSALCTIASSPCFMAREDWPGIPAEVLGQFASQLTQDLDKTVERFLAIQAMGSQTAKADIKQLR
ELVLARPSAQQPALAQGLDMLENVDLRSQLSQIDQPWLRVWGRLDGLVPRRVIKAMPQVSASEDMLLTKASHAPFISHQA
EFLDGYKLWLDKISN

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=239, Percent_Identity=48.9539748953975, Blast_Score=231, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 28414; Mature: 28283

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIKLHVESIGQGPDVVMLHGWGVNSAVFTPLHSELNNYRFHYVDLPGFGHSQAVMGNI
CCCEEEEHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
EHWLEAIMEQVPEKAIWTGWSLGGLVATLAAIKQPQRVSALCTIASSPCFMAREDWPGIP
HHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCC
AEVLGQFASQLTQDLDKTVERFLAIQAMGSQTAKADIKQLRELVLARPSAQQPALAQGLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
MLENVDLRSQLSQIDQPWLRVWGRLDGLVPRRVIKAMPQVSASEDMLLTKASHAPFISHQ
HHHCCCHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHEEECCCCCHHHHH
AEFLDGYKLWLDKISN
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQIKLHVESIGQGPDVVMLHGWGVNSAVFTPLHSELNNYRFHYVDLPGFGHSQAVMGNI
CCEEEEHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
EHWLEAIMEQVPEKAIWTGWSLGGLVATLAAIKQPQRVSALCTIASSPCFMAREDWPGIP
HHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCC
AEVLGQFASQLTQDLDKTVERFLAIQAMGSQTAKADIKQLRELVLARPSAQQPALAQGLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
MLENVDLRSQLSQIDQPWLRVWGRLDGLVPRRVIKAMPQVSASEDMLLTKASHAPFISHQ
HHHCCCHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHEEECCCCCHHHHH
AEFLDGYKLWLDKISN
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA