Click here to switch to the map view.

The map label for this gene is dsbA [H]

Identifier: 167622176

GI number: 167622176

Start: 281764

End: 282375

Strand: Reverse

Name: dsbA [H]

Synonym: Shal_0235

Alternate gene names: 167622176

Gene position: 282375-281764 (Counterclockwise)

Preceding gene: 167622180

Following gene: 167622175

Centisome position: 5.4

GC content: 42.81

Gene sequence:

>612_bases
ATGAAAAAAACACTTACCGCCATCGCAGCAACTCTATTTCTATCATTTGGTGCCCATGCAGCAACAGAGGGAGTTGAGTA
CCAAGTATTACCGGAAGTGGCTCAATTTAACGCCCCAAACCAAGTAGTGAAGATCTACTCGATTAACTGTCCTTTCTGCT
ACAAGTATGAAAAAGCAGGTATTCCTAACGATAAGTTAATGCCTGCTGGCAGCACTTTGGATCAGTACCATATCACCAGT
AAACCACCTTTTGGCGTAGAGAAATCAACGGCATTGGCCATCGCTAAAGAGATCAAAGGCGAGAAGACATTCAAGCAATT
AAAGGACAAGTACTACGACCAGTACCACGTCAAGAAGGTCAAATTTAAAGATGCCGATTCTACCATTGCATTTACCCTCG
ATACATTAGGCATGAGCCGTGCCGAGTTTGATACTCATGCTCAAAATCCAAAAGTAAAACAGCTAATGACCAAATGGGAT
AAAGGGGTTGAAGTTGCAAAGATCCAAGGTGTGCCTGCGATTACCGTTAACGGCAAATACCTGATCAACACCAAATCAAT
CCGTTCGATGGATATGCTCAAAGAGCTTATCGCTGAACTGTCTGCTAAATAG

Upstream 100 bases:

>100_bases
AACAAACAATGACGTTTTATTTTTACATCGACAAAAACAATAACTATTAACAATATAAGTTTTCAAAATCCAACAAATTT
AATATATCTTTGGAGATAAC

Downstream 100 bases:

>100_bases
GGGATTATTATGTCATTGCAGCAACGATTGAAAGAGTTCTGGAGGCAGTTTAAGGCCACCCCTGCCGACAGCTTAACCCA
TATGCAAGACCATCGACCCA

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MKKTLTAIAATLFLSFGAHAATEGVEYQVLPEVAQFNAPNQVVKIYSINCPFCYKYEKAGIPNDKLMPAGSTLDQYHITS
KPPFGVEKSTALAIAKEIKGEKTFKQLKDKYYDQYHVKKVKFKDADSTIAFTLDTLGMSRAEFDTHAQNPKVKQLMTKWD
KGVEVAKIQGVPAITVNGKYLINTKSIRSMDMLKELIAELSAK

Sequences:

>Translated_203_residues
MKKTLTAIAATLFLSFGAHAATEGVEYQVLPEVAQFNAPNQVVKIYSINCPFCYKYEKAGIPNDKLMPAGSTLDQYHITS
KPPFGVEKSTALAIAKEIKGEKTFKQLKDKYYDQYHVKKVKFKDADSTIAFTLDTLGMSRAEFDTHAQNPKVKQLMTKWD
KGVEVAKIQGVPAITVNGKYLINTKSIRSMDMLKELIAELSAK
>Mature_203_residues
MKKTLTAIAATLFLSFGAHAATEGVEYQVLPEVAQFNAPNQVVKIYSINCPFCYKYEKAGIPNDKLMPAGSTLDQYHITS
KPPFGVEKSTALAIAKEIKGEKTFKQLKDKYYDQYHVKKVKFKDADSTIAFTLDTLGMSRAEFDTHAQNPKVKQLMTKWD
KGVEVAKIQGVPAITVNGKYLINTKSIRSMDMLKELIAELSAK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=198, Percent_Identity=29.2929292929293, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 22617; Mature: 22617

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLTAIAATLFLSFGAHAATEGVEYQVLPEVAQFNAPNQVVKIYSINCPFCYKYEKAG
CCHHHHHHHHHHHHHHCCHHHCCCCCEEECHHHHHCCCCCCEEEEEECCCCEEEEECCCC
IPNDKLMPAGSTLDQYHITSKPPFGVEKSTALAIAKEIKGEKTFKQLKDKYYDQYHVKKV
CCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHEEEE
KFKDADSTIAFTLDTLGMSRAEFDTHAQNPKVKQLMTKWDKGVEVAKIQGVPAITVNGKY
EECCCCCEEEEEEECCCCCHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEECCEE
LINTKSIRSMDMLKELIAELSAK
EEECHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKTLTAIAATLFLSFGAHAATEGVEYQVLPEVAQFNAPNQVVKIYSINCPFCYKYEKAG
CCHHHHHHHHHHHHHHCCHHHCCCCCEEECHHHHHCCCCCCEEEEEECCCCEEEEECCCC
IPNDKLMPAGSTLDQYHITSKPPFGVEKSTALAIAKEIKGEKTFKQLKDKYYDQYHVKKV
CCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHEEEE
KFKDADSTIAFTLDTLGMSRAEFDTHAQNPKVKQLMTKWDKGVEVAKIQGVPAITVNGKY
EECCCCCEEEEEEECCCCCHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEECCEE
LINTKSIRSMDMLKELIAELSAK
EEECHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10600454 [H]