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The map label for this gene is menF [H]

Identifier: 167622096

GI number: 167622096

Start: 180017

End: 181375

Strand: Reverse

Name: menF [H]

Synonym: Shal_0155

Alternate gene names: 167622096

Gene position: 181375-180017 (Counterclockwise)

Preceding gene: 167622105

Following gene: 167622095

Centisome position: 3.47

GC content: 46.8

Gene sequence:

>1359_bases
TTGACCATCCCCTTGCTGCCTCAAGCCGTTAAGTCTTTAACCGACAAAATCAAATCCTTTACTCTTCAACCAAACAGCGA
CCCCATTGTCCAGCTGTCCGTGGTGGTTTCGCCGATCCCAGTTATCGCTTGGCTAGCAGCACAATCAACTTACCCGAGAG
TGTATTGGAAGGGACGTGATACCGAAGAGGAAGTGGCCGCCATTGGCAGTTGTAAAGACTTTTTCTTTGGTGATGAAGTT
GATGATATCGAGCTGTCTGTCGAATATCAGAAACAAAGGGCACTTACCAATAATCAAGAGATCCGTTATTACGGCGGAGT
GGCTTTCGACCGTAGAAACGAAAGCTGGCCTGATTTTGGTAAAGCGCATTTTGTTCTGCCTCGCATAGAGCTGCGTCGCA
GTGGTAATGACTACAAACTTTTGGTTAATCTCAACTTTACCGAGCAAGAATTCACTCAAGAAATTGATGATGCGCTGCAA
TCATTACAAGCCTTAGACGCACCTAAGCCACTAGCACCACCAAATAAAGTGGCACTAATGGGCCGTAGCGATCGACCCGA
TCGGTATCGTTGGGACGAGCTAGTTAACCGTGTAACACATCCCAAGTTTATTGAAGACACCCCCAAAGTGGTCTTGTCGC
GACTGACTCAACTTGAGATTAACGAGCAGGTTGACCCTTGGATGCTATTGGCTTGCTGGCAAGGTCGTAACCCTAATAGT
TTTCAATTTGGTTTTCAGTTTAGCCCAGATAGTACCTTTATCTCTTGCACCCCAGAGCGCTTGTATCGCCGTCGTCAACG
TGAGCTATTTACCGAAGCACTCGCTGGCACTACGATTCGAGGGCTAAACCAAGAAGAAGATAAGGCGCTAGCGCAGGAGT
TACTGGATGACACCAAGAACAGTCATGAAAACCAGTTGGTTAGGCAGCATATTGTCGATGTACTTAACCCACTGAGTAAC
TATGTTGGCGCTGAAGAAACCCCAACAGTATTTAAACTAAGCCATATTCAGCATCTACACCGCTCTATTCGTGCCGAACT
AAAGCCCGGTGTGAATGACTTTCAGCTGATCCAAGCCCTGCACCCAACACCTGCCGTAGGCGGCCTTCCAAAAGAGTCGG
CTATGAATTTTATTCGCCAGCAAGAAGGTTATGTAAGAGGCTGGTATGCGGGAGCATGCGGTTATTTTAATAAGTATGAA
AGCGAATTTGCCGTGGCAATACGCAGCGCATTGATTGAGCCCGGTCGTATCAATCTATTTGCTGGTGCAGGCATTGTCGC
GGGCTCAGAAGCCGATAAAGAGTGGACCGAGTTAGAAAATAAACTCACCACCATTTTGTCTATCTTAACTGAATTGTAA

Upstream 100 bases:

>100_bases
GTTTGTGGTAAAGATTCTTATTACCAATGTGCGATGGGTGTATCCCGATTAGAGGCAGCTCCTCTAAGCAAGCCCTAATG
ATATTTGTTTGGAGTACTTA

Downstream 100 bases:

>100_bases
TCTCATATCAGGCTTCAGCTAATGTTAACTATACCAGAGTCGCACATTTGAGTGCAGTATAACTAGCCGACGAACACCGG
CTAGTATAACACCATGATAT

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTIPLLPQAVKSLTDKIKSFTLQPNSDPIVQLSVVVSPIPVIAWLAAQSTYPRVYWKGRDTEEEVAAIGSCKDFFFGDEV
DDIELSVEYQKQRALTNNQEIRYYGGVAFDRRNESWPDFGKAHFVLPRIELRRSGNDYKLLVNLNFTEQEFTQEIDDALQ
SLQALDAPKPLAPPNKVALMGRSDRPDRYRWDELVNRVTHPKFIEDTPKVVLSRLTQLEINEQVDPWMLLACWQGRNPNS
FQFGFQFSPDSTFISCTPERLYRRRQRELFTEALAGTTIRGLNQEEDKALAQELLDDTKNSHENQLVRQHIVDVLNPLSN
YVGAEETPTVFKLSHIQHLHRSIRAELKPGVNDFQLIQALHPTPAVGGLPKESAMNFIRQQEGYVRGWYAGACGYFNKYE
SEFAVAIRSALIEPGRINLFAGAGIVAGSEADKEWTELENKLTTILSILTEL

Sequences:

>Translated_452_residues
MTIPLLPQAVKSLTDKIKSFTLQPNSDPIVQLSVVVSPIPVIAWLAAQSTYPRVYWKGRDTEEEVAAIGSCKDFFFGDEV
DDIELSVEYQKQRALTNNQEIRYYGGVAFDRRNESWPDFGKAHFVLPRIELRRSGNDYKLLVNLNFTEQEFTQEIDDALQ
SLQALDAPKPLAPPNKVALMGRSDRPDRYRWDELVNRVTHPKFIEDTPKVVLSRLTQLEINEQVDPWMLLACWQGRNPNS
FQFGFQFSPDSTFISCTPERLYRRRQRELFTEALAGTTIRGLNQEEDKALAQELLDDTKNSHENQLVRQHIVDVLNPLSN
YVGAEETPTVFKLSHIQHLHRSIRAELKPGVNDFQLIQALHPTPAVGGLPKESAMNFIRQQEGYVRGWYAGACGYFNKYE
SEFAVAIRSALIEPGRINLFAGAGIVAGSEADKEWTELENKLTTILSILTEL
>Mature_451_residues
TIPLLPQAVKSLTDKIKSFTLQPNSDPIVQLSVVVSPIPVIAWLAAQSTYPRVYWKGRDTEEEVAAIGSCKDFFFGDEVD
DIELSVEYQKQRALTNNQEIRYYGGVAFDRRNESWPDFGKAHFVLPRIELRRSGNDYKLLVNLNFTEQEFTQEIDDALQS
LQALDAPKPLAPPNKVALMGRSDRPDRYRWDELVNRVTHPKFIEDTPKVVLSRLTQLEINEQVDPWMLLACWQGRNPNSF
QFGFQFSPDSTFISCTPERLYRRRQRELFTEALAGTTIRGLNQEEDKALAQELLDDTKNSHENQLVRQHIVDVLNPLSNY
VGAEETPTVFKLSHIQHLHRSIRAELKPGVNDFQLIQALHPTPAVGGLPKESAMNFIRQQEGYVRGWYAGACGYFNKYES
EFAVAIRSALIEPGRINLFAGAGIVAGSEADKEWTELENKLTTILSILTEL

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=427, Percent_Identity=33.7236533957845, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1786809, Length=269, Percent_Identity=28.996282527881, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787518, Length=223, Percent_Identity=30.4932735426009, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788114, Length=204, Percent_Identity=28.921568627451, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=324, Percent_Identity=28.3950617283951, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324361, Length=410, Percent_Identity=21.7073170731707, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 51412; Mature: 51281

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPLLPQAVKSLTDKIKSFTLQPNSDPIVQLSVVVSPIPVIAWLAAQSTYPRVYWKGRD
CCCCCCHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEECCCC
TEEEVAAIGSCKDFFFGDEVDDIELSVEYQKQRALTNNQEIRYYGGVAFDRRNESWPDFG
CHHHHHHHHCHHHHCCCCCCCCEEEEEEHHHHHHCCCCCCEEEECCEEECCCCCCCCCCC
KAHFVLPRIELRRSGNDYKLLVNLNFTEQEFTQEIDDALQSLQALDAPKPLAPPNKVALM
CCEEEECHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
GRSDRPDRYRWDELVNRVTHPKFIEDTPKVVLSRLTQLEINEQVDPWMLLACWQGRNPNS
CCCCCCCCCHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
FQFGFQFSPDSTFISCTPERLYRRRQRELFTEALAGTTIRGLNQEEDKALAQELLDDTKN
EEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
SHENQLVRQHIVDVLNPLSNYVGAEETPTVFKLSHIQHLHRSIRAELKPGVNDFQLIQAL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
HPTPAVGGLPKESAMNFIRQQEGYVRGWYAGACGYFNKYESEFAVAIRSALIEPGRINLF
CCCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AGAGIVAGSEADKEWTELENKLTTILSILTEL
ECCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIPLLPQAVKSLTDKIKSFTLQPNSDPIVQLSVVVSPIPVIAWLAAQSTYPRVYWKGRD
CCCCCHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEECCCC
TEEEVAAIGSCKDFFFGDEVDDIELSVEYQKQRALTNNQEIRYYGGVAFDRRNESWPDFG
CHHHHHHHHCHHHHCCCCCCCCEEEEEEHHHHHHCCCCCCEEEECCEEECCCCCCCCCCC
KAHFVLPRIELRRSGNDYKLLVNLNFTEQEFTQEIDDALQSLQALDAPKPLAPPNKVALM
CCEEEECHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
GRSDRPDRYRWDELVNRVTHPKFIEDTPKVVLSRLTQLEINEQVDPWMLLACWQGRNPNS
CCCCCCCCCHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
FQFGFQFSPDSTFISCTPERLYRRRQRELFTEALAGTTIRGLNQEEDKALAQELLDDTKN
EEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
SHENQLVRQHIVDVLNPLSNYVGAEETPTVFKLSHIQHLHRSIRAELKPGVNDFQLIQAL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
HPTPAVGGLPKESAMNFIRQQEGYVRGWYAGACGYFNKYESEFAVAIRSALIEPGRINLF
CCCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AGAGIVAGSEADKEWTELENKLTTILSILTEL
ECCCEEECCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]