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The map label for this gene is cusS [H]

Identifier: 167622074

GI number: 167622074

Start: 146891

End: 148228

Strand: Direct

Name: cusS [H]

Synonym: Shal_0133

Alternate gene names: 167622074

Gene position: 146891-148228 (Clockwise)

Preceding gene: 167622073

Following gene: 167622075

Centisome position: 2.81

GC content: 48.06

Gene sequence:

>1338_bases
GTGCTGGATATCGGTTACGCCAAGGGCATTAAGCCCTATCAGAGTAGTGCTTGGCGCATTACCATCATATTTTCAGCCCT
AGTAACCGTCATTATTGGCACCTTGTTACTGGGGATGTATCGTCAGCTGATCAATGAACAAGAGCATCAGTTGGACCTGC
ATCTCGATGCTGAAAGGACTCGCTATCAACAGATGGCACTGACCCTGGATAGACGTAGCTTTGCGACTCAGGTACGCGGT
GCCGATCCTAAAGTTGCCTTGATTGTCTGGCGCGACACCGCCGATCTTGTCGGCTCTCTAAGCATGATCCCTAAAGATAT
GCCTATGTTGCCCGAGAAGCATGAGTTTCCGGTATTTACTGGTGGTCCCGAAAAGCTACACATCTTAACTGGTGGCATGG
TGATGACGCGCTATGGTCCTATGCTTATCGCGACCCGAGTGGATCAGCTCGGTGCCTTAATCGATAAATTTACCAATGCC
GCAATTACTGCACTGATGTTGACCCTTGTGCTGACGCTGGCGCTGGGCTATCTATTTTCTAAAGCCATTTTACGCCGTCT
CGTACAGTACAACCGCTTGAGCAAGCGGATTGAACAGGGGCAATATTCGACTCGATTACCAGTGAGCTGGCGTCAAGATG
AATTTGATATGCTGGCACGGCAGTTTAACGGAGTACTCGATGCACTAGAGCAGAACTTAGCGGCTGTGCGTGGGGTCACC
GACAATATCGCCCATGATCTACGTACACCTTTGTCTCACTTGCGTATTGGGCTTGAGCAACTGCCGAGTAAACCCAAAGA
GGAGCTTGAAGAGGGCTGTGCCATCCTCACCGAAGAGCTAGATCATTGCTTAGCGACTTTCGATGCCATGCTGTCGTTGA
CGCGCATCGAAGAGGGTCAGCAAACTCTGGAGTTACAAGATGTCAGCTTAATCAATATCAGCCAAGATCTGTTTGAAATG
GCGGATGCGATGGCCGAATTTAATGGTCAATCATTGAAACTGACAACGGGTGAAGAGTTTCAGGTCCGTGGTGACAAGTA
TCTATTGTTCCAAGCGCTATTTAACTTGGTCGATAATGCCGTTAAATATTCTGGAGAAGGTGCTGAAATTTGCATTTCAC
AGAATCGCAATGTCATCTCTATTCAAGATAATGGTCCGGGGATCCCTGAGGCTTCTCGTGAGAAGGTATTCGAGCGCTTG
GTGCGTTTAGACCCCAGTCGTCACCACAAAGGCACAGGGTTAGGTTTATCTATGGTGAAGGCGATTCTGTCGCGACATAA
TGCTAAAATAGCGCTAACCGATAATCAGCCTGGCCTAAAGGTTATTATTACTTTTTAA

Upstream 100 bases:

>100_bases
CATTTCGATCCGCGTACTAATGTTATCGATGTGCATATCGCTAAGCTAAGACGTAAGTTTGAAGAGCTAGGCCATGGCGA
ATTAATCGAAACGGTTCGAG

Downstream 100 bases:

>100_bases
TCTTTGTGCTAGCCAAGCTACGCTACTGCCAACCTGTGGAACCCCCTATCTATGTATCGAGTCATTGCCGATGAGCACGA
CTTTATTGTTATTAATAAGT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MLDIGYAKGIKPYQSSAWRITIIFSALVTVIIGTLLLGMYRQLINEQEHQLDLHLDAERTRYQQMALTLDRRSFATQVRG
ADPKVALIVWRDTADLVGSLSMIPKDMPMLPEKHEFPVFTGGPEKLHILTGGMVMTRYGPMLIATRVDQLGALIDKFTNA
AITALMLTLVLTLALGYLFSKAILRRLVQYNRLSKRIEQGQYSTRLPVSWRQDEFDMLARQFNGVLDALEQNLAAVRGVT
DNIAHDLRTPLSHLRIGLEQLPSKPKEELEEGCAILTEELDHCLATFDAMLSLTRIEEGQQTLELQDVSLINISQDLFEM
ADAMAEFNGQSLKLTTGEEFQVRGDKYLLFQALFNLVDNAVKYSGEGAEICISQNRNVISIQDNGPGIPEASREKVFERL
VRLDPSRHHKGTGLGLSMVKAILSRHNAKIALTDNQPGLKVIITF

Sequences:

>Translated_445_residues
MLDIGYAKGIKPYQSSAWRITIIFSALVTVIIGTLLLGMYRQLINEQEHQLDLHLDAERTRYQQMALTLDRRSFATQVRG
ADPKVALIVWRDTADLVGSLSMIPKDMPMLPEKHEFPVFTGGPEKLHILTGGMVMTRYGPMLIATRVDQLGALIDKFTNA
AITALMLTLVLTLALGYLFSKAILRRLVQYNRLSKRIEQGQYSTRLPVSWRQDEFDMLARQFNGVLDALEQNLAAVRGVT
DNIAHDLRTPLSHLRIGLEQLPSKPKEELEEGCAILTEELDHCLATFDAMLSLTRIEEGQQTLELQDVSLINISQDLFEM
ADAMAEFNGQSLKLTTGEEFQVRGDKYLLFQALFNLVDNAVKYSGEGAEICISQNRNVISIQDNGPGIPEASREKVFERL
VRLDPSRHHKGTGLGLSMVKAILSRHNAKIALTDNQPGLKVIITF
>Mature_445_residues
MLDIGYAKGIKPYQSSAWRITIIFSALVTVIIGTLLLGMYRQLINEQEHQLDLHLDAERTRYQQMALTLDRRSFATQVRG
ADPKVALIVWRDTADLVGSLSMIPKDMPMLPEKHEFPVFTGGPEKLHILTGGMVMTRYGPMLIATRVDQLGALIDKFTNA
AITALMLTLVLTLALGYLFSKAILRRLVQYNRLSKRIEQGQYSTRLPVSWRQDEFDMLARQFNGVLDALEQNLAAVRGVT
DNIAHDLRTPLSHLRIGLEQLPSKPKEELEEGCAILTEELDHCLATFDAMLSLTRIEEGQQTLELQDVSLINISQDLFEM
ADAMAEFNGQSLKLTTGEEFQVRGDKYLLFQALFNLVDNAVKYSGEGAEICISQNRNVISIQDNGPGIPEASREKVFERL
VRLDPSRHHKGTGLGLSMVKAILSRHNAKIALTDNQPGLKVIITF

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=332, Percent_Identity=28.0120481927711, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788393, Length=260, Percent_Identity=30.7692307692308, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787894, Length=266, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788279, Length=280, Percent_Identity=26.7857142857143, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790551, Length=284, Percent_Identity=27.4647887323944, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790346, Length=237, Percent_Identity=27.4261603375527, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786600, Length=207, Percent_Identity=28.0193236714976, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789808, Length=281, Percent_Identity=25.6227758007117, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789403, Length=213, Percent_Identity=27.6995305164319, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787374, Length=282, Percent_Identity=25.177304964539, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786912, Length=204, Percent_Identity=28.4313725490196, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788713, Length=214, Percent_Identity=27.1028037383178, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=204, Percent_Identity=26.9607843137255, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790300, Length=220, Percent_Identity=28.1818181818182, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789149, Length=211, Percent_Identity=26.5402843601896, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49860; Mature: 49860

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIGYAKGIKPYQSSAWRITIIFSALVTVIIGTLLLGMYRQLINEQEHQLDLHLDAERT
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEECHHHH
RYQQMALTLDRRSFATQVRGADPKVALIVWRDTADLVGSLSMIPKDMPMLPEKHEFPVFT
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEE
GGPEKLHILTGGMVMTRYGPMLIATRVDQLGALIDKFTNAAITALMLTLVLTLALGYLFS
CCCCEEEEEECCEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAILRRLVQYNRLSKRIEQGQYSTRLPVSWRQDEFDMLARQFNGVLDALEQNLAAVRGVT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
DNIAHDLRTPLSHLRIGLEQLPSKPKEELEEGCAILTEELDHCLATFDAMLSLTRIEEGQ
HHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
QTLELQDVSLINISQDLFEMADAMAEFNGQSLKLTTGEEFQVRGDKYLLFQALFNLVDNA
HHHHHCCCEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHH
VKYSGEGAEICISQNRNVISIQDNGPGIPEASREKVFERLVRLDPSRHHKGTGLGLSMVK
HHCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
AILSRHNAKIALTDNQPGLKVIITF
HHHHCCCCEEEEECCCCCCEEEEEC
>Mature Secondary Structure
MLDIGYAKGIKPYQSSAWRITIIFSALVTVIIGTLLLGMYRQLINEQEHQLDLHLDAERT
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEECHHHH
RYQQMALTLDRRSFATQVRGADPKVALIVWRDTADLVGSLSMIPKDMPMLPEKHEFPVFT
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEE
GGPEKLHILTGGMVMTRYGPMLIATRVDQLGALIDKFTNAAITALMLTLVLTLALGYLFS
CCCCEEEEEECCEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAILRRLVQYNRLSKRIEQGQYSTRLPVSWRQDEFDMLARQFNGVLDALEQNLAAVRGVT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
DNIAHDLRTPLSHLRIGLEQLPSKPKEELEEGCAILTEELDHCLATFDAMLSLTRIEEGQ
HHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
QTLELQDVSLINISQDLFEMADAMAEFNGQSLKLTTGEEFQVRGDKYLLFQALFNLVDNA
HHHHHCCCEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHH
VKYSGEGAEICISQNRNVISIQDNGPGIPEASREKVFERLVRLDPSRHHKGTGLGLSMVK
HHCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
AILSRHNAKIALTDNQPGLKVIITF
HHHHCCCCEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]