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The map label for this gene is glyS

Identifier: 167621947

GI number: 167621947

Start: 7531

End: 9600

Strand: Reverse

Name: glyS

Synonym: Shal_0006

Alternate gene names: 167621947

Gene position: 9600-7531 (Counterclockwise)

Preceding gene: 167621948

Following gene: NA

Centisome position: 0.18

GC content: 47.44

Gene sequence:

>2070_bases
ATGAATTTTGAAAACTTACTGATTGAAGTAGGCACTGAAGAGTTACCACCAAAGTCACTGCGTAAACTGGCCGAATCTTT
TCTTGCCAACTTTACCGAAGAGCTAACCAAAGCAGAGCTCAGCTTCGACTCTGCCGTATGGCACGCGTCTCCACGCCGTT
TAGCGATTTGTATTAACCAGCTAGCACTTGCGCAAGCAGATAAAGTGGTTGAAAAGCGCGGACCTGCTATAGCGCAAGCT
TTTGATGCCGATGGCAACCCAACTAAAGCTGCTCAAGGCTGGGCTCGTGGTAACGGGATTTCCGTCGAGCAAGCTGAACG
CTTAAAGACTGACAAAGGCGAATGGTTACTACACCAAGCACGCGTTGTCGGTGTTGAAACTAAGAGCCTGATTGCCGATA
TGGCGCAGCGCTCTTTAGATAAGTTACCTATCCCTAAGCCAATGCGCTGGGGTAGCAACACGACTCAATTTATCCGCCCG
GTTCACACTGTCACTATGCTACTGGGTAGCGAAGTGGTTGAAGGCGAGCTACTAGGCATTAAATCTGATCGCGTTATTCG
TGGCCACCGCTTTATGGGTGAGTCTAGCTTCGAGCTAGATCACGCAGATAACTACCTTGTAGCACTAAAAGAAAAAGGTA
AAGTATTAGCGGATTACCAAGCTCGTAAAGCCATCATTAAAACTGATGCTGAAGCGGCTGCAGCTAAAATTGGCGGCGTA
GCCGATCTTGAAGATGACCTGCTTGAAGAAGTGACTTCTCTAGTTGAATGGCCAGTAGTACTGACAGCCAGCTTCGAAGA
GAAATTCTTAGATGTACCAGCAGAAGCTTTGGTTTACACCATGAAAGGTGACCAAAAGTACTTCCCTGTATTTGATAATG
CCGGCCAGTTACTACCTAACTTTATCTTCGTGACTAACATCGAGTCGAAAGATCCACAGCAGATTATTTCTGGTAACGAG
AAAGTGGTTCGCCCACGCCTTGCCGATGCTGAGTTCTTCTTCGAAACAGACAAGAAAGAATCTCTTGAGGCACGTCTTGC
CAGCTTAGAGACCGTGGTATTCCAAAAGCAACTTGGCACCATCAAGCAGCGTGTTGAACGTATCTCTGCTTTAGCGGGTT
ATATCGCCACCAGCATCAACGCTAATAGCGAAGAAGCTGCGCGCGCGGGTCTGCTATCTAAGTCTGACTTGATGACTAAT
ATGGTCATGGAGTTTACCGATCTTCAAGGCACCATGGGTATGCATTACGCACGCCTAAATGGTGAAACTGAAGCGGTTGC
TGTAGCCTTAGCGGAGCAATACAAGCCTAAGTTCTCAGGCGATACCGTGCCAACTGCACCTATCTCTATCTGTGTAGCAC
TAGCTGAAAAGCTCGACACGTTAGTGGGTATTTTTGGTATTGGCCAAGCGCCTAAAGGTGCTGCGGATCCATTTGCACTA
CGCCGCGCAGCAATTGGTGTACTACGTATCTGTTTAGAGAACAACTTACCACTAGATCTTGTTGATTTAATTGCTAAAGC
ACAAGAGCTACACGGTGAGAATCTCACTAACGAAAACGTTGCTGAGCAAGTACTTGAGTTCTTCATGGGCCGTTTCCGTG
CCTGGTACCAAGATCAAGGTGTGAGTGTAGACGTTATTCTAGCAGTACTCGCTCGTCGTCCAACGGCTCCAGCAGACTTT
GAAAGCCGCATTAAAGCCGTTGCTCACTTCAGAACGCTTGAACAAGCCTCAGCACTAGCTGCAGCTAATAAGCGTGTATC
AAACATCTTGGCTAAAGTTGAAGGTGAGCTTCCAGCTGCAATCGACGATAAGCTACTCGTTGAAGAAGCTGAAAAGGCGC
TTGCTGCTAAACTTGCTGAGCTTCAGCCACAGCTAGCACCACTATTTGCAGCAGCTAACTATCAAGAAGCCCTAGCACTG
CTTGCTAGCCTTCGTGAAAGCGTTGATACCTTCTTCGAAGATGTGATGGTTATGGCAGATGATGAAGCACTTAAGAACAA
CCGCCTAGCGCTACTTTCTAGCCTACGTGAGCAGTTCCTACATGCGGCAGATATCTCGCTACTGCAATAA

Upstream 100 bases:

>100_bases
ATATCTTACGCGTTCGTACCATGGCTAAAGGCGTTGCCGAGGCTTACTATCAAGCCCGTGAAGCTCTTGGCTTCCCAATG
TGTAAGTAGAGGAATAACCA

Downstream 100 bases:

>100_bases
TATTTGTAGTAAACCAGATATAAAAAACGCGCCGATTGGCGCGTTTTTGTTTGAGTGACTTTACAGCCTAAAACAGTATG
AGCTTCTGATGCAGTTGTTC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MNFENLLIEVGTEELPPKSLRKLAESFLANFTEELTKAELSFDSAVWHASPRRLAICINQLALAQADKVVEKRGPAIAQA
FDADGNPTKAAQGWARGNGISVEQAERLKTDKGEWLLHQARVVGVETKSLIADMAQRSLDKLPIPKPMRWGSNTTQFIRP
VHTVTMLLGSEVVEGELLGIKSDRVIRGHRFMGESSFELDHADNYLVALKEKGKVLADYQARKAIIKTDAEAAAAKIGGV
ADLEDDLLEEVTSLVEWPVVLTASFEEKFLDVPAEALVYTMKGDQKYFPVFDNAGQLLPNFIFVTNIESKDPQQIISGNE
KVVRPRLADAEFFFETDKKESLEARLASLETVVFQKQLGTIKQRVERISALAGYIATSINANSEEAARAGLLSKSDLMTN
MVMEFTDLQGTMGMHYARLNGETEAVAVALAEQYKPKFSGDTVPTAPISICVALAEKLDTLVGIFGIGQAPKGAADPFAL
RRAAIGVLRICLENNLPLDLVDLIAKAQELHGENLTNENVAEQVLEFFMGRFRAWYQDQGVSVDVILAVLARRPTAPADF
ESRIKAVAHFRTLEQASALAAANKRVSNILAKVEGELPAAIDDKLLVEEAEKALAAKLAELQPQLAPLFAAANYQEALAL
LASLRESVDTFFEDVMVMADDEALKNNRLALLSSLREQFLHAADISLLQ

Sequences:

>Translated_689_residues
MNFENLLIEVGTEELPPKSLRKLAESFLANFTEELTKAELSFDSAVWHASPRRLAICINQLALAQADKVVEKRGPAIAQA
FDADGNPTKAAQGWARGNGISVEQAERLKTDKGEWLLHQARVVGVETKSLIADMAQRSLDKLPIPKPMRWGSNTTQFIRP
VHTVTMLLGSEVVEGELLGIKSDRVIRGHRFMGESSFELDHADNYLVALKEKGKVLADYQARKAIIKTDAEAAAAKIGGV
ADLEDDLLEEVTSLVEWPVVLTASFEEKFLDVPAEALVYTMKGDQKYFPVFDNAGQLLPNFIFVTNIESKDPQQIISGNE
KVVRPRLADAEFFFETDKKESLEARLASLETVVFQKQLGTIKQRVERISALAGYIATSINANSEEAARAGLLSKSDLMTN
MVMEFTDLQGTMGMHYARLNGETEAVAVALAEQYKPKFSGDTVPTAPISICVALAEKLDTLVGIFGIGQAPKGAADPFAL
RRAAIGVLRICLENNLPLDLVDLIAKAQELHGENLTNENVAEQVLEFFMGRFRAWYQDQGVSVDVILAVLARRPTAPADF
ESRIKAVAHFRTLEQASALAAANKRVSNILAKVEGELPAAIDDKLLVEEAEKALAAKLAELQPQLAPLFAAANYQEALAL
LASLRESVDTFFEDVMVMADDEALKNNRLALLSSLREQFLHAADISLLQ
>Mature_689_residues
MNFENLLIEVGTEELPPKSLRKLAESFLANFTEELTKAELSFDSAVWHASPRRLAICINQLALAQADKVVEKRGPAIAQA
FDADGNPTKAAQGWARGNGISVEQAERLKTDKGEWLLHQARVVGVETKSLIADMAQRSLDKLPIPKPMRWGSNTTQFIRP
VHTVTMLLGSEVVEGELLGIKSDRVIRGHRFMGESSFELDHADNYLVALKEKGKVLADYQARKAIIKTDAEAAAAKIGGV
ADLEDDLLEEVTSLVEWPVVLTASFEEKFLDVPAEALVYTMKGDQKYFPVFDNAGQLLPNFIFVTNIESKDPQQIISGNE
KVVRPRLADAEFFFETDKKESLEARLASLETVVFQKQLGTIKQRVERISALAGYIATSINANSEEAARAGLLSKSDLMTN
MVMEFTDLQGTMGMHYARLNGETEAVAVALAEQYKPKFSGDTVPTAPISICVALAEKLDTLVGIFGIGQAPKGAADPFAL
RRAAIGVLRICLENNLPLDLVDLIAKAQELHGENLTNENVAEQVLEFFMGRFRAWYQDQGVSVDVILAVLARRPTAPADF
ESRIKAVAHFRTLEQASALAAANKRVSNILAKVEGELPAAIDDKLLVEEAEKALAAKLAELQPQLAPLFAAANYQEALAL
LASLRESVDTFFEDVMVMADDEALKNNRLALLSSLREQFLHAADISLLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=64.7314949201742, Blast_Score=896, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_SHEHH (B0TLA9)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672241.1
- ProteinModelPortal:   B0TLA9
- GeneID:   5906173
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0006
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- BioCyc:   SHAL458817:SHAL_0006-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75525; Mature: 75525

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFENLLIEVGTEELPPKSLRKLAESFLANFTEELTKAELSFDSAVWHASPRRLAICINQ
CCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LALAQADKVVEKRGPAIAQAFDADGNPTKAAQGWARGNGISVEQAERLKTDKGEWLLHQA
HHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCHHHHHHH
RVVGVETKSLIADMAQRSLDKLPIPKPMRWGSNTTQFIRPVHTVTMLLGSEVVEGELLGI
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHCCEEEEC
KSDRVIRGHRFMGESSFELDHADNYLVALKEKGKVLADYQARKAIIKTDAEAAAAKIGGV
CCCCEEECHHCCCCCCCCCCCCCCEEEEEECCCCEEECHHHHHHHHHCCHHHHHHHHCCC
ADLEDDLLEEVTSLVEWPVVLTASFEEKFLDVPAEALVYTMKGDQKYFPVFDNAGQLLPN
CCCHHHHHHHHHHHHHCCEEEEECHHHHHHCCCHHHEEEEECCCCEECCCCCCCHHHCCC
FIFVTNIESKDPQQIISGNEKVVRPRLADAEFFFETDKKESLEARLASLETVVFQKQLGT
EEEEEECCCCCHHHHHCCCCCEECCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKQRVERISALAGYIATSINANSEEAARAGLLSKSDLMTNMVMEFTDLQGTMGMHYARLN
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHCCEEEECC
GETEAVAVALAEQYKPKFSGDTVPTAPISICVALAEKLDTLVGIFGIGQAPKGAADPFAL
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAAIGVLRICLENNLPLDLVDLIAKAQELHGENLTNENVAEQVLEFFMGRFRAWYQDQG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VSVDVILAVLARRPTAPADFESRIKAVAHFRTLEQASALAAANKRVSNILAKVEGELPAA
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IDDKLLVEEAEKALAAKLAELQPQLAPLFAAANYQEALALLASLRESVDTFFEDVMVMAD
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DEALKNNRLALLSSLREQFLHAADISLLQ
CHHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFENLLIEVGTEELPPKSLRKLAESFLANFTEELTKAELSFDSAVWHASPRRLAICINQ
CCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LALAQADKVVEKRGPAIAQAFDADGNPTKAAQGWARGNGISVEQAERLKTDKGEWLLHQA
HHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCHHHHHHH
RVVGVETKSLIADMAQRSLDKLPIPKPMRWGSNTTQFIRPVHTVTMLLGSEVVEGELLGI
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHCCEEEEC
KSDRVIRGHRFMGESSFELDHADNYLVALKEKGKVLADYQARKAIIKTDAEAAAAKIGGV
CCCCEEECHHCCCCCCCCCCCCCCEEEEEECCCCEEECHHHHHHHHHCCHHHHHHHHCCC
ADLEDDLLEEVTSLVEWPVVLTASFEEKFLDVPAEALVYTMKGDQKYFPVFDNAGQLLPN
CCCHHHHHHHHHHHHHCCEEEEECHHHHHHCCCHHHEEEEECCCCEECCCCCCCHHHCCC
FIFVTNIESKDPQQIISGNEKVVRPRLADAEFFFETDKKESLEARLASLETVVFQKQLGT
EEEEEECCCCCHHHHHCCCCCEECCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKQRVERISALAGYIATSINANSEEAARAGLLSKSDLMTNMVMEFTDLQGTMGMHYARLN
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHCCEEEECC
GETEAVAVALAEQYKPKFSGDTVPTAPISICVALAEKLDTLVGIFGIGQAPKGAADPFAL
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAAIGVLRICLENNLPLDLVDLIAKAQELHGENLTNENVAEQVLEFFMGRFRAWYQDQG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VSVDVILAVLARRPTAPADFESRIKAVAHFRTLEQASALAAANKRVSNILAKVEGELPAA
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IDDKLLVEEAEKALAAKLAELQPQLAPLFAAANYQEALALLASLRESVDTFFEDVMVMAD
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DEALKNNRLALLSSLREQFLHAADISLLQ
CHHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA