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The map label for this gene is thiI [H]

Identifier: 167040647

GI number: 167040647

Start: 2033480

End: 2034622

Strand: Reverse

Name: thiI [H]

Synonym: Teth514_2024

Alternate gene names: 167040647

Gene position: 2034622-2033480 (Counterclockwise)

Preceding gene: 167040648

Following gene: 167040646

Centisome position: 82.8

GC content: 33.25

Gene sequence:

>1143_bases
ATGCAAGACATATTATTGATTAAATATGGAGAATTAGCTTTAAAAGGAGATAATAGGTCTTTTTTTGAAAATAAATTGAT
AAAAAATATAAAACATGCTCTTTCTGACTTTAAGGAAGTTAAAGTTGAAAAAACTCATGGCAGAATTTATGTAGAATGTG
ATGGAGATATTGAAGAGGTAATAGAAAGATTAAAAAAAGTCTTTGGTATTGTAGGAATAACAAAAGCTAAAAAAACCGAT
TTAAACTTGGATGAAATATTTAAAGCTGCAGTAGAACTTATGAAAGGACACGAAGGAAAGACTTTTAAAGTAGAGACTAA
GAGGCCAAATAAGTCTTTTCCTTATAACAGCATGGAGGTCAGCCGCAGAGTAGGAGCAGCAGTATTGAAAAATGTCAAAA
ACTTAAAAGTAGATGTTCATAATCCTGATGTGCTTTTAAATGTAGAGATAAGAGAAATGGCTTTTGTATACGCGGGAGTG
ATTGAGGGAATAGGAGGACTTCCTCTTGGGACAAACGGTAAAGCGACTGTACTTTTGTCAGGAGGAATTGACAGTCCTGT
AGCTGCTTGGATGATGATGAAAAGAGGCGTAGAAGTAGAAGCAGTTTATTTTCACAGCCCTCCTTATACTTCTGAAAGGG
CTAAAGACAAAGTTGTAGATTTGTGCAAAGTCCTTTCTCAATATGGACAAAGGATAAAATTACACGTAGTTCACTTTACT
GATTTGCAATTAGAAATTTATGAGAAATGTCCACCTAAATTTACTACTATAATTATGAGAAGAATGATGATGAAGATAGC
AGAAAAAATTGCTCAAAAAAATGGTTCTATGGCTCTAATCACAGGGGAAAGTTTAGGACAAGTTGCAAGCCAAACGATTG
AAAGTTTATATGTAACCAATGCTTCTGTCTCTATGCCAATATTTAGACCTCTTATTGGGATGGATAAGACAGAGATTATA
GATTTAGCTCAAAAGATTAGTACGTTTGAGATCTCTATAAGACCCTATGAAGATTGTTGCACTATCTTTGTGCCAAAACA
TCCTGCTACAAAGCCAAAATTAGAAAAAGTAATAGAAGCAGAACAAAAAATGGAGTATCAAAAATACATTGATAATTTTG
AAGAAGAGGTTATAGAAGTTTAA

Upstream 100 bases:

>100_bases
TTCCATTGGAATTTTTAATACAGAAGAAGAAATTGACTATACTATATCAGTGTTAAAAGAAAAAGTGAATTTTTTAAGAA
AGTATAAACGGAGGTAAAAT

Downstream 100 bases:

>100_bases
GCCAAGCAGCTTTGCTTGGTTTATTTTTATCCTTTTAAAAAATTAATTTTTTTAAACTAAAACTTAAAATAATTTAAAAT
TTATATTGACATTTTTAAAA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MQDILLIKYGELALKGDNRSFFENKLIKNIKHALSDFKEVKVEKTHGRIYVECDGDIEEVIERLKKVFGIVGITKAKKTD
LNLDEIFKAAVELMKGHEGKTFKVETKRPNKSFPYNSMEVSRRVGAAVLKNVKNLKVDVHNPDVLLNVEIREMAFVYAGV
IEGIGGLPLGTNGKATVLLSGGIDSPVAAWMMMKRGVEVEAVYFHSPPYTSERAKDKVVDLCKVLSQYGQRIKLHVVHFT
DLQLEIYEKCPPKFTTIIMRRMMMKIAEKIAQKNGSMALITGESLGQVASQTIESLYVTNASVSMPIFRPLIGMDKTEII
DLAQKISTFEISIRPYEDCCTIFVPKHPATKPKLEKVIEAEQKMEYQKYIDNFEEEVIEV

Sequences:

>Translated_380_residues
MQDILLIKYGELALKGDNRSFFENKLIKNIKHALSDFKEVKVEKTHGRIYVECDGDIEEVIERLKKVFGIVGITKAKKTD
LNLDEIFKAAVELMKGHEGKTFKVETKRPNKSFPYNSMEVSRRVGAAVLKNVKNLKVDVHNPDVLLNVEIREMAFVYAGV
IEGIGGLPLGTNGKATVLLSGGIDSPVAAWMMMKRGVEVEAVYFHSPPYTSERAKDKVVDLCKVLSQYGQRIKLHVVHFT
DLQLEIYEKCPPKFTTIIMRRMMMKIAEKIAQKNGSMALITGESLGQVASQTIESLYVTNASVSMPIFRPLIGMDKTEII
DLAQKISTFEISIRPYEDCCTIFVPKHPATKPKLEKVIEAEQKMEYQKYIDNFEEEVIEV
>Mature_380_residues
MQDILLIKYGELALKGDNRSFFENKLIKNIKHALSDFKEVKVEKTHGRIYVECDGDIEEVIERLKKVFGIVGITKAKKTD
LNLDEIFKAAVELMKGHEGKTFKVETKRPNKSFPYNSMEVSRRVGAAVLKNVKNLKVDVHNPDVLLNVEIREMAFVYAGV
IEGIGGLPLGTNGKATVLLSGGIDSPVAAWMMMKRGVEVEAVYFHSPPYTSERAKDKVVDLCKVLSQYGQRIKLHVVHFT
DLQLEIYEKCPPKFTTIIMRRMMMKIAEKIAQKNGSMALITGESLGQVASQTIESLYVTNASVSMPIFRPLIGMDKTEII
DLAQKISTFEISIRPYEDCCTIFVPKHPATKPKLEKVIEAEQKMEYQKYIDNFEEEVIEV

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=312, Percent_Identity=32.6923076923077, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 42953; Mature: 42953

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDILLIKYGELALKGDNRSFFENKLIKNIKHALSDFKEVKVEKTHGRIYVECDGDIEEV
CCCEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCEEEEEECCCHHHH
IERLKKVFGIVGITKAKKTDLNLDEIFKAAVELMKGHEGKTFKVETKRPNKSFPYNSMEV
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHH
SRRVGAAVLKNVKNLKVDVHNPDVLLNVEIREMAFVYAGVIEGIGGLPLGTNGKATVLLS
HHHHHHHHHHCHHHCEEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
GGIDSPVAAWMMMKRGVEVEAVYFHSPPYTSERAKDKVVDLCKVLSQYGQRIKLHVVHFT
CCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEE
DLQLEIYEKCPPKFTTIIMRRMMMKIAEKIAQKNGSMALITGESLGQVASQTIESLYVTN
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCC
ASVSMPIFRPLIGMDKTEIIDLAQKISTFEISIRPYEDCCTIFVPKHPATKPKLEKVIEA
CCCCCHHHHHHHCCCHHHHHHHHHHCCEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
EQKMEYQKYIDNFEEEVIEV
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDILLIKYGELALKGDNRSFFENKLIKNIKHALSDFKEVKVEKTHGRIYVECDGDIEEV
CCCEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCEEEEEECCCHHHH
IERLKKVFGIVGITKAKKTDLNLDEIFKAAVELMKGHEGKTFKVETKRPNKSFPYNSMEV
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHH
SRRVGAAVLKNVKNLKVDVHNPDVLLNVEIREMAFVYAGVIEGIGGLPLGTNGKATVLLS
HHHHHHHHHHCHHHCEEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
GGIDSPVAAWMMMKRGVEVEAVYFHSPPYTSERAKDKVVDLCKVLSQYGQRIKLHVVHFT
CCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEE
DLQLEIYEKCPPKFTTIIMRRMMMKIAEKIAQKNGSMALITGESLGQVASQTIESLYVTN
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCC
ASVSMPIFRPLIGMDKTEIIDLAQKISTFEISIRPYEDCCTIFVPKHPATKPKLEKVIEA
CCCCCHHHHHHHCCCHHHHHHHHHHCCEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
EQKMEYQKYIDNFEEEVIEV
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA