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The map label for this gene is spo0A [H]

Identifier: 167040184

GI number: 167040184

Start: 1569746

End: 1570528

Strand: Direct

Name: spo0A [H]

Synonym: Teth514_1546

Alternate gene names: 167040184

Gene position: 1569746-1570528 (Clockwise)

Preceding gene: 167040183

Following gene: 167040185

Centisome position: 63.88

GC content: 32.44

Gene sequence:

>783_bases
TTGGTTAAGAAGATTAGAGTAGGAATAGCAGATGATAACAAAGAGTTTGCTACTATTTTAGCTGAGTATTTATCTACTCA
AGAAAATATTGAAGTGGTAGGTGTTGCCAATGATGGCAACCAAGCGATTGAACTCATTAAAAATCAATCGCCAGATTTGT
TAATTTTAGATATTATAATGCCCTATTTAGATGGCATTGGTGTTTTGGAAAAGATAAATGAAGAACAATTAAAAAAGCCT
AAAATTTTAGTTTTATCTGCAGTAGGCCAAGAAAAAATAACTCAACGGGCTATTGCATTAGGAGCAGATTATTATATTTT
AAAACCTTTTGATTTAGCTATGTTGTCAAAAAGGATTTCTGAGCTTATGGAACCAGAATTAAATGTAGTATCAAAGACTG
TTTTTCCTGTGGTAAAAGCGAAGAAAAATTATGACCTTGAAGCAATGATTACAGAAATTATTCATGAGGTAGGAGTACCA
GCTCATATAAAAGGCTATATGTATCTAAGAGATGCTATTTCTCTTGTTATTAGCAATATGGAATATTTAAATTCTGTAAC
TAAAATGTTATATCCTAAAATTGCTGAAAAGTACAATACTACTCCTAGCAGAGTAGAAAGAGCTATAAGGCATGCAATAG
AAGTTGCGTGGAGCAGGGGCAAAACAGAGGTTTTAAATGAACTTTTTGGATATACTATAAATGACGAAAAAGGTAAACCG
ACAAATTCTGAATTCATTGCGCTTATTGCTGATAAATTGAGGTTGGGGATGAAAGCAGGGTAA

Upstream 100 bases:

>100_bases
TTTACAAATAACTAAAAATTTTTAAGGGAAAAAGAAGGAAATTCAAATATTATGTAGAATAAATATTATATACTAATATT
AGCAATGGGAGGTTAAATAA

Downstream 100 bases:

>100_bases
AAGGGGCAGATACCTGCTTTCTCTTTTTTAACCTCCCATTTAAAAATATGATAAACATAAAAGTAGAGCCCAAAATCCTT
CTATTTGTTGAGGATTCAAC

Product: response regulator receiver protein

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MVKKIRVGIADDNKEFATILAEYLSTQENIEVVGVANDGNQAIELIKNQSPDLLILDIIMPYLDGIGVLEKINEEQLKKP
KILVLSAVGQEKITQRAIALGADYYILKPFDLAMLSKRISELMEPELNVVSKTVFPVVKAKKNYDLEAMITEIIHEVGVP
AHIKGYMYLRDAISLVISNMEYLNSVTKMLYPKIAEKYNTTPSRVERAIRHAIEVAWSRGKTEVLNELFGYTINDEKGKP
TNSEFIALIADKLRLGMKAG

Sequences:

>Translated_260_residues
MVKKIRVGIADDNKEFATILAEYLSTQENIEVVGVANDGNQAIELIKNQSPDLLILDIIMPYLDGIGVLEKINEEQLKKP
KILVLSAVGQEKITQRAIALGADYYILKPFDLAMLSKRISELMEPELNVVSKTVFPVVKAKKNYDLEAMITEIIHEVGVP
AHIKGYMYLRDAISLVISNMEYLNSVTKMLYPKIAEKYNTTPSRVERAIRHAIEVAWSRGKTEVLNELFGYTINDEKGKP
TNSEFIALIADKLRLGMKAG
>Mature_260_residues
MVKKIRVGIADDNKEFATILAEYLSTQENIEVVGVANDGNQAIELIKNQSPDLLILDIIMPYLDGIGVLEKINEEQLKKP
KILVLSAVGQEKITQRAIALGADYYILKPFDLAMLSKRISELMEPELNVVSKTVFPVVKAKKNYDLEAMITEIIHEVGVP
AHIKGYMYLRDAISLVISNMEYLNSVTKMLYPKIAEKYNTTPSRVERAIRHAIEVAWSRGKTEVLNELFGYTINDEKGKP
TNSEFIALIADKLRLGMKAG

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=109, Percent_Identity=36.697247706422, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 29153; Mature: 29153

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKIRVGIADDNKEFATILAEYLSTQENIEVVGVANDGNQAIELIKNQSPDLLILDIIM
CCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCEEHHHHHH
PYLDGIGVLEKINEEQLKKPKILVLSAVGQEKITQRAIALGADYYILKPFDLAMLSKRIS
HHHCCCHHHHHCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHH
ELMEPELNVVSKTVFPVVKAKKNYDLEAMITEIIHEVGVPAHIKGYMYLRDAISLVISNM
HHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EYLNSVTKMLYPKIAEKYNTTPSRVERAIRHAIEVAWSRGKTEVLNELFGYTINDEKGKP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCEEECCCCCCC
TNSEFIALIADKLRLGMKAG
CCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVKKIRVGIADDNKEFATILAEYLSTQENIEVVGVANDGNQAIELIKNQSPDLLILDIIM
CCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCEEHHHHHH
PYLDGIGVLEKINEEQLKKPKILVLSAVGQEKITQRAIALGADYYILKPFDLAMLSKRIS
HHHCCCHHHHHCCHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHH
ELMEPELNVVSKTVFPVVKAKKNYDLEAMITEIIHEVGVPAHIKGYMYLRDAISLVISNM
HHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EYLNSVTKMLYPKIAEKYNTTPSRVERAIRHAIEVAWSRGKTEVLNELFGYTINDEKGKP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCEEECCCCCCC
TNSEFIALIADKLRLGMKAG
CCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7885226 [H]